scholarly journals Comparative Study of Hicrome Agar Medium with Conventional Culture System for the Isolation of Uropathogens

2018 ◽  
Vol 24 (2) ◽  
pp. 128-135
Author(s):  
S Gul Nahar ◽  
M Bulbul Hasan ◽  
Mst Rokeya Khatun ◽  
M Nawshad Ali
Keyword(s):  
Urine Culture ◽  
Culture Media ◽  
Agar Medium ◽  
Blood Agar ◽  
Macconkey Agar ◽  
Biochemical Tests ◽  
College Hospital ◽  
Conventional Culture ◽  
Presumptive Identification ◽  
Chromogenic Agar

Objective: The present study was done to compare the performance of chromogenic agar medium and conventional culture media for the isolation and presumptive identification of uropathogen.Methodology: A total 300 sample were collected from Rajshahi Medical College Hospital, Bangladesh during January to June, 2008. Urine samples of the suspected UTI cases, showing pus cells >5/HPF on microscopic examination were included for urine culture simultaneously onto 2 conventional media (Blood agar and MacConkey agar) and chromogenic agar medium (HiCrome UTI agar medium). Results: Culture yielded 139 (46.33%) bacterial growth among them, 133 (44.33%) showed single organism and remaining 06 (2.00%) showed mixed growth of two organisms in different combinations. It is evident from the present study that both HiCrome UTI agar and Blood agar (BA) media supported growth of all 145 bacteria, while MacConkey (MAC) agar yielded 133(91.72%) bacterial growths. The rate of presumptive identification of the isolates was found significantly higher (97.24%) on HiCrome UTI agar when compared with the MacConkey agar (80.68%) and Blood agar (27.58%) media. Out of 91 E. coli isolated, 88(96.70%) could be identified differentially on HiCrome UTI agar medium in contrast to 85(93.40%) on MacConkey agar and only 06(06.59%) on Blood agar. Again, all 06 (100%) of the isolate-pairs of mixed growth were identified distinctly on HiCrome UTI agar, whereas both Blood agar and MacConkey agar media could revealed only 01(16.66%) of the polymicrobial growth.Conclusion: HiCrome UTI agar medium has been documented for its very high yielding rate, rapid presumptive identification of both single and polymicrobial growths with greater precision and avoidance of biochemical tests for further identification of uropathogens. Thus it can be recommended as primary urine culture medium to be used by the clinical microbiology laboratories.TAJ 2011; 24(2): 128-135

scholarly journals Chromogenic Agar Medium : A Versatile Tool for the Diagnosis of UTI

2018 ◽  
Vol 25 ◽  
pp. 64-71
Author(s):  
S Gul Nahar ◽  
M Bulbul Hasan ◽  
Mst Rokeya Khatun ◽  
M Nawshad Ali ◽  
DK Mohanta
Keyword(s):  
Urine Culture ◽  
Blood Agar ◽  
Macconkey Agar ◽  
Biochemical Tests ◽  
College Hospital ◽  
Medical College ◽  
Presumptive Identification ◽  
Versatile Tool ◽  
Chromogenic Agar ◽  
Agar Media

Objective: The present study was done on Chromogenic agar media to identify uropathogens more efficiently by its characteristic colony colour for each of the organism.Methodology: A total 300 sample were collected from Rajshahi Medical College Hospital, Bangladesh. Urine samples of the suspected UTI cases, showing pus cells >5/HPF on microscopic examination were included for urine culture simultaneously onto Chromogenic agar media, Blood agar and MacConkey agar media.Results: Culture yielded 139 (46.33%) bacterial growth among them, 133 (44.33%) showed single organism and remaining 06 (2.00%) showed mixed growth of two organisms in different combinations. It is evident from the present study that both Chromogenic agar media and Blood agar (BA) media supported growth of all 145 bacteria, while MacConkey (MAC) agar yielded 133(91.72%) bacterial growths. The rate of presumptive identification of the isolates was found significantly higher (97.24%) on Chromogenic agar media when compared with the MacConkey agar (80.68%) and Blood agar (27.58%) media. Out of 91 E. coli isolated, 88(96.70%) could be identified differentially on Chromogenic agar media in contrast to 85(93.40%) on MacConkey agar and only 06(06.59%) on Blood agar. Again, all 06 (100%) of the isolate-pairs of mixed growth were identified distinctly on Chromogenic agar media, whereas both Blood agar and MacConkey agar media could revealed only 01(16.66%) of the polymicrobial growth.Conclusion: Chromogenic agar media has been documented for its very high yielding rate, rapid presumptive identification of both single and polymicrobial growths with greater precision and avoidance of biochemical tests for further identification of uropathogens. Thus it can be recommended as primary urine culture medium to be used by the clinical microbiology laboratories.TAJ 2012; 25: 64-71

scholarly journals Performance of chromID ESBL, a chromogenic medium for detection of Enterobacteriaceae producing extended-spectrum β-lactamases

2008 ◽  
Vol 57 (3) ◽  
pp. 310-315 ◽  
Author(s):  
Hélène Réglier-Poupet ◽  
Thierry Naas ◽  
Amélie Carrer ◽  
Anne Cady ◽  
Jean-Marie Adam ◽  
...  
Keyword(s):  
Agar Medium ◽  
Klebsiella Oxytoca ◽  
Selective Medium ◽  
Clinical Samples ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
Extended Spectrum ◽  
Presumptive Identification ◽  
Chromogenic Agar ◽  
Synergy Testing

The chromogenic agar medium chromID ESBL (bioMérieux) was compared with BLSE agar medium (AES) for selective isolation and presumptive identification of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae from clinical samples. A total of 765 samples (468 rectal swabs, 255 urine samples and 42 pulmonary aspirations) obtained from 547 patients was processed. All bacterial strains isolated on either medium were further characterized using biochemical tests, and ESBL producers were confirmed by synergy testing. Genetic characterization of ESBL genes was determined by PCR. A total of 33 ESBL-producing Enterobacteriaceae strains [Escherichia coli (n=16), Klebsiella pneumoniae (n=8), Enterobacter spp. (n=3), Citrobacter spp. (n=5) and Proteus mirabilis (n=1)] was recovered. The sensitivity after 24 h incubation was 88 % for chromID ESBL and 85 % for BLSE agar. At 48 h, the sensitivity of chromID ESBL increased to 94 % and was higher than that obtained with BLSE agar. The positive predictive value at 24 h for chromID ESBL was 38.7 % [95 % confidence interval (95 % CI) 28.3 –50.2 %)], which was significantly higher than that for BLSE agar [15.4 %, 95 % CI 10.1 –21.5 %]. On both media, false-positive results were mostly due to Pseudomonas aeruginosa and to Enterobacteriaceae overproducing chromosomal cephalosporinase (Enterobacter spp.) or a chromosomal penicillinase (Klebsiella oxytoca). This study showed that chromID ESBL, a ready-to-use chromogenic selective medium, is sensitive and specific for rapid, presumptive identification of ESBL-producing Enterobacteriaceae. Its chromogenic properties and its selectivity are particularly useful in specimens containing resident associated flora.

scholarly journals Selective and differential medium for isolation of Clostridium difficile

1979 ◽  
Vol 9 (2) ◽  
pp. 214-219 ◽  
Author(s):  
W L George ◽  
V L Sutter ◽  
D Citron ◽  
S M Finegold
Keyword(s):  
Clostridium Difficile ◽  
Antimicrobial Agent ◽  
Rapid Method ◽  
Agar Medium ◽  
Egg Yolk ◽  
Blood Agar ◽  
Fluorescent Properties ◽  
Presumptive Identification ◽  
Differential Medium

Clostridium difficile is a recognized cause of pseudomembranous (antimicrobial agent-associated) colitis and may be one of the causes of antimicrobial agent-induced diarrhea. A selective and differential agar medium that contains cycloserine, cefoxitin, fructose, and egg yolk (CCFA) was developed to facilitate the isolation of C. difficile from fecal specimens. Quantitative cultures of 16 stock strains of C. difficile on this medium (and on a medium containing cycloserine, fructose, and egg yolk) yielded counts equivalent to those obtained on blood agar; other media selective for clostridia, including Clostrisel agar, reinforced clostridial agar plus 0.2% para-cresol, and egg yolk-neomycin agar (the latter was inoculated with cultures subjected to prior heat shocking), were also tested and found to be inhibitory to the growth of C. difficile. Of 28 fecal or colostomy effluent specimens cultured on the above media, 14 yielded C. difficile. CCFA was found to be the most sensitive and selective of these media for the recovery of C. difficile. Colonies of C. difficile growing on CCFA had distinctive morphological and fluorescent properties which were sufficient for presumptive identification. CCFA should provide a rapid method for the screening of fecal specimens from patients with antimicrobial agent-associated diarrhea or colitis for C. difficile.

scholarly journals Use of 2-aminoacetophenone production in identification of Pseudomonas aeruginosa

1979 ◽  
Vol 9 (4) ◽  
pp. 479-484
Author(s):  
C D Cox ◽  
J Parker
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Mass Spectroscopy ◽  
Culture Media ◽  
Agar Medium ◽  
Growth Cycle ◽  
Fluorometric Assay ◽  
Blood Agar ◽  
Burn Wounds ◽  
Diagnostic Importance ◽  
Colorimetric Methods

A grapelike odor is often of diagnostic importance in detecting the growth of Pseudomonas aeruginosa in culture and in burn wounds. The compound responsible for the odor has been identified as 2-aminoacetophenone by mass spectroscopy. Although the grape odor is sometimes difficult to detect in culture media, gas chromatographic, fluorometric, and colorimetric methods can be utilized to assay 2-aminoacetophenone production in a variety of media. Its synthesis occurs relatively early in the growth cycle. It has proved easy and convenient to detect 2-aminoacetophenone excretion by P. aeruginosa after 24 h of incubation on blood agar plates employing a fluorometric assay of ether extracts of the agar medium.

Malonate Dulcitol Lysine Iron Agar—A New Differential Medium for the Identification of Salmonella Subgenera I—III

1972 ◽  
Vol 55 (1) ◽  
pp. 214-218
Author(s):  
John R Stroup
Keyword(s):  
Minimal Medium ◽  
Agar Medium ◽  
Decarboxylase Activity ◽  
Lysine Decarboxylase ◽  
Biochemical Tests ◽  
Presumptive Identification ◽  
Differential Medium

Abstract A new differential medium, malonate dulcitol lysine iron agar, containing malonate, dulcitol, L-lysine, and an H2S indicating system has been developed for use at the triple sugar iron agar stage for the identification of Salmonella subgenera I–III. This medium differentiates the subgenera on the basis of the malonate and dulcitol reactions. L-Lysine is incorporated to confirm lysine decarboxylase activity for dulcitol-positive subgenus I cultures. Since it is a near minimal medium, it i? advantageous to inoculate triple sugar iron agar or lysine iron agar slants in parallel with the new medium to act as a source of cells for “O” group serology and to avoid missing aberrant biochemical types. Although a few atypical reactions were noted, all of the 35 typical Salmonella of subgenera I–III tested were typical on malonate dulcitol lysine iron agar medium. Triple sugar iron agar can be considered as giving a presumptive test for Salmonella. However, as it does not differentiate the subgenera, this distinction must be made when the results of in-depth biochemical tests are known. As the subgenera can be differentiated on the basis of malonate and dulcitol reactions, the new medium should allow for the presumptive identification of Salmonella subgenera at least 24 hr earlier than is now possible with conventional procedures.

scholarly journals Dentistry and Intensive Care Unit: A Brief Report

2021 ◽  
Author(s):  
Lisiane Cristina Bannwart ◽  
Clóvis Lamartine de Moraes Melo Neto ◽  
Daniela Micheline dos Santos ◽  
André Luiz de Melo Moreno ◽  
Aldiéris Alves Pesqueira ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Culture Media ◽  
Blood Agar ◽  
Macconkey Agar ◽  
Candida Spp ◽  
Removable Partial Denture ◽  
Mannitol Salt Agar ◽  
Sabouraud Agar ◽  
Shigella Spp

Abstract Objective The aim of this study is to verify whether removable dentures of patients admitted to an intensive care unit (ICU) are niches of microorganisms that can cause pathologies (Staphylococcus aureus, Candida spp., and enterobacteria). Materials and Methods Fifteen patients who were denture wearers (removable partial denture and complete denture) were included in this study. Patients must wear their dentures daily, and these dentures must have acrylic parts. Microbial biofilm was collected from the acrylic part of one denture of each patient. Then, the biofilm was seeded on different culture media: Sabouraud agar, blood agar, MacConkey agar, and mannitol salt agar. In this study, biochemical evaluations of microorganisms were performed. Statistical analysis The percentage of dentures with the microorganism identified by each culture medium was calculated. Results In total, 100% of the dentures were positive for Staphylococcus spp. (blood agar) and Candida spp. (Sabouraud agar); 33.3% of the dentures were positive for S. aureus (Mannitol salt agar); and 13.3% of the dentures were positive for Shigella spp. (MacConkey agar). Conclusion Removable dentures of patients (removable partial dentures and complete dentures) admitted to an ICU are niches of microorganisms that can cause pathologies.

A SEARCH FOR ASSOCIATION BETWEEN BIOFILM STRENGTH AND SITES OF INFECTION CAUSED BY ACINETOBACTER BAUMANNII COMPLEX

2021 ◽  
pp. 7-9
Author(s):  
Sudeb Roy ◽  
Moupiya Nandi ◽  
Debarshi Jana
Keyword(s):  
Acinetobacter Baumannii ◽  
Culture Media ◽  
Clinical Sample ◽  
Venous Catheter ◽  
Blood Stream ◽  
Multidrug Resistant ◽  
Blood Agar ◽  
Clinical Samples ◽  
Macconkey Agar ◽  
Eskape Pathogens

INTRODUCTION: In the world of infectious diseases, a group of bacteria known as ESKAPE pathogens, are responsible for most of the life threatening multidrug resistant (MDR) and extensively drug resistant (XDR) infections worldwide. The ESKAPE pathogens comprise of Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter spp. AIMS AND OBJECTIVES: The objective of this study is isolate and identify Acinetobactor baumannii complex from clinical samples. Measure the biolm forming capacity of the isolates. Possible association of this biolm strength with the type of clinical sample from which they are isolated. MATERIALS AND METHODS: The samples which were tested in the laboratory were the following: Sputum, End tracheal tube aspirates, Pus, Urine, Blood for culture, Central venous catheter tips & Cerebrospinal uid. Following culture media were taken: MaCconkey agar & Blood agar. The colonies suggestive of Acinetobacter baumannii were selected. On MaCconkey agar, non- pigmented NLF colonies with pink to light lavender hue; and on blood agar, opaque non-hemolytic colonies were obtained. Gram stains from selected colony showed gram negative coccobacilli. Hanging drop preparation showed non-motile bacteria. RESULTS AND ANALYSIS: We found maximum number of strong biolm producers were found among the isolates causing infection of CVC tips (70%). This was closely followed by ETtube isolates (69.5%). The isolates from blood stream, urine, and sputum samples showed all the three patterns, namely, no biolm producer, moderate biolm producer, and strong biolm producer. Our study showed interestingly, no strong biolm producer was found from pus samples, whereas from the CSF isolates no non-producer of biolm emerged. CONCLUSION: This study centers around strength or pattern of biolm formation by this bacteria. It was interesting to nd that there was a statistically signicant association between patterns of biolm produced by Acinetobacter baumannii and the various samples from which they are isolated. This made us to hint at a probable relation between biolm patterns and organotropism as an hypothesis. Only further investigations will tell if it can stand the test of time.

scholarly journals Applications and integration of chromogenic culture media in clinical microbiology

2010 ◽  
Vol 31 (3) ◽  
pp. 127
Author(s):  
John Merlino
Keyword(s):  
Culture Media ◽  
Effective Means ◽  
Traditional Culture ◽  
Cost Effective ◽  
Conventional Culture ◽  
Chromogenic Agar ◽  
Isolation Media ◽  
Species Specific ◽  
Micro Organisms ◽  
New Generation

The ability to rapidly detect and identify micro-organisms is of paramount importance for many microbiology laboratories. The application of new classes of enzymatic-based chromogenic compounds has revolutionised traditional culture media, leading to the developments of a new generation of chromogenic media. Many studies have shown that the chromogenic agar has become more than an isolation media when compared to conventional culture media. A newer, faster, more cost-effective means in the presumptive identification or screening of microorganisms which are either genus, or in some cases species-specific, and allows superior differentiation of mixed micro-organisms in cultures. When supplemented with antibiotics or other agents, chromogenic media allows the screening for multidrug resistance and virulence in many micro-organisms and can be easily integrated with other automated and/or molecular-based methods.

Sign in / Sign up

Export Citation Format

Share Document