scholarly journals Identification of pathogenic bacteria from infected Thai koi (Anabas testudineus)

2019 ◽  
Vol 5 (1) ◽  
pp. 56-62
Author(s):  
Mousumi Sarker Chhanda ◽  
Imran Parvez ◽  
Nazmi Ara Rumi ◽  
Md Hafiz All Hosen ◽  
Md Rezaul Islam
Keyword(s):  
Experimental Infection ◽  
Pathogenic Bacteria ◽  
Culture Media ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Anabas Testudineus ◽  
Mannitol Salt Agar ◽  
Fresh Fish ◽  
Agar Media ◽  
Staphylococcus Spp

A study was conducted for identification of pathogenic bacteria from Thai koi (Anabas testudineus) and an experimental infection test was run for identifying the actual causative agent of the infection. Due to perform the experiment, the fish sample was collected from different fish farm located in Fulbari, Dinajpur to the Microbiology lab of the HSTU-Dnajpur campus and placed in tray for taking sample from different infected part such as gill, slime, muscle, fin of the fish by using wire loop then these were taking in the nutrient ager medium for observing the culture of bacteria. After then specific culture media, Salmonella-Shigella media, Mannitol salt agar media, Mac-Conkey (MaC) agar media and Eosin Methylene Blue media were used for observing specific bacterial characteristics. Then biochemical tests, Methyl red (MR), Voges-proskaure test, Triple sugar iron test, Indole test were performed for bacterial identification. As a result Salmonella spp., Shigella spp., Klebshiella spp. and Staphylococcus spp. were confirmed. Catalase test and Simon citrate test was also performed. Then Grams staining method was followed for microscopic observation of identified bacteria. Then experimental infection test was performed in Aquaculture lab by setting up 5 aquarium holding fresh fish. The fresh water and identified bacteria were added specifically to the aquarium and it was continued for 15 days for observing infectious symptoms. After 15 days the fish with Salmonella spp. and Staphylococcus spp. were showed infectious symptoms but other did not any change in physical appearance. So it can be said that Staphylococcus spp. and Salmonella spp. are able to show ulcerative symptoms in Thai koi (Anabas testudineus) that is a bacterial infection. Asian J. Med. Biol. Res. March 2019, 5(1): 56-62

scholarly journals Possible Bacteria Pathogens Found in the Internal Surface of Ladies Handbags in Umuahia, Abia State, South-Eastern Nigeria

2020 ◽  
pp. 66-73
Author(s):  
Nwankwo E.O. ◽  
Keyword(s):  
Pathogenic Bacteria ◽  
Culture Media ◽  
Bacterial Pathogens ◽  
Internal Surface ◽  
Health Concern ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Coagulase Negative Staphylococci ◽  
Microbiological Methods

Bacteria including the pathogenic species have been isolated from fomites, these organisms are sometimes multidrug resistant and are of public health concern. It is therefore important to isolate and identify potential bacterial pathogens associated with the internal surface of ladies handbags, in Umuahia, Abia state. One hundred and forty swabbed samples were collected from the ladies hand bags in different groups of individuals which include; Nurses, civil servants, students and market women. Also the handbags from which the samples were collected includes: Leather, Cotton, Nylon and Polyester and velvet handbags. The bags were swabbed with sterile swab sticks and inoculated on different types of culture media and incubated at 37o C for 24 hours. Bacterial isolates were identified using standard microbiological methods including biochemical tests before subjecting isolates to different antimicrobial sensitivity test that was carried out by disc diffusion method. The following bacteria were isolated from the internal surface of the handbags, Coagulase Negative Staphylococci 6(2.6%), Escherichia coli 36(15.7%), Klebsiella spp. 14(6.1%), Staphylococcus aureus 49(21.3%), Bacillus spp. 48(20.9%), Pseudomonas aeruginosa 5(2.2%), Proteus spp. 5(2.2%), streptococcus spp. 31(13.5), Micrococcus spp. 20(8.7%), Salmonella spp. 3(1.3%) and Enterococcus faecalis 13(5.7%). Most of the isolates were sensitive to levofloxacin, gentamicin, norfloxacin, ciprofloxacin and resistant to ampiclox, chloramphenicol and erythromycin. Potentially pathogenic bacteria resistant to multiple antibiotics can be spread by hand contact from ladies handbags. Keywords: Bacterial pathogens, ladies handbags, antibiogram

scholarly journals CHARACTERIZATION OF BACTERIA ASSOCIATED WITH PNEUMONIA IN BLACK BENGAL GOATS

2012 ◽  
Vol 9 (1) ◽  
pp. 67-71 ◽  
Author(s):  
MA Momin ◽  
MA Islam ◽  
MM Khatun ◽  
MM Rahman ◽  
MA Islam
Keyword(s):  
Antimicrobial Agents ◽  
Research Work ◽  
Nutrient Agar ◽  
Blood Agar ◽  
Biochemical Tests ◽  
Highly Sensitive ◽  
Golden Yellow ◽  
Agar Media ◽  
Staphylococcus Spp

The present research work was undertaken for the characterization of the bacterial pathogens responsible for pneumonia in black Bengal goats. Nasal swab samples (n = 50) were collected from the pneumonic black Bengal goats in Mymensingh and Sirajgonj districts. Samples were inoculated onto nutrient agar, eosin methylene blue (EMB) agar, MacConkey agar, and blood agar media for isolation of bacteria. Identification of bacteria was performed by the Gram's staining method, cultural properties and biochemical tests. Antibiotic sensitivity of bacterial isolates was performed against 11 antimicrobial agents. Pasteurella spp were isolated from 25 cases, and Staphylococcus spp from 13 cases. Mixed infection caused by the Pasteurella spp and Staphylococcus spp. were recorded in 12 cases. Pasteurella spp produced whitish, opaque circular and translucent colonies on nutrient agar, smooth, convex, glistening colonies on EMB agar and no hemolysis on blood agar. Staphylococcus spp have shown gray white or golden yellowish colonies on  nutrient agar. Golden yellow colonies without hemolysis or whitish colonies with hemolysis were also produced by Staphylococcus spp. on the blood agar media. Pasteurella spp were indole positive, MR-VP negative and ferment dextrose, sucrose and mannitol with the production of acid. The Staphylococcus spp were positive to MR-VP, coagulase and catalase reactions, negative to indole test and fermented five basic sugars with acid production. Results of cultural and biochemical tests supported that these two isolates belonged to P. multocida and S. aureus. P. multocida were highly sensitive to ciprofloxacin and resistant to penicillin. S. aureus found to be highly sensitive to erythromycin, tetracycline, enrofloxacin, and norfloxacin and less sensitive to amoxicillin. DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11215Bangl. J. Vet. Med. (2011). 9(1): 67-71 

scholarly journals Detection of pathogenic bacteria associated with earphones used by students of Stamford University Bangladesh

2020 ◽  
Vol 10 (1) ◽  
pp. 1-4
Author(s):  
Omor Ahmed Chowdhury ◽  
Md Raihan Ahmed ◽  
Md Raihan Dipu ◽  
Md Aftab Uddin
Keyword(s):  
Pathogenic Bacteria ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Disk Diffusion Method ◽  
Antibiotic Resistant ◽  
Potential Source ◽  
Different Types ◽  
Staphylococcus Spp

The use of earphones has increased in recent times throughout the world especially among the different level of students such as school, college or university who have a higher tendency of sharing these among them. Unlike airline headsets, headphones and stethoscope ear-pieces, ear phones are often shared by multiple users and can be a potential medium for transmission of pathogens, which can give rise to various ear related infections. The objective of this study was to detect the pathogenic bacteria from the ear-phones used by the students of Stamford University Bangladesh. A total of 16 ear-phone swabs were collected by sterile cotton swabs. The swabs were inoculated onto blood agar and incubated aerobically overnight at 37oC. Microscopic observation and standard biochemical tests were performed to confirm the identification of all the bacterial isolates. Six presumptively identified Staphylococcus spp. (38%) were tested against six different types of antibiotics following Kirby-Bauer disk diffusion method. Isolates were found to be 84% resistant against Cotrimoxazole and demonstrated 100% sensitivity to Vancomycin and Ciprorofloxacin. The findings of this study suggest the users to disinfect their respective ear phones and not to exchange them as they may act as a potential source to transfer pathogenic and antibiotic resistant bacteria among the ear phone users. Stamford Journal of Microbiology, Vol.10 (1) 2020: 1-4

scholarly journals Evaluation of sanitary quality of goat milk in dairy industries from the Cariri region, state of Paraíba

2021 ◽  
Vol 10 (12) ◽  
pp. e596101220735
Author(s):  
Iara Nunes de Siqueira ◽  
Aline Antas Cordeiro Cavalcanti ◽  
Joyce Galvão de Souza ◽  
Filipe Jordão Pereira de Medeiros ◽  
João Carlos Taveira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Pathogenic Bacteria ◽  
Goat Milk ◽  
Salmonella Spp ◽  
Pasteurized Milk ◽  
Enterococcus Spp ◽  
Bacillus Spp ◽  
Thermotolerant Coliforms ◽  
Staphylococcus Spp

The sanitary evaluation of equipment and hands is fundamental to investigate the presence of pathogens in the dairy industry. Then, this study aims to evaluate the sanitization of equipment, workers’ hands, raw and pasteurized milk in goat milk dairies in the Cariri region, state of Paraíba.  Collected 32 samples of four dairies represented by letters A, B, C, and D. The followings contents were analyzed: mesophiles, total and thermotolerant coliforms, Escherichia coli, Staphylococcus aureus, Samonella spp. and Listeria monocytogenes in the reception tank, pasteurization tank, packing machine, package, wall, workers’ hand, and each dairy’s raw and pasteurized milk. After isolation, 84 colonies were confirmed by MALDI TOF. The indicator microorganisms presented variations for the workers’ hands, while A and B stayed within the patterns. For the equipment, only dairy B was within limits. They were out of the standard for mesophiles, total coliforms, and thermotolerant regarding raw and pasteurized milk. The microorganisms, the Enterobacteriaceae family presented a higher frequency, with 77.38%, and within this family, Escherichia coli, Klebsiella spp., and Enterobacter spp. were the most prevalent. Gram-positive corresponded to 22.62%, Bacillus spp., Staphylococcus spp., Enterococcus spp., and Macrococcus caseolyticus. Listeria monocytogenes and Salmonella spp. were not isolated. These demonstrate failures in goat milk processing with pathogenic bacteria in several dairy plants, indicating the need to adjust the product’s quality control.

scholarly journals Comparison of the Effect Aqueous Extract and Nano-Sidr Synthetic of Leaves Ziziphus Spina-christi with Silver Nanoparticles the on Biofilm of Local Isolates of Staphylococcus. Spp

2021 ◽  
pp. 178-190
Author(s):  
Furqan Fadhil Najm ◽  
Siham Jasim Alkaabi ◽  
Quraish Abbas Kadhum
Keyword(s):  
Silver Nanoparticles ◽  
The Other ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Ag Nps ◽  
Staphylococcus Lugdunensis ◽  
Mannitol Salt Agar ◽  
Staphylococcus Warneri ◽  
Vitek 2 ◽  
Staphylococcus Spp

Aims: This study aims to investigate the inhibitory susceptibility of extract Sidr leaf (Ziziphus spina-christi) aqueous, silver nanoparticles Ag NPs and Sidr nanoparticle "Nano-sidr" (Ziziphus spina-christi) against local isolates of Staphylococcus.SPP, and their inhibition effect on biofilm formation. Study Design: Initially diagnosed by The use of Mannitol salt agar medium, then 26 isolates were selected from them depending on the resistance to methicilln and Vancomycin that were conducted. Place and Duration of Study: The samples were collected from AL-Najaf AL-Ashraf and Baghdad hospitals. Methodology: These isolates were subjected to a VITEK-2 compact system "ID, AST", to ascertain the genus and type of Staphylococcus bacteria., morphological and biochemical tests were conducted on them to confirm them. Results: The result of the diagnosis showed 10 isolates belonging to Staphylococcus aureus, and a number of them were resistant to as MRSA-VRSA, and 7 were Staphylococcus haemolyticus and they were all resistant and known as MRSA-VRSA, and two isolates of Staphylococcus sciuri bacteria, one of them was resistant to VRSA and the other to MRSA-VRSA , two isolates of Staphylococcus warneri were both resistant to MRSA, two isolates of Staphylococcus lugdunensis were resistant to antibiotics, one of them was VRSA and the other was MRSA-VRSA, and one isolate of Staphylococcus lentus was also resistant to antibiotics MRSA-VRSA, and one isolate of Staphylococcus warneri was resistant to MRSA-VRSA, and one isolate of Staphylococcus lugdunensis was resistant to antibiotics. Conclusion: Isolation of Staphylococcus vitulinus. 11 different isolates were selected from them according to their resistance to antibiotics, and after selecting the most efficient one by examining the inhibitory activity by diffusion method. The results showed the ability of both types of nanoparticles. Plant extracts prevent the formation of biofilms

scholarly journals Microbiological analysis of different categories of food items in Dhaka city, Bangladesh

Food Research ◽  
2020 ◽  
Vol 4 (4) ◽  
pp. 1351-1356
Author(s):  
M.A. Uddin ◽  
T. Jabin ◽  
E.A. Siam ◽  
M.T. Afreen ◽  
S.S. Uddin ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Bacterial Count ◽  
Food Samples ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Wide Range ◽  
Shigella Spp

Pathogenic microorganisms have been so far reported to contaminate a wide range of foods triggering food borne infections or intoxications including the enteric complications, abdominal pain, fever, bloodstream infection etc. The current study was attempted to observe the microbiological quality of some popular foods collected from different places of Dhaka city, Bangladesh. Conventional cultural, microscopic and biochemical tests were followed for the detection and enumeration of bacterial isolates associated with these food samples. The investigation encompassed detection of total viable bacterial count (TVBC) and presumptive identification of other pathogenic bacteria from these samples. Higher counts of TVBC, coliform, Staphylococcus spp. and fungal load were recorded as 1.46×107 CFU/g (yogurt 1), 4.5×106 CFU/g (yogurt 1), 5.6×105 CFU/g (raw meat) and 2.9×103 CFU/g (sea fish), respectively. Fecal coliform was detected only in one out of ten samples. On the contrary Salmonella spp., Vibrio spp., Shigella spp. and Pseudomonas spp. were completely absent. The antibiogram study showed that all the isolates were sensitive against Kanamycin and Azithromycin. Better sensitivity was also observed against Gentamycin and Ciprofloxacin. Varying degree of antibiotic resistance was also detected against Cefixime, Amikacin and Neomycin. Our study emphasizes the need for continuous monitoring of the various categories of food samples for the safety of public health.

scholarly journals Identification of Pathogenic Bacteria from Diseased Thai Pangas Pangasius hypophthalmus with Their Sensitivity to Antibiotics

2020 ◽  
pp. 7-21
Author(s):  
Moslima Parven ◽  
M. M. Mahbub Alam ◽  
Sarker Md. Ibrahim Khalil ◽  
Aishi Hamom ◽  
Osman Goni ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Antibiotic Sensitivity ◽  
Pseudomonas Sp ◽  
Biochemical Tests ◽  
Important Species ◽  
High Productivity ◽  
Fresh Fish ◽  
Sugar Fermentation ◽  
Antibiotic Sensitivity Test ◽  
Diseased Fish

Thai Pangas (Pangasiaus hypophthalmus) has been emerging as an economically very important species due their faster growth, year round production and high productivity in South-East Asian aquaculture. It has good market value as a food fish due to its good taste and deliciousness with high protein, minerals and fat content. It is also popular as a game fish. Aims: The present study was conducted to identify bacterial pathogens in diseased P. hypophthalmus and evaluate their sensitivity to antibiotics. Methodology: To identify the causativeagent of diseased fish pure culture of bacteria using slant and streak plate techniques and different biochemical tests such as Gram’s Staining, Motility Test, Sugar Fermentation Test, MR-VP test, Indole Test etc. were performed. To assess the sensitivity of the isolated bacteria to antibiotics five antibiotics disks i.e. Ciprofloxacin (5μg), Azithromycin (15μg), Ampicillin/Sulbactam (20μg), Tetracycline (30μg) and Erythromycin (15μg) were used. Results: Three pathogenic bacteria i.e. Aeromonas hydrophila, Edwardsiella ictaluri and Pseudomonas sp. were identified in the studied diseased fish. Only Pseudomonas sp. were identified from Fresh fish. E. ictaluri was found only in diseased pangas which was the causative agent for thedisease, Bacillary Necrosis. The results of the antibiotic sensitivity test showed multi-resistances of the identified bacteria to the tested antibiotics. The identified bacteria were 100% sensitive to Ciprofloxacin (5μg), intermediate to Azithromycin (15μg) and Tetracycline (30μg), but resistant to Erythromycin (15μg) and Ampicilin/Sulbactam (20μg). Conclusion: Ciprofloxacin (5µg) could be used to control bacillary necrosis disease in Thai pangas. The results of this study will be helpful to the fish farmers for the management of bacterial diseases in fish.

scholarly journals Influence of tannery wastes and the surrounding environment of tannery industries on microbial growth and proliferation

2017 ◽  
Vol 7 (1) ◽  
pp. 14-18
Author(s):  
Nayan Chandra Das ◽  
Farzana Hossaini ◽  
Saurab Kishore Munshi
Keyword(s):  
Pathogenic Bacteria ◽  
Multidrug Resistant ◽  
Salmonella Spp ◽  
Tannery Waste ◽  
Surrounding Environment ◽  
Bacillus Spp ◽  
Tannery Wastes ◽  
Bacteria And Fungi ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

The present study was carried out to assess the degree of microbiological proliferation in tannery wastes and the surrounding environment of the tannery industries. In this regard, a total of 8 tannery waste (n=4) and environmental (n=4) samples were tested. All the samples contained a huge load of bacteria and fungi in an average of 108 cfu/g or ml. An extended numbers of pathogenic bacteria were recovered. Among the pathogenic bacteria, Staphylococcus spp. was predominant. Most of the samples exhibited the presence of Pseudomonas spp. Salmonella spp. and fecal coliform were found each in one sample. Bacillus spp., Escherichia coli, Klebsiella spp. and Vibrio spp. were found in few samples. The average load of the pathogens was 104 cfu/g or ml. All the pathogenic isolates were found to be multidrug resistant. Higher resistance was found against penicillin and streptomycin. Tannery waste after lather treatment sample showed antibacterial activity against all the pathogens tested. Overall, presence of pathogenic microorganisms with multidrug resistance traits may pose serious public health threats. Stamford Journal of Microbiology, Vol.7(1) 2017: 14-18

scholarly journals Identificación bacteriana en teléfonos celulares de estudiantes de medicina que acuden o no, a un Hospital General en Mérida, Yucatán, México

2019 ◽  
pp. 21-25
Author(s):  
Angel D. CAAMAL-LEY ◽  
Miguel A. PUC-FRANCO ◽  
Mario R HEREDIA-NAVARRETE ◽  
David LINDO-PEREZ ◽  
Alberto VARGAS-GONZALEZ
Keyword(s):  
Information Exchange ◽  
Pathogenic Bacteria ◽  
Culture Media ◽  
Cell Phones ◽  
Bacterial Species ◽  
Isotonic Saline ◽  
Medical Personnel ◽  
Biochemical Tests ◽  
Gram Staining ◽  
First Year Medical Students

The cell phone is an important tool for communication, consultation and information exchange; Studies carried out in hospitals have shown that cell phones of medical personnel are contaminated by nosocomial pathogens. However, the bacterial species that the cell phones house and their potential risk have not been determined. We analized the cell phones of 30 first-year medical students who do not visit the hospital in their studies vs. 30 students who perform their clerkship practices in hospitals, to determine the differences in bacterial loads. Samples were taken by sterile swabs moistened with isotonic saline. McConkey and salt-mannitol agar were used as culture media. The biochemical tests used for identification of enterobacteria were citrate, MIO, LIA, urea and TSI. Degradation tests of mannitol, coagulase, catalase and oxidase were used for the identification of staphylococci. Likewise, smears and Gram staining of the isolated colonies were performed. Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli and Pseudomona sp. were mainly found. Differences in number and bacterial species were found in the cell phones of each study group, representing a reservoir of potentially pathogenic bacteria for humans.

scholarly journals Prevalence and Antibiogram Profiles of Salmonella Isolated from Pigeons in Mymensingh, Bangladesh

2013 ◽  
Vol 1 (2) ◽  
pp. 54-57 ◽  
Author(s):  
Md Sahadat Hosain ◽  
Md Ariful Islam ◽  
Mst Minara Khatun ◽  
Rubel Kanti Dey
Keyword(s):  
Culture Media ◽  
Health Hazard ◽  
Antibiotic Sensitivity ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Live Bird Markets ◽  
Sensitivity Tests ◽  
Culture Positive ◽  
Live Bird

This study was undertaken to determine the prevalence of Salmonella spp. in apparently healthy pigeons at the live bird markets, farms and villages in the Mymensingh district of Bangladesh. Cloacal swabs (n = 36), foot pads (n = 36) and feces (n = 40) of pigeons were collected aseptically and inoculated onto various culture media for isolation of Salmonella. Identification of Salmonella from culture positive samples was performed by cultural characteristics, Gram’s staining and biochemical tests. The prevalence of Salmonella in cloacal swabs, foot pads and feces was 22.22%, 58.33% and 27.50%, respectively. The overall prevalence of Salmonella in pigeons was 35.71%. Pigeons at live bird markets, farms and villages showed a 40.48%, 20% and 30% prevalence of Salmonella, respectively. Antibiotic sensitivity tests of the Salmonella was performed by the disc diffusion method against 10 randomly used antibiotics. The highest rate of resistance was found with amoxicillin (90%) followed by ampicillin (80%), erythromycin (80%) and tetracycline (60%). The highest rate of sensitivity was recorded to ciprofloxacin (80%) followed by sulphamethoxazole (70%), chloramphenicol (60%), kanamycin (60%), gentamicin (60%) and nalidixic acid (60%). Data from this study suggest that pigeons carry multi-drug resistant Salmonella which can be transferred to humans through direct contact or the food chain and could cause a potential public health hazard. DOI: http://dx.doi.org/10.3329/mh.v1i2.14090 Microbes and Health, 2012 1(2): 54-57

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