scholarly journals Use of Amino Oligosaccharins and Alternaria Fine Protein in the Management of Crown Gall Disease in Roses

2020 ◽  
Vol 8 (2) ◽  
pp. 118-127
Author(s):  
Emmanuel Wesonga Atsango ◽  
William Maina Muiru ◽  
Agnes Wakesho Mwang’ombe ◽  
Liu Gaoqiong
Keyword(s):  
Crown Gall ◽  
Block Design ◽  
Foliar Spray ◽  
Negative Control ◽  
Rosa Hybrida ◽  
Nutrient Agar ◽  
Biochemical Tests ◽  
Crown Gall Disease ◽  
Pests And Diseases ◽  
Agar Media

Rose (Rosa hybrida Vill.) production is limited by a variety of factors such as poor mineral nutrition, high salinity, pests and diseases. Crown gall disease caused by Agrobacterium tumefaciens causes a significant damage to roses in Kenya. The study was carried out in Winchester farm (Nairobi) and Bahati farm (Nakuru) to determine the effects of a mixture of amino oligosaccharins and Alternaria fine proteins on crown gall disease in roses. The experiment was conducted on Rosa hybrida var. Mariyo in a Randomized Complete Block Design with four replications. The treatments comprised of different rates 0.5, 1, 1.5g/L of water of the product at 3% concentration applied as foliar spray and a commonly used product copper sulphate pentahydrate (Mastercop) produced by ADAMA applied at 2ml/ L as the standard and water as a negative control. Crown gall tissues were collected from four different roses per treatment from the two sites, counted and used for biochemical tests in Kabete laboratory. Bacteria were isolated by culturing and the representative colony types growing on nutrient agar media selected and sub-cultured by successive streaking on nutrient agar media. The biochemical test for the different isolates was done to identify the bacterial isolates. Application of the mixture of amino oligosaccharins and Alternaria activated protein at the rate of 1.5g/L had significant effects on galls formation and reduced the numbers significantly at both farms. The gram reaction indicated that the selected isolates were gram negative and were positive for motility, catalase, oxidase, lactose, mannitol, and salt tolerance tests. There was a significant reduction in the number of galls and size following the application of amino oligosaccharins and Alternaria fine proteins as well as improvement in plant growth. The ability to manage the disease can be attributed to enhanced defense enzyme activity enhanced by amino oligosaccharins and Alternaria fine proteins.

scholarly journals CHARACTERIZATION OF BACTERIA ASSOCIATED WITH PNEUMONIA IN BLACK BENGAL GOATS

2012 ◽  
Vol 9 (1) ◽  
pp. 67-71 ◽  
Author(s):  
MA Momin ◽  
MA Islam ◽  
MM Khatun ◽  
MM Rahman ◽  
MA Islam
Keyword(s):  
Antimicrobial Agents ◽  
Research Work ◽  
Nutrient Agar ◽  
Blood Agar ◽  
Biochemical Tests ◽  
Highly Sensitive ◽  
Golden Yellow ◽  
Agar Media ◽  
Staphylococcus Spp

The present research work was undertaken for the characterization of the bacterial pathogens responsible for pneumonia in black Bengal goats. Nasal swab samples (n = 50) were collected from the pneumonic black Bengal goats in Mymensingh and Sirajgonj districts. Samples were inoculated onto nutrient agar, eosin methylene blue (EMB) agar, MacConkey agar, and blood agar media for isolation of bacteria. Identification of bacteria was performed by the Gram's staining method, cultural properties and biochemical tests. Antibiotic sensitivity of bacterial isolates was performed against 11 antimicrobial agents. Pasteurella spp were isolated from 25 cases, and Staphylococcus spp from 13 cases. Mixed infection caused by the Pasteurella spp and Staphylococcus spp. were recorded in 12 cases. Pasteurella spp produced whitish, opaque circular and translucent colonies on nutrient agar, smooth, convex, glistening colonies on EMB agar and no hemolysis on blood agar. Staphylococcus spp have shown gray white or golden yellowish colonies on  nutrient agar. Golden yellow colonies without hemolysis or whitish colonies with hemolysis were also produced by Staphylococcus spp. on the blood agar media. Pasteurella spp were indole positive, MR-VP negative and ferment dextrose, sucrose and mannitol with the production of acid. The Staphylococcus spp were positive to MR-VP, coagulase and catalase reactions, negative to indole test and fermented five basic sugars with acid production. Results of cultural and biochemical tests supported that these two isolates belonged to P. multocida and S. aureus. P. multocida were highly sensitive to ciprofloxacin and resistant to penicillin. S. aureus found to be highly sensitive to erythromycin, tetracycline, enrofloxacin, and norfloxacin and less sensitive to amoxicillin. DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11215Bangl. J. Vet. Med. (2011). 9(1): 67-71 

scholarly journals Cultivar, Plant Height and Fungicide for Integrated Control of Ramularia Spot and Increased Cotton Yield

2020 ◽  
Vol 8 (2) ◽  
pp. 224
Author(s):  
Hugo Manoel De Souza ◽  
Alfredo Ricieri Dias ◽  
Gustavo De Faria Theodoro ◽  
Christian Rones Wruck de Souza Osório ◽  
Fernando Fagner Magalhães
Keyword(s):  
Plant Height ◽  
Integrated Control ◽  
Block Design ◽  
Economic Value ◽  
Total Yield ◽  
Negative Control ◽  
Cotton Yield ◽  
Pests And Diseases ◽  
Progress Curve ◽  
Randomized Complete Block Design

Cotton is a crop with a high economic value, usually sprayed with large amounts of pesticides to control pests and diseases. Ramularia spot is a foliar disease that cause early defoliation and can compromise cotton yield; however, there is a need of information about the interaction of cultivars and plant height as a tool to improve ramularia control and increase yield. The objective of this study was to evaluate the effects of the interaction between plant height, cultivar, and fungicides applied to the severity of the leaf spot and cotton yield. The experiment was carried on during the 2014/15 crop season in Chapadão do Sul, MS, Brazil. A randomized complete block design in a 2 × 3 × 3 factorial scheme was used, where the factors were plant heights (1 and 1.5 m), cultivar (FMT 701, FM 975, and FM 944) and type of fungicide (Negative control , FT1, and FT2). The fungicidal treatments consisted of sequential sprays of different fungicides, including the triazole, strobilurin, and carboxamide groups, recommended to farmers. The cultivar FM 975 showed greater ramularia spot progress curve when grown at 1 m height and without fungicide spray. The fungicides were efficient in reducing the severity of the ramularia spot and their use in shorter plants (1 m) increased yield. The cultivar FM 975 had the highest total yield.

scholarly journals Occurrence of Crown Gall Disease on Japanese Spindle (Euonymus japonicas var. Green Rocket) Caused by Agrobacterium rosae in Iran

Plant Disease ◽  
2021 ◽  
Author(s):  
Hamzeh Mafakheri ◽  
Seied Mohsen Taghavi ◽  
Sadegh Zarei ◽  
Nemanja Kuzmanovic ◽  
Ebrahim Osdaghi
Keyword(s):  
Crown Gall ◽  
De Novo ◽  
Negative Control ◽  
Whole Genome Sequence ◽  
Reca Gene ◽  
Post Inoculation ◽  
Whole Genome ◽  
Bacterial Strains ◽  
Illumina Hiseq ◽  
Crown Gall Disease

Crown gall disease caused by diverse Agrobacterium species is one of the main biotic constraints in the ornamental plants industry in Iran (Mafakheri et al. 2017). In August 2019, Japanese spindle (Euonymus japonicus var. Green Rocket) plants showing crown gall symptoms were observed in a commercial greenhouse in Tehran, Iran. Infected plants were characterized by a visible overgrowth on their stems and crown. Bacterial isolation from the gall tissues was performed on nutrient agar (NA) and 1A media as described by Moore et al. (2001). The six resulted bacterial strains (A.E1 to A.E6) were evaluated using PCR primer pair F8360/F8361 amplifying a 453 bp DNA fragment in recA gene and confirmed as Agrobacterium sp. (Shams et al. 2013). Pathogenicity of the strains was evaluated in two independent assays on Japanese spindle plantlets as well as 10-15 day old tomato (Solanum lycopersicum cv. Sunseed 6189) and sunflower (Helianthus annuus cv. Armavirski) plants in greenhouse conditions using the needle prick method as described previously (Mafakheri et al. 2019). The reference strain A. radiobacter ICMP 5856 and sterile distilled water were used as positive and negative controls, respectively. Crown gall symptoms appeared 20-25 days post inoculation on the Japanese spindle plantlets as well as tomato and sunflower plants inoculated with the strains isolated in this study, while the negative control plants remained asymptomatic. Koch’s postulates were accomplished by re-isolating on NA medium and PCR-based identification of the inoculated strains from the symptomatic plants. The representative strain A.E1 was subjected to multilocus sequence analysis (MLSA) using the sequences of four housekeeping genes (i.e. atpD, gyrB, recA, and rpoB) as described previously (Mafakheri et al. 2019). MLSA results revealed that the strain A.E1 is phylogenetically closely related to A. rosae. The sequences were deposited into GenBank under the accession numbers MT007962 to MT007965 for atpD, gyrB, recA, and rpoB, respectively. Further, the strain A.E1 was subjected to whole genome sequencing using Illumina HiSeq X platform. DNA extraction was performed using NucleoSpin Microbial DNA kit (Macherey-Nagel, Germany), DNA libraries were obtained with Nextera XT DNA Library Prep Kit (Illumina, USA), and de novo sequence assembly was performed using SPAdes genome assembler. The resulting whole genome sequence was deposited into the GenBank database under the accession number JAFJZW000000000. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values were calculated among all the type strains of Agrobacterium species/genomospecies using standard criteria as detailed previously (Osdaghi et al. 2020; Chen et al. 2021). The strain A.E1 had 97% ANI and 72% dDDH values with A. rosae strain NCPPB 1650, suggesting that the bacterial strains isolated from Japanese spindle in Iran belong to A. rosae. This is the first report of A. rosae causing crown gall disease on Japanese spindle in Iran. The new crown gall disease could negatively affect the ornamental shrub production industry in central Iran unless strict sanitary measures are taken into the account in the nurseries in these areas. Further nationwide surveys and samplings are warranted to elucidate the economic impact of the pathogen on ornamental plant industry in the country.

scholarly journals Daya bunuh dan daya hambat antimikrobial chlorhexidine 2% dan povidone iodine 1% sebagai medikamen saluran akar terhadap Enterococcus faecalis The ability of chlorhexidine 2% and povidone iodine 1% as root canal medicaments to kill and inhibit Enterococcus faecalis

2019 ◽  
Vol 7 (1) ◽  
pp. 12
Author(s):  
Nathania Astria ◽  
Ari Subiyanto ◽  
Latief Mooduto
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Povidone Iodine ◽  
Inhibition Zone ◽  
Colony Growth ◽  
Negative Control ◽  
Nutrient Agar ◽  
Diffusion Method ◽  
The Media ◽  
Agar Media

Background. Enterococcus faecalis is one of the pathogenic organisms associated with root canal treatment failure and apical periodontitis. Root canal medicament  is needed to prevent re-infection in the root canal and increase the success of treatment. Chlorhexidine and povidone iodine is a broad spectrum root canal medicaments that can kill gram-positive bacteria. Purpose. The purpose of this study was to discover the ability to kill and inhibit of antimicrobial chlorhexidine 2% and 1% povidone iodine asvroot canal medicaments against bacteria Enterococcus faecalis. Methode. This research was done by measuring the inhibition zone and count the number of colonies. Determination of the inhibition of root canal medicaments against Enterococcus faecalis by diffusion method. 10 microliter root canal medicaments dropped on paperdisk and placed on nutrient agar media with enterococcus faecalis, then inhibition zone was calculated. Determination ability to kill enterococcus faecalis is done by inserting 1 ml medicaments root canal into 5 ml BHIB media, then 0.05 ml inoculum of Enterococcus faecalis inserted into each tube, except the negative control. 0.1 ml of each tube implanted in the media nutrient agar. Media incubated for 24 hours, then Enterococcus faecalis bacterial colonies that grow in media calculated using the CFU. Results. There no colony growth of enterococcus faecalis in both root canal medicaments. There are significant differences in inhibition zone of 2% chlorhexidine and 1% povidone iodine (p<0.05). Conclusion. Both of root canal medicaments can kill enterococcus faecalis, but chlorhexidine 2% was more capable inhibit Enterococcus faecalis.

scholarly journals Identification of Agrobacterium vitis as a causal agent of grapevine crown gall in Serbia

2012 ◽  
Vol 64 (4) ◽  
pp. 1487-1494 ◽  
Author(s):  
N. Kuzmanovic ◽  
Katarina Gasic ◽  
M. Ivanovic ◽  
Andjelka Prokic ◽  
A. Obradovic
Keyword(s):  
Crown Gall ◽  
23S Rrna ◽  
Vitis Vinifera L ◽  
Rrna Gene ◽  
Agrobacterium Vitis ◽  
Biochemical Tests ◽  
Crown Gall Disease ◽  
Multiplex Pcr Assay ◽  
Pcr Product ◽  
23S Rrna Gene

In 2010, a serious outbreak of crown gall disease was observed on grapevines (Vitis vinifera L. cv. Cabernet Sauvignon) in several commercial vineyards located in the Vojvodina province, Serbia. Bacteria were isolated from the young tumor tissue on nonselective YMA medium and five representative strains were selected for further identification. Tumorigenic (Ti) plasmid was detected in all strains by PCR using primers designed to amplify the virC pathogenicity gene, producing a 414-bp PCR product. The strains were identified as Agrobacterium vitis using differential physiological and biochemical tests, and a multiplex PCR assay targeting 23S rRNA gene sequences. In the pathogenicity assay, all strains induced characteristic symptoms on inoculated tomato and grapevine plants. They were less virulent on tomato plants in comparison to the reference strains of A. tumefaciens and A. vitis.

scholarly journals Molybdenum mixed with glyphosate and alone via foliar spray in no-tillage common bean grown on corn stover

Revista CERES ◽  
2014 ◽  
Vol 61 (1) ◽  
pp. 62-69
Author(s):  
Gessimar Nunes Camelo ◽  
Geraldo Antonio de Andrade Araújo ◽  
Renildes Lucio Ferreira Fontes ◽  
Luiz Antonio dos Santos Dias ◽  
José Eustáquio de Souza Carneiro ◽  
...  
Keyword(s):  
Common Bean ◽  
Corn Stover ◽  
Field Experiments ◽  
Block Design ◽  
Foliar Spray ◽  
Cumulative Effect ◽  
No Tillage ◽  
Randomized Block Design ◽  
Tillage System ◽  
Mo Content

The effect of molybdenum (Mo) on common bean grown in desiccated corn stover in a no-tillage system was evaluated under two application modes: Mo mixed with the desiccant glyphosate and Mo direct spray to the bean leaves. The treatments (four replicates) were assigned to a completely randomized block design in a split-plot arrangement with the application of Mo (0, 100, 200, 400 and 800 g ha-1) mixed with glyphosate in the main plots and Mo foliar spray (0 and 100 g ha-1) in the sub-plots. The field experiments were carried out in 2009 and 2010 in the municipality of Coimbra, Minas Gerais State, with the common bean cultivar Ouro Vermelho. Mo mixed with glyphosate had neither an effect on common bean yield nor on the Mo and N contents in leaves, however it increased the Mo and N contents in seeds. Application of Mo via foliar spray increased Mo content in leaves and Mo and N contents in seeds. The reapplication of molybdenum with glyphosate for desiccation in subsequent crops caused a cumulative effect of Mo content in bean seeds.

scholarly journals CHARACTERIZATION AND ANTIMICROBIAL SPECTRUM OF A POTENT STREPTOMYCES SP. GOS2 ISOLATED FROM WESTERN GHATS OF KARNATAKA, INDIA

2016 ◽  
Vol 8 (9) ◽  
pp. 310
Author(s):  
Gautham S. A. ◽  
Sunita C. Mesta ◽  
Onkarappa R.
Keyword(s):  
Western Ghats ◽  
Uv Spectroscopy ◽  
Aerial Mycelium ◽  
Biochemical Tests ◽  
Antimicrobial Spectrum ◽  
Antibiotic Resistant ◽  
Test Organisms ◽  
Carbohydrate Fermentation ◽  
Agar Media ◽  
Spectroscopy Studies

<p><strong>Objective: </strong>Western Ghats of Karnataka, India-a biodiversity hotspot is a rich source of microorganisms with undiscovered metabolic capabilities. The upsurge of antibiotic-resistant pathogens has created a greater demand for novel antibiotics. In context to the above a potent<em> </em>soil<em> Streptomyces</em> sp. GOS2 isolated from Agumbe regions of Western Ghats, and its metabolite was assessed and characterized for its antimicrobial spectrum.</p><p><strong>Methods: </strong>The isolation was carried out by soil serial dilution plating on Starch Casein Nitrate agar media (SCN). The obtained isolate was characterized by morphological and biochemical tests. The antimicrobial activity was assessed by well in agar methods against 28 test organisms. The partial characterization of the bioactive metabolite was carried out by thin layer chromatography (TLC) and UV–Visible spectroscopy studies.</p><p><strong>Results: </strong>The isolated GOS2 was observed as a raised powdery colony with grey colored aerial mycelium and media impregnated substrate mycelium. The spore chain was rectus with smooth spore surface. The isolate was gram positive, non-acid fast, positive for catalase, hydrogen sulphide production and starch hydrolysis, negative for casein and gelatin hydrolysis. The carbohydrate fermentation studies showed acid production in dextrose and alkali production in sucrose, lactose, maltose and starch. A prominent antibacterial activity was observed with a zone of inhibition measuring 21-27 mm. The TLC showed a purple spot and UV spectroscopy revealed λ max at 233.2 and 235 nm indicating macrolide group of antibiotics.</p><p><strong>Conclusion: </strong>Western Ghats actinomycetes are a potent source of novel antibiotic molecules.</p>

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