scholarly journals Detection of Cryptosporidium spp and other intestinal parasites in children with acute diarrhea and severe dehydration in Rio de Janeiro

2007 ◽  
Vol 40 (3) ◽  
pp. 346-348 ◽  
Author(s):  
Filipe Anibal Carvalho-Costa ◽  
Alessandra Queiroga Gonçalves ◽  
Sandra Laranjeira Lassance ◽  
Carla Pontes de Albuquerque ◽  
José Paulo Gagliardi Leite ◽  
...  
Keyword(s):  
Giardia Lamblia ◽  
Intestinal Parasites ◽  
Blue Staining ◽  
Pediatric Hospital ◽  
Severe Dehydration ◽  
Enterobius Vermicularis ◽  
Blastocystis Hominis ◽  
Entamoeba Dispar ◽  
Cryptosporidium Spp ◽  
Stool Samples

The objective of the present study was to estimate the frequency of infection by Cryptosporidium spp and other intestinal parasites in dehydrated children with gastroenteritis who were admitted to a pediatric hospital. Stool examinations from 218 children were performed. Cryptosporidium spp was identified in eighteen out of 193 stool samples (9.3%) subjected to safranin-methylene blue staining. Giardia lamblia was detected in ten out of 213 (4.7%) samples examined via the direct or Ritchie methods. Other parasites identified were Ascaris lumbricoides (4.2%), Blastocystis hominis (1.4%), Entamoeba coli (0.9%), Entamoeba histolytica/Entamoeba dispar (0.5%), Endolimax nana (0.5%), Trichuris trichiura (0.5%) and Enterobius vermicularis (0.5%).

scholarly journals The Incidence Of Intestinal Parasites Among Children In Hivi Pediatric Hospital, Duhok, Iraq

2019 ◽  
Vol 7 (1) ◽  
pp. 1-4
Author(s):  
Jehan N. Hussein ◽  
Azad A. Meerkhan
Keyword(s):  
Infection Rate ◽  
Giardia Lamblia ◽  
Intestinal Parasites ◽  
Gastrointestinal Parasites ◽  
Age Group ◽  
Mixed Feeding ◽  
Pediatric Hospital ◽  
Enterobius Vermicularis ◽  
Urban Residence ◽  
Cryptosporidium Spp

This study has conducted in Hivi Pediatric Hospital, Duhok Province, Iraq, to estimate the gastrointestinal parasites infection among patients contacted this hospital. Overall patients, 22.27% (261/1,172) were diagnosed as infected by parasites; more of them were from summer season with the infection rate of 24.83% (216/870) versus 14.90% (45/302) in autumn, rural residence had a greater rate of infection 65.90% (172/261) as compared with urban residence 34.09 (89/261), males were more infected than females (63.98% versus 36.02% respectively), the age group of three years and less had the highest rate of infection, while the age group of more than 9 years had the lowest rate (57.85%, 4.98% respectively). Concerning the children feeding way, the artificially feeding had the highest rate then mixed feeding and Breastfeeding (58.16%, 25.53% and 16.31% respectively). As general, the infection with protozoa (84.67) was higher than helminthes (18.01%) with some cases of infection by both of protozoa and helminthes (4.98). Regarding each parasite separately, Entamoeba histolytica had the highest infection rate then Giardia lamblia., Enterobius vermicularis, Cryptosporidium Spp., Entamoeba coliandBlastocystis hominis (38.70%, 25.67%, 18.01, 9.20%, 6.51 and 4.60 respectively).

scholarly journals Blastocystis hominis and other intestinal parasites in a community of Pitanga City, Paraná State, Brazil

2005 ◽  
Vol 47 (4) ◽  
pp. 213-217 ◽  
Author(s):  
Solange Aparecida Nascimento ◽  
Maria da Luz Ribeiro Moitinho
Keyword(s):  
Giardia Lamblia ◽  
High Frequency ◽  
Intestinal Parasites ◽  
Blastocystis Hominis ◽  
Laboratory Techniques ◽  
Wet Mount ◽  
Routine Basis ◽  
Stool Samples ◽  
Zinc Sulphate Solution ◽  
Prevalent Species

The objective was to estimate the prevalence of Blastocystis hominis, to evaluate the effectiveness of different techniques for its diagnosis as well as to estimate the prevalence of other intestinal parasites in the community of Campo Verde, a district of Pitanga. The work was carried out from August to October 2004. Samples of feces from children and adults were collected and submitted to the techniques of direct wet mount, flotation in zinc sulphate solution, tube sedimentation, sedimentation in formalin-ether and staining by Kinyoun and iron hematoxylin methods. From 181 studied individuals, 128 (70.7%) showed protozoa and/or helminths in stool samples. The most prevalent species were Endolimax nana (33.7%); B. hominis (26.5%); Giardia lamblia (18.2%); Entamoeba coli (17.1%); Ascaris lumbricoides (16.6%); Iodamoeba bütschlii (9.4%); and ancylostomatidae (7.7%). B. hominis was only identified by the techniques of direct wet mount, sedimentation in formalin-ether and staining by iron hematoxylin, though the latter was less sensitive than the other methods. The high frequency of B. hominis demonstrated by this study indicates the need to include laboratory techniques that enable identification of the parasite on a routine basis.

scholarly journals Prevalence of Human Intestinal Parasites in Tabriz City of Iran During 2018 and the Importance of These Parasites in Public Health

2020 ◽  
Vol 1 (4) ◽  
pp. 101-104
Author(s):  
Yagoob Garedaghi ◽  
Hamidreza Hassanzadeh Khanmiri
Keyword(s):  
Public Health ◽  
Entamoeba Histolytica ◽  
Giardia Lamblia ◽  
Intestinal Parasites ◽  
World Health ◽  
Parasitic Infections ◽  
Enterobius Vermicularis ◽  
Blastocystis Hominis ◽  
The World ◽  
Tabriz City

Introduction: Intestinal parasitic infections are among the main problems in developing countries. It is estimated that over 4.5 billion people in the world have been infected with a variety of intestinal parasites. Numerous studies conducted in different parts of Iran showed the spread of intestinal parasite infections in different urban and rural areas. However, the prevalence of these infections significantly reduced in recent years due to improvements in public health. Methods: In this cross-sectional study conducted in a hospital in Tabriz city of Iran, 700 stool samples were collected during one year and studied using formalin-ether concentration method and Lugol’s iodine staining. Results: In this study, six types of parasites including Giardia lamblia, Entamoeba histolytica, Entamoeba coli, Chilomastix mesnili, Blastocystis hominis, and Enterobius vermicularis were detected in 700 fecal samples. A total of 112 (16%) samples were infected, among which 43 (40%) were infected with Giardia lamblia, 35 (32%) with Entamoeba histolytica, 20 (18%) with Enterobius vermicularis, 10 (9%) with Entamoeba coli, 3 (0.026%) with Blastocystis hominis, and 1 (0.008%) with Chilomastix mesnili. Conclusion: In this study, the highest prevalence of infection belonged to Giardia lamblia (40%), which is consistent with the statistics published by the World Health Organization. According to the findings of this study, it can be concluded that despite the increase in the provision of health services in cities and villages, the prevalence of intestinal parasites, especially Giardia lamblia, is one of the major problems in the region. Therefore, there is a need for proper and coherent health planning in the region and accurate and up-to-date epidemiological information.

scholarly journals Evaluation of the effectiveness of sterilization materials added to drinking water in the elimination of the Larval stages of parasites in Al-Najaf Al-Ashraf Governorate

2019 ◽  
Vol 10 (4) ◽  
pp. 3665-3668
Author(s):  
Sahira Ayed A. Al-Musawi
Keyword(s):  
Drinking Water ◽  
River Water ◽  
Entamoeba Histolytica ◽  
Water Purification ◽  
Giardia Lamblia ◽  
Intestinal Parasites ◽  
Tap Water ◽  
Enterobius Vermicularis ◽  
Larval Stages ◽  
Ancylostoma Duodenale

The study was conducted to investigate the cysts and eggs of intestinal parasites in drinking water in four models of drinking water for water purification projects in Al-Najaf Al-Ashraf Governorate for the period from 1/10/2017 to 1/10/2018. The results of the examination of river water in four districts in Al-Najaf Al-Ashraf governorate showed the presence of five species of parasites are cysts of  Entamoeba histolytica by 39%, and the eggs of the  Ascaris worm by 12%,  worm Ancylostoma duodenale  by 4%,  Giardia lamblia cysts 40%, and the eggs of  Enterobius vermicularis by 18%. The results of the tap water examination showed that there were five types of intestinal parasites, but at a lower rate than in river water,  Entamoeba histolytica cysts by 16%, eggs of Ascaris by 7%, Ancylostoma duodenale eggs by 2%, Giardia lamblia cysts by 29%, and Enterobius vermicularis by 5% overall.

scholarly journals A five year retrospective analysis of common intestinal parasites at Poly Health Center, Gondar, Northwest Ethiopia

2019 ◽  
Author(s):  
Meseret Ayelgn ◽  
Ligabaw Worku ◽  
Getachew Ferede ◽  
Yitayih Wondimeneh
Keyword(s):  
Health Center ◽  
Giardia Lamblia ◽  
Intestinal Parasite ◽  
Intestinal Parasites ◽  
Age Groups ◽  
Northwest Ethiopia ◽  
P Value ◽  
Wet Mount ◽  
Parasite Infections ◽  
Stool Samples

Abstract Objective: Intestinal parasites are present throughout the world in varying degrees of prevalence due to many factors. The aim of this study was to determine the five-year trend prevalence of intestinal prevalence among patients who had been suspected for intestinal parasite infections. A retrospective study was conducted from 2009 to 2013 at Poly Health Center Gondar, Northwest Ethiopia. Samples were examined using direct saline wet mount methods. Statistical analysis was done using SPSS version 20 software and a P-value of < 0.05 was considered statistically significant. The results were presented in tables and graphs. Results: During the study period, a total of 13,329 stool samples were requested for intestinal parasite diagnose and 5510 (41.3%) laboratory-confirmed cases were reported with a fluctuating trend. Ten different parasites were reported in each year with Entamoeba histolytica/dispar (16.8%) being the predominant parasite followed by Giardia lamblia (11.4%) and Ascaris lumbricoides (6.7%). Both males (49%) and females (51%) were equally affected (P=0.14). The intestinal parasite was reported in all age groups in the area but the highest and the lowest prevalence were reported in age groups of 20-29 years and 40-49 years, respectively (26.5% vs 6.4%) (P < 0.001).

scholarly journals Evaluation of Intestinal Protozoan Morphology in Human Fecal Specimens Preserved in EcoFix: Comparison of Wheatley’s Trichrome Stain and EcoStain

1998 ◽  
Vol 36 (7) ◽  
pp. 1974-1976 ◽  
Author(s):  
Lynne S. Garcia ◽  
Robyn Y. Shimizu
Keyword(s):  
Polyvinyl Alcohol ◽  
Giardia Lamblia ◽  
Blastocystis Hominis ◽  
Intestinal Protozoan ◽  
Entamoeba Dispar ◽  
Intestinal Protozoa ◽  
Mercury Compounds ◽  
Dientamoeba Fragilis ◽  
Color Differences

As a result of disposal problems related to the use of mercury compounds, many laboratories have switched from mercuric chloride-based Schaudinn’s and polyvinyl alcohol (PVA) stool preservatives to other, non-mercury-based preservatives. A comparison of organism recoveries and morphologies of the intestinal protozoa was undertaken with PVA containing the EcoFix zinc-based Schaudinn’s preservative (Meridian Diagnostics, Inc.); both Wheatley’s modification of Gomori’s trichrome stain (WT) and EcoStain (ES) were used to stain 51 human fecal specimens. Morphology, clarity of nuclear and cytoplasmic detail, overall color differences, and the ease or difficulty in detecting intestinal protozoa in fecal debris were assessed for the two permanent stained smears. Overall, organism morphology of the intestinal protozoa stained with WT and that of protozoa stained with ES were not equal in nuclear and cytoplasmic detail or range of color. However, the same organisms were identified in stained fecal smears with either WT or ES, with the exception of situations in which organism numbers were characterized as rare. Included were 67 protozoan challenges (number of organisms): Entamoeba histolytica-Entamoeba dispar (5), Entamoeba coli (9),Entamoeba hartmanni (6), Endolimax nana (12),Iodamoeba bütschlii (8), Blastocystis hominis (19), Giardia lamblia (6), Dientamoeba fragilis (2), yeast (2), and leukocytes (2). Five specimens were negative for parasites but contained fecal debris that was compared for morphologic detail and color range. The ES produces a more gray-green monotone with very little pink or red tone; contrast among the various colors is less than that seen with WT. Stain intensity for all organisms was acceptable, and there were no problems with stain deposition. The quality of the protozoan morphology with ES was often comparable to that with WT (36 of 67 [53.7%]) and, in some cases, better (24 of 67 [35.8%]). Organisms on the WT-stained smear exhibited better morphology in a few instances (4 of 67 [6%]), and in three instances, there were discrepant organism numbers.

scholarly journals Intestinal parasites in school food handlers in the city of Uberlândia, Minas Gerais, Brazil

1995 ◽  
Vol 37 (3) ◽  
pp. 191-196 ◽  
Author(s):  
Julia Maria Costa-Cruz ◽  
Margareth Leitão Gennari Cardoso ◽  
Daldy Endo Marques
Keyword(s):  
Giardia Lamblia ◽  
Intestinal Parasites ◽  
Specific Treatment ◽  
Strongyloides Stercoralis ◽  
Minas Gerais ◽  
Food Handlers ◽  
School Food ◽  
Public Elementary Schools ◽  
Stool Samples ◽  
The City

In order to verify the presence of intestinal parasites in food handlers, stool samples were collected from 104 cooks and their helpers that were working in food preparation in 20 public elementary schools, in various areas of the city of Uberlândia, Minas Gerais, Brazil. The samples were collected during the months of November and December, 1988, in plastic flasks containing a 10% formaldehyde solution and processed by the Hoffmann, Pons & Janer method. The sediment was examined using triplicate slides. All individuals were females aged between 24 to 69 years. Intestinal parasites were found in 85.0% of the studied schools and 47.1% of the studied food handlers (cooks and helpers) were found to be positive. Among the 49 infected food handlers, 32 (65.3%) carried a single parasite and 17 (34.7%) carried two parasites. The following intestinal parasites were found: Giardia lamblia (21.1%), Entamoeba coli (21.1%), hookworms (9.6%), Ascaris lumbricoides (5.8%), Entamoeba histolytica (2.9%), Hymenolepis nana (1.9%), Strongyloides stercoralis (1.0%). These data emphasize the need for a rigid semi-annual control in all school food handlers, including diagnosis, specific treatment and orientation about the mechanisms of transmission of the intestinal parasites.

scholarly journals Intestinal parasitoses and environmental factors in a rural population of Argentina, 2002-2003

2007 ◽  
Vol 49 (4) ◽  
pp. 251-255 ◽  
Author(s):  
Juan A. Basualdo ◽  
María A. Córdoba ◽  
María M. de Luca ◽  
María L. Ciarmela ◽  
Betina C. Pezzani ◽  
...  
Keyword(s):  
Water Samples ◽  
Giardia Lamblia ◽  
Environmental Variables ◽  
Intestinal Parasites ◽  
Soil Samples ◽  
Water Pump ◽  
Blastocystis Hominis ◽  
Degree Of Association ◽  
Sanitary Conditions ◽  
Soil And Water

We evaluate the prevalence of intestinal parasites in 504 people and the degree of association between environmental variables and parasites found in population, soil and water in a rural area of Argentina during 2002-2003. A structured survey was used to evaluate the environmental variables and fecal-human, soil and water samples were analyzed. The prevalence of parasites was 45.4%. Most prevalent protozoa were Blastocystis hominis (27.2%) and Giardia lamblia (6.9%), while the most prevalent helminth was Ascaris lumbricoides (3.8%). The analyzed environmental variables showing association (p < 0.05) with presence of parasites in population were: cardboard-tin or wooden house, dirt floor, home or communal water pump, faucet outside the house or public faucet and cesspool or latrine. Parasite forms were found in 82.3% of the soil samples and in 84.2% of the water samples. In both samples we found parasites that were also found in people. In this study we have found deficient sanitary conditions associated with presence of parasites in population and we have evidenced that contaminated soil and water were the source of these parasites.

scholarly journals Parasitic Contamination of Commonly Consumed Fresh Leafy Vegetables in Benha, Egypt

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Maysa Ahmad Eraky ◽  
Samia Mostafa Rashed ◽  
Mona El-Sayed Nasr ◽  
Azza Mohammed Salah El-Hamshary ◽  
Amera Salah El-Ghannam
Keyword(s):  
Seasonal Variation ◽  
High Risk ◽  
Giardia Lamblia ◽  
Intestinal Parasites ◽  
Parasitic Infection ◽  
Hymenolepis Diminuta ◽  
Leafy Vegetables ◽  
Enterobius Vermicularis ◽  
Hymenolepis Nana ◽  
Significant Seasonal Variation

This study evaluated the degree of parasitic contamination of vegetables which are commercialized and consumed fresh in Benha, Egypt. It included 530 vegetables: lettuce, watercress, parsley, green onion, and leek. Vegetables were collected randomly from markets within Benha. Samples were washed in saline, and the resulting washing solution was filtered and centrifuged to concentrate the parasitic stages. Sediments and supernatants were examined by iodine and modified Ziehl-Neelsen stained smears. Intestinal parasites were detected in 157/530 (29.6%) samples.Giardia lambliacysts were the most prevalent parasite (8.8%) followed byEntamoebaspp. cysts (6.8%),Enterobius vermiculariseggs (4.9%), various helminth larvae (3.6%),Hymenolepis nanaeggs (2.8%),Hymenolepis diminutaeggs (2.1%), andAscaris lumbricoideseggs (0.6%). The highest contaminated vegetable was lettuce (45.5%) followed by watercress (41.3%), parsley (34.3%), green onion (16.5%), and leek (10.7%). These results indicate a significant seasonal variation (P<0.05), with highest prevalence in summer (49%) and the lowest in winter (10.8%). These findings provide evidence for the high risk of acquiring parasitic infection from the consumption of raw vegetables in Benha, Egypt. Effective measures are necessary to reduce parasitic contamination of vegetables.

scholarly journals Prevalence of intestinal parasites in a population in south of Tehran, Iran

2008 ◽  
Vol 50 (3) ◽  
pp. 145-149 ◽  
Author(s):  
Abolfath Shojaei Arani ◽  
Reza Alaghehbandan ◽  
Lame Akhlaghi ◽  
Maryam Shahi ◽  
Abdolaziz Rastegar Lari
Keyword(s):  
Community Involvement ◽  
Intestinal Parasites ◽  
Cross Sectional Study ◽  
Parasitic Infections ◽  
Supporting Evidence ◽  
Blastocystis Hominis ◽  
Cross Sectional ◽  
Intestinal Parasitic Infections ◽  
Public Health Challenge ◽  
Stool Samples

The aim of this study was to describe epidemiologic characteristics of intestinal parasites in a population in south of Tehran, Iran. A retrospective cross-sectional study of patients with suspicious intestinal parasitic infections referred to the Zakaria Razi Laboratory in Shahre-Ray, southern Tehran, Iran, was conducted from April 21, 2004 to October 20, 2005. All stool samples were examined and socio-demographic informations were retrieved. Of 4,371 referred patients, 466 (239 males and 227 females) were laboratory diagnosed with intestinal parasites, with a period prevalence of 10.7%. Blastocystis hominis (B. hominis) and Giardia lamblia (G. lamblia) were the most frequent intestinal parasites. More than half of patients aged ³ 18 years had a low level of educational attainment (e.g. illiterate, primary school, high school) (170/331, 54.1%). Further, majority of patients were homemakers (42.3%, 140/331) or workers (28.1%, 93/331) employed in various business settings such as food industry and construction. Findings of this study showed that intestinal parasitic infections are still a major public health challenge in Iran that needs to be addressed. We believe that public education, improving sanitation conditions of underdeveloped areas/communities, community involvement, and supporting evidence-based practice/programs are the major keys to success in preventing the spread of intestinal parasitic infections in Iran.

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