scholarly journals Detection of Entamoeba histolytica, Giardia lamblia and Cryptospodium sp. Infection among diarrheal patients

2015 ◽  
Vol 43 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Hamida Khanum ◽  
Rukhshana Kadir ◽  
Tuhinur Arju ◽  
Tahmina Ahmed ◽  
Mt Tahmina Karim ◽  
...  

Tri-combo ELISA tests were carried out to determine the prevalence of protozoan parasites (Entamoeba histolytica, Giardia lamblia and Cryptosporidium spp) in 1712 stool samples of diarhoeal patients. Among the samples tested, 118 (6.89%) were found positive. The age group 6-14 years were recorded to be more susceptible (14.81%) and prevalence gradually decreased as the age group increased. Individual Antigen Detection Tests were done on the positive samples and 1 sample was noted to carry E. histolytica (0.06%), 92 (5.37%) samples with G. lamblia and 22 (1.29%) samples with Cryptosporidium spp. Maximum prevalence was recorded in summer (8.47%) and rainy seasons (8.14%) and highest infections (10.46%) were found in the month of September The prevalence of G. lamblia was higher in male (5.37%) whereas, the prevalence of Cryptosporidium spp. was higher in female (1.64%).Bangladesh J. Zool. 43(1): 1-7, 2015

2012 ◽  
Vol 36 (1) ◽  
pp. 1-5
Author(s):  
Amjed Qays Ibrahim

In this study we collect 1520 stool samples during the period from September to December 2010 from children whom their ages between 1 month - 12 years. The results showed that the total infection of Entamoeba histolytica was 9.80% , and Giardia lamblia was 1.77%. And the male ratio that infected with Entamoeba histolytica was 9.83% , while the female ratio was 9.74%; and the male infected with Giardia lamblia was 1.51% , while the female ratio was 2.18%. The result showed that the high average of infection with Entamoeba histolytica and Giardia lamblia in age group from 1 month to 2 years. And there is no significance difference between gender and infectivity rate of Entamoeba histolytica and Giardia lamblia under P≤0.05. Also it showed that there were significant relation between Age group and infectivity rate of Entamoeba histolytica and Giardia lamblia.


2013 ◽  
Vol 2013 ◽  
pp. 1-6
Author(s):  
Vani Chandrashekar

The aim of this study was to identify common stool parasites in patients attending a tertiary care centre in South India. We evaluated 2355 stool samples and parasites were detected in 7.9% of samples. 41.1% of our patients were in the 45–58-year age group. Protozoal infections were the commonest seen in 7.8% of samples. Entamoeba histolytica was the commonest protozoa (4.6%) followed by Entamoeba coli (1.2%) and Giardia (0.8%). Entamoeba histolytica and Entamoeba coli were together seen in 0.63%, and they were the commonest organisms seen in samples with multiple-organism infection. Both were equally detected in diarrheal samples.


2017 ◽  
Vol 117 (2) ◽  
pp. 447-451 ◽  
Author(s):  
Marijo Parčina ◽  
Ingrid Reiter-Owona ◽  
Frank P. Mockenhaupt ◽  
Valerija Vojvoda ◽  
Jean Bosco Gahutu ◽  
...  

2007 ◽  
Vol 40 (3) ◽  
pp. 346-348 ◽  
Author(s):  
Filipe Anibal Carvalho-Costa ◽  
Alessandra Queiroga Gonçalves ◽  
Sandra Laranjeira Lassance ◽  
Carla Pontes de Albuquerque ◽  
José Paulo Gagliardi Leite ◽  
...  

The objective of the present study was to estimate the frequency of infection by Cryptosporidium spp and other intestinal parasites in dehydrated children with gastroenteritis who were admitted to a pediatric hospital. Stool examinations from 218 children were performed. Cryptosporidium spp was identified in eighteen out of 193 stool samples (9.3%) subjected to safranin-methylene blue staining. Giardia lamblia was detected in ten out of 213 (4.7%) samples examined via the direct or Ritchie methods. Other parasites identified were Ascaris lumbricoides (4.2%), Blastocystis hominis (1.4%), Entamoeba coli (0.9%), Entamoeba histolytica/Entamoeba dispar (0.5%), Endolimax nana (0.5%), Trichuris trichiura (0.5%) and Enterobius vermicularis (0.5%).


Author(s):  
Firas Fahad Habeeb ◽  
Ali Mohammed Abed

The current study was conducted to detect the infection rate of the Entamoeba histolytica and Giardia lamblia parasite and to determine the relationship between them and Helicobacter pylori bacteria for patients attending Shirqat General Hospital in Salah al-Din Governorate during the period from October 2020 to March 2021. The number of examined samples reached 409, as they were examined by direct smear and Concentration methods using an optical microscope. The results of the study showed that the total infection rate was 46 (11.24%) positive samples and 363 negative samples with a rate of (88.7%), the Entamoeba histolytica was dominant with rate 67.38%, followed by Giardia lamblia with rate 32.61%, and the infection rates for males were higher than for females. Where the infection rate of males reached 14.29% (34 out of 238) and 7.02% of females (12 out of 171), respectively. The infection rates varied between age groups, as the highest rate of infection was in the age group ≥ 10-1 years, which amounted to 32.6%, and the lowest rate of infection was in the age group 60-51 years, with a rate of 2.17%. The highest infection rate was in October, which amounted to 14.89%, while the lowest infection rate was in December, with a rate of 7.35%. All positive samples were examined for microscopic examination by rapid antigen test to detect H.pylori bacteria involved in infection with Entamoeba histolytica or Giardia lamblia, and the results showed that 13 (28.26) samples were positive and 33 (71.73%) were negative samples, and the Co-infection infection rate was between the Entamoeba histolytica and H.pylori 9 (29.03%) were higher than that of Giardia lamblia and H.pylori 4 (26.66%).The results of the statistical analysis showed that there were significant differences between the sex of patients infected with these parasites and the rates of infection during the months of the study and the age groups of infected patients.


Author(s):  
JA’AFAR S ADAM ◽  
MUSA G YAHAYA ◽  
FAROUK S NAS ◽  
LURWAN MU’AZU ◽  
MUHAMMAD ALI

Background: Diarrhea is a major contributor to childhood mortality and morbidity in the developing world. Objective: The study was aimed to determine the prevalence of Entamoeba histolytica among children diagnosed with acute diarrhea in Kura General Hospital Kano, from February to August 2017. Methodology: A total of 236 stools samples were collected from children within the age group of 0 – 5 years diagnosed with acute diarrhea attending the Hospital. The stool samples were examined for Entamoeba Histolytica cysts and trophozoites using direct wet preparation and Formol Ether concentration techniques. Results: The result showed that 23 samples which accounted for 9.75% of the samples were found to be positive for the parasite. Highest incidence of the parasite was found among children within the age category of 4 - 5 years. The results also indicated that males (56.5%) were more infected than females (43.5%). However, there is no statistical difference in the rate of infection among the sex and age group of the patients at p< 0.05. Conclusion: In conclusion, maintenance of personal hygiene and improved sanitation of the environment will indeed prevent contamination of food and water sources.


2018 ◽  
Vol 45 (2) ◽  
pp. 159-170
Author(s):  
Rimi Farhana Zaman ◽  
Hamida Khanum ◽  
Shakila Nargis ◽  
Ponkoj Kumar Das

The purpose of the present study was to compare results of saline, iodine, and KOH wet mount preparations executed on each of 1116 stool samples from patients attending ICDDR,B clinical pathology laboratory was compared. Ascaris lumbricoides was most prevalent helminth (3.67%) found in KOH wet mount preparation. The protozoan parasites (9.59%) were more prevalent than helmiths (8.24%) and Giardia sp (4.03%) shows the higher infection found by KOH wet mount preparation. Females are more infected by parasites than males found in observations by all the three methods (saline, iodine and KOH wet mount preparation). It was also observed that the young (1- 10 years) were more infected than older age group (above 51). In seasonal variation, parasitic infestation was higher during May (27%) and the prevalence was lower in December (10%). KOH was found to be effective in destroying the faecal debris, faecal mass and other artifacts without affecting many of the parasitic ova, cysts and larvae in the stool smears. KOH for wet mount smear in examining the stool samples for detection of intestinal helminths may be routinely used which is simple, cost-effective and could be afforded in resource poor setting.Bangladesh J. Zool. 45(2): 159-170, 2017


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