In-vitro Studies of Plant Extracts against Alternaria brassicae (berk.) Sacc. of Indian Mustard (Brassica juncea (L.) Czern. and coss)

2016 ◽  
Vol 3 (1) ◽  
Author(s):  
MAHESH SINGH ◽  
H. K. SINGH
Keyword(s):  
Growth Inhibition ◽  
Plant Extract ◽  
Plant Extracts ◽  
Indian Mustard ◽  
Pathogenic Fungi ◽  
Alternaria Brassicae ◽  
Test Fungus ◽  
Poison Food Technique ◽  
Percent Growth Inhibition

Six plant extract viz., Datura (Daturastramonium), Eucalyptus (Eucalyptus globulous), Karang (Pongamiaglabra),Neem (Azadirachtaindica), Madar (Calotropis gigantean) and Wild sage (Lantana camara) were evaluated in-vitro by poison food technique @ 3, 6, 9 and 12% concentrations against Alternariabrassicaecausing blight of mustard. The results revealed that all the plant extracts inhibited the percent growth inhibition against test fungus as compared to control. However A.indica was found most efficacious with growth inhibition of (38.7%) followed by E. globulus (36.7%). Some extract such as P. glabra and D. stramonium showed moderate inhibition over control (30.9 and 21.6 respectively). Among all plant extract, C. gigantean showed least (15.3%) inhibition over control against A. brassicae. These plant extracts can possibly be used as management of seedborne pathogenic fungi in an eco-friendly way.

scholarly journals In Vitro Screening of Fungicides and Plant Extracts Against Pathogenic Fungi Associated With Infected Fruits of Carica Papaya L

2018 ◽  
Vol 42 (2) ◽  
pp. 121-128
Author(s):  
M Rezuana Binte Helal ◽  
Shamim Shamsi
Keyword(s):  
Growth Inhibition ◽  
Plant Extracts ◽  
Radial Growth ◽  
Carica Papaya ◽  
Curcuma Longa ◽  
Pathogenic Fungi ◽  
Ocimum Sanctum ◽  
Fusarium Sp ◽  
Thuja Orientalis

Five fungicides viz., Autostin 50 WDG, Dithane M 45, Greengel 72 WP, Ridomil Gold MZ 68 WG   and Tilt 250 EC exclusively were tested against three pathogenic fungi viz., Colletotrichum gloeosporioides (Penz.) Sacc., Fusarium nivale (Fr.) Ces. and Fusarium sp. associated with Carica papaya L. following poisoned food technique. At 100 ppm  Autostin showed complete growth inhibition of C. gloeosporioides, F. nivale and Fusarium sp., while  Tilt 250 EC showed complete growth inhibition of C. gloeosporioides at the same concentration. These fungicides at 400 and 500 ppm showed complete growth inhibition of F. nivale. Tilt 250 EC showed complete growth inhibition of Fusarium sp. at 300, 400 and 500 ppm. Eight plants extract, viz., Adhatoda vasica, Azadirachta indica, Curcuma longa, Ocimum sanctum, Lantana camara, Tagetes erecta, Thuja orientalis and Vitex negundo were also tested for their  efficacy at 5, 10, 15 and 20% concentrations against these pathogenic fungi. Out of the eight plant extracts, Ocimum sanctum showed highest (88.57%) radial growth inhibition of C. gloeosporioides at 20% concentration. Curcuma longa showed highest (88.55%) radial growth inhibition of F. nivale and Adhatoda vesica showed highest (80.02%) radial growth inhibition of Fusarium sp. at the same concentrations. The present investigation suggests Autostin 50 WDG and Tilt 250 EC as best inhibiting chemical fungicides for C. gloeosporioides, F. nivale and Fusarium sp. Extracts of O. sanctum, C. longa and Adhatoda vesica were found to be superior to other plant extracts tested. Journal of Bangladesh Academy of Sciences, Vol. 42, No. 2, 121-128, 2018

Antimicrobial effects of plant extracts against Clostridium perfringens with respect to food-relevant influencing factors

2021 ◽  
Author(s):  
Ulrike Friedlein ◽  
Samart Dorn-In ◽  
Karin Schwaiger
Keyword(s):  
Influencing Factors ◽  
Plant Extract ◽  
Plant Extracts ◽  
Pathogenic Bacteria ◽  
Screening Method ◽  
Extrinsic Factors ◽  
Antimicrobial Effects ◽  
Extract Concentration ◽  
Microdilution Method

The application of plant extracts (PEs) could be a promising option to satisfy consumers’ demand for natural additives to inhibit growth of variable pathogenic bacteria. Thus, the aim of this study was to develop a standardized microdilution method to examine the antimicrobial effects of ten hydrophilic plant extracts against two strains of C. perfringens facing various food-relevant influencing factors. Due to the high opacity of PEs, resazurin was used as an indicator for bacterial growth instead of pellet formation. The highest value of the minimum inhibitory concentration (MIC) of the replications of each PE was defined as effective plant extract concentration (EPC), whereas the next concentration beneath the lowest MIC value was defined as the ineffective plant extract concentration (IEPC). The EPC of seven PEs: allspice, cardamom, cinnamon, clove, coriander, ginger and mace were between 0.625 - 10 g/kg, whereas extracts of caravey, nutmeg and thyme showed no antimicrobial activity up to the maximum concentration tested (10 g/kg) against C. perfringens in vitro. Two intrinsic factors, sodium chloride and sodium nitrite, displayed either synergistic/additive effects or no interaction with most PEs. By combination with PEs at its ineffective plant concentration (IEPC, 0.08 – 1.25 g/kg), MIC of NaCl and NaNO2 decreased from 25 – 50 g/kg to 6 – 25 g/kg and > 200 mg/kg to 0.2 – 100 mg/kg respectively. On the contrary, lipid (sun flower oil) at a low concentration inhibited the antimicrobial effects of all tested PEs. For extrinsic factors, only allspice, ginger and coriander could maintain their antimicrobial effects after being heated to 78 °C for 30 min. The synergistic effect between PEs and pH values (5.0 and 5.5) was also found for all PEs. The established screening method with resazurin and defining EPC and IEPC values allows the verification of antimicrobial effects of PEs under various food-relevant influencing factors in a fast and reproducible way.

scholarly journals In vitro evaluation of fungicides and some plant extracts against rice sheath rot pathogen Sarocladium oryzae

2016 ◽  
Vol 29 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Shamim Shamsi ◽  
Pranami Chowdhury
Keyword(s):  
Plant Extracts ◽  
Radial Growth ◽  
Mangifera Indica ◽  
Ethanol Extract ◽  
Tagetes Erecta ◽  
Asparagus Racemosus ◽  
Test Fungus ◽  
Datura Metel ◽  
Sheath Rot

Sarocladium oryzae (Sawada) W. Gams & D. Hawksworth, the causal agent of sheath rot of rice was isolated from sheath rot infected rice sheaths and grains. Among ten tested fungicides Tall 25 EC completely inhibited radial growth of the fungus even at the lowest concentration 100 ppm. Similarly ethanol leaf extract of ten plants, namely Allium sativum, Artocarpus heterophyllus, Asparagus racemosus, Azadirachta indica, Citrus medica, Datura metel, Mangifera indica, Nerium indicum, Senna alata and Tagetes erecta at 5, 10 and 20% concentrations were screened for their fungicidal activity against the test fungus. All the plant extracts completely inhibited the radial growth of the test fungus at 20% concentration except Asparagus racemosus and C. medica. Ethanol extract of Tagetes erecta and Mangifera indica also completely inhibited the radial growth of the test fungus at 10% concentration.Bangladesh J. Sci. Res. 29(1): 47-54, June-2016

scholarly journals In vitro activity of plant extracts against some important plant pathogenic fungi of tomato

2017 ◽  
Vol 11 (06) ◽  
pp. 683-689 ◽  
Author(s):  
James W. Muthomi ◽  
◽  
Geraldin M. W. Lengai ◽  
Maina J. Wagacha ◽  
Rama D. Narla ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Pathogenic Fungi ◽  
Vitro Activity ◽  
Plant Pathogenic Fungi ◽  
In Vitro Activity

scholarly journals In-vitro evaluation of six bio-product against coriander stem gall disease

2020 ◽  
Vol 35 (1-2) ◽  
Author(s):  
Munish Leharwan ◽  
Meenu Gupta ◽  
Sanjeev Leharwan
Keyword(s):  
Growth Inhibition ◽  
Mycelial Growth ◽  
Significant Inhibition ◽  
Fresh Milk ◽  
Cent Concentration ◽  
Cow Urine ◽  
Stem Gall ◽  
Butter Milk ◽  
Poison Food Technique

Stem gall of coriander caused by Protomyces macrosporus Unger. is a highly devastating seed and soil borne pathogen in coriander and has emerged as one of the major disease causing substantial quantitative and qualitative losses. In the present studies, efficacy of six bio-products, namely, Neemazal, cow urine, butter milk, fresh milk, beejamrit and jeevamrit was screened against mycelial growth inhibition of P. macrosporus under in vitro conditions. Extracts of these bio-products were evaluated at three different concentrations (i.e 10, 20 and 30%) by poison food technique. Results revealed that that among the various bio-products evaluated against test pathogen, irrespective of concentration tested, all the bio-products resulted in significant inhibition in mycelial growth of test pathogen with neemazal giving the complete inhibition [100.00 % (90.00)] followed by fresh milk [30.66 % (28.44)] and butter milk [26.55 % (25.99)], though these treatments were statistically different from one another. Different concentrations (10 to 30 %) of bio-products, irrespective of the treatments showed that 30 per cent concentration of all bio-products was most efficacious in inhibiting the mycelial growth of the test pathogen giving 69.95 per cent (36.77) growth inhibition. This was followed 20 and 10 per cent concentration of bio-products resulting in 55.58 per cent (32.49) and 45.53 per cent (16.30) growth inhibition, respectively. The result regarding evaluation of bio-products under in vitro conditions against the P. macrosporus showed that Neemazal treatment was most effective at all concentrations and completely inhibited the test fungus followed by 30 per cent fresh milk resulting in the mycelial growth inhibition of 51.60 per cent.

scholarly journals In Vitro Evaluation of Effect of Different Essential Oils in Management of Post-Harvest Fruit Rot of Banana (Musa Paradisiaca) Caused by Colletotrichum spp

2021 ◽  
Vol 9 (3) ◽  
pp. 187-192
Author(s):  
Pramod Gairhe ◽  
Sandesh Bhandari ◽  
Hom Prasad Sitaula ◽  
Beautina Karki ◽  
Hira Kaji Manandhar
Keyword(s):  
Growth Inhibition ◽  
Radial Growth ◽  
Coconut Oil ◽  
Fruit Rot ◽  
Mustard Oil ◽  
Cinnamon Oil ◽  
Post Harvest ◽  
And Control ◽  
Percent Growth Inhibition

A study was conducted to evaluate the in vitro efficacy of different essential oils in the management of post-harvest fruit rot of banana. It was carried out in completely randomized design (CRD) with three replications and six treatments at Nepal Plant Diseases and Agro Associates (NPDA). The each of six treatments: cinnamon oil (Cinnamomum verum), mustard oil (Brassica oleracea), castor oil (Ricinus communis), neem oil (Azadirachta indica), coconut oil (Cocos nucifera) and control were used at concentrations 200ppm, 500ppm and 1000ppm respectively. The radial growth of mycelium (mm) and percent growth inhibition (%) of Colletotrichum spp. was recorded. The decrease in the radial growth and increase in the percent growth inhibition was found in all the treatments except control as their concentration was increased, in which the lowest radial growth and the highest percent growth inhibition was found at 1000 ppm concentration. At 1000 ppm concentration, cinnamon oil shows the lowest radial growth and the highest percent growth inhibition (1.67mm and 98.15%) followed by mustard oil (54.00mm and 40.00%), neem oil (55.17mm and 38.70%), castor oil (55.83mm and 37.96%), coconut oil (61.17mm and 32.04%) and control (90mm and 0.00%) respectively.Thus, cinnamon oil is considered as a good essential oil in regards to a management of post-harvest disease of banana. Int. J. Appl. Sci. Biotechnol. Vol 9(3): 187-192.    

scholarly journals In vitro screening of different Pseudomonas fluorescens isolates to study lytic enzyme production and growth inhibition during antagonism of Fusarium oxysporum f. sp. cumini, wilt causing pathogen of cumin

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Ridhdhi Rathore ◽  
Dinesh N. Vakharia ◽  
Dheeraj Singh Rathore
Keyword(s):  
Growth Inhibition ◽  
Fusarium Oxysporum ◽  
Pseudomonas Fluorescens ◽  
Close Association ◽  
Hydrolytic Enzymes ◽  
Lytic Enzyme ◽  
Cell Wall Degrading Enzymes ◽  
Causal Pathogen ◽  
Percent Growth Inhibition

Abstract Land plants exist in close association with bacterial and fungal microbes, where some associations can be pathogenic and others can be mutualistic/beneficial. One such relation exists between host plant, Cuminum cyminum L. (Cumin) and Fusarium oxysporum f. sp. cumini (Foc), the causal pathogen of cumin wilt and Pseudomonas fluorescens (Pf), where Pf acts as a bio-agent for inhibiting Foc and promoting plant growth of cumin. In this study, antagonism by 10 different Pf isolates against Foc was studied under laboratory conditions through percent growth inhibition and biochemical mechanisms. Among these Pf isolates, Pf-5 exhibited the highest in vitro growth inhibition (82.51%). A positive correlation was observed between percent growth inhibition and specific activities of hydrolytic enzymes, chitinase, β-1, 3 glucanase, and protease, where a negative correlation was observed with cell wall degrading enzymes, cellulase and polygalacturonase. To conclude, isolate Pf-5 could be a potential biocontrol agent for Fusarium wilt disease of cumin.

Sign in / Sign up

Export Citation Format

Share Document