scholarly journals Synthesis and Cytotoxicity Study of Some Acetylisatins and Diazobiphenyls

1970 ◽  
Vol 9 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Mohammad Mamun Hossain ◽  
Sakanta Kumar Shaha ◽  
Foysal Aziz ◽  
Rahat Khan ◽  
Md Mahabub Hossain
Keyword(s):  
Schiff Base ◽  
Key Words ◽  
Brine Shrimp ◽  
Heterocyclic Compounds ◽  
Cytotoxic Effect ◽  
Cyclization Reaction ◽  
Ring Cleavage ◽  
Anticancer Activities ◽  
Brine Shrimp Lethality ◽  
Anti Inflammatory

Nitrogenous heterocyclic compounds, oxindoles especially isatins and related heterocyles have excellent antifungal, anti-inflammatory, antimicrobial and anticancer activities. Oxindoles keto lactams (1-3) were synthesized according to modified Sandmayer method and their N-acetylated compounds (4-6) gave diazo-biphenyls (7-10) through ring cleavage and consequent cyclization reaction. Bis-amide (11), and Schiff-base (12) were synthesized from oxindoles respectively. The investigation of cytotoxicity of the synthesized compounds was carried out against brine shrimp lethality bioassay. In our present investigation some of the synthesized compounds such as halogen substituted acetylisatins (4-6) and the ring extended diazo-biphenyls, (7-10) have been shown to exhibit strong cytotoxic effect. Key words: diazo-biphenyls; oxindoles; cytotoxicity; brine shrimp DOI: 10.3329/dujps.v9i1.7423Dhaka Univ. J. Pharm. Sci. 9(1): 1-6, 2010 (June)

Cytotoxicity Study of Some Ketone Schiff Bases and Heterocyclic Compounds

1970 ◽  
Vol 4 (1) ◽  
Author(s):  
Mohammad Al-Amin ◽  
Md Rabiul Islam
Keyword(s):  
Schiff Base ◽  
Cytotoxic Activity ◽  
Key Words ◽  
Schiff Bases ◽  
Full Text ◽  
Brine Shrimp ◽  
Heterocyclic Compounds ◽  
Brine Shrimp Lethality

The cytotoxicity of 11 synthesized ketone Schiff bases and 16 heterocyclic compounds was evaluated by the brine shrimp lethality bioassay. It was observed that all the synthesized compounds showed significant cytotoxic activity with LC50 of 1.05-2.45 µg/ml against brine shrimp nauplii. Key words: Schiff base, Heterocyclic compounds, Cytotoxicity, Brine shrimp. Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

scholarly journals Cytotoxic Activity of Two Limonoids Isolated From Swietenia mahagoni by Using Brine Shrimp Lethality Bioassay

1970 ◽  
pp. 1-6
Author(s):  
AKM Shahidur Rahman ◽  
AK Azad Chowdhury ◽  
Hossain Reza ◽  
Mohammad Shawkat Ali ◽  
Manzurur Rahman Shah Choudhury ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Key Words ◽  
Column Chromatography ◽  
Brine Shrimp ◽  
Meoh Extract ◽  
Brine Shrimp Lethality ◽  
Swietenia Mahagoni

Solvent partitioning followed by column chromatography of the MeOH extract of the seeds of Swietenia mahagoni afforded two limonoids, swietenolide (compound 1) and 2-hydroxy-3-O-tigloylswietenolide (compound 2), later one is new compound. The compounds were identified by spectroscopic means. The cytotoxic activity of these compounds was assessed by using the conventional brine shrimp lethality bioassay. While both compounds were found to have moderate cytotoxic activity, compound 2 displayed overall more potent activity than compound 1. Key words: Cytotoxic; Limonoid; Swietenia mahagoni; Brine shrimp lethality bioassay DOI: 10.3329/bjpp.v24i1.5729Bangladesh J Physiol Pharmacol 2008; 24(1&2) : 1-6

scholarly journals In Vitro Anticancer MCF-7, Anti-Inflammatory, and Brine Shrimp Lethality Assay (BSLA) and GC-MS Analysis of Whole Plant Butanol Fraction of Rheum ribes (WBFRR)

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Jahangir Khan Achakzai ◽  
Muhammad Anwar Panezai ◽  
Muhammad Ayub Kakar ◽  
Abdul Manan Kakar ◽  
Shahabuddin Kakar ◽  
...  
Keyword(s):  
Cell Line ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Aqueous Fraction ◽  
Brine Shrimp Lethality ◽  
Butanol Fraction ◽  
Whole Plant ◽  
Brine Shrimp Lethality Assay ◽  
Anti Inflammatory ◽  
Mcf 7

In this study, GC-MS analysis has shown that whole plant butanol fraction of rheum ribes (WBFRR) comprises of 21 compounds which exhibited anticancer (MCF-7) activity having IC50 value of 36.01± 0.26. MTT assay (MCF-7), Oxidative Burst assay using chemiluminescence technique, and B-Hatching techniques were the methods used for anticancer MCF-7, anti-inflammatory, and Brine Shrimp Lethality Assay (BSLA). GC-MS was used for structural elucidation. Whole plant methanol extract of rheum ribes (WMERR), whole plant n-hexane fraction of rheum ribes (WHFRR), and whole plant aqueous fraction of rheum ribes (WAFRR) were inactive against anticancer (MCF-7) cell line. Whole plant methanol extract of rheum ribes (WMERR), whole plant aqueous fraction of rheum ribes (WAFRR) and whole plant butanol fraction of rheum ribes (WBFRR) showed anti-inflammatory activity on ROS having IC50 value of 23.2±1.9, 24.2±2.7 and 12.0±0.6. Whole plant butanol fraction of rheum ribes (WBFRR) showed Brine Shrimp Lethality with LD50 693.302 while whole plant methanol extract of rheum ribes (WMERR) and whole plant aqueous fraction of rheum ribes (WAFRR) showed high lethality at highest concentration. This study revealed that whole plant butanol fraction of rheum ribes (WBFRR) exhibited significant anticancer (MCF-7) activity. In the near future, the constituent of whole plant butanol fraction of rheum ribes (WBFRR) can be the alternative drug against MCF-7 cell line with least toxicity and side effects.

scholarly journals Uji Aktivitas Sitotoksik dan Analisis Fitokimia Ekstrak Etanol Daun Sampare (Glochidion sp.)

2018 ◽  
Vol 9 (2) ◽  
pp. 49-54
Author(s):  
Ganis Oktalia ◽  
Linus Y. Chrystomo ◽  
Aditya K. Karim
Keyword(s):  
Herbal Medicine ◽  
Key Words ◽  
Brine Shrimp ◽  
Chemical Substance ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
In Vivo Test ◽  
Phytochemical Compounds

Sampare (Glochidion sp., Family: Phyllantaceae) is a plant which grows dominant in Biak, Papua. Based on the local wisdom of the community, by hereditary, it can be used as an herbal medicine to treat the malaria disease. This study aims to determine the toxicity of LC50 and to know the phytochemical compounds of extracted leaf of Glochidion sp using ethanol. The Brine Shrimp Lethality Test (BSLT) and phytochemical screening using reagent chemical substance methods were used in this research. The results of this study can be concluded that the toxicity of extracted leaf of Glochidion sp. using ethanol was low, with LC50 = 758.58 ppm and the results of phytochemical analysis showed the existence of the content of alkaloids, flavonoids, saponins, tannins and quinones. But, the steroids and triterpenoids could not be found in the extracted leaf of Glochidion sp using ethanol. Therefore, for further research is needed to isolate the bioactive compounds and in vivo test on the plasmodium, which cause the malaria disease. Key words: Glochidion sp., malaria desease, Brine Shrimp Lethality Test (BSLT), LC50, phytochemical analysis

Antitumor and cytotoxic effect of different partitionates of methanol extract of Trema orientalis : A preliminary in-vitro study

2018 ◽  
Vol 3 (4) ◽  
Author(s):  
Riadh Hasan Rana ◽  
Md. Shah Amran ◽  
Abu Asad Chowdhury
Keyword(s):  
Antitumor Activity ◽  
Ethyl Acetate ◽  
Plant Extract ◽  
Brine Shrimp ◽  
Cytotoxic Effect ◽  
Methanol Extract ◽  
Tumor Formation ◽  
Brine Shrimp Lethality ◽  
Bioassay Method ◽  
Potato Disc

Herbal drugs are widely used in the primary health care system now-a-days and the use is increasing day by day. Thus extensive research to confirm the bioactivity of plant extract is very essential. The aim of this research was to determine the antitumor and cytotoxic actions of solvent-solvent partitioned fraction (n-hexane, ethyl acetate and hydro-methanol) of the methanol extract of root of Tremaorientalis. The antitumor activity of different fractions of plant extract was evaluated by potatodisc bioassay method where Agrobacterium tumefaciens was used to induce tumor on potato disc. On the other hand, the cytotoxic effect was determined by brine shrimp lethality bioassay technique. The ethyl acetate fraction of the plant extract showed 35.51% and 62.89% inhibition of tumor formation in potato disc bioassay method at 50 µg/disc and 100 µg/disc respectively. The hydro-methanol extract showed 37.51% inhibition of tumor formation at 100 µg/disc while at 50 µg/disc no significant inhibition was observed. In contrast, n-hexane fraction did not show any antitumor activity. In cytotoxic study by brine shrimp lethality bioassay, the lethal concentration-50 (LC50) value of the n-hexane, ethyl acetate and hydro-methanol fraction was 1377.03 µg/ml, 11.67 µg/ml and 48.62 µg/ml respectively. Ethyl acetate and hydro-methanol fractions of the solvent-solvent partitioned methanol extract of T. orientalis showed high antitumor and cytotoxic effect. These fractions are highly promising for further exploration to identify the bioactive compounds.

scholarly journals In Vitro Anti-Inflammatory, Anticancer (MCF-7, 3T3, and HeLa Cell Lines), and Brine Shrimp Lethality Assay and FTIR Analysis of the Extract and Fractions of the Whole Plant of Heliotropium europaeum

2020 ◽  
Vol 2020 ◽  
pp. 1-14
Author(s):  
Jahangir Khan Achakzai ◽  
Muhammad Anwar Panezai ◽  
Basira Akhtar ◽  
Shahabuddin Kakar ◽  
Ali Akbar ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Ftir Analysis ◽  
Brine Shrimp Lethality ◽  
Ftir Studies ◽  
Whole Plant ◽  
Brine Shrimp Lethality Assay ◽  
Hela Cell Lines ◽  
Heliotropium Europaeum ◽  
Anti Inflammatory ◽  
Mcf 7

In this study, anti-inflammatory, anticancer, brine shrimp lethality, and FTIR studies were evaluated. The oxidative burst assay using the chemiluminescence technique, MTT assay, brine shrimp lethality assay, and FTIR analysis were the methods used for the evaluation of anti-inflammatory, anticancer, brine shrimp lethality, and FTIR studies, respectively. The whole-plant butanol fraction of Heliotropium europaeum (WBFHE) showed anti-inflammatory activity on ROS having IC5014.7±2.5 while the extract and other fractions of the whole plant of Heliotropium europaeum exhibited no anti-inflammatory activity. None of the extract and fractions of the whole plant of Heliotropium europaeum exhibited anticancer (MCF-7, 3T3, and HeLa cell lines) activities. The whole-plant aqueous fraction of Heliotropium europaeum (WAFHE) and whole-plant butanol fraction of Heliotropium europaeum (WBFHE) showed lethality at high concentration while at low concentration, no toxicity was shown. The whole-plant methanolic extract of Heliotropium europaeum (WMEHE) and whole-plant n-hexane fraction of Heliotropium europaeum (WHFHE) exhibited no toxicity. FTIR interpretation showed the functional groups for the aromatic compounds, phenols, carboxylic acids, esters, alkanes, alkenes, alcohols, alkyl halides, sulfate esters, phosphines, silanes, nitriles, thiols, amines, phosphoric acids, and nitro compounds.

A Benzofuranone from the Flowers of Nyctanthes arbortristis and its Antibacterial and Cytotoxic Activities

1970 ◽  
Vol 4 (1) ◽  
Author(s):  
Naznin Ara Khatune ◽  
M Asik Mossadik ◽  
M Mukhlesur Rahman ◽  
Proma Khondkar ◽  
M Ekramul Haque ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Key Words ◽  
Full Text ◽  
Brine Shrimp ◽  
2D Nmr ◽  
Cytotoxic Activities ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Brine Shrimp Lethality

A benzofuranone, 3,3a,7,7a-tetrahydro-3a-hydroxy-6(2H)-benzofuranone (1), was isolated from the flower of Nyctanthes arbortristis Linn. The compound was identified by 1D and 2D NMR and MS analyses. It showed significant antibacterial activity against both Gram positive and Gram negative bacteria. The LC50 of the compound was found to be 8.73 mg/ml in brine shrimp lethality bioassay. Key words: Nyctanthes arbortristis, Nyctantheceae; 3,3a,7,7a-tetrahydro-3a-hydroxy-6(2H)-benzofuranone, Antibacterial, Cytotoxicity Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

scholarly journals Cytotoxicity and Brine Shrimp Lethality of Rotenoids and Extracts from Sarcolobus globosus

2007 ◽  
Vol 2 (8) ◽  
pp. 1934578X0700200 ◽  
Author(s):  
Helle Wangensteen ◽  
Mahiuddin Alamgir ◽  
Sultana Rajia ◽  
Trine J. Meza ◽  
Anne Berit Samuelsen ◽  
...  
Keyword(s):  
Brine Shrimp ◽  
Cytotoxic Effect ◽  
High Toxicity ◽  
Mangrove Plant ◽  
Brine Shrimp Lethality ◽  
Brine Shrimp Toxicity

The present study was performed to examine the brine shrimp toxicity and cytotoxic effect of the mangrove plant Sarcolobus globosus. The Et2O and EtOAc extracts were toxic to brine shrimp larvae (LC50 = 1.6 and 4.0 μg/mL) and Caco-2 cells (IC50 = 6.7 and 21.2 μg/mL). Three rotenoids isolated from S. globosus, tephrosin, sarcolobin and 12a-hydroxyrotenone, showed high toxicity in the brine shrimp assay with LC50 values of 2.2, 2.8 and 1.9 μM, respectively.

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