scholarly journals Cytotoxicity Assessment of Heritiera Littoralis (Aiton), Madhuca Longifolia (König) Macbr., Nerium Indicum Mill. And Sapium Indicum (Willd.) Leaves on Artemia Salina (L.)

2021 ◽  
Vol 29 (2) ◽  
pp. 139-149
Author(s):  
N Fatema ◽  
K Hasan ◽  
A Islam ◽  
N Yasmin ◽  
M Nesa ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Artemia Salina ◽  
Ether Extract ◽  
Chcl3 Extract ◽  
Madhuca Longifolia ◽  
Cytotoxicity Assessment

Petroleum ether, CHCl3 and CH3OH extracts of leaves of Heritiera littoralis (Aiton), Madhuca longifolia (König) Macbr., Nerium indicum Mill. and Sapium indicum (Willd.) were subjected to assess cytotoxicity against Artemia salina (L.) nauplii. The petroleum ether extract of H. littoralis leaves showed LC50 values 273.77, 97.27, 51.60, 37.12, 14.60 and 12.59 ppm after 12, 18, 24, 30, 36 and 42 h; the CHCl3 extract showed LC50 values 733.25, 105.51, 40.72 and 18.20 ppm after 6, 12, 18 and 24 h whereas CH3OH extract showed 73.05, 30.62, 24.56, 20.85, 16.21 and 6.71 ppm after 6, 12, 18, 24, 30 and 36 h of exposure respectively. The petroleum ether extract of M. longifolia leaves possess LC50 values 259.35, 115.17, 56.84 and 8.73 ppm after 12, 18, 24 and 30 h; the CHCl3 extract possess LC50 values 585.43, 205.86, 112.74, 75.62, 52.84and 47.34 ppm after 12, 18, 24, 30, 36 and 42 h but CH3OH extract possess LC50 values 185.87, 60.70, 30.11 and 15.39 ppm after 12, 18, 24 and 30 h of exposure respectively. The petroleum ether extract of N. Indicum leaves recorded LC50 values 249.82, 146.07, 80.23, 54.21 and 40.19 ppm after 18, 24, 30, 36 and 42 h; the CHCl3 extract gave LC50 values 36.13, 21.72, 19.03, 16.81 and 16.34 ppm after 12, 18, 24, 30 and 36 h but CH3OH extract recorded LC50 values 394.90, 129.69, 81.50, 73.10 and 37.51 ppm after 18, 24, 30, 36 and 42 h of exposure respectively. Similarly, the petroleum ether extract of S. indicum leaves showed LC50 values 24.79, 13.18 and 4.61 ppm after 12, 18 and 24 h; the CHCl3 extract were 50.45, 42.64, 21.20 and 14.93 ppm after 18, 24, 30 and 36 h of exposure and the CH3OH extract showed LC50 values 306.37, 217.18, 149.38, 73.52, 54.45 and 22.91 ppm after 12, 18, 24, 30, 36 and 42 h of exposure respectively. The intensity of efficacy of the extracts could be arranged in the following descending order of S. indicum (petroleum etroleum ether extract) >H. littoralis (CH3OH extract) >M. longifolia (petroleum ether extract) >N. indicum (CHCl3 extract). J. Bio-Sci. 29(2): 139-149, 2021 (December)

scholarly journals Bioactive Potentials of Cleome viscosa L. Extracts: Dose-mortality, Insect Repellency and Brine Shrimp Lethality

2017 ◽  
Vol 9 (4) ◽  
pp. 375-382
Author(s):  
S. A. Rimi ◽  
S. Hossain ◽  
S. Islam ◽  
Z. Islam ◽  
S. B. Chhabi ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Tribolium Castaneum ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Callosobruchus Chinensis ◽  
Cytotoxicity Test ◽  
Ether Extract ◽  
Chcl3 Extract ◽  
Level Of Significance ◽  
Cleome Viscosa

Petroleum ether (Pet. ether), chloroform (CHCl3) and methanol (CH3OH) extracts of Cleome viscosa have thoroughly screened against Tribolium castaneum (Hbst.) for repellency and dose mortality, against Callosobruchus chinensis for dose mortality activities, and against Artemia salina nauplii for lethality. In case of repellency of T. castaneum, Pet. ether extract found significantly active at 1% level of significance (P < 0.01), CHCl3 extract offered activity at 5% level of significance (P < 0.05) and CH3OH extract did not show any activity at all. In case of mortality against T. castaneum  the LD50 values of Pet. ether, CHCl3, and CH3OH extracts were 2.380, 2.188, 1.867, 1.658 and 1.429 mg/cm2; 4.950, 2.906, 2.664, 2.658 and 2.609 mg/cm2; and 4.950 and 2.791, 2.609, 2.552 and 2.474 mg/cm2 after 0.5, 6, 12, 18 and 24 h of exposure respectively.  Against C. chinensis the LD50 values of the same extracts were 0.781, 0.670, 0.642, 0.614, 0.560 and 0.494 mg/cm2; 1.964, 1.050, 0.867, 0.725, 0.635 and 0.590 mg/cm2; and 2.631, 1.851, 1.471, 1.703, 1.524 and 1.457 mg/cm2 after  6, 12, 18, 24, 30 and 36 h of exposure respectively. In case of cytotoxicity test against A. salina, the extracts offered LC50 values in a descending order CH3OH > CHCl3 > Pet. ether extracts.

scholarly journals Antimicrobial and Cytotoxic Activities of Root Extracts of Piper Chaba

2008 ◽  
Vol 1 (1) ◽  
pp. 138-144 ◽  
Author(s):  
Tarannum Naz ◽  
Ashik Mosaddik ◽  
M. Ekramul Haque
Keyword(s):  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Primary Standard ◽  
Artemia Salina ◽  
Diffusion Method ◽  
Cytotoxic Activities ◽  
Ether Extract ◽  
Antifungal Properties ◽  
Bacteria And Fungi ◽  
Antibacterial And Antifungal

  Antibacterial and antifungal properties of petroleum ether, chloroform, ethyl acetate and methanol extracts of Piper chaba (Choi) roots were studied by disc diffusion method and these activities were compared with primary standard drugs Kanamycin and Nystatin, respectively. The extracts were found to exhibit promising antibacterial and antifungal properties against Gram-positive, Gram-negative bacteria and fungi. The extracts were also studied for their cytotoxic activities by brine shrimp lethality bioassay, where gallic acid was used as primary standard. It was observed that the petroleum ether extract was potent cytotoxic with the LC50 value of 0.95 µg/ml against Artemia salina (L). The essential oils of the petroleum ether extract of Piper chaba roots were analysed by GC/MS. It was observed that most of the compounds were sesquiterpenes, some were long chain fatty acids and some were monoterpenes and alkaloids. Seventeen compounds were identified from the GC/MS analysis. Keywords: Piper chaba, antibacterial activity, antifungal activity, cytotoxicity. © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. DOI: 10.3329/jsr.v1i1.1446

Antioxidant lignans from petroleum ether extract of Schisandra chinensis

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
J Slanina ◽  
L Bøezinová ◽  
H Paulová ◽  
O Humpa
Keyword(s):  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Schisandra Chinensis ◽  
Ether Extract

The struggle with rheumatism through Dracunculus vulgaris Schott: In the light of ethnobotanical information.

2020 ◽  
Vol 13 ◽  
Author(s):  
Fatma Tuğçe Gürağaç Dereli ◽  
Mert Ilhan ◽  
Esra Küpeli Akkol
Keyword(s):  
Petroleum Ether ◽  
Inflammatory Diseases ◽  
Petroleum Ether Extract ◽  
Major Constituent ◽  
Control Group ◽  
Ether Extract ◽  
Test Models ◽  
Mouse Ear ◽  
Gas Chromatography Mass Spectroscopy ◽  
Abdominal Constriction

Background: The fruits with the seeds of Dracunculus vulgaris Schott. (Araceae) are used against inflammatory diseases in Turkey. Objective: Present study was designed to justify this folkloric usage type of the plant. Therefore, the aim of this study is to investigate the anti-inflammatory activity of D. vulgaris. Methods: Petroleum ether, ethyl acetate and methanol extracts were prepared from the fruits, successively. Carrageenan-, serotonin-, and prostaglandin E2-induced hind paw edema; acetic acid–induced capillary permeability and 12-O-tetradecanoyl-phorbol-13-acetate–induced mouse ear edema models were used to assess the antiinflammatory activity of the extracts. The analgesic activity was experienced by using p-benzoquinone-induced abdominal constriction test. Results: The petroleum ether extract displayed the highest activities in all of the used test models compared with the control group. Therefore, the constituents of this extract were determined by using gas chromatography–mass spectroscopy (GC–MS). Linoleic acid was found to be major constituent of the petroleum ether extract of D. vulgaris. Conclusion: This study has provided some justification for the folkloric use of the plant.

scholarly journals The petroleum ether extraction of Brassica Rapa L. induces apoptosis of Lung adenocarcinoma cells via mitochondria-dependent pathway

2021 ◽  
Author(s):  
Xierenguli Halike ◽  
Jin-Yu Li ◽  
Pengfei Yuan ◽  
Kaimeiliya Yasheng ◽  
Min Chen ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Brassica Rapa ◽  
Biological Activities ◽  
Petroleum Ether Extract ◽  
Traditional Food ◽  
Ether Extract ◽  
Ether Extraction ◽  
Adenocarcinoma Cells ◽  
Lung Adenocarcinoma Cells ◽  
Dependent Pathway

Brassica rapa L. is one of the most popular traditional food with a variety of biological activities. In this study, the petroleum ether extract of B. rapa was separated by...

Identification of phytoconstituents of Tragia involucrata leaf extracts and evaluate their correlation with anti-inflammatory & antioxidant properties

2021 ◽  
Vol 20 ◽  
Author(s):  
Vinodhini Velu ◽  
Swagata Banerjee ◽  
Vidya Rajendran ◽  
Gaurav Gupta ◽  
Dinesh Kumar Chellappan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethyl Acetate Extract ◽  
Petroleum Ether Extract ◽  
Inflammatory Activity ◽  
Ether Extract ◽  
Membrane Stabilization ◽  
Leaf Extracts ◽  
Anti Inflammatory

Aims: The present investigation was aimed at exploring the phytoconstituents using Gas Chromatography Mass Spectroscopy and to evaluate antioxidant and anti-inflammatory properties of the leaf extracts. Materials and Methods: The extracts were obtained sequentially with petroleum ether, ethyl acetate and water using soxhlet apparatus. The anti-inflammatory property of the identified compounds using GC- MS spectroscopy was evaluated in silico. The antioxidant activity was performed by DPPH and H2O2 method whereas anti-inflammatory study was carried out by HRBC membrane stabilization method. Terpenoids were found to be major constituents in petroleum ether extract while, phenols and flavonoids were predominantly found in ethyl acetate extract. Results and Discussion: The GC-MS analysis of the extract revealed six major molecules including Squalene, 19β, 28- epoxyleanan-3-ol and 2-tu-Butyl-5-chloromethyl-3-methyl-4-oxoimidazolidine-1-carboxylic acid. The ethyl acetate extract showed a significant antioxidant activity (P<0.01) in both DPPH method (70.87 %) and H2O2 method (73.58%) at 200 µg mL-1 . Increased membrane stabilization of petroleum ether extract was observed in the in vitro anti-inflammatory activity study. A strong relationship between the terpenoid content and anti-inflammatory activity was obtained from the correlation (0.971) and docking study. Conclusion: These results justify T. involucrata to be a rich source of terpenoids with potent antiinflammatory property.

scholarly journals Evaluation of hair growth promoting activity of petroleum ether extract of Abrus precatorius Linn. on Wistar Albino rats

2019 ◽  
Vol 9 (2) ◽  
pp. 330-335
Author(s):  
Namrata Omhare ◽  
Pratibha Sahu ◽  
Shailee Gautam ◽  
Nidhi Jain
Keyword(s):  
Petroleum Ether ◽  
Hair Growth ◽  
Petroleum Ether Extract ◽  
Hair Follicles ◽  
Ether Extract ◽  
Standard Drug ◽  
Abrus Precatorius ◽  
Growth Initiation ◽  
Growth Promoting ◽  
Bald Patch

In present investigation was carried out to screening of hair growth promoting potentiality of petroleum ether extract of Abrus precatorius leaf. Preliminary chemical tests and TLC analysis revealed the presence flavonoids and saponins. Hair growth promoting activity of petroleum ether of Abrus precatorius was screened by considering different parameters which included time taken for covering bald patch, length of hair produced, percentage of hair follicles in anagen and telogen phases, time of hair growth initiation and completion and level of minerals in blood. The petroleum ether extract of Abrus precatorius showed a very good hair growth promoting activity at a dose of 300 mg/kg which was comparable to that of 2% minoxidil. After 30 days of treatment with test and standard drugs it was observed that, time taken for covering the bald patch, hair growth initiation and completion time and quantitative hair growth were found to be comparable to that of the standard drug. An increase in percentage of hair follicles turning from telogen phase to anagen phase was noted. The control treated group of animals showed poor hair growth for all the parameters. Keywords: Abrus precatorius Linn, Anagen, Catagen, Telogen, Minoxidil

scholarly journals Termite-Repellent Activity and Chemical Composition of West Indian Mahogany Wood, (Swietenia Mahagoni Jacq.) with Special Reference to the P2 Fraction

1969 ◽  
Vol 42 (3) ◽  
pp. 185-195
Author(s):  
Conrado F. Asenjo ◽  
Luis Amorós Marín ◽  
Wanda Torres ◽  
Alice Del Campillo
Keyword(s):  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
West Indian ◽  
Repellent Activity ◽  
Structural Level ◽  
Ether Extract ◽  
Selective Solvents ◽  
Ether Extraction ◽  
Termite Attack ◽  
Waxy Substance

1. Serial extraction of West Indian mahogany wood with selective solvents was performed. 2. The petroleum ether extract was the only fraction to exhibit promising termite-repellent activity. 3. Mahogany blocks subjected to petroleum ether extraction became susceptible to termite attack. 4. A waxy substance (P2 fraction) separated from the petroleum ether extract at times showed high termite-repellent activity. The termite-repellent principle in the P2 fraction seems to be a labile one, as the termite-repellent activity varied greatly from batch to batch. 5. The P2 fraction is a mixture of two major components and of at least a third minor one. As far as we have been able to determine this mixture, at the elemental level, contains carbon, hydrogen, and oxygen only, while esters have been the only compounds definitely characterized at the structural level.

scholarly journals Phytochemical studies of the herb, Tragopogon orientalis L. (Asteraceae). 1. The components of a petroleum ether extract

2014 ◽  
Vol 57 (1) ◽  
pp. 85-92 ◽  
Author(s):  
Tadeusz Krzaczek ◽  
Helena D. Smolarz
Keyword(s):  
Gas Chromatography ◽  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Crystalline Form ◽  
Aliphatic Alcohols ◽  
Ether Extract ◽  
Phytochemical Studies

Hydrocarbons, aliphatic alcohols, triterpenes and sterols were shown to occur in the herb, <em>Tragopogon orientalis</em> L. The following were obtained in crystalline form: n-triacontan, hexacosanol, (β-amyrin and lupeol. The presence of (β-sitosterol, stigmasterol, cholesterol and four unidentified sterols was demonstrated by gas chromatography.

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