scholarly journals IDENTIFIKASI BAKTERI AEROB DI UDARA RUANG OPERASI INSTALASI BEDAH SENTRAL (IBS) RSUP ROF. DR. R. D. KANDOU MANADO

2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Billy V. Palawe ◽  
Constantien Kountul ◽  
Olivia Waworuntu
Keyword(s):  
Bacillus Subtilis ◽  
Operating Room ◽  
Descriptive Study ◽  
Aspergillus Species ◽  
Nutrient Agar ◽  
Blood Agar ◽  
Aerobic Bacteria ◽  
Particulate Matters ◽  
Water Droplets ◽  
Agar Media

Abstract: Air is not a medium where microbes grow, but it is a carrier of particulate matters, such as dust and water droplets which are very probably loaded with microbes. And the most of bacteria that live in the air of the hospitals room environment consist of Bacillus bacteria, E.coli, Staphylococcus, Streptococcus, Pseudemonas, also fungi which is Aspergillus species. Objective: To determine the existence of aerobic bacteria and others species in the operating room of air Installation Central Surgery (IBS). Prof. Dr. R. D. Kandou Manado. Methods: It is a prospective descriptive study to get an overview of the bacteria in the air of aerobic Central Surgery Installation operating room (IBS) Prof. Dr. R. D. Kandou Manado. Results: Staphylococcus Albus bacteria found in Nutrient Agar media (NA) And Blood Agar (AD), while Bacillus subtilis found in Blood Agar media (AD).Conclusion: In the air of Central Surgery Installation operations room (IBS) Prof. Dr. RD Kandou Manado, based on the result shows the high number of aerobic bacteria.Keywords: aerobic bacteria,in the operations room air.Abstrak: Udara bukan merupakan medium tempat mikroba tumbuh, tetapi merupakan pembawa bahan partikulat, debu, dan tetesan air yang semuanya sangat mungkin dimuati mikroba. Mikroorganisme yang paling banyak berkeliaran di udara lingkungan rumah sakit adalah bakteri Bacillus, E.coli, Staphylococcus, Streptococcus, Pseudemonas, dan spesies jamur seperti Aspergillus. Tujuan: Mengetahui adanya bakteri aerob dan jenis lainnya di udara ruang operasi Instalasi Bedah Sentral (IBS) RSUP. Prof. Dr. R. D. Kandou Manado. Metode: Penelitian ini bersifat penelitian deskriptif prospektif yaitu untuk mendapatkan gambaran bakteri aeorob di udara ruang operasi Instalasi Bedah Sentral (IBS) RSUP Prof. Dr. R. D. Kandou Manado. Hasil: Staphylococcus Albus ditemukan di media spesifik Agar Nutrientt (NA) dan Agar Darah (AD) sedangkan Bacillus Subtilis ditemukan di media spesifik Agar Darah (AD). Simpulan: Udara diruang operasi Instalasi Bedah Sentral (IBS) RSUP Prof. Dr.R. D. Kandou Manado, menunjukan masih tingginya jumlah angka bakteri aerob.Kata kunci: bakteri aerob, udara diruang operasi.

scholarly journals POLA BAKTERI AEROB YANG BERPOTENSI MENYEBABKAN INFEKSI NOSOKOMIAL DI KAMAR OPERASI CITO BLU RSUP PROF. Dr. R. D. KANDOU MANADO

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Arlita F. Sofyan ◽  
Heriyannis Homenta ◽  
Fredine Rares
Keyword(s):  
Bacillus Subtilis ◽  
Nosocomial Infection ◽  
Hospital Admission ◽  
Operating Room ◽  
Ambient Air ◽  
Descriptive Study ◽  
Aerobic Bacteria ◽  
Bacterial Identification ◽  
Gram Negative ◽  
Emergency Operating

Abstract: Nosocomial infection is an infection acquired or experienced by patients during hospitalized and the new symptoms of infection appear after 72 hours hospitalized and this infection is not found in patients upon hospital admission. This study aimed to determine the pattern of nosocomial infection-causing bacteria in the cito operating room at Hospital of Prrof. Dr. R. D. Kandou Manado. This was a descriptive study with a prospective approach. Samples were taken from the walls, floors, and the ambient air, followed by bacterial identification tests. The results showed that from 7 samples taken from the walls, 5 samples (74.42%) contained Bacillus subtilis; from 9 samples taken from the floors 3 samples (30%) had Bacillus subtilis as well as Gram-negative cocci in 3 samples (30%); from 12 samples taken from the air, 7 samples (58.33%) contained Gram-positive cocci.Keywords: aerobic bacteria, nosocomial infection, CITO (emergency) operating roomAbstrak: Infeksi nosokomial adalah suatu infeksi yang diperoleh atau dialami oleh pasien selama dirawat di rumah sakit dan menunjukkan gejala infeksi baru setelah 72 jam pasien berada di rumah sakit serta infeksi itu tidak ditemukan saat pasien masuk ke rumah sakit. Penelitian ini bertujuan untuk mengetahui pola bakteri penyebab infeksi nosokomial yang terdapat di kamar operasi cito BLU RSUP Prof. Dr. R. D. Kandou Manado. Desain penelitian bersifat deskriptif dengan pendekatan prospektif. Sampel penelitian diambil dari dinding, lantai dan udara di kamar operasi kemudian dilakukan uji identifikasi bakteri. Hasil penelitian mmperlihatkan pada 7 sampel dinding ditemukan terbanyak yaitu Bacillus subtillis sebanyak 5 sampel (74,42%); pada 9 sampel lantai ditemukan terbanyak yaitu Bacillus subtillis sebanyak 3 sampel (30%) dan kokus gram negatif sebanyak 3 sampel (30%); pada 12 sampel udara ditemukan bakteri terbanyak yaitu kokus gram positif sebanyak 7 sampel (58,33%).Kata kunci: bakteri aerob, infeksi nosokomial, kamar operasi cito

scholarly journals CHARACTERIZATION OF BACTERIA ASSOCIATED WITH PNEUMONIA IN BLACK BENGAL GOATS

2012 ◽  
Vol 9 (1) ◽  
pp. 67-71 ◽  
Author(s):  
MA Momin ◽  
MA Islam ◽  
MM Khatun ◽  
MM Rahman ◽  
MA Islam
Keyword(s):  
Antimicrobial Agents ◽  
Research Work ◽  
Nutrient Agar ◽  
Blood Agar ◽  
Biochemical Tests ◽  
Highly Sensitive ◽  
Golden Yellow ◽  
Agar Media ◽  
Staphylococcus Spp

The present research work was undertaken for the characterization of the bacterial pathogens responsible for pneumonia in black Bengal goats. Nasal swab samples (n = 50) were collected from the pneumonic black Bengal goats in Mymensingh and Sirajgonj districts. Samples were inoculated onto nutrient agar, eosin methylene blue (EMB) agar, MacConkey agar, and blood agar media for isolation of bacteria. Identification of bacteria was performed by the Gram's staining method, cultural properties and biochemical tests. Antibiotic sensitivity of bacterial isolates was performed against 11 antimicrobial agents. Pasteurella spp were isolated from 25 cases, and Staphylococcus spp from 13 cases. Mixed infection caused by the Pasteurella spp and Staphylococcus spp. were recorded in 12 cases. Pasteurella spp produced whitish, opaque circular and translucent colonies on nutrient agar, smooth, convex, glistening colonies on EMB agar and no hemolysis on blood agar. Staphylococcus spp have shown gray white or golden yellowish colonies on  nutrient agar. Golden yellow colonies without hemolysis or whitish colonies with hemolysis were also produced by Staphylococcus spp. on the blood agar media. Pasteurella spp were indole positive, MR-VP negative and ferment dextrose, sucrose and mannitol with the production of acid. The Staphylococcus spp were positive to MR-VP, coagulase and catalase reactions, negative to indole test and fermented five basic sugars with acid production. Results of cultural and biochemical tests supported that these two isolates belonged to P. multocida and S. aureus. P. multocida were highly sensitive to ciprofloxacin and resistant to penicillin. S. aureus found to be highly sensitive to erythromycin, tetracycline, enrofloxacin, and norfloxacin and less sensitive to amoxicillin. DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11215Bangl. J. Vet. Med. (2011). 9(1): 67-71 

Studies on Trypanosoma avium

Parasitology ◽  
1966 ◽  
Vol 56 (1) ◽  
pp. 15-19 ◽  
Author(s):  
J. R. Baker
Keyword(s):  
Nutrient Agar ◽  
Blood Agar ◽  
Insect Vector ◽  
Corvus Frugilegus ◽  
Agar Media

A strain of Trypanosoma avium isolated from Corvus frugilegus in England was readily cultivated in vitro in diphasic blood agar, or blood nutrient agar, media at 28 °C.In both media, small trypanosomes appeared which morphologically resembled the metacyclic forms from the insect vector and which were infective to canaries. They were more numerous, and developed more regularly, in the medium with the nutrient agar base.At 40–41 °C a few of these small trypanosomes apparently developed into large trypanosomes similar to, but not identical with, those seen in the blood of infected birds.

scholarly journals POLA BAKTERI AEROB PADA SPUTUM PETUGAS PENYAPU JALAN YANG MENDERITA INFEKSI SALURAN PERNAPASAN DI KOTA MANADO

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Michael A. Tumiwa ◽  
Standy Soeliongan ◽  
Olivia Waworuntu
Keyword(s):  
Bacillus Subtilis ◽  
Respiratory Tract ◽  
Acute Respiratory Infection ◽  
Respiratory Diseases ◽  
Respiratory Tract Infections ◽  
Descriptive Study ◽  
Aerobic Bacteria ◽  
Common Disease ◽  
Proteus Vulgaris ◽  
Tract Infections

Abtract: Respiratory tract infection is a common disease with mild to severe manifestations. Acute respiratory infection can be transmitted by saliva, blood, sneezing, breathing air containing bacteria which are inhaled by healthy people. Respiratory diseases will increase in relation to the profession and the environment, such as street sweepers. This study aimed to determine the pattern of aerobic bacteria in the sputum of street sweepers who suffered respiratory tract infections in Manado. This was a descriptive study. The results showed that Bacillus subtilis was obtained in 1 sample (4.76%), Streptococcuss spp. in 6 samples (30%), Providence stuarti in 3 samples (14.28%), Diplococcus in 2 samples (9.52%), Klebsiela pneumonie in 3 samples (14.28%), Proteus vulgaris in 4 samples (19.04%), Enterobacter aeorogenes in 1 sample (4.76%), and Staphylococcus in 1 sample (4.76%). Conclusion: Streptococcuss spp. was the most commonly bacteria found in the sputum of street sweepers in Manado.Keywords: aerobic bacteria, sputum, street sweepersAbstrak: Penyakit infeksi saluran pernapasan merupakan penyakit yang sering dijumpai dengan manifestasi ringan sampai berat. Infeksi saluran pernapasan akut dapat di tularkan melalui air ludah, darah, bersin, serta udara pernapasan yang mengandung kuman yang terhirup oleh orang sehat. Pada petugas penyapu jalan, penyakit-penyakit saluran pernafasan akan lebih meningkat berhubung dengan profesi dan lingkungan kerjannya. Penelitian ini bertujuan untuk mengetahui pola bakteri aerob pada sputum petugas penyapu jalan yang menderita infeksi saluran pernafasan di Kota Manado. Penelitian ini menggunakan metode deskriptif. Hasil penelitian menunjukkan ditemukannya Basil subtilis pada 1 sampel (4,76%), Streptococcuss spp. pada 6 sampel (30%), Providensia stuarti pada 3 sampel (14,28%), Diplococcus pada 2 sampel (9,52%), Klebsiela pneumonie pada 3 sampel (14,28%), Proteus vulgaris pada 4 sampel (19,04%), Enterobacter aeorogenes pada 1 sampel (4,76%), dan Staphylococcus pada 1 sampel (4,76%). Simpulan: Streptococcuss spp. merupakan bakteri yang paling banyak ditemukan pada sputum penyapu jalan di Kota Manado.Kata kunci: bakteri aerob, sputum, penyapu jalan.

scholarly journals Pola Bakteri Aerob yang Berpotensi Menyebabkan Infeksi Nosokomial di Ruang Instalasi Bedah Sentral (IBS) RSUP Prof. Dr. R.D. Kandou Manado

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Tanya P. M. Putri ◽  
Olivia A. Waworuntu ◽  
Fredine E. S. Rares
Keyword(s):  
Bacillus Subtilis ◽  
Public Education ◽  
Nosocomial Infections ◽  
Medical Equipment ◽  
Descriptive Study ◽  
Aerobic Bacteria ◽  
Klebsiella Pneumonia ◽  
Bacillus Sp ◽  
Proteus Vulgaris ◽  
Air Samples

Abstract: Nosocomial infections can be interpreted as infections that originate or could occur in hospitals and arise within 48 hours after being hospitalized up to 30 days patients off care. In Indonesia, namely in 10 Public Education Hospitals, the nosocomial infections percentage is 6-16% with an average of 9.8% in 2010. This study was aimed to identifity the source and the bacteria pattern that caused nosocomial infections in the Intallation of Central Surgery Room (IBS) of Prof. Dr. R. D. Kandou Hospital Manado. This was a descriptive study with a prospective approach. Samples were bacteria on the wall, floor, air, furniture, and medical equipment in the IBS room of Prof. Dr. R. D. Kandou Hospital Manado. The results showed that of 4 wall samples, the most common bacteria were Bacillus sp. and Klebsiella pneumonia, each in 2 samples (50%), Of the 5 floor samples, the most common bacteria were Staphylococcus sp. in 2 samples (40%). Of the 15 air samples, the most common bacteria was Bacillus sp. in 7 samples (46.7%). Of the 3 samples of medical equipment, the most common bacteria were Proteus vulgaris in 1 sample (33.3%), Bacillus sp. in 1 sample (33.3%), and Diplococcus in 1 sample (33.%). Conclusion: Bacillus sp. is the most common bacteria found in IBS room of Prof. Dr. R. D. Kandou Hospital Manado.Keywords: pattern of aerobic bacteria, nosocomial infections, central surgery room Abstrak: Infeksi nosokomial adalah infeksi yang berasal atau terjadi di rumah sakit yang timbul dalam kurun waktu 48 jam setelah dirawat di rumah sakit sampai dengan 30 hari lepas rawat. Di 10 RSU pendidikan di Indonesia didapatkan kejadian infeksi nosokomial cukup tinggi yaitu 6-16% dengan rerata 9,8% pada tahun 2010. Penelitian ini bertujuan untuk mengetahui sumber dan pola bakteri penyebab infeksi nosokomial yang terdapat diruang Instalasi Bedah Sentral (IBS) RSUP Prof. Dr. R. D. Kandou Manado. Jenis penelitian ialah deskriptif dengan pendekatan prospektif. Sampel penelitian ialah bakteri pada dinding, lantai, udara, perabotan, dan peralatan medis di ruang IBS RSUP Prof. Dr. R. D. Kandou Manado. Hasil penelitian memperlihatkan dari 4 sampel dinding, bakteri terbanyak yaitu Bacillus sp. dan Klebsiella pneumonia, masing-masing 2 sampel (50%); dari 5 sampel lantai, bakteri terbanyak yaitu Staphylococcus sp. pada 2 sampel (40%); dari 15 sampel udara, bakteri terbanyak yaitu Bacillus sp. pada 7 sampel (46,7%). Dari 3 sampel peralatan medis didapatkan bakteri terbanyak yaitu Proteus vulgaris pada 1 sampel (33,3%), Bacillus subtilis pada 1 sampel (33,3%), dan Diplococcus pada 1 sampel (33,3%). Simpulan: Bacillus sp. merupakan bakteri yang terbanyak ditemukan.Kata kunci: pola bakteri aerob, infeksi nosokomial, ruang instalasi bedah sentral (IBS)

Evaluation of Microbial Load from Canned Soya Milk Drinks in Malaysia

2016 ◽  
Vol 2 (1) ◽  
pp. 22-25
Author(s):  
Nur Amalina binti Mustafa ◽  
Muhammad Ashraf bin Redzuan ◽  
Muhamad Hazim bin Zuraimi ◽  
Muhamad Shuhaimi bin Shuib ◽  
Shahnaz Majeed ◽  
...  
Keyword(s):  
Culture Media ◽  
Storage Condition ◽  
Storage Conditions ◽  
Nutrient Agar ◽  
Microbial Load ◽  
Blood Agar ◽  
Human Consumption ◽  
Processing Unit ◽  
Soya Milk ◽  
Two Samples

Objective: Owing to the habit of consuming ready food among the citizens of Malaysia a study was conducted to evaluate 20 samples of canned soya milk for the presence of possible microbial content. The samples were collected randomly from shopping malls, restaurants and kiosk in Ipoh Malaysia. Methods: All samples collected across Ipoh, were subjected to test for presence bacteria in nutrient agar, blood agar and macConkey media. The possible microbial load was swapped from surface and soya milk content with a sterile cotton and streaked on nutrient agar, blood agar and macConkey culture media. The streaked petri plates were incubated for 48 hours at 37oC. Results: The study revealed negative microbial growth in all except two samples from the surface and soya milk content collected from a restaurant in nutrient agar and blood agar medium. The presence of microbes was conformed as gram positive staphylococcus sp. through gram staining. The positive growth may be imputed to poor storage condition at the restaurant. Conclusion: It can be computed from the study that the majority of the samples were free from bacterial growth, suggesting strong in house quality control mechanism at the processing unit and exquisite storage conditions in malls and kiosk suggesting that soya milk available in malls and kiosk are fit for human consumption.

Antagonistic activity of Bacillus subtilis strains isolated from various sources

2018 ◽  
Vol 8 (2) ◽  
pp. 354-364
Author(s):  
A. N. Irkitova ◽  
A. V. Grebenshchikova ◽  
A. V. Matsyura
Keyword(s):  
Escherichia Coli ◽  
Bacillus Subtilis ◽  
Wild Strain ◽  
Fish Farming ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
E Coli ◽  
Long Period ◽  
Test Culture ◽  
Agar Media

<p>An important link in solving the problem of healthy food is the intensification of the livestock, poultry and fish farming, which is possible only in the adoption and rigorous implementation of the concept of rational feeding of animals. In the implementation of this concept required is the application of probiotic preparations. Currently, there is an increased interest in spore probiotics. In many ways, this can be explained by the fact that they use no vegetative forms of the bacilli and their spores. This property provides spore probiotics a number of advantages: they are not whimsical, easily could be selected, cultivated, and dried. Moreover, they are resistant to various factors and could remain viable during a long period. One of the most famous spore microorganisms, which are widely used in agriculture, is <em>Bacillus subtilis</em>. Among the requirements imposed to probiotic microorganisms is mandatory – antagonistic activity to pathogenic and conditional-pathogenic microflora. The article presents the results of the analysis of antagonistic activity of collection strains of <em>B. subtilis</em>, and strains isolated from commercial preparations. We studied the antagonistic activity on agar and liquid nutrient medias to trigger different antagonism mechanisms of <em>B. subtilis</em>. On agar media, we applied three diffusion methods: perpendicular bands, agar blocks, agar wells. We also applied the method of co-incubating the test culture (<em>Escherichia coli</em>) and the antagonist (or its supernatant) in the nutrient broth. Our results demonstrated that all our explored strains of <em>B. subtilis</em> have antimicrobial activity against a wild strain of <em>E. coli</em>, but to varying degrees. We identified strains of <em>B. subtilis</em> with the highest antagonistic effect that can be recommended for inclusion in microbial preparations for agriculture.</p><p><em><br /></em><em></em></p>

scholarly journals IDENTIFIKASI BAKTERI AEROB PADA URIN PORSI TENGAH PASIEN PENYAKIT GINJAL KRONIK STADIUM 5 DI BLU RSUP PROF. R.D. KANDOU MANADO

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Nur Dinah Mamonto ◽  
Standy Soeliongan ◽  
Heriyannis Homenta
Keyword(s):  
Bacillus Subtilis ◽  
Urine Culture ◽  
Aerobic Bacteria ◽  
Defense System ◽  
Biochemical Tests ◽  
Normal People ◽  
Increased Risk ◽  
Ckd Stage ◽  
High Incidence ◽  
Descriptive Method

Abstract: Chronic kidney disease (CKD) is a chronic disease that can lead to some abnormality in the body's defense system which allows an increased risk of infection, one urinary tract infection. In patients with CKD stage 5, impaired immunological function and infections often occur. A high incidence of infection found in uremic patients. Uremia suppress the function of most of the patient's immune cells, causing immunological complications of CKD patients more susceptible to infection than normal people. The purpose of this study is to identify the type of aerobic bacteria in the urine of patients with CKD Stage 5 in Inpatient B and C BLU Inpatient Hospital. PROF. R.D Kandou Manado. This study used a prospective descriptive method with a sample of 20 samples and carried out in November 2014 - January 2015. The results of urine culture and biochemical tests showed bacterial growth were identified, namely Escherichia coli, Streptococcus sp., Staphylococcus sp., Coccus Gram-negative, Gram Coccus positive, Seratia Marcesnes, and Bacillus subtilis. From the results of this study, the conclusions on most types of bacteria found were Bacillus subtilis (50%).Keywords: CKD stage 5, aerobic bacteria, urine middle portionAbstrak: Penyakit ginjal kronis (PGK) merupakan penyakit kronis yang dapat menyebabkan beberapa kelainan di dalam sistem pertahanan tubuh yang memungkinkan peningkatan risiko terkena infeksi, salah satunya infeksi saluran kemih. Pada penderita PGK stadium 5, fungsi imunologis terganggu dan infeksi sering terjadi. Kejadian infeksi yang tinggi dijumpai pada penderita uremik. Uremia menekan fungsi sebagian besar sel imun penderita, adanya komplikasi imunologis menyebabkan penderita PGK lebih mudah terkena infeksi dibandingkan orang normal. Tujuan dari penelitian ini adalah untuk mengidentifikasi jenis bakteri aerob pada pemeriksaan urin pasien PGK Stadium 5 di Instalasi Rawat Inap B dan Instalasi Rawat Inap C BLU RSUP. PROF. R.D Kandou Manado. Penelitian ini menggunakan metode deskriptif prospektif dengan sampel sebanyak 20 sampel dan dilaksanakan pada bulan November 2014 – Janurari 2015. Hasil kultur urine dan uji biokimia menujukkan pertumbuhan bakteri yang teridentifikasi yaitu Eschericia coli, Streptococcus sp., Staphylococcus sp., Coccus Gram negatif, Coccus Gram positif, Seratia Marcesnes, dan Bacillus Subtilis. Dari hasil kesimpulan pada penelitian ini didapatkan jenis bakteri yang paling banyak di temukan adalah Bacillus Subtilis (50%).Kata Kunci : PGK stadium 5, bakteri aerob, Uuin porsi tengah

scholarly journals Evaluation of the surgeon’s workplace in elective procedures: a descriptive study in two Brazilian public hospitals

2021 ◽  
Vol 8 (5) ◽  
pp. 151-158
Author(s):  
Francisca Miranda de Araujo ◽  
Raimundo Lopes Diniz ◽  
Ana Hlia dee Lima Sardinha ◽  
Rubenio Barros
Keyword(s):  
Operating Room ◽  
Psychosocial Factors ◽  
Working Conditions ◽  
Organizational Management ◽  
Descriptive Study ◽  
Psychological Problems ◽  
Public Hospitals ◽  
Field Observations

The surgeon is exposed to ergonomic constraints (physical and mental) that may influence on his health from aspects related to physical posture during work to psychological problems arising from the management conditions in the operating room settings. This study evaluates the working conditions during elective surgeries in two Brazilian Public Hospitals. Field observations and a questionnaire were performed. The results indicated dissatisfaction and risks regarding the discomfort/pain, Physical/Environmental, Organizational/Management, and Psychosocial factors.

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