scholarly journals In Vitro Screening of Fungicides and Plant Extracts Against Pathogenic Fungi Associated With Infected Fruits of Carica Papaya L

2018 ◽  
Vol 42 (2) ◽  
pp. 121-128
Author(s):  
M Rezuana Binte Helal ◽  
Shamim Shamsi
Keyword(s):  
Growth Inhibition ◽  
Plant Extracts ◽  
Radial Growth ◽  
Carica Papaya ◽  
Curcuma Longa ◽  
Pathogenic Fungi ◽  
Ocimum Sanctum ◽  
Fusarium Sp ◽  
Thuja Orientalis

Five fungicides viz., Autostin 50 WDG, Dithane M 45, Greengel 72 WP, Ridomil Gold MZ 68 WG   and Tilt 250 EC exclusively were tested against three pathogenic fungi viz., Colletotrichum gloeosporioides (Penz.) Sacc., Fusarium nivale (Fr.) Ces. and Fusarium sp. associated with Carica papaya L. following poisoned food technique. At 100 ppm  Autostin showed complete growth inhibition of C. gloeosporioides, F. nivale and Fusarium sp., while  Tilt 250 EC showed complete growth inhibition of C. gloeosporioides at the same concentration. These fungicides at 400 and 500 ppm showed complete growth inhibition of F. nivale. Tilt 250 EC showed complete growth inhibition of Fusarium sp. at 300, 400 and 500 ppm. Eight plants extract, viz., Adhatoda vasica, Azadirachta indica, Curcuma longa, Ocimum sanctum, Lantana camara, Tagetes erecta, Thuja orientalis and Vitex negundo were also tested for their  efficacy at 5, 10, 15 and 20% concentrations against these pathogenic fungi. Out of the eight plant extracts, Ocimum sanctum showed highest (88.57%) radial growth inhibition of C. gloeosporioides at 20% concentration. Curcuma longa showed highest (88.55%) radial growth inhibition of F. nivale and Adhatoda vesica showed highest (80.02%) radial growth inhibition of Fusarium sp. at the same concentrations. The present investigation suggests Autostin 50 WDG and Tilt 250 EC as best inhibiting chemical fungicides for C. gloeosporioides, F. nivale and Fusarium sp. Extracts of O. sanctum, C. longa and Adhatoda vesica were found to be superior to other plant extracts tested. Journal of Bangladesh Academy of Sciences, Vol. 42, No. 2, 121-128, 2018

scholarly journals In Vitro Evaluation of Selected Plant extracts and Chemicals against Pathogenic Fungi isolated from Momordica Charantia L.

2017 ◽  
Vol 41 (1) ◽  
pp. 11-16
Author(s):  
Md Dulal Miya ◽  
Shamim Shamsi
Keyword(s):  
Sodium Chloride ◽  
Sodium Bicarbonate ◽  
Growth Inhibition ◽  
Plant Extracts ◽  
Radial Growth ◽  
Pathogenic Fungi ◽  
Momordica Charantia ◽  
Rhizopus Stolonifer ◽  
Fresh Vegetables ◽  
Fusarium Sp

Five plant extracts and two chemicals were evaluated against radial growth of five pathogenic fungi isolated from fresh vegetables of two varieties of Momordica charantia L. The isolated fungi were Aspergillus niger Van Tiegh, Curvularia brachyspora Boedijn, Fusarium Link, Rhizopus stolonifer (Ehrenb.:Fr.) Vuill and Trichoderma viride Pers.. Five plant extracts namely Allium sativum L., Azadirachta indicia A. Juss., Citrus limon (L.) Burm. f, Mangifera indica L. and Psidium guajava L. were evaluated for inhibiting growth of above mentioned fungi associated with the vegetable. Out of the five plant extracts, A. sativum showed complete radial growth inhibition of A. niger, C. brachyspora, Fusarium sp. and R. stolonifer at 15 and 20% concentrations. Azadirachta indica showed complete radial growth inhibition of C. brachyspora and Fusarium sp. Rhizopus stolonifer was completely inhibited by 15 and 20% concentration owing to leaf extract of A. indica and P. guajava. The same fungus was completely inhibited by M. indica at 20% concentration. Fusarium sp. was completely inhibited by C. limon at 20% concentration. Two chemicals, sodium bicarbonate and sodium chloride were used against the test pathogens and sodium bicarbonate showed complete inhibition of radial growth of A. niger, C. brachyspora, Fusarium sp. and R. stolonifer at 20% concentration. The last one also inhibited by the chemical at 15% concentration. Sodium chloride showed highest 95% inhibition of radial growth of T. viride at 20% concentration.Journal of Bangladesh Academy of Sciences, Vol. 41, No. 1, 11-16, 2017

scholarly journals Antagonistic potentials of some soil fungi against three post‐harvest pathogenic fungi of Carica papaya L.

2019 ◽  
Vol 28 (1) ◽  
pp. 1-7
Author(s):  
Rezuana Binte Helal ◽  
Shamim Shamsi
Keyword(s):  
Growth Inhibition ◽  
Radial Growth ◽  
Soil Fungi ◽  
Carica Papaya ◽  
Trichoderma Viride ◽  
Pathogenic Fungi ◽  
Dual Culture ◽  
Volatile Metabolites ◽  
Maximum Inhibition ◽  
Fusarium Sp

Four antagonistic fungi were isolated from the papaya field soil by serial dilution and were identified as Aspergillus flavus Link., A. fumigatus Fresenius, A. niger van Tiegh. and Trichoderma viride Pers. The soil fungi were selected to evaluate their antagonistic potentiality against the three postharvest pathogenic fungi of Carica papaya L. following "dual culture colony interaction” and volatile and non-volatile metabolites. In dual culture colony interaction, out of four soil fungi, T. viride showed the highest growth inhibition on C. gloeosporioides (84.28%), F. nivale (95.23%) and Fusarium sp. (87.15%). On the other hand A. niger showed the highest growth inhibition on C. gloeosporioides (77.39%), F. nivale (98.63%) and Fusarium sp. (35.05%). The maximum inhibition of radial growth of C. gloeosporioides (77.64%), F. nivale (58.76%) and Fusarium sp. (79.37%). were observed in case of T. viride owing to the volatile metabolites. Whereas the maximum inhibition of radial growth of C. gloeosporioides (58.23%), F. nivale (37.43%) and Fusarium sp. (82.31%) were observed in case of A. niger owing to the volatile metabolites. The maximum inhibition of radial growth of C. gloeosporioides (90.90%), F. nivale (89.13%) and Fusarium sp. (76.84%) were observed in case of T. viride owing to the effect of non-volatile metabolites. The maximum inhibition of radial growth of C. gloeosporioides (92.42%), F. nivale (73.01%) and Fusarium sp. (68.67%) were observed in case of A. niger owing to the effect of non-volatile metabolites. Trichoderma viride and A. niger may be exploited commercially as a biocontrol agent against anthracnose and fruit rot pathogens of papaya. Dhaka Univ. J. Biol. Sci. 28(1): 1-7, 2019 (January)

In-vitro Studies of Plant Extracts against Alternaria brassicae (berk.) Sacc. of Indian Mustard (Brassica juncea (L.) Czern. and coss)

2016 ◽  
Vol 3 (1) ◽  
Author(s):  
MAHESH SINGH ◽  
H. K. SINGH
Keyword(s):  
Growth Inhibition ◽  
Plant Extract ◽  
Plant Extracts ◽  
Indian Mustard ◽  
Pathogenic Fungi ◽  
Alternaria Brassicae ◽  
Test Fungus ◽  
Poison Food Technique ◽  
Percent Growth Inhibition

Six plant extract viz., Datura (Daturastramonium), Eucalyptus (Eucalyptus globulous), Karang (Pongamiaglabra),Neem (Azadirachtaindica), Madar (Calotropis gigantean) and Wild sage (Lantana camara) were evaluated in-vitro by poison food technique @ 3, 6, 9 and 12% concentrations against Alternariabrassicaecausing blight of mustard. The results revealed that all the plant extracts inhibited the percent growth inhibition against test fungus as compared to control. However A.indica was found most efficacious with growth inhibition of (38.7%) followed by E. globulus (36.7%). Some extract such as P. glabra and D. stramonium showed moderate inhibition over control (30.9 and 21.6 respectively). Among all plant extract, C. gigantean showed least (15.3%) inhibition over control against A. brassicae. These plant extracts can possibly be used as management of seedborne pathogenic fungi in an eco-friendly way.

scholarly journals In vitro evaluation of fungicides and plant extracts against pathogenic fungi of two rice varieties

2018 ◽  
Vol 44 (2) ◽  
pp. 251-259
Author(s):  
Pranami Chowdhury ◽  
MA Bashar ◽  
Shamim Shamsi
Keyword(s):  
Plant Extracts ◽  
Radial Growth ◽  
Fusarium Moniliforme ◽  
Mangifera Indica ◽  
Pathogenic Fungi ◽  
Curvularia Lunata ◽  
Asparagus Racemosus ◽  
Rice Varieties ◽  
Datura Metel

Five pathogenic fungi viz., Alternaria alternata (Fr.) Keissler, Curvularia lunata (Wakker) Boedijn, Drechslera oryzae Breda de Haan (Subramanian and Jain), Fusarium moniliforme Sheldon and Pestalotiopsis guepinii (Desm.) Stay. were isolated from two rice varieties viz., BRRI 29 (Boro) and Pajam (Aman) by Blotter and Tissue planting methods. Ten fungicides i.e. Bavistin 50 WP, Salcox 50 WP, Dithane M-45, Indofil M-45, Tall 25 EC, Ridomil MZ Gold, MC Sulphur 80, Greengel, Hayvit 80 WP and Capvit 50 WP at 100, 200, 300, 400 and 500 ppm were evaluated against the above mentioned five pathogenic fungi. Tall 25 EC completely inhibited the radial growth of the test fungi at all the concentrations except Fusarium moniliforme. Antifungal properties of ethanol extract of Artocarpus heterophyllus Lamk., Tagetes erecta L., Datura metel L., Senna alata (L.) Roxb., Azadirachta indica A. Juss., Citrus medica L., Mangifera indica L., Asparagus racemosus Willd., Nerium indicum Mill. and Allium sativum L. at 5, 10 and 20% concentrations were evaluated against the five test pathogens. All the plant extracts completely inhibited the radial growth of the test fungi at 20% concentration except Asparagus racemosus.

In Vitro Studies on the Use of Some Plant Extracts as Bio control Agents against Fusarium Oxysporum Causing Rhizome Rot of Turmeric (Curcuma longa L.) and Role of Phenolics

2018 ◽  
Vol 55 (3) ◽  
pp. 257
Author(s):  
S.K Jaffar ◽  
M. Swapna ◽  
M. Madhava Rao
Keyword(s):  
Antifungal Activity ◽  
Plant Extracts ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Curcuma Longa ◽  
Ocimum Sanctum ◽  
Total Phenolic ◽  
Rhizome Rot

Different plant extracts were studied in vitro for their efficiency of suppressing F.oxysporum causing rhizome rot of turmeric and role of Total Phenolic Content (TPC) of plant extracts in the antifungal activity, is also studied. The selected plant leaf extracts have shown significant antifungal activity (in vitro) against the mycelial growth of F.oxysporum causing rhizome rot of turmeric. Among them, Ocimum sanctum exhibited maximum antifungal activity of 99.45% inhibition over the pathogen and it has high total phenolic content of 280mg/100g. C.procera exhibited minimum inhibition percentage of 79.68. It has also least TPC of 75mg/100g.

scholarly journals Potential In Vitro Inhibition of Selected Plant Extracts against SARS-CoV-2 Chymotripsin-Like Protease (3CLPro) Activity

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1503
Author(s):  
Carla Guijarro-Real ◽  
Mariola Plazas ◽  
Adrián Rodríguez-Burruezo ◽  
Jaime Prohens ◽  
Ana Fita
Keyword(s):  
Plant Extracts ◽  
Curcuma Longa ◽  
Hydrolysis Product ◽  
Resonance Energy ◽  
Significant Inhibition ◽  
Main Role ◽  
Turmeric Extract ◽  
Ic50 Value

Antiviral treatments inhibiting Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replication may represent a strategy complementary to vaccination to fight the ongoing Coronavirus disease 19 (COVID-19) pandemic. Molecules or extracts inhibiting the SARS-CoV-2 chymotripsin-like protease (3CLPro) could contribute to reducing or suppressing SARS-CoV-2 replication. Using a targeted approach, we identified 17 plant products that are included in current and traditional cuisines as promising inhibitors of SARS-CoV-2 3CLPro activity. Methanolic extracts were evaluated in vitro for inhibition of SARS-CoV-2 3CLPro activity using a quenched fluorescence resonance energy transfer (FRET) assay. Extracts from turmeric (Curcuma longa) rhizomes, mustard (Brassica nigra) seeds, and wall rocket (Diplotaxis erucoides subsp. erucoides) at 500 µg mL−1 displayed significant inhibition of the 3CLPro activity, resulting in residual protease activities of 0.0%, 9.4%, and 14.9%, respectively. Using different extract concentrations, an IC50 value of 15.74 µg mL−1 was calculated for turmeric extract. Commercial curcumin inhibited the 3CLPro activity, but did not fully account for the inhibitory effect of turmeric rhizomes extracts, suggesting that other components of the turmeric extract must also play a main role in inhibiting the 3CLPro activity. Sinigrin, a major glucosinolate present in mustard seeds and wall rocket, did not have relevant 3CLPro inhibitory activity; however, its hydrolysis product allyl isothiocyanate had an IC50 value of 41.43 µg mL−1. The current study identifies plant extracts and molecules that can be of interest in the search for treatments against COVID-19, acting as a basis for future chemical, in vivo, and clinical trials.

scholarly journals Botanicals to Control Soft Rot Bacteria of Potato

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
M. M. Rahman ◽  
A. A. Khan ◽  
M. E. Ali ◽  
I. H. Mian ◽  
A. M. Akanda ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Curcuma Longa ◽  
Soft Rot ◽  
Storage Condition ◽  
Storage Conditions ◽  
Leaf Extracts ◽  
Corchorus Capsularis ◽  
Rot Disease ◽  
And Storage

Extracts from eleven different plant species such as jute (Corchorus capsularisL.), cheerota (Swertia chiraitaHam.), chatim (Alstonia scholarisL.), mander (Erythrina variegata), bael (Aegle marmelosL.), marigold (Tagetes erecta), onion (Allium cepa), garlic (Allium sativumL.), neem (Azadiracta indica), lime (Citrus aurantifolia), and turmeric (Curcuma longaL.) were tested for antibacterial activity against potato soft rot bacteria,E. carotovorasubsp.carotovora (Ecc)P-138, underin vitroand storage conditions. Previously,EccP-138 was identified as the most aggressive soft rot bacterium in Bangladeshi potatoes. Of the 11 different plant extracts, only extracts from dried jute leaves and cheerota significantly inhibited growth ofEccP-138in vitro. Finally, both plant extracts were tested to control the soft rot disease of potato tuber under storage conditions. In a 22-week storage condition, the treated potatoes were significantly more protected against the soft rot infection than those of untreated samples in terms of infection rate and weight loss. The jute leaf extracts showed more pronounced inhibitory effects onEcc-138 growth both inin vitroand storage experiments.

scholarly journals In vitro evaluation of fungicides and some plant extracts against rice sheath rot pathogen Sarocladium oryzae

2016 ◽  
Vol 29 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Shamim Shamsi ◽  
Pranami Chowdhury
Keyword(s):  
Plant Extracts ◽  
Radial Growth ◽  
Mangifera Indica ◽  
Ethanol Extract ◽  
Tagetes Erecta ◽  
Asparagus Racemosus ◽  
Test Fungus ◽  
Datura Metel ◽  
Sheath Rot

Sarocladium oryzae (Sawada) W. Gams & D. Hawksworth, the causal agent of sheath rot of rice was isolated from sheath rot infected rice sheaths and grains. Among ten tested fungicides Tall 25 EC completely inhibited radial growth of the fungus even at the lowest concentration 100 ppm. Similarly ethanol leaf extract of ten plants, namely Allium sativum, Artocarpus heterophyllus, Asparagus racemosus, Azadirachta indica, Citrus medica, Datura metel, Mangifera indica, Nerium indicum, Senna alata and Tagetes erecta at 5, 10 and 20% concentrations were screened for their fungicidal activity against the test fungus. All the plant extracts completely inhibited the radial growth of the test fungus at 20% concentration except Asparagus racemosus and C. medica. Ethanol extract of Tagetes erecta and Mangifera indica also completely inhibited the radial growth of the test fungus at 10% concentration.Bangladesh J. Sci. Res. 29(1): 47-54, June-2016

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