scholarly journals Fascioliasis-An Uncommon Cause of Recurrent Cholangitis

2019 ◽  
Vol 19 (1) ◽  
pp. 35-38
Author(s):  
Bidhan C Das ◽  
Arif Salam Khan ◽  
Md Saief Uddin ◽  
Md Mohsen Chowdhury ◽  
Md Zulfiqur Rahman Khan
Keyword(s):  
East Asia ◽  
Pancreatic Surgery ◽  
Fasciola Hepatica ◽  
Far East ◽  
Biliary Tree ◽  
Definite Diagnosis ◽  
Liver Flukes ◽  
Medical University

We report a case of Fascioliasis presented with recurrent cholangitis in a female necessitating multiple consultations and hospitalizations over a period of two years. Investigative profile failed to confirm any definite diagnosis prior to her admission in Hepato-Biliary-Pancreatic Surgery Division of the Department of Surgery of Bangabandhu Sheikh Mujib Medical University where she was provisionally diagnosed as a case suffering from extrahepatic cholagiocarcinoma. With the intent of extrahepatic excision with Rouxen-Y hepaticojejunostomy abdomen was explored and surprisingly 28 mature liver flukes (fasciola hepatica) were found lodged in the extra and intrahepatic biliary tree. Although fasciola hepatica infestation is more common in far East Asia, this case may represent the tip of an iceberg of endemic infestation of this trematode in Bangladesh which warrants further investigations. Journal of Surgical Sciences (2015) Vol. 19 (1) : 35-38

Molecular Characterization of Fasciola spp. from Some Parts of Iran

2020 ◽  
Author(s):  
Hamid HASANPOUR ◽  
Reza FALAK ◽  
Saied Reza NADDAF ◽  
Santiago MAS-COMA ◽  
Mohammad Bagher ROKNI ◽  
...  
Keyword(s):  
East Asia ◽  
Fasciola Hepatica ◽  
Genetic Difference ◽  
Rflp Analysis ◽  
Restriction Fragment Polymorphism ◽  
Pcr Rflp ◽  
The World ◽  
Liver Flukes ◽  
Fasciola Spp ◽  
Different Parts

Background: Identification of liver flukes, Fasciola hepatica, and Fasciola gigantica by morphometric parameters is not always reliable due to the overlapping measurements. This study aimed to characterize the liver flukes of animals from different parts of Iran by the genetic markers, ITS1, and COXI. Methods: We collected flukes from infected livestock in six provinces of Iran from Sep to Nov 2016. The flukes were identified by amplification of a 680 bp sequence of ITS1 locus followed by a restriction fragment polymorphism (RFLP) assay. The genetic diversity among isolates was evaluated by amplification and sequencing of a 493 bp fragment of the COXI gene. Results: We obtained 38 specimens from Khuzestan, 22 from Tehran, 10 from Isfahan, 10 from Mazandaran, 4 from Kurdistan, and 3 from Ardabil provinces. PCR-RFLP analysis revealed two patterns, representing F. hepatica, and F. gigantica. Fifty specimens from cattle and sheep exhibited F. hepatica pattern and 37 from the cattle, sheep, buffalo, and goat that of F. gigantica. The phylogeny based on COXI revealed two distinct clades separating F. hepatica from F. gigantica. In our phylogeny, the Iranian F. gigantica isolates showed a distinct separation from the African flukes, while grouped with the East Asia specimens demonstrating a common ancestor. The F. hepatica isolates clustered with the flukes from different parts of the world, including East Asia, Europe, and South America. Conclusion: The present study revealed a substantial genetic difference between F. gigantica populations of Asia and Africa, while F. hepatica isolates from different parts of the world shared high similarities.

The first lichenological survey in Koryakia (Northern Kamchatka, Russia) — the last unexplored part of Beringia

2019 ◽  
Vol 53 (1) ◽  
pp. 107-142 ◽  
Author(s):  
D. E. Himelbrant ◽  
I. S. Stepanchikova ◽  
T. Ahti ◽  
V. Yu. Neshataeva
Keyword(s):  
East Asia ◽  
Russian Far East ◽  
Far East ◽  
Kamchatka Peninsula ◽  
Lichen Diversity ◽  
Magadan Region ◽  
Specific Species ◽  
First Time ◽  
Lichen Flora

The first lichenological inventory in Koryakia has resulted in the list of 315 species reported from Parapolsky Dale, within and in vicinities of the Koryak State Reserve. Altogether 46 species are published from the Kamchatka Territory for the first time, including Lecanographa grumulosa new to Russia, East Asia and Beringia; Cercidospora trypetheliza, Lecania dubitans, Pertusaria borealis, Piccolia ochrophora, Protoparmelia cupreobadia, Rimularia badioatra and Strangospora moriformis new to Russian Far East; Abrothallus bertianus, Cladonia strepsilis, Physciella melanchra, Rimularia badioatra, Sclerococcum parasiticum, Sphinctrina leucopoda and Strangospora moriformis new to Beringia. The lichen diversity of the study area is relatively poor due to natural reasons. Comparison with neighboring regions (Kamchatka Peninsula, Chukotka, Magadan Region, Yakutia and Alaska) shows that the lichen flora of Parapolsky Dale contains almost no specific species. The majority of the species recorded here are also known from neighboring regions, especially Alaska and Kamchatka Peninsula.

Tersilochinae of South, Southeast and East Asia, excluding Mongolia and Japan (Hymenoptera: Ichneumonidae)

2011 ◽  
Vol 20 (1) ◽  
pp. 96-148 ◽  
Author(s):  
A.I. Khalaim
Keyword(s):  
Sri Lanka ◽  
South Korea ◽  
East Asia ◽  
Far East ◽  
New Combination ◽  
East China ◽  
Far East Of Russia ◽  
Undescribed Species ◽  
Oriental Region ◽  
Unidentified Species

Tersilochines of South, Southeast and East Asia (excluding Mongolia and Japan) have been studied. Eight genera and 60 species are recorded in the region: Allophrys (2 species), Barycnemis (5 species), Diaparsis (29 species), Phradis (2 species, including 1 unidentified species), Probles (12 species, including 1 unidentified and 6 undescribed species), Sathropterus (2 species), Slonopotamus gen. nov. (2 species) and Tersilochus (6 species, including one species of the obscure status). One genus and 26 species are described as new: Allophrys bruneiensis sp. nov. (Brunei), A. occipitata sp. nov. (Vietnam, India), Diaparsis absista sp. nov. (Brunei), D. bannapeana sp. nov. (Laos), D. bolikhamsaica sp. nov. (Laos, Thailand), D. brunnea sp. nov. (Brunei), D. crenulator sp. nov. (Brunei), D. dediticia sp. nov. (Vietnam, Brunei), D. hilaris sp. nov. (Vietnam), D. karnatakana sp. nov. (India), D. labiensis sp. nov. (Brunei), D. mandibulator sp. nov. (Laos), D. minuta sp. nov. (Vietnam), D. monstrosa sp. nov. (Brunei), D. morleyi sp. nov. (Sri Lanka), D. propodeator sp. nov. (Brunei, Sarawak State of Malaysia, southern Indonesia, Laos), D. pulchra sp. nov. (South Korea), D. sarawakiensis sp. nov. (Sarawak and Pahang states of Malaysia), D. viela sp. nov. (Vietnam, Laos), D. vietnamica sp. nov. (Vietnam), D. zispina sp. nov. (Vietnam), Probles vietnamica sp. nov. (Vietnam, probably East China and south of Far East of Russia), Sathropterus secundus sp. nov. (Vietnam), Slonopotamus elephantoides sp. nov. (Laos), S. indianus sp. nov. (India) and Tersilochus granulatus sp. nov. (South Korea). Generic assignment of two species are changed: Barycnemis sanctijohanni (Rao & Kurian, 1951), new combination, and Probles (Microdiaparsis) caudate (Morley, 1913), new combination. Barycnemis dissimilis and B. tobiasi from Nepal, Diaparsis convexa from Vietnam, D. niphadoctona from Laos, and Sathropterus pumilus from India and Nepal are newly recorded from the countries. The genus Diaparsis comprises almost half of species of the tersilochine fauna of the studied region (29 species, 48%), and is a dominant genus in the Oriental Region. Keys to genera and species of Tersilochinae of South, Southeast and East Asia (excluding Mongolia and Japan) are provided.

scholarly journals Selection of vaccine strains for serotype O foot-and-mouth disease viruses (2007–2012) circulating in Southeast Asia, East Asia and Far East

Vaccine ◽  
2017 ◽  
Vol 35 (51) ◽  
pp. 7147-7153 ◽  
Author(s):  
Mana Mahapatra ◽  
Sasmita Upadhyaya ◽  
Sharie Aviso ◽  
Aravindh Babu ◽  
Geoff Hutchings ◽  
...  
Keyword(s):  
Southeast Asia ◽  
East Asia ◽  
Far East ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Serotype O ◽  
Foot And Mouth ◽  
Selection Of

A Life of Sir Harry Parkes

2020 ◽  
Author(s):  
Robert Morton
Keyword(s):  
East Asia ◽  
Civil Service ◽  
Far East ◽  
Working Life ◽  
Professional Life ◽  
The Far East ◽  
History Of

Harry Parkes was at the heart of Britain’s relations with the Far East from the start of his working life at fourteen, to his death at fifty-seven. Orphaned at the age of five, he went to China on his own as a child and worked his way to the top. God-fearing and fearless, he believed his mission was to bring trade and ‘civilisation’ to East Asia. In his day, he was seen as both a hero and a monster and is still bitterly resented in China for his part in the country’s humiliations at Western hands, but largely esteemed in Japan for helping it to industrialise. Morton’s new biography, the first in over thirty years, and benefiting in part from access to the Parkes’ family and archives, offers a more intimate and informed profile of the personal and professional life of a Victorian titan and one of Britain’s most undiplomatic diplomats in the history of the British Civil Service.

scholarly journals The origins of pottery in East Asia: updated analysis (the 2015 state-of-the-art)

2015 ◽  
Vol 42 ◽  
Author(s):  
Yaroslav Kuzmin
Keyword(s):  
East Asia ◽  
South China ◽  
State Of The Art ◽  
Russian Far East ◽  
Critical Evaluation ◽  
Far East ◽  
Transbaikal Region ◽  
Recent Developments ◽  
Japanese Islands ◽  
The Russian Far East

Recent developments related to the emergence of pottery in East Asia and neighbouring regions are presented. According to a critical evaluation of the existing evidence, the oldest centres with pottery in East Asia are situated in South China (dated to c. 18 000 calBP), the Japanese Islands (c. 16 700 calBP), and the Russian Far East (c. 15 900 calBP). It is most likely that pottery-making appeared in these regions independently of each other. In Siberia, the earliest pottery now known isfrom the Transbaikal region (dated to c. 14 000 calBP). However, it did not influence the more westerly parts of Siberia in terms of the origin and spread of pottery-making.

scholarly journals Specific Detection of Fasciola hepatica and F. gigantica in Infected Domesticated Animals Using High-Resolution Melting Analysis (HRM)

2020 ◽  
Author(s):  
Zeinab MOGHADAMIZAD ◽  
Ahmad HOSSEINI-SAFA ◽  
Mehdi MOHEBALI ◽  
Peyman HEYDARIAN ◽  
Mojgan ARYAEIPOUR ◽  
...  
Keyword(s):  
High Resolution ◽  
High Resolution Melting ◽  
Fasciola Hepatica ◽  
High Resolution Melting Analysis ◽  
Universal Primers ◽  
Specific Primer ◽  
Universal Primer ◽  
Melting Analysis ◽  
Liver Flukes ◽  
Fasciola Spp

Background: It is difficult to make an exact morphological distinction between Fasciola hepatica and Fasciola gigantica. We used High Resolution Melting analysis (HRM) method to differentiate the F. hepatica species from F. gigantica in order to differentiate them. Methods: Overall, 80 adult liver flukes were collected from infected slaughtered animals including cattle, sheep and goats from Lorestan Province, western Iran from Sep 2015 to Aug 2017. Genomic DNA was extracted using commercial DNA extraction kit. The multilocus sequences of mDNA including COX1, COX3 and ND6 were amplified employing real-time PCR & HRM analysis. Specific and universal primer pairs were designed for differentiation Fasciola spp. Results: Universal primers cannot be used to distinguish between these two species, but in the contrary, specific primer pairs of each species could differentiate them properly. Molecular identification using specific primer pairs were consistent. Conclusion: HRM is a simple, fast and reliable method for detecting and differentiating F. hepatica from F. gigantica and can be used for diagnostic and epidemiological purposes.

Characterisation of two calmodulin-like proteins from the liver fluke, Fasciola hepatica

2007 ◽  
Vol 388 (6) ◽  
pp. 593-599 ◽  
Author(s):  
Sean L. Russell ◽  
Neil V. McFerran ◽  
Elizabeth M. Hoey ◽  
Alan Trudgett ◽  
David J. Timson
Keyword(s):  
Metal Binding ◽  
Metal Ion ◽  
Liver Fluke ◽  
Fasciola Hepatica ◽  
Expression Vectors ◽  
Metal Ion Binding ◽  
Ion Sensing ◽  
Calcium Magnesium ◽  
Ion Binding Sites ◽  
Liver Flukes

Abstract Calmodulin is a calcium ion-sensing signalling protein found in eukaryotics. Two calmodulin-like gene sequences were identified in an EST library from adult liver flukes. One codes for a protein (FhCaM1) homologous to mammalian calmodulins (98% identity), whereas the other protein (FhCaM2) has only 41% identity. These genes were cloned into expression vectors and the recombinant proteins were expressed in Escherichia coli. Gel shift assays showed that both proteins bind to calcium, magnesium and zinc ions. Homology models have been built for both proteins. As expected, FhCaM1 has a highly similar structure to other calmodulins. Although FhCaM2 has a similar fold, its surface charge is higher than FhCaM1. One of the potential metal ion-binding sites has lower metal-ion co-ordination capability, while another has an adjacent lysine residue, both of which may decrease the metal-binding affinity. These differences may reflect a specialised role for FhCaM2 in the liver fluke.

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