scholarly journals Efficiency of Bacillus subtilis Bs10 as a plant growth promoting inoculant in soybean crop under field conditions

2021 ◽  
Vol 10 (14) ◽  
pp. e441101422141
Author(s):  
Aloisio Freitas Chagas Junior ◽  
Lillian França Borges Chagas ◽  
Albert Lennon Lima Martins ◽  
Brigitte Sthepani Orozco Colonia ◽  
Manuella Costa Souza ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Field Experiments ◽  
Field Conditions ◽  
Control Treatment ◽  
Soybean Crop ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Absolute Control ◽  
Positive Results

The growth of the Brazilian market for biological defensive follows a worldwide trend of reducing the use of pesticides in crops, due to international and societal demands for a more sustainable agriculture. The objective of the present work was to evaluate the efficiency of Bacillus subtilis Bs10 as an inoculant for the soybean crop, through the promotion of plant growth and productive performance in the field. Three field experiments were conduct in the municipalities of Porto Nacional and Formoso do Araguaia, Tocantins, Brazil. The treatments used in the experiments were five doses of the B. subtilis Bs10-based product (0, 100, 200, 300 and 400 mL 50 kg of seeds-1), plus one treatment with a commercial B. subtilis-based product. The inoculation of B. subtilis Bs10 provided increases in biomass, agronomic characteristics, plant population and productivity in soybeans under field conditions. There were positive results starting at a dose of 200 mL, with gains in productivity ranging from 28 to 41% in relation to the absolute control treatment, without inoculation of B. subtilis, and from 11 to 42% in relation to the treatment with commercial product. The bio formulated B. subtilis Bs10 should be recommend as a plant growth promoting inoculant in soybean.

scholarly journals IMPACT OF PLANT GROWTH PROMOTING RHIZOBACTERIA AS BIO-CONTROL AGENTS ON CITRUS NEMATODE, TYLENCHULUS SEMIPENETRANS INFECTING BALADY ORANGE (CITRUS SINENSIS L.) AND IMPROVING ITS PRODUCTIVITY

2019 ◽  
Keyword(s):  
Humic Acid ◽  
Plant Growth ◽  
Citrus Sinensis ◽  
Field Experiments ◽  
Plant Growth Promoting Rhizobacteria ◽  
Control Treatment ◽  
Tylenchulus Semipenetrans ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Citrus Nematode

Three bacterial species of plant growth promoting rhizobacteria (PGPR) namely Paenibacillus polymyxa, Methylobacterium mesophilicum and Methylobacterium radiotolerans were tested alone or combined with humic acid as bio-control agents against the citrus nematode Tylenchulus semipenetrans under laboratory and field conditions. Results cleared that; all tested PGPR species produced IAA, HCN, ammonia, chitinase and protease enzymes and also solubilized phosphate in laboratory. P. polymyxa emphasized the superiority among other species in all PGPR properties except for phosphate solubilization, whereas M. radiotolerans showed highest amount of phosphorus solubilized in culture media. On the other hand, the results of the nematode survey conducted on orange, Citrus sinensis L. cv Balady grown in different localities of Ismailia and Sharkia Governorates during season 2019, revealed the presence of seven genera and species of plant-parasitic nematodes. Among which, T. semipenetrans occurred in all examined samples (100% frequency of occurrence) with a relatively high population density of 2330 and 2640 infected juveniles (J2) /250 g soil in Ismailia and Sharkia Governorates, respectively. Field experiments were conducted in two different locations, at Ismailia and Sharkia Governorates to assess the effectiveness of PGPR strains alone or combined to reduce the numbers of T. semipenetrans during season 2019. It was found that, all treatments caused significant (P≤0.05) reduction in T. semipenetrans population, compared to control treatment. The nematicide, Nemathorin® 10% G followed by P. polymyxa (20 L.fed-1 ) + foliarspraying of M. mesophilicm (5 L.fed-1 ) gave the highest efficacy in controlling the citrus nematode. Percentage reduction in numbers of J2/250g soil and adult females/1g roots for these treatments in Ismailia Governorate were 91% (85.7%) and 91.4% (89.5%), respectively. While the parallel values in Sharkia Governorate were 90% (87%) and 94% (90%), respectively. The combination of P. polymyxa and humic acid (20 L.fed-1 ) with foliar spraying of M. mesophilicm (5 L.fed-1 ) gained the third position. All treatments increased the fruit yield compared to control treatment. The highest percentages of increase were determined with Nemathorin (160% and 206%) followed by P. polymyxa + Humic acid (20 L.fed-1 ) + foliar spraying of M. mesophilicum (155.7% and 193%) and M. radiotolerans + P. polymyxa + foliar spraying of M. mesophilicum (153% and182%) in Ismailia, and Sharkia Governorates respectively.

scholarly journals Influence of plant growth-promoting rhizobacteria (PGPR) on blackberry (Rubus glaucus Benth. cv. thornless) growth under semi-cover and field conditions

2018 ◽  
Vol 67 (2) ◽  
pp. 258-263 ◽  
Author(s):  
Jacobo Robledo-Buriticá ◽  
Juan Carlos Aristizábal-Loaiza ◽  
Nelson Ceballos-Aguirre ◽  
Teresa Cabra-Cendales
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Field Conditions ◽  
El Sistema ◽  
Plant Growth Promoting ◽  
Growth Promoting

Este estudio muestra el efecto de tres cepas de rizobacterias promotoras del crecimiento vegetal (PGPR) del género Bacillus sobre el desarrollo de la mora (Rubus glaucus Benth) bajo condiciones de semitecho y libre exposición (sistemas de cultivo). La cepa endógena Bacillus subtilis GIBI-200, B. pumilus GIBI-206, su mezcla (B. subtilis GIBI-200 + B. pumilus GIBI-206) y la cepa comercial B. subtilis QTS-713 se compararon con la fertilización mineral convencional (Control) en cada sistema de cultivo. El sistema a libre exposición presentó valores más altos (P <0.001) que el semitecho en: número total de ramas (7.32), número de ramas productivas (7.0), flores por racimo (26.2) y menor porcentaje de ramas improductivas (6.1%). Se obtuvo diferencias significativas (P <0.05) en el número total de ramas a través del tiempo como efecto las cepas bacterianas. No obstante, la última observación de esta variable no presentó diferencias estadísticas entre cepas y fertilización mineral. No se observaron diferencias significativas en términos de número, longitud, diámetro y flores en las ramas productivas como efecto de las cepas bacterianas y el Control. B. subtilis GIBI-200 + B. pumilus GIBI-206 mostró un comportamiento estadístico similar comparado con la fertilización mineral en el número de racimos (8.3) y porcentaje de ramas improductivas (11.1%). Además, GIBI-200 + GIBI-206, a pesar de la igualdad estadística, expuso mayores valores que las cepas individuales. El sistema a libre exposición sigue siendo la alternativa más prometedora para el desarrollo de cultivos de mora y PGPR actúan como biofertilizantes permitiendo lograr efectos en el crecimiento a largo plazo de mora similar a la fertilización mineral. Las mezclas de PGPR pueden producir un efecto sinérgico y nuevas combinaciones deben ser evaluadas en futuros estudios.

Aplikasi Bacillus subtilis pada beberapa Bahan Organik terhadap Pertumbuhan dan Produksi Tanaman Cabai Rawit (Capsicum frutescens L.)

2020 ◽  
Vol 21 (1) ◽  
pp. 14-19
Author(s):  
Praptiningsih Gamawati Adinurani ◽  
Sri Rahayu ◽  
Nurul Fima Zahroh
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Capsicum Frutescens ◽  
Plant Growth Promoting ◽  
Growth Promoting

Mikroba Bacillus subtilis merupakan agen pengendali hayati mempunyai kelebihan sebagai Plant Growth Promoting Rhizobacteria (PGPR) yaitu dapat berfungsi sebagai biofertilizer, biostimulan, biodekomposer dan bioprotektan. Tujuan penelitian mengetahui potensi B. subtilis dalam merombak bahan organik sebagai usaha meningkatkan ketersediaan bahan organik tanah yang semakin menurun. Penelitian menggunakan Rancangan Petak Terbagi dengan berbagai  bahan organik sebagai petak utama (B0 = tanpa bahan organik, B1 = kotoran ayam,  B2 = kotoran kambing, B3 = kotoran sapi) dan aplikasi B.subtilis sebagai anak petak (A0 = 0 cc/L, A1 = 5cc/L, A2 = 10 cc/L, Pengamatan meliputi variabel tinggi tanaman, indeks luas daun, jumlah buah per tanaman, berat buah per tanaman, dan bahan organik tanah. Data pengamatan  dianalisis ragam  menggunakan  Statistical Product and Service Solutions (SPSS) versi 25 dan dilanjutkan dengan uji Duncan untuk mengetahui signifikansi perbedaan antar perlakuan. Hasil penelitian menunjukkan tidak terdapat interaksi antara bahan organik kotoran ternak dan konsentrasi B. subtilis terhadap semua variabel pengamatan. Potensi B. subtilis sangat baik dalam mendekomposisi bahan organik yang ditunjukkan dengan peningkatan bahan organik, dan hasil terbaik pada kotoran  sapi (B3) dan konsentrasi B. subtilis 15 mL/L masing-masing sebesar 46.47 % dan 34.76 %. Variabel pertumbuhan tidak berbeda nyata kecuali tinggi tanaman dengan pertambahan tinggi paling banyak pada pemberian kotoran kambing sebesar 170.69 %.

scholarly journals Foliar-Applied Potassium Silicate Coupled with Plant Growth-Promoting Rhizobacteria Improves Growth, Physiology, Nutrient Uptake and Productivity of Faba Bean (Vicia faba L.) Irrigated with Saline Water in Salt-Affected Soil

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 894
Author(s):  
Emad M. Hafez ◽  
Hany S. Osman ◽  
Usama A. Abd El-Razek ◽  
Mohssen Elbagory ◽  
Alaa El-Dein Omara ◽  
...  
Keyword(s):  
Plant Growth ◽  
Fresh Water ◽  
Faba Bean ◽  
Saline Water ◽  
Plant Growth Promoting Rhizobacteria ◽  
Control Treatment ◽  
Combined Application ◽  
Bean Plants ◽  
Plant Growth Promoting ◽  
Growth Promoting

The continuity of traditional planting systems in the last few decades has encountered its most significant challenge in the harsh changes in the global climate, leading to frustration in the plant growth and productivity, especially in the arid and semi-arid regions cultivated with moderate or sensitive crops to abiotic stresses. Faba bean, like most legume crops, is considered a moderately sensitive crop to saline soil and/or saline water. In this connection, a field experiment was conducted during the successive winter seasons 2018/2019 and 2019/2020 in a salt-affected soil to explore the combined effects of plant growth-promoting rhizobacteria (PGPR) and potassium (K) silicate on maintaining the soil quality, performance, and productivity of faba bean plants irrigated with either fresh water or saline water. Our findings indicated that the coupled use of PGPR and K silicate under the saline water irrigation treatment had the capability to reduce the levels of exchangeable sodium percentage (ESP) in the soil and to promote the activity of some soil enzymes (urease and dehydrogenase), which recorded nearly non-significant differences compared with fresh water (control) treatment, leading to reinstating the soil quality. Consequently, under salinity stress, the combined application motivated the faba bean vegetative growth, e.g., root length and nodulation, which reinstated the K+/Na+ ions homeostasis, leading to the lessening or equalizing of the activity level of enzymatic antioxidants (CAT, POD, and SOD) compared with the controls of both saline water and fresh water treatments, respectively. Although the irrigation with saline water significantly increased the osmolytes concentration (free amino acids and proline) in faba bean plants compared with fresh water treatment, application of PGPR or K-silicate notably reduced the osmolyte levels below the control treatment, either under stress or non-stress conditions. On the contrary, the concentrations of soluble assimilates (total soluble proteins and total soluble sugars) recorded pronounced increases under tested treatments, which enriched the plant growth, the nutrients (N, P, and K) uptake and translocation to the sink organs, which lastly improved the yield attributes (number of pods plant−1, number of seeds pod−1, 100-seed weight). It was concluded that the combined application of PGPR and K-silicate is considered a profitable strategy that is able to alleviate the harmful impact of salt stress alongside increasing plant growth and productivity.

Effects of humic acid and plant growth-promoting rhizobacteria (PGPR) on induced resistance of canola to Brevicoryne brassicae L

2018 ◽  
Vol 109 (4) ◽  
pp. 479-489 ◽  
Author(s):  
R. Sattari Nasab ◽  
M. Pahlavan Yali ◽  
M. Bozorg-Amirkalaee
Keyword(s):  
Humic Acid ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Brevicoryne Brassicae ◽  
Control Treatment ◽  
Net Reproductive Rate ◽  
Rate Of Increase ◽  
Significant Difference ◽  
Plant Growth Promoting ◽  
Growth Promoting

AbstractThe cabbage aphid, Brevicoryne brassicae L. (Hem: Aphididae), is an important pest of canola that can considerably limit profitable crop production either through direct feeding or via transmission of plant pathogenic viruses. One of the most effective approaches of pest control is the use of biostimulants. In this study, the effects of humic acid, plant growth-promoting rhizobacteria (PGPR), and integrated application of both compounds were investigated on life table parameters of B. brassicae, and the tolerance of canola to this pest. B. brassicae reared on plants treated with these compounds had the lower longevity, fecundity, and reproductive period compared with control treatment. The intrinsic rate of natural increase (r) and finite rate of increase (λ) were lowest on PGPR treatment (0.181 ± 0.004 day−1 and 1.198 ± 0.004 day−1, respectively) and highest on control (0.202 ± 0.005 day−1 and 1.224 ± 0.006 day−1, respectively). The net reproductive rate (R0) under treatments of humic acid, PGPR and humic acid + PGPR was lower than control. There was no significant difference in generation time (T) of B. brassicae among the tested treatments. In the tolerance test, plants treated with PGPR alone or in integrated with humic acid had the highest tolerance against B. brassicae. The highest values of total phenol, flavonoids, and glucosinolates were observed in treatments of PGPR and humic acid + PGPR. Basing on the antibiosis and tolerance analyses in this study, we concluded that canola plants treated with PGPR are more resistant to B. brassicae. These findings could be useful for integrated pest management of B. brassicae in canola fields.

scholarly journals Pengaruh Aplikasi Plant Growth Promoting Rhizobacteria terhadap Pertumbuhan dan Hasil Tanaman Bawang Merah (Allium cepa L. Aggregatum group)

Vegetalika ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 512
Author(s):  
Nanda Dwi Hafri ◽  
Endang Sulistyaningsih ◽  
Arif Wibowo
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Allium Cepa ◽  
Burkholderia Cepacia ◽  
Plant Growth Promoting Rhizobacteria ◽  
Bacillus Methylotrophicus ◽  
Plant Growth Promoting ◽  
Growth Promoting

Salah satu upaya penanganan penyakit moler bawang merah dilakukan melalui aplikasi Trichoderma. Aplikasi Trichoderma pada bawang merah memiliki beberapa keunggulan, yaitu mampu mensintesis hormon pertumbuhan tanaman. Terdapat jenis mikroba lain yang juga mampu meningkatkan fitohormon pada tanaman, yaitu Plant Growth Promoting Rhizobacteria (PGPR). Tujuan dari penelitian ini adalah untuk mengetahui dan menentukan isolat PGPR yang memiliki pengaruh paling baik terhadap pertumbuhan dan hasil bawang merah varietas Crok Kuning di lahan sawah. Penelitian ini menggunakan Rancangan Acak Kelompok Lengkap (RAKL) faktor tunggal dengan tiga blok sebagai ulangan. Faktor perlakuan yang digunakan adalah lima isolat PGPR, yaitu: Bp.25.7 Bacillus subtilis, BrSG.5 Bacillus amyloliquofaciens, Bp.25.2 Bacillus methylotrophicus, BrsM.4 Burkholderia cepacia, danBp.25.6 Bacillus amyloliquofaciens dengan dua kontrol, yaitu kontrol positif berupa Trichoderma dan kontrol negatif tanpa aplikasi perlakuan. Hasil penelitian menunjukkan bahwa pemberian perlakuan isolat Bp.25.2 Bacillus methylotrophicus pada bawang merah menyebabkan Laju Asimilasi Bersih (LAB) bawang merah lebih tinggi dibandingkan dengan pemberian perlakuan empat isolat PGPR lainnya maupun kontrol, tetapi sama baiknya dengan pemberian perlakuan Trichoderma. LAB yang tinggi menyebabkan Laju Pertumbuhan Tanaman (LPT) bawang merah dengan pemberian perlakuan Bp.25.2 Bacillus methylotrophicus yang lebih tinggi dibandingkan dengan BrSG.5 Bacillus amyloliquofaciens dan Bp.25.6 Bacillus amyloliquofaciens, tetapi sama baiknya dengan pemberian perlakuan dua isolat PGPR lainnya, kontrol, maupun Trichoderma. Pemberian aplikasi lima isolat PGPR sama baiknya dengan aplikasi Trichoderma dalam meningkatkan pertumbuhan tanaman bawang merah pada variabel luas permukaan daun dan bobot kering total dibandingkan kontrol. Peningkatan variabel pertumbuhan ini tidak diikuti oleh peningkatan variabel hasil dan produktivitas bawang merah tidak berbeda nyata antar perlakuan.

scholarly journals Quorum quenching activity of the PGPR Bacillus subtilis UD1022 alters nodulation efficiency of Sinorhizobium meliloti on Medicago truncatula

2020 ◽  
Author(s):  
Amanda Rosier ◽  
Pascale B. Beauregard ◽  
Harsh P. Bais
Keyword(s):  
Bacillus Subtilis ◽  
Plant Growth ◽  
Medicago Truncatula ◽  
Sinorhizobium Meliloti ◽  
Quorum Quenching ◽  
Agricultural System ◽  
Synergistic Activity ◽  
Sustainable Solutions ◽  
Plant Growth Promoting ◽  
Growth Promoting

AbstractPlant growth promoting rhizobacteria (PGPR) have enormous potential for solving some of the myriad challenges facing our global agricultural system. Intense research efforts are rapidly moving the field forward and illuminating the wide diversity of bacteria and their plant beneficial activities. In the development of better crop solutions using these PGPR, producers are including multiple different species of PGPR in their formulations in a ‘consortia’ approach. While the intention is to emulate more natural rhizomicrobiome systems, the aspect of bacterial interactions has not been properly regarded. By using a tri-trophic model of Medicago truncatula A17 Jemalong, its nitrogen (N)-fixing symbiont Sinorhizobium meliloti Rm8530 and the PGPR Bacillus subtilis UD1022, we demonstrate indirect influences between the bacteria affecting their plant growth promoting activities. Co-cultures of UD1022 with Rm8530 significantly reduced Rm8530 biofilm formation and downregulated quorum sensing (QS) genes responsible for symbiotically active biofilm production. This work also identifies the presence and activity of a quorum quenching lactonase in UD1022 and proposes this as the mechanism for non-synergistic activity of this model ‘consortium’. These interspecies interactions may be common in the rhizosphere and are critical to understand as we seek to develop new sustainable solutions in agriculture.

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