scholarly journals Influence of carbon source, agitation and aeration rates for production yeast biomass which potential of use for biological control

2020 ◽  
Vol 9 (4) ◽  
pp. e174943066
Author(s):  
Ana Paula Colares de Andrade ◽  
Helder Levi da Silva ◽  
Gustavo Adolfo Saavedra Pinto
Keyword(s):  
Biological Control ◽  
Carbon Source ◽  
Invert Sugar ◽  
Aeration Rate ◽  
Tropical Fruits ◽  
Bioreactor Cultivation ◽  
Yeast Biomass ◽  
Natural Microflora ◽  
Orbital Shaker ◽  
Agitation And Aeration

The fermentation processes are used to promote better results when it is desired to increase the biomass of organisms that can be used in biological control. Thus, the aim of this work was to expand the scale of production of biomass of yeasts that have potential for biological control. A total of five strains of yeasts isolated from natural microflora of tropical fruits, a strain was selected and evaluated for the influence of the carbon source, cultivation orbital shaker and bioreactor. The tests performed demonstrate that the use of invert sugar as carbon source favored production of biomass material when compared to that obtained with glucose. The bioreactor cultivation of the yeast strain has enhanced performance, since it favors an increase of 24.12% (6.47 to 8.03 g.L-1) in biomass production when compared to the fermentation orbital shaker and that may be associated with increased agitation and aeration rate.

scholarly journals Yeast biomass production with potential for biological control: process strategies for increasing yield

2020 ◽  
Vol 9 (4) ◽  
pp. e169943057
Author(s):  
Ana Paula Colares de Andrade ◽  
Helder Levi da Silva ◽  
Gustavo Adolfo Saavedra Pinto
Keyword(s):  
Biological Control ◽  
Carbon Source ◽  
Biomass Production ◽  
Batch Process ◽  
Aeration Rate ◽  
Total Biomass ◽  
Fed Batch ◽  
Promising Alternative ◽  
Yeast Biomass ◽  
Feed Medium

The quality of vegetable products is directly linked to the techniques used in the field, in order to ensure safe and healthy products to health. In this context, the use of yeasts with potential for biological control proved to be a promising alternative to assure the safety of these foods. Fermentation processes have been used to promote the development of many products, including the production of yeast biomass. The objective of this work was to verify the influence of the aeration rate and the fed batch process in the production of yeast biomass. A yeast strain with biological control potential, belonging to Embrapa's Semi-Arid crop collection, was subjected to simple batch cultivation and fed with different aeration rates (3, 4, 6 and 8 L.ar/min) and concentration of carbon source in the feed medium (200, 400 and 600 g/L). The highest biomass (6.99 g/L) after 24 hours of fermentation was observed in the experiment that used an aeration rate of 8 L.ar/min. Regarding the concentration of the carbon source in the feed medium, it was found that the concentration of 200 g/L favored a greater total biomass (11.21 g/L) and reduced the production of ethanol (0.65 g/L ), while the concentration of 600 g/L favored less biomass production (7.90 g/L) and higher ethanol production (9.26 g/L). Thus, it was found that the aeration rate and the fed batch process favor the fermentation strategy, as they contribute to the production of yeast biomass and the overall yield of the process.

scholarly journals Lactic acid production ability of Lactobacillus sp. from four tropical fruits using their by-products as carbon source

Heliyon ◽  
2021 ◽  
Vol 7 (5) ◽  
pp. e07079
Author(s):  
Joel Romial Ngouénam ◽  
Chancel Hector Momo Kenfack ◽  
Edith Marius Foko Kouam ◽  
Pierre Marie Kaktcham ◽  
Rukesh Maharjan ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Carbon Source ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Tropical Fruits ◽  
By Products

scholarly journals Production of Enriched Sporidiobolus sp. Yeast Biomass Cultivated on Mixed Coffee Hydrolyzate and Fat/Oil Waste Materials

2021 ◽  
Vol 9 (9) ◽  
pp. 1848
Author(s):  
Martin Szotkowski ◽  
Jiří Holub ◽  
Samuel Šimanský ◽  
Klára Hubačová ◽  
Dagmar Hladká ◽  
...  
Keyword(s):  
Food Industry ◽  
Hydrothermal Carbonization ◽  
Bioreactor Cultivation ◽  
Yeast Biomass ◽  
Yeast Strains ◽  
Coffee Grounds ◽  
Sporidiobolus Pararoseus ◽  
Circular Economics ◽  
Coffee Oil ◽  
Waste Fat

One of the most addressed topics today is the transfer from a linear model of economics to a model of circular economics. It is a discipline that seeks to eliminate waste produced by various industries. The food industry generates huge amounts of waste worldwide, particularly the coffee industry, and related industries produce millions of tons of waste a year. These wastes have potential utility in biotechnology, and in the production of energy, fuels, fertilizers and nutrients, using green techniques such as anaerobic digestion, co-digestion, composting, enzymatic action, and ultrasonic and hydrothermal carbonization. This work is focused on the biotechnological use of processed spent coffee grounds (SCG) and waste fat/oil materials by some Sporidiobolus sp. carotenogenic yeasts in the model of circular economics. The results show that selected yeast strains are able to grow on SCG hydrolysate and are resistant to antimicrobial compounds present in media. The most productive strain Sporidiobolus pararoseus CCY19-9-6 was chosen for bioreactor cultivation in media with a mixture of coffee lignocellulose fraction and some fat wastes. Sporidiobolus pararoseus CCY19-9-6 was able to produce more than 22 g/L of biomass in mixture of SCG hydrolysate and both coffee oil and frying oil. The combined waste substrates induced the production of lipidic metabolites, whereby the production of carotenoids exceeded 5 mg/g of dry biomass. On media with coffee oil, this strain produced high amounts of ubiquinone (8.265 ± 1.648 mg/g) and ergosterol (13.485 ± 1.275 mg/g). Overall, the results prove that a combination of waste substrates is a promising option for the production of carotenoid- and lipid-enriched yeast biomass.

scholarly journals Assessment of the Importance of Similarity in Carbon Source Utilization Profiles between the Biological Control Agent and the Pathogen in Biological Control of Bacterial Speck of Tomato

2002 ◽  
Vol 68 (9) ◽  
pp. 4383-4389 ◽  
Author(s):  
Pingsheng Ji ◽  
Mark Wilson
Keyword(s):  
Biological Control ◽  
Carbon Source ◽  
Disease Severity ◽  
Pseudomonas Syringae ◽  
Biological Control Agent ◽  
Carbon Sources ◽  
Control Agent ◽  
Carbon Source Utilization ◽  
Bacterial Speck

ABSTRACT Bacterial speck of tomato, caused by Pseudomonas syringae pv. tomato, was used to determine whether similarity in carbon source utilization between a preemptive biological control agent and the pathogen was significant in determining the ability of the bacterium to suppress disease. Similarity in carbon source utilization was quantified as the ratio of the number of tomato carbon sources utilized in vitro by the biological control agent to the number of tomato carbon sources utilized in vitro by the target pathogen (the niche overlap index [NOI]). Suppression of the disease was quantified as the percent reduction in disease severity compared to the pathogen-only control when nonpathogenic bacteria were applied to foliage 48 h prior to the pathogen. In the collection of 36 nonpathogenic bacterial strains, there was a significant (P < 0.01), but weak (r2 = 0.25), correlation between reduction in disease severity and similarity in carbon source utilization, suggesting that similarity in carbon source use was significant in determining ability to suppress disease. The relationship was investigated further using catabolic mutants of P. syringae strain TLP2, an effective biological control agent of speck. Catabolic mutants exhibited lower levels of similarity (NOI = 0.07 to 0.90) than did wild-type TLP2 (NOI = 0.93). With these catabolic mutants there was a significant (P < 0.01), and stronger (r2 = 0.42), correlation between reduction in disease severity and similarity in carbon source utilization. This suggests that similarity in carbon source utilization was a more important component of biological control ability for the catabolic mutants than for the nonpathogenic bacteria. Together, these studies indicate that suppression of bacterial speck of tomato was correlated with nutritional similarity between the pathogenic and nonpathogenic bacteria and suggest that preemptive utilization of carbon sources was probably involved in the biological control of the disease by both the naturally occurring nonpathogenic bacteria and the catabolic mutants.

scholarly journals EFFECTS OF WALL EFFECT AND DISSOLVE OXYGEN ON SCALE-UP OF LETTUCE SUSPENSION CULTURE

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 695b-695
Author(s):  
Whei-Lan Teng ◽  
Chiao. Po. Lin
Keyword(s):  
Dissolved Oxygen ◽  
Suspension Culture ◽  
Dissolve Oxygen ◽  
Scale Up ◽  
Aeration Rate ◽  
Plantlet Regeneration ◽  
Wall Effect ◽  
Air Bubble ◽  
The Relationship ◽  
Agitation And Aeration

This study was initiated to solve the problem of wall effect and dissolved oxygen (DO) encountered during establishing a scale-up process of lettuce suspension culture in a fermentor. The wall effect could result in as high as 10% loss of inoculum within 24 hrs. The main reason causing wall effect was identified as foam formed from the interaction of air bubble and inoculum. Bubble isolation by a screen column could successfully solve the wall effect. The effect of dissolved oxygen was then investigated. The plantlet regeneration was increased as dissolved oxygen increased from 20% to 80%. The relationship among agitation and aeration rate as well as oxygen concentration in the air was discussed.

Effect of the Energy and Carbon Source Limitations and Ferric Inhibition on Metabolic Parameters of Leptospirillum Ferrooxidans Growing in Chemostat

2009 ◽  
Vol 71-73 ◽  
pp. 267-270 ◽  
Author(s):  
Marcela Bastías ◽  
J.C. Gentina
Keyword(s):  
Energy Source ◽  
Carbon Source ◽  
Maximum Yield ◽  
Culture Conditions ◽  
Aeration Rate ◽  
The Other ◽  
Metabolic Parameters ◽  
Specific Rate ◽  
Agitation Rate ◽  
Limiting Nutrient

L. ferrooxidans plays a significant role in bioleaching process of ores. Being a chemoautotrophic bacterium, its sources of carbon and energy are independent. In this study were measured separately in a chemostat, the effect of growth limited by each source (CO2 and Fe(II)), and under conditions of inhibition (Fe(III)) on metabolic parameters of the cell. The runs were carried out in a bioreactor with 1.25 liter of KJ culture medium at 33.5 °C, pH 1.8, agitation rate of 300 rpm and aeration rate of 2 VVM. Using only air as CO2 source, it was established that the cells suffer simultaneous limitation of carbon and energy. It was determined that these limitations are released separately when enriching the air with 4% CO2 in one case and when doubling concentration of Fe(II) in the feed stream in the other. Under double limitation maximum yield (YºX/S) and maintenance coefficient (m) were 6.0•10-3 gcel/gFe(II) and 2.48 gFe(III)/gcel•h respectively. Growth limited only by carbon source and only by energy source gave YºX/S 11.5•10-3 and 21.9•10-3 gcel/gFe(II), whereas m was 1.23 and 0.11 gFe(II)/gcel•h respectively. On the other hand, the lower specific Fe(III) production rate (qP) was obtained when cell growth was limited by the energy source and the higher value was observed during growth in presence of 15 g/L exogenous Fe(III). The qP values at D = 0.04 h-1 were 1.93 and 14.04 gFe(III)/gcel•h respectively. In general, the worse the culture conditions the highest the specific rate of Fe(III) production. The bacterium varied its metabolic parameters quite broadly depending on the growth limiting nutrient and presence of Fe(III).

BIOLOGICAL CONTROL OF PESTS OF TROPICAL FRUITS AND NUTS

1976 ◽  
pp. 359-395 ◽  
Author(s):  
F.D. Bennett ◽  
David Rosen ◽  
P. Cochereau ◽  
B.J. Wood
Keyword(s):  
Biological Control ◽  
Tropical Fruits

scholarly journals Development of a Cost Effective Medium for Enhanced Production of Bacillus thuringiensis ?-endotoxin

2016 ◽  
pp. 1-6 ◽  
Author(s):  
Anamika Bhowmik ◽  
Muntahi Mourin ◽  
Md Asaduzzaman Shishir ◽  
Shakila Nargis Khan ◽  
Md Mozammel Hoq
Keyword(s):  
Bacillus Thuringiensis ◽  
Sea Water ◽  
Raw Materials ◽  
Cost Effective ◽  
Luria Bertani ◽  
Bioreactor Cultivation ◽  
Enhanced Production ◽  
Soybean Extract ◽  
Basal Salts ◽  
Agitation And Aeration

The present study was carried out to develop a sustainable production medium using locally available cheap raw materials for biopesticide production by Bacillus thuringiensis subsp. kurstaki (Btk) HD-73. In submerged fermentation (SmF) condition, the conventional Luria-Bertani (LB) medium which was enriched with nitrogen source (10% defatted soybean meal) supported 28.57% sporulation and 125% endotoxin increase over LB (alone). The effect of cystine on sporulation and endotoxin synthesis was highly pronounced in LB-soybean medium (LBS) with a range of 19.54% and 131.35% higher endotoxin yield respectively in SmF condition. While basal salts supplemented in soybean-cystine (SMc) medium, it resulted in 7.65% endotoxin yield compared to LB-soybean-cystine (LBSc) medium. Addition of molasses balanced the C: N ratio in the SMc medium thus helping 84.85% higher endotoxin synthesis after 24 hours fermentation. Substitution of basal salts with cost effective sea water yielded about 19.27% less endotoxin. The optimum medium thus obtained consisting of soybean extract-molasses-cystine with sea water was used in 3.0 L bioreactor cultivation for endotoxin synthesis by Btk HD-73 under 30% saturation of dO2 through cascade of agitation and aeration. The production rate obtained was 1.67 fold higher in bioreactor than in shake flask culture.Bangladesh J Microbiol, Volume 32, Number 1-2,June-Dec 2015, pp 1-6

Are biological control agents, isolated from tropical fruits, harmless to potential consumers?

2017 ◽  
Vol 109 ◽  
pp. 1055-1062 ◽  
Author(s):  
Iris Betsabee Ocampo-Suarez ◽  
Zaira López ◽  
Montserrat Calderón-Santoyo ◽  
Juan Arturo Ragazzo-Sánchez ◽  
Peter Knauth
Keyword(s):  
Biological Control ◽  
Biological Control Agents ◽  
Tropical Fruits
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