scholarly journals Yeast biomass production with potential for biological control: process strategies for increasing yield

2020 ◽  
Vol 9 (4) ◽  
pp. e169943057
Author(s):  
Ana Paula Colares de Andrade ◽  
Helder Levi da Silva ◽  
Gustavo Adolfo Saavedra Pinto
Keyword(s):  
Biological Control ◽  
Carbon Source ◽  
Biomass Production ◽  
Batch Process ◽  
Aeration Rate ◽  
Total Biomass ◽  
Fed Batch ◽  
Promising Alternative ◽  
Yeast Biomass ◽  
Feed Medium

The quality of vegetable products is directly linked to the techniques used in the field, in order to ensure safe and healthy products to health. In this context, the use of yeasts with potential for biological control proved to be a promising alternative to assure the safety of these foods. Fermentation processes have been used to promote the development of many products, including the production of yeast biomass. The objective of this work was to verify the influence of the aeration rate and the fed batch process in the production of yeast biomass. A yeast strain with biological control potential, belonging to Embrapa's Semi-Arid crop collection, was subjected to simple batch cultivation and fed with different aeration rates (3, 4, 6 and 8 L.ar/min) and concentration of carbon source in the feed medium (200, 400 and 600 g/L). The highest biomass (6.99 g/L) after 24 hours of fermentation was observed in the experiment that used an aeration rate of 8 L.ar/min. Regarding the concentration of the carbon source in the feed medium, it was found that the concentration of 200 g/L favored a greater total biomass (11.21 g/L) and reduced the production of ethanol (0.65 g/L ), while the concentration of 600 g/L favored less biomass production (7.90 g/L) and higher ethanol production (9.26 g/L). Thus, it was found that the aeration rate and the fed batch process favor the fermentation strategy, as they contribute to the production of yeast biomass and the overall yield of the process.

scholarly journals Influence of carbon source, agitation and aeration rates for production yeast biomass which potential of use for biological control

2020 ◽  
Vol 9 (4) ◽  
pp. e174943066
Author(s):  
Ana Paula Colares de Andrade ◽  
Helder Levi da Silva ◽  
Gustavo Adolfo Saavedra Pinto
Keyword(s):  
Biological Control ◽  
Carbon Source ◽  
Invert Sugar ◽  
Aeration Rate ◽  
Tropical Fruits ◽  
Bioreactor Cultivation ◽  
Yeast Biomass ◽  
Natural Microflora ◽  
Orbital Shaker ◽  
Agitation And Aeration

The fermentation processes are used to promote better results when it is desired to increase the biomass of organisms that can be used in biological control. Thus, the aim of this work was to expand the scale of production of biomass of yeasts that have potential for biological control. A total of five strains of yeasts isolated from natural microflora of tropical fruits, a strain was selected and evaluated for the influence of the carbon source, cultivation orbital shaker and bioreactor. The tests performed demonstrate that the use of invert sugar as carbon source favored production of biomass material when compared to that obtained with glucose. The bioreactor cultivation of the yeast strain has enhanced performance, since it favors an increase of 24.12% (6.47 to 8.03 g.L-1) in biomass production when compared to the fermentation orbital shaker and that may be associated with increased agitation and aeration rate.

Production of Rhamnolipid Biosurfactant from Fed Batch Culture by Pseudomonas aeruginosa using Multiple Substrates

2020 ◽  
Vol 16 (6) ◽  
pp. 928-933
Author(s):  
Jujjavarapu S. Eswari
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Batch Process ◽  
Time Interval ◽  
Fed Batch ◽  
Multiple Substrates ◽  
Surface Active Agents ◽  
Limiting Nutrients ◽  
Limiting Nutrient ◽  
Surface Active ◽  
Rhamnolipid Biosurfactant

Objective: Biosurfactants are the surface active agents which are used for the reduction of surface and interfacial tensions of liquids. Rhamnolipids are the surfactants produced by Pseudomonas aeruginosa. It requires minimum nutrition for its growth as it can also grow in distilled water. The rhamnolipids produced by Pseudomonas aeruginosa are extra-cellular glycolipids consisting of L-rhamnose and 3-hydroxyalkanoic acid. Methods: The fed-batch method for the rhamnolipid production is considered in this study to know the influence of the carbon, nitrogen, phosphorous substrates as growth-limiting nutrients. Pulse feeding is employed for limiting nutrient addition at particular time interval to obtain maximum rhamnolipid formation from Pseudomonas aeruginosa compared with the batch process. Results: Out of 3 fed batch strategies constant glucose fed batch strategy shows best and gave maximum rhamnolipid concentration of 0.134 g/l.

Contrasting yield formation characteristics in two super-rice hybrids that differ in growth duration

2021 ◽  
pp. 1-10
Author(s):  
Min Huang ◽  
Zui Tao ◽  
Tao Lei ◽  
Fangbo Cao ◽  
Jiana Chen ◽  
...  
Keyword(s):  
Solar Radiation ◽  
Biomass Production ◽  
Field Experiments ◽  
Crop Improvement ◽  
Total Biomass ◽  
Growth Duration ◽  
Area Index ◽  
Rice Hybrid ◽  
Use Efficiency ◽  
Yield Formation

Summary The development of high-yielding, short-duration super-rice hybrids is important for ensuring food security in China where multiple cropping is widely practiced and large-scale farming has gradually emerged. In this study, field experiments were conducted over 3 years to identify the yield formation characteristics in the shorter-duration (∼120 days) super-rice hybrid ‘Guiliangyou 2’ (G2) by comparing it with the longer-duration (∼130 days) super-rice hybrid ‘Y-liangyou 1’ (Y1). The results showed that G2 had a shorter pre-heading growth duration and consequently a shorter total growth duration compared to Y1. Compared to Y1, G2 had lower total biomass production that resulted from lower daily solar radiation, apparent radiation use efficiency (RUE), crop growth rate (CGR), and biomass production during the pre-heading period, but the grain yield was not significantly lower than that of Y1 because it was compensated for by the higher harvest index that resulted from slower leaf senescence (i.e., slower decline in leaf area index during the post-heading period) and higher RUE, CGR, and biomass production during the post-heading period. Our findings suggest that it is feasible to reduce the dependence of yield formation on growth duration to a certain extent in rice by increasing the use efficiency of solar radiation through crop improvement and also highlight the need for a greater fundamental understanding of the physiological processes involved in the higher use efficiency of solar radiation in super-rice hybrids.

scholarly journals Production of High Purity Biosurfactants Using Heavy Oil Residues as Carbon Source

Energies ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3557
Author(s):  
Athina Mandalenaki ◽  
Nicolas Kalogerakis ◽  
Eleftheria Antoniou
Keyword(s):  
Carbon Source ◽  
Heavy Oil ◽  
Oil Pollution ◽  
Batch Reactor ◽  
Production Method ◽  
Promising Alternative ◽  
Heavy Oil Residues ◽  
Oil Residue ◽  
Increasing Demand ◽  
Heavy Oil Residue

Typically, oil pollution cleanup procedures following first response actions include dispersion. Crude oil is biodegradable, and its bioavailability can be increased when dispersed into very fine droplets by means of chemical surfactants. Although their use is widely spread in many applications, the latter may prove toxic, depending on the extent of use. The use of biological means, such as bioremediation and biosurfactants, has emerged over the past years as a very promising ‘green’ alternative technology. Biosurfactants (BSs) are amphiphilic molecules produced by microorganisms during biodegradation, thus increasing the bioavailability of the organic pollutants. It is their biodegradability and low toxicity that render BSs as a very promising alternative to the synthetic ones. Alcanivorax borkumensis SK2 strain ability to produce BSs, without any impurities from the substrate, was investigated. The biosurfactant production was scaled up by means of a sequencing batch reactor (SBR) and a heavy oil residue substrate as the carbon source. The product is free from substrate impurities, and its efficiency is tested on oil bioremediation in the marine environment. The product’s dispersion efficiency was determined by the baffled flask test. The production method proposed can have a significant impact to the market, given the ever-increasing demand for ecologically friendly, reliable, commercially viable and economically competitive environmental cleanup techniques.

scholarly journals Establishing a Fed‐Batch Process for Protease Expression with Bacillus licheniformis in Polymer‐Based Controlled‐Release Microtiter Plates

2019 ◽  
Vol 15 (2) ◽  
pp. 1900088 ◽  
Author(s):  
Tobias Habicher ◽  
Edward K. A. Rauls ◽  
Franziska Egidi ◽  
Timm Keil ◽  
Tobias Klein ◽  
...  
Keyword(s):  
Controlled Release ◽  
Bacillus Licheniformis ◽  
Batch Process ◽  
Fed Batch ◽  
Microtiter Plates ◽  
Protease Expression

Role of sink-source relationships in chrysanthemum flower size and total biomass production

2006 ◽  
Vol 128 (2) ◽  
pp. 263-273 ◽  
Author(s):  
Susana M.P. Carvalho ◽  
Ep Heuvelink ◽  
Jeremy Harbinson ◽  
Olaf Van Kooten
Keyword(s):  
Biomass Production ◽  
Flower Size ◽  
Total Biomass

scholarly journals Scale-up of dextransucrase production by Leuconostoc mesenteroides in fed batch fermentation

2003 ◽  
Vol 46 (3) ◽  
pp. 455-459 ◽  
Author(s):  
Georgina L. Michelena ◽  
Aidín Martínez ◽  
Antonio Bell ◽  
Emilia Carrera ◽  
Roxana Valencia
Keyword(s):  
Oxygen Transfer ◽  
Enzyme Production ◽  
Batch Fermentation ◽  
Leuconostoc Mesenteroides ◽  
Scale Up ◽  
Oxygen Transfer Rate ◽  
Maximum Growth ◽  
Aeration Rate ◽  
Fed Batch ◽  
Fed Batch Fermentation

Fed batch fermentation was carried out for the dextransucrase enzyme production from Leuconostoc mesenteroides and the production was scale-up using oxygen transfer criteriuom. It was found that in 5 L vessel fermentation capacity, the best agitation speed was 225 min-1 and aeration rate was 0.15 vvm, obtaining dextransucrase activity of 127 DSU/mL.. The maximum enzyme production velocity coincide with the maximum growth velocity between 6 and 7 h of fermentation, which confirmed that dextransucrase production was associated with microbial growth. High enzyme yields were achieved during scale up based on oxygen transfer rate.

scholarly journals Xylitol production by Candida parapsilosis under fed-batch culture

2001 ◽  
Vol 44 (2) ◽  
pp. 125-128 ◽  
Author(s):  
Sandra A. Furlan ◽  
Heizir F. de Castro
Keyword(s):  
Candida Parapsilosis ◽  
Batch Cultivation ◽  
Xylitol Production ◽  
Fed Batch ◽  
Substrate Consumption ◽  
Feed Medium ◽  
Fed Batch Culture ◽  
Rates Of Growth ◽  
Fed Batch Cultivation ◽  
Single Batch

Xylitol production by Candida parapsilosis was investigated under fed-batch cultivation, using single (xylose) or mixed (xylose and glucose) sugars as substrates. The presence of glucose in the medium induced the production of ethanol as secondary metabolite and improved specific rates of growth, xylitol formation and substrate consumption. Fractionated supply of the feed medium at constant sugar concentration did not promote any increase on the productivity compared to the single batch cultivation.

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