scholarly journals Efficacy of some plant extracts on the mycelial growth of Colletotrichum gloeosporioides

1970 ◽  
Vol 9 (1) ◽  
pp. 43-48 ◽  
Author(s):  
A Mukherjee ◽  
S Khandker ◽  
MR Islam ◽  
Sonia B Shahid
Keyword(s):  
Growth Inhibition ◽  
Plant Extracts ◽  
Growth And Development ◽  
Radial Growth ◽  
Mycelial Growth ◽  
Colletotrichum Gloeosporioides ◽  
Causal Agent ◽  
Garlic Extract ◽  
Tobacco Leaf ◽  
Anti Fungal

The anti fungal effects of some plants extracts namely tobacco leaf, keora seed, keora, mahogoni, gaint indian milky weed, garlic and ginger at different concentrations (30%, 40%, 50%, 60% and 70%) on the growth and development of C. gloeosporioides, causal agent of anthracnose of mango were evaluated. Radial growth of C. gloeosporioides was recorded. The growth inhibition increase with the increase of concentration of all the plant extracts. Highest mycelial growth inhibition (74.35%) was observed in case of garlic extracts at 70% concentration. Garlic extract at 50% and 60% concentration were also effective than other treatments.Keywords: C. Gloeosporiodes; Plant extracts; Inhibition DOI: http://dx.doi.org/10.3329/jbau.v9i1.8742 JBAU 2011; 9(1): 43-47

scholarly journals In Vitro Screening of Fungicides and Plant Extracts Against Colletotrichum Gloeosporioides (Penz.) Sacc. the Causal Agent of Anthracnose Disease of Rauwolfia Serpentina (L.) Benth Ex Kurz

2019 ◽  
Vol 45 (1) ◽  
pp. 35-43
Author(s):  
Zuhra Yasmin ◽  
Shamim Shamsi
Keyword(s):  
Growth Inhibition ◽  
Plant Extracts ◽  
Azadirachta Indica ◽  
Moringa Oleifera ◽  
Colletotrichum Gloeosporioides ◽  
Psidium Guajava ◽  
Causal Agent ◽  
Citrus Limon ◽  
Anthracnose Disease ◽  
Rauwolfia Serpentina

Ten fungicides viz., Amcogin 50 WP, Autostin 50 WDG, Capvit 77 WP, Dithane M 45, Greengel 72 WP, Haybit 80 WG, Oxivit 40 SC, Ridomil Gold MZ, Sulcox 50 WP and Tilt 250 EC at 100, 200, 300, 400 and 500 ppm concentrations were tested against, Colletotrichum gloeosporioides (Penz.) Sacc., the causal agent of anthracnose disease of Rauwolfia serpentina (L.) Benth ex Kurz following poisoned food technique. Amcogin, Autostin and Tilt showed complete growth inhibition of C. gloeosporioides at 100 ppm concentration. Ethanol extracts of ten plants viz., Adhatoda vesica, Azadirachta indica, Citrus limon, Curcuma longa, Lantana camara, Moringa oleifera, Ocimum sanctum, Psidium guajava, Thuja orientalis and Vitex negundo were selected to evaluate their efficacy at 5, 10, 15 and 20% concentrations against the same pathogenic fungus. Out of the ten plant extracts, Azadirachta indica, Citrus limon, Moringa oleifera and Psidium guajava showed complete radial growth inhibition of C. gloeosporioides at 20% concentration. Amcogin, Autostin, and Tilt are suggested as best inhibiting chemical fungicides for C. gloeosporioides. Extracts of A. indica, C. limon, M. oleifera and Psidium guajava were also found to be superior to other plant extracts tested in controlling the test pathogen. Asiat. Soc. Bangladesh, Sci. 45(1): 35-43, June 2019

scholarly journals Bio-efficacy of phytoextracts and oil cakes on Macrophomina phaseolina (Tassi) causing stem rot disease of jute, Corchorus spp.

2014 ◽  
Vol 6 (2) ◽  
pp. 530-533 ◽  
Author(s):  
P. N. Meena ◽  
A. N. Tripathi ◽  
B. S. Gotyal ◽  
S. Satpathy
Keyword(s):  
Growth Inhibition ◽  
Plant Extracts ◽  
Mycelial Growth ◽  
Macrophomina Phaseolina ◽  
Stem Rot ◽  
Lawsonia Inermis ◽  
Leaf Extracts ◽  
Neem Cake ◽  
Oil Cakes ◽  
Rot Disease

In the present study efforts were made to explore the efficacy of various plant extracts and oil cakes against Macrophomina phaseolina. The efficacy of eight different plant species, Zingiber officianale, Aloe indica, Lawsonia inermis, Chenopodium album, Allium cepa, Piper betel, Murraya koenigii, Parthenium hysterophorus aqueous extracts including oil cakes of mustard and neem were evaluated by using of poisoned food technique in vitro for their inhibitory effect on mycelial growth of M. phaseolina causing stem rot of jute (Corchorus olitorius and C. capsularis). The rhizome extract of Z. officinale produced maximum growth inhibition (74.59%) of the pathogen followed by leaf extracts of A. indica (63.57%), and leaf extract of L. inermis (60.17%) at the concentration of 10%. The maximum mycelial growth inhibition (52.40%) was recorded with neem cake (Azadirachta indica) at the concentrations of 20% followed by 42.61% and 29.60% with concentration of 15% and 10%, respectively. However, maximum mycelial growth inhibition (19.42%) was recorded with mustard cake (Brassica juncea) at the concentration of 20% followed by 16.64% and 12.20% at the concentration of 15% and 10% respectively. In general mycelial growth inhibition was dose dependent and it was maximum in case of neem cake than mustard cake. The present study revealed that, these plant extracts and oilcake extracts could be exploited for the possible control of deadly pathogen M. phaseolina. Accordingly, this is an important proactive measure in preventing the spread of the stem rot disease through a more ecofriendly approach.

scholarly journals In Vitro Screening of Fungicides and Plant Extracts Against Pathogenic Fungi Associated With Infected Fruits of Carica Papaya L

2018 ◽  
Vol 42 (2) ◽  
pp. 121-128
Author(s):  
M Rezuana Binte Helal ◽  
Shamim Shamsi
Keyword(s):  
Growth Inhibition ◽  
Plant Extracts ◽  
Radial Growth ◽  
Carica Papaya ◽  
Curcuma Longa ◽  
Pathogenic Fungi ◽  
Ocimum Sanctum ◽  
Fusarium Sp ◽  
Thuja Orientalis

Five fungicides viz., Autostin 50 WDG, Dithane M 45, Greengel 72 WP, Ridomil Gold MZ 68 WG   and Tilt 250 EC exclusively were tested against three pathogenic fungi viz., Colletotrichum gloeosporioides (Penz.) Sacc., Fusarium nivale (Fr.) Ces. and Fusarium sp. associated with Carica papaya L. following poisoned food technique. At 100 ppm  Autostin showed complete growth inhibition of C. gloeosporioides, F. nivale and Fusarium sp., while  Tilt 250 EC showed complete growth inhibition of C. gloeosporioides at the same concentration. These fungicides at 400 and 500 ppm showed complete growth inhibition of F. nivale. Tilt 250 EC showed complete growth inhibition of Fusarium sp. at 300, 400 and 500 ppm. Eight plants extract, viz., Adhatoda vasica, Azadirachta indica, Curcuma longa, Ocimum sanctum, Lantana camara, Tagetes erecta, Thuja orientalis and Vitex negundo were also tested for their  efficacy at 5, 10, 15 and 20% concentrations against these pathogenic fungi. Out of the eight plant extracts, Ocimum sanctum showed highest (88.57%) radial growth inhibition of C. gloeosporioides at 20% concentration. Curcuma longa showed highest (88.55%) radial growth inhibition of F. nivale and Adhatoda vesica showed highest (80.02%) radial growth inhibition of Fusarium sp. at the same concentrations. The present investigation suggests Autostin 50 WDG and Tilt 250 EC as best inhibiting chemical fungicides for C. gloeosporioides, F. nivale and Fusarium sp. Extracts of O. sanctum, C. longa and Adhatoda vesica were found to be superior to other plant extracts tested. Journal of Bangladesh Academy of Sciences, Vol. 42, No. 2, 121-128, 2018

scholarly journals In Vitro Evaluation of Selected Plant extracts and Chemicals against Pathogenic Fungi isolated from Momordica Charantia L.

2017 ◽  
Vol 41 (1) ◽  
pp. 11-16
Author(s):  
Md Dulal Miya ◽  
Shamim Shamsi
Keyword(s):  
Sodium Chloride ◽  
Sodium Bicarbonate ◽  
Growth Inhibition ◽  
Plant Extracts ◽  
Radial Growth ◽  
Pathogenic Fungi ◽  
Momordica Charantia ◽  
Rhizopus Stolonifer ◽  
Fresh Vegetables ◽  
Fusarium Sp

Five plant extracts and two chemicals were evaluated against radial growth of five pathogenic fungi isolated from fresh vegetables of two varieties of Momordica charantia L. The isolated fungi were Aspergillus niger Van Tiegh, Curvularia brachyspora Boedijn, Fusarium Link, Rhizopus stolonifer (Ehrenb.:Fr.) Vuill and Trichoderma viride Pers.. Five plant extracts namely Allium sativum L., Azadirachta indicia A. Juss., Citrus limon (L.) Burm. f, Mangifera indica L. and Psidium guajava L. were evaluated for inhibiting growth of above mentioned fungi associated with the vegetable. Out of the five plant extracts, A. sativum showed complete radial growth inhibition of A. niger, C. brachyspora, Fusarium sp. and R. stolonifer at 15 and 20% concentrations. Azadirachta indica showed complete radial growth inhibition of C. brachyspora and Fusarium sp. Rhizopus stolonifer was completely inhibited by 15 and 20% concentration owing to leaf extract of A. indica and P. guajava. The same fungus was completely inhibited by M. indica at 20% concentration. Fusarium sp. was completely inhibited by C. limon at 20% concentration. Two chemicals, sodium bicarbonate and sodium chloride were used against the test pathogens and sodium bicarbonate showed complete inhibition of radial growth of A. niger, C. brachyspora, Fusarium sp. and R. stolonifer at 20% concentration. The last one also inhibited by the chemical at 15% concentration. Sodium chloride showed highest 95% inhibition of radial growth of T. viride at 20% concentration.Journal of Bangladesh Academy of Sciences, Vol. 41, No. 1, 11-16, 2017

scholarly journals Antifungal activity of some botanical extracts on Fusarium oxysporum

2015 ◽  
Vol 10 (1) ◽  
Author(s):  
Domenico Rongai ◽  
Patrizio Pulcini ◽  
Barbara Pesce ◽  
Filomena Milano
Keyword(s):  
Antifungal Activity ◽  
Growth Inhibition ◽  
Fusarium Oxysporum ◽  
Plant Extracts ◽  
Mycelial Growth ◽  
Titratable Acidity ◽  
Punica Granatum ◽  
Inhibitory Effect ◽  
Total Phenolic ◽  
Conidia Germination

AbstractThe present paper describes the antifungal activity of some plant extracts on the development of Fusarium oxysporum f.sp. lycopersici. The best extracts were selected to be tested as a phytofungicide to control crop diseases, with the ultimate goal of developing a green alternative to synthetic fungicides. Using the conidia germination assay, of the 24 plant extracts tested, 15 reduced conidia germination and 6 completely inhibited germination. Extracts of Rivina humulis, Brassica carinata, Brunfelsia calyicina, Salvia guaranitica and Punica granatum showed good antifungal activity. The relationship between total phenolic content (TPC) in each plant extract tested and the percentage of mycelial growth inhibition showed a significant correlation (R2 = 0.69), while no correlation was found between total flavonoid content (TFC) and percentage mycelial growth inhibition. Among all extracts tested, Punica granatum and Salvia guaranitica showed the best inhibitory effect against Fusarium oxysporum f.sp. lycopersici . Our results indicate that plant extracts with a good antifungal activity generally had a high level of total polyphenolic content and titratable acidity, and low values of pH.

scholarly journals In vitro evaluation of antibiotic performances on Trichoderma harzianum and some crop infecting fungi

2016 ◽  
Vol 3 (3) ◽  
pp. 267 ◽  
Author(s):  
Md. Moshiur Rahman Akonda ◽  
Raihan Mujib Himel ◽  
Mohammad Ali ◽  
Md. Syeful Islam
Keyword(s):  
Growth Inhibition ◽  
Trichoderma Harzianum ◽  
Growth And Development ◽  
Colletotrichum Gloeosporioides ◽  
Inhibitory Effects ◽  
Tea Plantation ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Three Samples

An in vitro experiment was carried out to evaluate the effectiveness of antibiotics at different concentrations on growth and development of Trichoderma harzianum, Phytophthora infestans, Colletotrichum gloeosporioides, Corticium theae and Fusarium oxysporum found in tea plantation. Three samples viz. sample-1 (Validamycin 60% w/w) @ 45, 60 and 75 ppm, sample-2 (Hexaconazole 2.5% w/w+Validamycin 8.5% w/w) @ 55, 82.5 and 110 ppm and sample-3 (Streptomycin 9% w/w+Tetracyclin hydrochloride 1% w/w) @ 50, 75 and 100 ppm were tested. The result showed that Antibiotics have inhibitory effects on T. harzianum. Unsatisfactory performances in terms of per cent growth inhibition (<80) were recorded on crop infecting fungi. C. theae treated with sample-1 @ 75 ppm and C. gloeosporioides with sample-2 @ 110 ppm had shown maximum 25.50 and 54.19 per cent growth inhibition, respectively. The highest 70.53 per cent growth inhibition of C. theae was observed in sample-3 treated @ 100 ppm. Considering the findings it can be recommended not to use above antibiotics with their respective concentrations in plant agriculture for controlling diseases caused by the said fungi.

scholarly journals Inhibitory Potential of Selected Fungicides and Plant Extracts Against Mycelial Growth of Fusarium oxysporum f. sp. ciceri Causal Agent of Chickpea Wilt

2021 ◽  
Vol 1 (1) ◽  
pp. 09-13
Author(s):  
Hafiz Abdul Haseeb ◽  
Usama Naseem ◽  
Safdar Ali ◽  
Shahbaz Talib Sahi
Keyword(s):  
Fusarium Oxysporum ◽  
Plant Extracts ◽  
Mycelial Growth ◽  
Colony Growth ◽  
Causal Agent ◽  
Limiting Factors ◽  
Chickpea Wilt ◽  
Inhibitory Potential

Chickpea wilt is one of the major limiting factors, for low yield of chickpea. In Pakistan chickpea wilt causes 10-50% losses every year. At all concentrations, Carbendazim and Benomyl proved most effective while Acrobat was least effective in suppressing the mycilial growth of the Fusarium oxysporum f. sp. ciceri.  However, higher concentration of Acrobat was also slightly effective. Among the plant extracts, higher concentrations of Sufaida and Neem proved to be effective while onion failed to control the colony growth of Fusarium oxysporum f.sp. ciceri at all concentrations.

Screening on Antagonistic Actinomycetes of Pepper Anthracnose

2013 ◽  
Vol 864-867 ◽  
pp. 2509-2513
Author(s):  
Gui Fang Jiang ◽  
Li Song
Keyword(s):  
Antimicrobial Activity ◽  
Growth Inhibition ◽  
Mycelial Growth ◽  
Colletotrichum Gloeosporioides ◽  
Separation Method ◽  
Dilution Plate ◽  
Secondary Screening ◽  
Plate Separation ◽  
Antagonistic Actinomycetes

The 87 strains actinomycetes were separated and purificated from soil by dilution plate separation method, with pepper anthracnose (Colletotrichum gloeosporioides) as indicator pathogens, these actinomycetes had primary and secondary screening screened by cross method and mycelial growth inhibition method respectively, finally, three actinomycetes had obvious antimicrobial activity separated.

Colletotrichum Gloeosporioides Species Complex: Pathogen Causing Anthracnose, Gummosis and Die-Back Diseases of Cashew (Anacardium Occidentale L.) In Ghana

2020 ◽  
Vol 2 (6) ◽  
Author(s):  
A. Muntala ◽  
P. M. Norshie ◽  
K. G. Santo ◽  
C. K. S. Saba
Keyword(s):  
Species Complex ◽  
Colletotrichum Gloeosporioides ◽  
Potato Dextrose Agar ◽  
Causal Agent ◽  
Anacardium Occidentale ◽  
Causative Organism ◽  
Pathogenicity Test ◽  
Small Water ◽  
Colletotrichum Gloeosporioides Species Complex

A survey was conducted in twenty-five cashew (Anacardium occidentale) orchards in five communities in the Dormaa-Central Municipality of Bono Region of Ghana to assess the incidence and severity of anthracnose, gummosis and die-back diseases on cashew. Cashew diseased samples of leaves, stem, inflorescences, twigs, flowers, nuts and apples showing symptoms (e. g. small, water-soaked, circular or irregular yellow, dark or brown spots or lesions on leaves, fruits and flowers, sunken surface, especially on the apples, blight, gum exudates) were collected for isolation of presumptive causative organism. The pathogen was isolated after disinfecting the excised diseased pieces in 70% ethanol, plated on potato dextrose agar (PDA) and incubated at 28 oC for 3 to 7 days. The identity of the putative pathogen was morphologically and culturally confirmed as belonging to Colletotrichum gloeosporioides species complex using standard mycological identification protocols. The pathogen had varied conidia sizes of between 9-15 up to 20 μm in length and diameter of 3-6 μm. The conidia were straight and cylindrically shaped with rounded or obtuse ends. The septate mycelium was whitish-grey, velvety and cotton-like in appearance from the top. The results confirmed the presence of the pathogen in the orchards with incidence ranging from 6.9% and 14.0% for gummosis and averaged 22.9% for anthracnose infected orchards. The result of the pathogenicity test confirmed the isolates to be pathogenic on inoculated cashew seedlings and were consistently re-isolated, thereby establishing the pathogen as the true causal agent of the said diseases in cashew trees and thus completed the Koch’s postulate.

Management of collar rot disease in chickpea by Trichoderma species

2020 ◽  
Vol 7 (03) ◽  
Author(s):  
PREM PANDEY ◽  
G. C. SAGAR ◽  
SUNDARMAN SHRESTHA2 ◽  
HIRAKAJI MANANDHAR ◽  
RITESH K. YADAV ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Mycelial Growth ◽  
Sclerotium Rolfsii ◽  
Maximum Growth ◽  
Pot Experiment ◽  
Trichoderma Spp ◽  
Trichoderma Species ◽  
Collar Rot ◽  
Rot Disease

Nine isolates of Trichoderma spp. were isolated from different agro- ecological regions of Nepal viz; Jumla, Palpa, Chitwan, Tarahara, Banke, Illam and Salyan and screened against Sclerotium rolfsii Sacc. Adreded soil borne phytopathogen causing collar rot of chickpea in chickpea; In-vitro efficacy of nine fungal antagonist (Trichoderma spp.) against Sclerotium rolfsii were screened. Pot experiment was done to find out the effective management of S. rolfsi through Tricoderma using different methods i.e. Seed treatment, soil drenching and soil application. All the tested isolates of Trichoderma spp. were found effective on mycelial growth inhibition and sclerotial parasitization of S. rolfsii. Trichoderma isolated from Palpa district showed maximum growth inhibition (%) of pathogen periodically after 48(93.78%), 72(96.00%), 96(97.96%) and 120(100.00%) hours of inoculation. Parasitized sclerotium showed minimum sclerotial germination on agar plates. Moreover, Trichoderma species isolated from Palpa districts showed second best percent mycelial growth inhibition periodically at 72(25.00%), 120(29.16%), 168(29.16%) and 216(29.16%).In pot experiment at 40 days after sowing, Seedling height was maximum in soil drenching with 30g per 100ml of water (22.27cm) and Mortality percentage of seedlings was least or highest disease control was observed in seed treated with 109cfu/ml (0.000%).

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