scholarly journals Structural Analysis of a Polysaccharide Isolated from the Alkali Extract of an Edible Mushroom, Pleurotus sajor-caju, Cultivar Black Japan

2021 ◽  
Vol 13 (3) ◽  
pp. 1025-1041
Author(s):  
A. K. Ojha
Keyword(s):  
Edible Mushroom ◽  
Molar Ratio ◽  
Methylation Analysis ◽  
Total Acid ◽  
Ps Ii ◽  
Alkaline Extract ◽  
Ps I ◽  
Alkali Extract ◽  
Insoluble Glucan ◽  
Pleurotus Sajor Caju

A heteropolysaccharide (PS-I) and an insoluble glucan (PS-II) were isolated from the alkaline extract of an edible mushroom   Pleurotus sajor-caju, cultivar Black Japan. The polysaccharide (PS-I) was found to consist of D-glucose and D-galactose in a molar ratio of 3:1. Based on total acid hydrolysis, methylation analysis, and NMR experiments (1H, 13C, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit of the polysaccharide was established.  

Isolierung von PS II-Partikeln mit in vivo Eigenschaften aus Euglena gracilis, Stamm Z / Isolation of PS II-Particels with in vivo Characteristics from Euglena gracilis, Stamm Z

1982 ◽  
Vol 37 (3-4) ◽  
pp. 256-259 ◽  
Author(s):  
F. Schuler ◽  
P. Brandt ◽  
W. Wießner
Keyword(s):  
Euglena Gracilis ◽  
Fluorescence Emission ◽  
Improved Method ◽  
Ps Ii ◽  
Ps I ◽  
Emission And Absorption Spectra ◽  
Chlorophyll Protein ◽  
Ps Ii Activity ◽  
Photosystem Ii Particles

Abstract An improved method for isolation of (photosystem II)-particles from Euglena gracilis, strain Z was established. PS II-particles isolated by ultrasonic treatment and following differential centrifugation show fluorescence emission and absorption spectra identical with in vivo properties of Euglena gracilis. These PS II-particles have only PS II-activity and contain CP a, the typical chlorophyll-protein-complex of PS II. No contamination of PS I-components are detectable.

Photoinactivation of Photosynthetic Electron Transport under Anaerobic and Aerobic Conditions in Isolated Thylakoids of Spinach

1992 ◽  
Vol 47 (1-2) ◽  
pp. 63-68 ◽  
Author(s):  
Rekha Chaturvedi ◽  
M. Singh ◽  
P. V. Sane
Keyword(s):  
Electron Transport ◽  
Photosynthetic Electron Transport ◽  
Oxygen Evolving Complex ◽  
Reaction Centre ◽  
Ps Ii ◽  
Ps I ◽  
S2 State ◽  
The Stability ◽  
Oxygen Evolving ◽  
Spinach Thylakoids

Abstract The effect of exposure to strong white light on photosynthetic electron transport reactions of PS I and PS II were investigated in spinach thylakoids in the absence or presence of oxygen. Irrespective of the conditions used for photoinactivation, the damage to PS II was always much more than to PS I. Photoinactivation was severe under anaerobic conditions compared to that in air for the same duration. This shows that the presence of oxygen is required for prevention of photoinactivation of thylakoids. The susceptibility of water-splitting complex in photoinactivation is indicated by our data from experiments with chloride-deficient chloroplast membranes wherein it was observed that the whole chain electron transport from DPC to MV was much less photoinhibited than that from water. The data from the photoinactivation experiments with the Tris-treated thylakoids indicate another photodam age site at or near reaction centre of PS II. DCMU-protected PS II and oxygen-evolving complex from photoinactivation. DCMU protection can also be interpreted in terms of the stability of the PS II complex when it is in S2 state.

scholarly journals The chlorophyll a/b -proteins of PS I and PS II are immunologically related

FEBS Letters ◽  
1986 ◽  
Vol 199 (2) ◽  
pp. 227-233 ◽  
Author(s):  
Paula K. Evans ◽  
Jan M. Anderson
Keyword(s):  
Chlorophyll A ◽  
Ps Ii ◽  
Ps I

scholarly journals Determination of the PS I content of PS II core preparations using selective emission: A new emission of PS II at 780nm

2014 ◽  
Vol 1837 (1) ◽  
pp. 167-177 ◽  
Author(s):  
Jennifer Morton ◽  
Jeremy Hall ◽  
Paul Smith ◽  
Fusamichi Akita ◽  
Faisal Hammad Mekky Koua ◽  
...  
Keyword(s):  
Ps Ii ◽  
Ps I ◽  
Ps Ii Core

scholarly journals Proton flow through the ATP synthase in chloroplasis regulates the distribution of light energy between PS I and PS II

FEBS Letters ◽  
1991 ◽  
Vol 282 (2) ◽  
pp. 449-449
Author(s):  
G. Braun ◽  
Y. Evron ◽  
S. Malkin ◽  
M. Avron
Keyword(s):  
Atp Synthase ◽  
Light Energy ◽  
Ps Ii ◽  
Ps I ◽  
Flow Through

Isolation and characterization of an immunoenhancing glucan from alkaline extract of an edible mushroom, Lentinus squarrosulus (Mont.) Singer

2011 ◽  
Vol 346 (13) ◽  
pp. 2039-2044 ◽  
Author(s):  
Sanjoy Kumar Bhunia ◽  
Biswajit Dey ◽  
Kankan K. Maity ◽  
Sukesh Patra ◽  
Soumitra Mandal ◽  
...  
Keyword(s):  
Edible Mushroom ◽  
Alkaline Extract ◽  
Isolation And Characterization

Is the in vivo Photosystem I Function Resistant to Photoinhibition? An Answer from Photoacoustic and Far-Red Absorbance Measurements in Intact Leaves

1991 ◽  
Vol 46 (11-12) ◽  
pp. 1038-1044 ◽  
Author(s):  
Michel Havaux ◽  
Murielle Eyletters
Keyword(s):  
Light Stress ◽  
Red Light ◽  
Light Treatment ◽  
High Light ◽  
Saturation Curve ◽  
Strong Light ◽  
Ps Ii ◽  
Fluorescence Measurements ◽  
Ps I

Abstract Preillumination of intact pea leaves with a strong blue-green light of 400 W m-2 markedly inhibited both photoacoustically monitored O2-evolution activity and PS II photochemistry as estimated from chlorophyll fluorescence measurements. The aim of the present work was to examine, with the help of the photoacoustic technique, whether this high-light treatment deteriorated the in vivo PS I function too. High-frequency photoacoustic measurements indicated that photochemical conversion of far-red light energy in PS I was preserved (and even transiently stimulated) whereas photochemical energy storage monitored in light exciting both PS I and PS II was markedly diminished. Low-frequency photoacoustic measurements of the Emerson enhancement showed a spectacular change in the PS II/PS I activity balance in favor of PS I. It was also observed that the linear portion of the saturation curve of the far-red light effect in the Emerson enhancement was not changed by the light treatment. Those results lead to the conclusion that, in contrast to PS II, the in vivo PS I photofunctioning was resistant to strong light stress, thus confirming previous suggestions derived from in vitro studies. Estimation of the redox state of the PS I reaction center by leaf absorbance measurements at ca. 820 nm suggested that, under steady illumination, a considerably larger fraction of PS I centers were in the closed state in high-light pretreated leaves as compared to control leaves, presumably allowing passive adjustment of the macroscopic quantum yield of PS I photochemis­ try to the strongly reduced photochemical efficiency of photoinhibited PS II.

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