Cytotoxicity Study of Some Ketone Schiff Bases and Heterocyclic Compounds

1970 ◽  
Vol 4 (1) ◽  
Author(s):  
Mohammad Al-Amin ◽  
Md Rabiul Islam
Keyword(s):  
Schiff Base ◽  
Cytotoxic Activity ◽  
Key Words ◽  
Schiff Bases ◽  
Full Text ◽  
Brine Shrimp ◽  
Heterocyclic Compounds ◽  
Brine Shrimp Lethality

The cytotoxicity of 11 synthesized ketone Schiff bases and 16 heterocyclic compounds was evaluated by the brine shrimp lethality bioassay. It was observed that all the synthesized compounds showed significant cytotoxic activity with LC50 of 1.05-2.45 µg/ml against brine shrimp nauplii. Key words: Schiff base, Heterocyclic compounds, Cytotoxicity, Brine shrimp. Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

scholarly journals Synthesis and Cytotoxicity Study of Some Acetylisatins and Diazobiphenyls

1970 ◽  
Vol 9 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Mohammad Mamun Hossain ◽  
Sakanta Kumar Shaha ◽  
Foysal Aziz ◽  
Rahat Khan ◽  
Md Mahabub Hossain
Keyword(s):  
Schiff Base ◽  
Key Words ◽  
Brine Shrimp ◽  
Heterocyclic Compounds ◽  
Cytotoxic Effect ◽  
Cyclization Reaction ◽  
Ring Cleavage ◽  
Anticancer Activities ◽  
Brine Shrimp Lethality ◽  
Anti Inflammatory

Nitrogenous heterocyclic compounds, oxindoles especially isatins and related heterocyles have excellent antifungal, anti-inflammatory, antimicrobial and anticancer activities. Oxindoles keto lactams (1-3) were synthesized according to modified Sandmayer method and their N-acetylated compounds (4-6) gave diazo-biphenyls (7-10) through ring cleavage and consequent cyclization reaction. Bis-amide (11), and Schiff-base (12) were synthesized from oxindoles respectively. The investigation of cytotoxicity of the synthesized compounds was carried out against brine shrimp lethality bioassay. In our present investigation some of the synthesized compounds such as halogen substituted acetylisatins (4-6) and the ring extended diazo-biphenyls, (7-10) have been shown to exhibit strong cytotoxic effect. Key words: diazo-biphenyls; oxindoles; cytotoxicity; brine shrimp DOI: 10.3329/dujps.v9i1.7423Dhaka Univ. J. Pharm. Sci. 9(1): 1-6, 2010 (June)

scholarly journals Cytotoxic Activity of Two Limonoids Isolated From Swietenia mahagoni by Using Brine Shrimp Lethality Bioassay

1970 ◽  
pp. 1-6
Author(s):  
AKM Shahidur Rahman ◽  
AK Azad Chowdhury ◽  
Hossain Reza ◽  
Mohammad Shawkat Ali ◽  
Manzurur Rahman Shah Choudhury ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Key Words ◽  
Column Chromatography ◽  
Brine Shrimp ◽  
Meoh Extract ◽  
Brine Shrimp Lethality ◽  
Swietenia Mahagoni

Solvent partitioning followed by column chromatography of the MeOH extract of the seeds of Swietenia mahagoni afforded two limonoids, swietenolide (compound 1) and 2-hydroxy-3-O-tigloylswietenolide (compound 2), later one is new compound. The compounds were identified by spectroscopic means. The cytotoxic activity of these compounds was assessed by using the conventional brine shrimp lethality bioassay. While both compounds were found to have moderate cytotoxic activity, compound 2 displayed overall more potent activity than compound 1. Key words: Cytotoxic; Limonoid; Swietenia mahagoni; Brine shrimp lethality bioassay DOI: 10.3329/bjpp.v24i1.5729Bangladesh J Physiol Pharmacol 2008; 24(1&2) : 1-6

A Benzofuranone from the Flowers of Nyctanthes arbortristis and its Antibacterial and Cytotoxic Activities

1970 ◽  
Vol 4 (1) ◽  
Author(s):  
Naznin Ara Khatune ◽  
M Asik Mossadik ◽  
M Mukhlesur Rahman ◽  
Proma Khondkar ◽  
M Ekramul Haque ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Key Words ◽  
Full Text ◽  
Brine Shrimp ◽  
2D Nmr ◽  
Cytotoxic Activities ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Brine Shrimp Lethality

A benzofuranone, 3,3a,7,7a-tetrahydro-3a-hydroxy-6(2H)-benzofuranone (1), was isolated from the flower of Nyctanthes arbortristis Linn. The compound was identified by 1D and 2D NMR and MS analyses. It showed significant antibacterial activity against both Gram positive and Gram negative bacteria. The LC50 of the compound was found to be 8.73 mg/ml in brine shrimp lethality bioassay. Key words: Nyctanthes arbortristis, Nyctantheceae; 3,3a,7,7a-tetrahydro-3a-hydroxy-6(2H)-benzofuranone, Antibacterial, Cytotoxicity Dhaka Univ. J. Pharm. Sci. Vol.4(1) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

scholarly journals UJI TOKSISITAS DARI EKSTRAK LAMUN JENIS Thalassia hemprichii DARI PERAIRAN KALASEY DENGAN MENGGUNAKAN METODE BRINE SHRIMP LETHALITY TEST

2019 ◽  
Vol 7 (3) ◽  
pp. 265
Author(s):  
Faradila Y. Karim ◽  
Nickson J Kawung ◽  
Billy Th. Wagey
Keyword(s):  
Cytotoxic Activity ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Bioactive Compound ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Thalassia Hemprichii ◽  
Brine Shrimp Lethality ◽  
A Value ◽  
Small Doses

ABSTRACTBioactive compounds that are suspected of having anticancer activity are first tested for activity by means of a toxicity test. The purpose of this test is to obtain data on the ability of the activity of a bioactive compound to kill cells at small doses so as to obtain a lethal concentration or lethal data. These two measurements are often called LC50 or LD50, concentrations that can kill 50% of test animals. This study aims to test the cytotoxic activity of Thalassia hemprichii seagrass extract using the Brine Shrimp Lethality Test (BSLT) method.The results of the study showed that the mortality of Artemia salina larvae was influenced by the concentration of the test, meaning that the higher the concentration the more test animals died. Furthermore, the results of the probit analysis contained the toxicity activity of bio active compounds from seagrasses where the LC50 was 3.95 mg / l. A substance is declared to have the potential for cytotoxic activity if it has a value of LC50 at concentrations <1000 ppm for extracts and at concentrations <30 ppm for a compound. Keywords: Cytotoxic Activity, Thalassia hemprichii, Brine shrimp Lethality Test (BSLT)  

scholarly journals Steroids from the Leave of Persicaria perfoliata (L.) H. Gross

2019 ◽  
Vol 22 (2) ◽  
pp. 224-227
Author(s):  
Md Sagar Hossain ◽  
NM Mofiz Uddin Khan ◽  
Md Saiful Quddus ◽  
AM Sarwaruddin Chowdhury
Keyword(s):  
Antimicrobial Activity ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Pharmaceutical Journal ◽  
Antimicrobial Screening ◽  
Brine Shrimp Lethality ◽  
Persicaria Perfoliata ◽  
Test Organisms

β-sitosterol (1) and β-sitosterol-D-glucoside (2) were isolated from the dichloromethane soluble fraction of a methanol extract of the leave of Persicaria perfoliata. The crude fraction was subjected to antimicrobial screening and brine shrimp lethality bioassay, where moderate antimicrobial activity was observed against most of the test organisms was seen. The hexane soluble fraction also displayed significant cytotoxic activity with LC50 0.64 μg/ml in brine shrimp lethality bioassay. This is the first report of occurrence of these compounds from this plant. Bangladesh Pharmaceutical Journal 22(2): 224-227, 2019

scholarly journals Antioxidant, Antibacterial and Cytotoxic Activity of the Methanol Extract of Urtica Crenulata

2009 ◽  
Vol 2 (1) ◽  
pp. 169-177
Author(s):  
M. A. Rahman ◽  
M. S. Rana ◽  
M. M. Zaman ◽  
S. A. Uddin ◽  
R. Akter
Keyword(s):  
Ascorbic Acid ◽  
Free Radical ◽  
Cytotoxic Activity ◽  
Brine Shrimp ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Brine Shrimp Lethality ◽  
Brine Shrimp Lethality Assay ◽  
Stem Extract

The antioxidant, antibacterial and cytotoxic properties of methanol extract of Urtica crenulata (syn: Laportea crenulata Gaud) stem has been investigated in the present study. The antioxidant and cytotoxic activity of the Urtica crenulata methanol extract were assessed by DPPH free radical scavenging method and brine shrimp lethality bioassay method, respectively. The antibacterial activity of the plant extract (500 μg/disc) was also carried out by disc diffusion technique. Stem extract showed DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging effect compared with ascorbic acid. IC50 value of ascorbic acid and stem extract was found 14.72 μg/ml and 1468.9 μg/ml, respectively. In antibacterial experiment, Urtica crenulata stem extract showed 8, 14 and 10 mm of diameter of zone inhibition against Salmonella typhi, Shigella flexneri and Shigella sonnei, respectively and 9 and 8 mm of diameter of zone inhibition against Bacillus subtilis and Bacillus cereus but no activity was observed against Staphylococcus aureus. In brine shrimp lethality assay, the LC50 value of the extract was found 104.0 μg/ml, which indicates that the extract has high cytoxic effect. The present study demonstrates that methanol extract of Urtica crenulata stem has significant cytotoxic effect. The extract also showed some moderate antibacterial and minimum significant antioxidant effects.  Keywords: Urtica crenulata;  Antioxidant; Antibacterial; Cytotoxic; BHT. © 2010 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v2i1.2872             J. Sci. Res. 2 (1), 169-177 (2010) 

scholarly journals SYNTHESIS, CHARACTERIZATION, QUANTITATIVE STRUCTURE-ACTIVITY RELATIONSHIP, DOCKING, ANTIBACTERIAL ACTIVITY, AND BRINE SHRIMP LETHAL STUDIES ON L-PHENYLALANINE SCHIFF BASES

2016 ◽  
Vol 9 (9) ◽  
pp. 203
Author(s):  
Easwaramoorthi Deivanayagam ◽  
Jayaprakash R ◽  
Aroj Kumar Sha ◽  
Hemalatha S
Keyword(s):  
Antibacterial Activity ◽  
Schiff Base ◽  
Schiff Bases ◽  
Brine Shrimp ◽  
Quantitative Structure Activity Relationship ◽  
Structure Activity Relationship ◽  
Activity Relationship ◽  
Hydroxyl Group ◽  
Quantitative Structure ◽  
Structure Activity

ABSTRACTObjective: Aim of this work is to synthesize and characterization of the hydroxyl group the hydroxyl group substituted L-phenylalanine Schiff basesto compare their predicted quantitative structure-activity relationship (QSAR) and molecular docking against Escherichia coli protein ZipA (1s1j)outcomes with the antibacterial activity and brine shrimp lethal assay (BSLA) results.Methods: The Schiff bases of L-Phenylalanine were synthesized by the simple condensation reaction using methanol, water in 2:1 ratio at reflux andwere characterized by spectral techniques. QSAR parameters of the Schiff bases were predicted using java-based online and offline tools. Moleculardocking carried through online mcule server and CLC Drug Discovery Workbench 3. Antibacterial activity and toxicity studies were conducted usingbroth dilution and brine shrimp lethal assay methods, respectively.Results: The Schiff bases fulfilled the QSAR drug-likeness parameters and showed the docking score between −6.8 and −6.0 Kcal/mol which arehigher than amoxilicillin and gentamicin like standard drugs. They also possess good inhibition for urinary tract infection causing E. coli bacteria,and minimum inhibitory concentrations (MIC) exists between 3.25 and 5.25 μg/ml. The brine shrimp lethal concentration for 50% mortality [LC50])between 58.73 and 135.6 μg/ml.Conclusion: Para, meta and 2,4 hydroxyl substituted Schiff bases exhibited good inhibition against Gram-negative E. coli bacteria at low concentrationand the MIC exists below the LC50 value. The Schiff base showed high drug score, high docking score, and low toxicity than other Schiff base. Dockingscore, high inhibition, low clogP, low MICKeywords: L-phenylalanine, Schiff base, Quantitative structure-activity relationship, Docking, Antibacterial, Lethal concentration for 50% mortality.

scholarly journals Phytochemical and Biological Investigations of Phyllanthus reticulatus

1970 ◽  
Vol 5 (1) ◽  
pp. 21-23 ◽  
Author(s):  
Taslima Begum ◽  
Mohammad S Rahman ◽  
Mohammad A Rashid
Keyword(s):  
Carbon Tetrachloride ◽  
Inhibitory Activity ◽  
Full Text ◽  
Brine Shrimp ◽  
Microbial Growth ◽  
Methanol Extract ◽  
Soluble Fraction ◽  
Stem Bark ◽  
Brine Shrimp Lethality ◽  
Soluble Fractions

Scopoletin (1) was isolated from the chloroform soluble fraction of a methanol extract of the stem bark of Phyllanthus reticulates (Family: Euphorbiaceae). The petroleum ether, carbon tetrachloride and choloroform soluble fractions of this methanol extract were subjected to antimicrobial screening and brine shrimp lethality bioassay. All of the partitionates showed moderate to strong inhibitory activity to microbial growth while the chloroform soluble fraction showed strongest cytotoxicity having LC50 1.99 ?g/ml. Key words: Phyllanthus reticulates, Euphorbiaceae, Scopoletin, Brine shrimp lethality bioassay, Antimicrobial Dhaka Univ. J. Pharm. Sci. Vol.5(1-2) 2006 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

scholarly journals Uji Aktivitas Sitotoksik dan Analisis Fitokimia Ekstrak Etanol Daun Sampare (Glochidion sp.)

2018 ◽  
Vol 9 (2) ◽  
pp. 49-54
Author(s):  
Ganis Oktalia ◽  
Linus Y. Chrystomo ◽  
Aditya K. Karim
Keyword(s):  
Herbal Medicine ◽  
Key Words ◽  
Brine Shrimp ◽  
Chemical Substance ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Brine Shrimp Lethality ◽  
In Vivo Test ◽  
Phytochemical Compounds

Sampare (Glochidion sp., Family: Phyllantaceae) is a plant which grows dominant in Biak, Papua. Based on the local wisdom of the community, by hereditary, it can be used as an herbal medicine to treat the malaria disease. This study aims to determine the toxicity of LC50 and to know the phytochemical compounds of extracted leaf of Glochidion sp using ethanol. The Brine Shrimp Lethality Test (BSLT) and phytochemical screening using reagent chemical substance methods were used in this research. The results of this study can be concluded that the toxicity of extracted leaf of Glochidion sp. using ethanol was low, with LC50 = 758.58 ppm and the results of phytochemical analysis showed the existence of the content of alkaloids, flavonoids, saponins, tannins and quinones. But, the steroids and triterpenoids could not be found in the extracted leaf of Glochidion sp using ethanol. Therefore, for further research is needed to isolate the bioactive compounds and in vivo test on the plasmodium, which cause the malaria disease. Key words: Glochidion sp., malaria desease, Brine Shrimp Lethality Test (BSLT), LC50, phytochemical analysis

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