scholarly journals In Vitro Evaluation of Selected Plant extracts and Chemicals against Pathogenic Fungi isolated from Momordica Charantia L.

2017 ◽  
Vol 41 (1) ◽  
pp. 11-16
Author(s):  
Md Dulal Miya ◽  
Shamim Shamsi
Keyword(s):  
Sodium Chloride ◽  
Sodium Bicarbonate ◽  
Growth Inhibition ◽  
Plant Extracts ◽  
Radial Growth ◽  
Pathogenic Fungi ◽  
Momordica Charantia ◽  
Rhizopus Stolonifer ◽  
Fresh Vegetables ◽  
Fusarium Sp

Five plant extracts and two chemicals were evaluated against radial growth of five pathogenic fungi isolated from fresh vegetables of two varieties of Momordica charantia L. The isolated fungi were Aspergillus niger Van Tiegh, Curvularia brachyspora Boedijn, Fusarium Link, Rhizopus stolonifer (Ehrenb.:Fr.) Vuill and Trichoderma viride Pers.. Five plant extracts namely Allium sativum L., Azadirachta indicia A. Juss., Citrus limon (L.) Burm. f, Mangifera indica L. and Psidium guajava L. were evaluated for inhibiting growth of above mentioned fungi associated with the vegetable. Out of the five plant extracts, A. sativum showed complete radial growth inhibition of A. niger, C. brachyspora, Fusarium sp. and R. stolonifer at 15 and 20% concentrations. Azadirachta indica showed complete radial growth inhibition of C. brachyspora and Fusarium sp. Rhizopus stolonifer was completely inhibited by 15 and 20% concentration owing to leaf extract of A. indica and P. guajava. The same fungus was completely inhibited by M. indica at 20% concentration. Fusarium sp. was completely inhibited by C. limon at 20% concentration. Two chemicals, sodium bicarbonate and sodium chloride were used against the test pathogens and sodium bicarbonate showed complete inhibition of radial growth of A. niger, C. brachyspora, Fusarium sp. and R. stolonifer at 20% concentration. The last one also inhibited by the chemical at 15% concentration. Sodium chloride showed highest 95% inhibition of radial growth of T. viride at 20% concentration.Journal of Bangladesh Academy of Sciences, Vol. 41, No. 1, 11-16, 2017

scholarly journals In Vitro Screening of Fungicides and Plant Extracts Against Pathogenic Fungi Associated With Infected Fruits of Carica Papaya L

2018 ◽  
Vol 42 (2) ◽  
pp. 121-128
Author(s):  
M Rezuana Binte Helal ◽  
Shamim Shamsi
Keyword(s):  
Growth Inhibition ◽  
Plant Extracts ◽  
Radial Growth ◽  
Carica Papaya ◽  
Curcuma Longa ◽  
Pathogenic Fungi ◽  
Ocimum Sanctum ◽  
Fusarium Sp ◽  
Thuja Orientalis

Five fungicides viz., Autostin 50 WDG, Dithane M 45, Greengel 72 WP, Ridomil Gold MZ 68 WG   and Tilt 250 EC exclusively were tested against three pathogenic fungi viz., Colletotrichum gloeosporioides (Penz.) Sacc., Fusarium nivale (Fr.) Ces. and Fusarium sp. associated with Carica papaya L. following poisoned food technique. At 100 ppm  Autostin showed complete growth inhibition of C. gloeosporioides, F. nivale and Fusarium sp., while  Tilt 250 EC showed complete growth inhibition of C. gloeosporioides at the same concentration. These fungicides at 400 and 500 ppm showed complete growth inhibition of F. nivale. Tilt 250 EC showed complete growth inhibition of Fusarium sp. at 300, 400 and 500 ppm. Eight plants extract, viz., Adhatoda vasica, Azadirachta indica, Curcuma longa, Ocimum sanctum, Lantana camara, Tagetes erecta, Thuja orientalis and Vitex negundo were also tested for their  efficacy at 5, 10, 15 and 20% concentrations against these pathogenic fungi. Out of the eight plant extracts, Ocimum sanctum showed highest (88.57%) radial growth inhibition of C. gloeosporioides at 20% concentration. Curcuma longa showed highest (88.55%) radial growth inhibition of F. nivale and Adhatoda vesica showed highest (80.02%) radial growth inhibition of Fusarium sp. at the same concentrations. The present investigation suggests Autostin 50 WDG and Tilt 250 EC as best inhibiting chemical fungicides for C. gloeosporioides, F. nivale and Fusarium sp. Extracts of O. sanctum, C. longa and Adhatoda vesica were found to be superior to other plant extracts tested. Journal of Bangladesh Academy of Sciences, Vol. 42, No. 2, 121-128, 2018

scholarly journals Antagonistic potentials of some soil fungi against three post‐harvest pathogenic fungi of Carica papaya L.

2019 ◽  
Vol 28 (1) ◽  
pp. 1-7
Author(s):  
Rezuana Binte Helal ◽  
Shamim Shamsi
Keyword(s):  
Growth Inhibition ◽  
Radial Growth ◽  
Soil Fungi ◽  
Carica Papaya ◽  
Trichoderma Viride ◽  
Pathogenic Fungi ◽  
Dual Culture ◽  
Volatile Metabolites ◽  
Maximum Inhibition ◽  
Fusarium Sp

Four antagonistic fungi were isolated from the papaya field soil by serial dilution and were identified as Aspergillus flavus Link., A. fumigatus Fresenius, A. niger van Tiegh. and Trichoderma viride Pers. The soil fungi were selected to evaluate their antagonistic potentiality against the three postharvest pathogenic fungi of Carica papaya L. following "dual culture colony interaction” and volatile and non-volatile metabolites. In dual culture colony interaction, out of four soil fungi, T. viride showed the highest growth inhibition on C. gloeosporioides (84.28%), F. nivale (95.23%) and Fusarium sp. (87.15%). On the other hand A. niger showed the highest growth inhibition on C. gloeosporioides (77.39%), F. nivale (98.63%) and Fusarium sp. (35.05%). The maximum inhibition of radial growth of C. gloeosporioides (77.64%), F. nivale (58.76%) and Fusarium sp. (79.37%). were observed in case of T. viride owing to the volatile metabolites. Whereas the maximum inhibition of radial growth of C. gloeosporioides (58.23%), F. nivale (37.43%) and Fusarium sp. (82.31%) were observed in case of A. niger owing to the volatile metabolites. The maximum inhibition of radial growth of C. gloeosporioides (90.90%), F. nivale (89.13%) and Fusarium sp. (76.84%) were observed in case of T. viride owing to the effect of non-volatile metabolites. The maximum inhibition of radial growth of C. gloeosporioides (92.42%), F. nivale (73.01%) and Fusarium sp. (68.67%) were observed in case of A. niger owing to the effect of non-volatile metabolites. Trichoderma viride and A. niger may be exploited commercially as a biocontrol agent against anthracnose and fruit rot pathogens of papaya. Dhaka Univ. J. Biol. Sci. 28(1): 1-7, 2019 (January)

In-vitro Studies of Plant Extracts against Alternaria brassicae (berk.) Sacc. of Indian Mustard (Brassica juncea (L.) Czern. and coss)

2016 ◽  
Vol 3 (1) ◽  
Author(s):  
MAHESH SINGH ◽  
H. K. SINGH
Keyword(s):  
Growth Inhibition ◽  
Plant Extract ◽  
Plant Extracts ◽  
Indian Mustard ◽  
Pathogenic Fungi ◽  
Alternaria Brassicae ◽  
Test Fungus ◽  
Poison Food Technique ◽  
Percent Growth Inhibition

Six plant extract viz., Datura (Daturastramonium), Eucalyptus (Eucalyptus globulous), Karang (Pongamiaglabra),Neem (Azadirachtaindica), Madar (Calotropis gigantean) and Wild sage (Lantana camara) were evaluated in-vitro by poison food technique @ 3, 6, 9 and 12% concentrations against Alternariabrassicaecausing blight of mustard. The results revealed that all the plant extracts inhibited the percent growth inhibition against test fungus as compared to control. However A.indica was found most efficacious with growth inhibition of (38.7%) followed by E. globulus (36.7%). Some extract such as P. glabra and D. stramonium showed moderate inhibition over control (30.9 and 21.6 respectively). Among all plant extract, C. gigantean showed least (15.3%) inhibition over control against A. brassicae. These plant extracts can possibly be used as management of seedborne pathogenic fungi in an eco-friendly way.

scholarly journals In vitro evaluation of fungicides and plant extracts against pathogenic fungi of two rice varieties

2018 ◽  
Vol 44 (2) ◽  
pp. 251-259
Author(s):  
Pranami Chowdhury ◽  
MA Bashar ◽  
Shamim Shamsi
Keyword(s):  
Plant Extracts ◽  
Radial Growth ◽  
Fusarium Moniliforme ◽  
Mangifera Indica ◽  
Pathogenic Fungi ◽  
Curvularia Lunata ◽  
Asparagus Racemosus ◽  
Rice Varieties ◽  
Datura Metel

Five pathogenic fungi viz., Alternaria alternata (Fr.) Keissler, Curvularia lunata (Wakker) Boedijn, Drechslera oryzae Breda de Haan (Subramanian and Jain), Fusarium moniliforme Sheldon and Pestalotiopsis guepinii (Desm.) Stay. were isolated from two rice varieties viz., BRRI 29 (Boro) and Pajam (Aman) by Blotter and Tissue planting methods. Ten fungicides i.e. Bavistin 50 WP, Salcox 50 WP, Dithane M-45, Indofil M-45, Tall 25 EC, Ridomil MZ Gold, MC Sulphur 80, Greengel, Hayvit 80 WP and Capvit 50 WP at 100, 200, 300, 400 and 500 ppm were evaluated against the above mentioned five pathogenic fungi. Tall 25 EC completely inhibited the radial growth of the test fungi at all the concentrations except Fusarium moniliforme. Antifungal properties of ethanol extract of Artocarpus heterophyllus Lamk., Tagetes erecta L., Datura metel L., Senna alata (L.) Roxb., Azadirachta indica A. Juss., Citrus medica L., Mangifera indica L., Asparagus racemosus Willd., Nerium indicum Mill. and Allium sativum L. at 5, 10 and 20% concentrations were evaluated against the five test pathogens. All the plant extracts completely inhibited the radial growth of the test fungi at 20% concentration except Asparagus racemosus.

scholarly journals Efficacy of some plant extracts on the mycelial growth of Colletotrichum gloeosporioides

1970 ◽  
Vol 9 (1) ◽  
pp. 43-48 ◽  
Author(s):  
A Mukherjee ◽  
S Khandker ◽  
MR Islam ◽  
Sonia B Shahid
Keyword(s):  
Growth Inhibition ◽  
Plant Extracts ◽  
Growth And Development ◽  
Radial Growth ◽  
Mycelial Growth ◽  
Colletotrichum Gloeosporioides ◽  
Causal Agent ◽  
Garlic Extract ◽  
Tobacco Leaf ◽  
Anti Fungal

The anti fungal effects of some plants extracts namely tobacco leaf, keora seed, keora, mahogoni, gaint indian milky weed, garlic and ginger at different concentrations (30%, 40%, 50%, 60% and 70%) on the growth and development of C. gloeosporioides, causal agent of anthracnose of mango were evaluated. Radial growth of C. gloeosporioides was recorded. The growth inhibition increase with the increase of concentration of all the plant extracts. Highest mycelial growth inhibition (74.35%) was observed in case of garlic extracts at 70% concentration. Garlic extract at 50% and 60% concentration were also effective than other treatments.Keywords: C. Gloeosporiodes; Plant extracts; Inhibition DOI: http://dx.doi.org/10.3329/jbau.v9i1.8742 JBAU 2011; 9(1): 43-47

scholarly journals Mycoflora Associated With Momordica Charantia L. And Their Pathogenic Potentiality

2016 ◽  
Vol 42 (2) ◽  
pp. 145-152
Author(s):  
Md Dulal Miya ◽  
Shamim Shamsi
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Flavus ◽  
Trichoderma Viride ◽  
Momordica Charantia ◽  
Bitter Gourd ◽  
Rhizopus Stolonifer ◽  
The World ◽  
Fusarium Sp

Momordica charantia L. (Bitter gourd) is one of the popular vegetable in Bangladesh as well as in the world. After harvesting the vegetables are contaminated with fungi within 3-4 days of short storage. An investigation was carried out to find out the fungi associated with fruits of two varieties of Momordica charantia during the tenure of May 2015 to June 2016. Nine species of fungi namely, Aspergillus flavus Link, A. fumigatus Fresenius, A. niger van Tiegh, Curvularia brchyspora Boedijn, Fusarium Link, Mucor Fresen, Penicillium Link, Rhizopus stolonifer (Ehrenb.:Fr.) Vuill and Trichoderma viride Pers. were found to be associated with the selected vegetable. .Aspergillus niger was predominating fungus associated with both the varieties of bitter gourd. Among the isolated 9 fungi A. niger, C. brchyspora, Fusarium sp., R. stolonifer and T. viride were found to be pathogenic to both the varieties of M. charantia. Asiat. Soc. Bangladesh, Sci. 42(2): 145-152, December 2016

scholarly journals THE POTENTIAL OF ENDOPHYTIC FUNGI AS BIOCONTROL AND PHOSPATE SOLUBILIZATION AGENT IN Capsicum anuum

2020 ◽  
Vol 3 (3) ◽  
pp. 16-19
Author(s):  
Dwi Ningsih Susilowati
Keyword(s):  
Growth Inhibition ◽  
Endophytic Fungi ◽  
Phytophthora Capsici ◽  
Pathogenic Fungi ◽  
Culture Method ◽  
Colletotrichum Acutatum ◽  
Limiting Factor ◽  
Dual Culture ◽  
Fusarium Sp ◽  
Index Value

The productivity of chili (Capsicum annuum) in Indonesia is currently very low. Some factors that influenced it including the presence of pathogenic microorganisms which lead to the low availability of phosphate in the soil. This condition become a limiting factor for plant growth and production. Endophytic fungi can be used as antagonistic agents in inhibiting pathogenic fungi and to increase the efficiency of phosphate solubilization known as phospholytic fungi. This study aimed to find antagonistic agents from endophytic fungi that can suppress the growth of pathogenic fungi and test the ability of endophytic fungi to dissolve phosphate. Fungi isolates used were BB-Biogen collection isolates, consisting of 42 endophytic fungi isolates, and 3 pathogenic fungi isolates (Fusarium sp., Colletotrichum acutatum, Phytophthora capsici) on chili plants (C. annuum). The antagonism test was carried out using the dual culture method in the Potato Dextrose Agar (PDA) medium for 5 days incubation at temperature (± 28 ° C). The parameters measured were based on the formation of inhibition zones and the calculation of the percentage of growth inhibition of fungi isolates. The test results obtained 7 representative fungi isolates (RIVA4, RIVA5, MIVD2, Aspergillus niger, Cladosporium sp., Cladosporium oxysporum, and Chaetomium globosum). Based on the calculation of the percentage of growth inhibition, fungi isolates with RIVA5 code have a higher potential in inhibiting the growth of all three pathogenic fungi. Calculation of the percentage of endophytic fungi inhibition of RIVA5 were 70.3% (Fusarium sp.), 63.3% (C. acutatum), and 60% (P. capsici). Phosphate test was carried out by the cork borer method in pikovskaya medium for 4 days incubation at 27-28 ° C. The parameters measured were based on the formation of clear zones around the colony. There were 4 endophytic fungi isolates (MIVA4, MIVF7, Aspergillus sydowii, and  A. niger) formed a clear zone around the colony, which indicates the presence of phospholytic activity. Based on the calculation of the phospholytic index, A. niger isolates have a high phosphate solubility index value of 5.

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