scholarly journals Supplemented Creatine Induces Changes in Human Metabolism of Thiocompounds and One- and Two-Carbon Units

2010 ◽  
pp. 431-442
Author(s):  
T Navrátil ◽  
E Kohlíková ◽  
M Petr ◽  
D Pelclová ◽  
M Heyrovský ◽  
...  
Keyword(s):  
Urinary Excretion ◽  
Vitamin B12 ◽  
Metabolic Pathways ◽  
Blood Levels ◽  
Redox Processes ◽  
Human Metabolism ◽  
Metabolic Disturbances ◽  
Creatinine Excretion ◽  
Thiodiglycolic Acid ◽  
The Last Man

The administration of creatine (5 g/day for one month) to 11 young active sportsmen affected their urinary excretion of creatine, creatinine, and thiodiglycolic acid (TDGA) as well as blood levels of homocysteine, vitamin B12 and folates. The probands were divided into four groups, according to the amount of creatine found in urine, and of folates and vitamin B12 determined in blood. The changes of folates and vitamin B12 were mutually reciprocal. Each group utilized CR as donor of one- and two-carbon (1C and 2C) units by means of homocysteine (HoCySH), folates, and vitamin B12, in different metabolic pathways. In 10 men the creatine administration was accompanied by an increase of HoCySH level in blood, while in the last man, with accidentally discovered hyperhomocysteinemia, the HoCySH level dropped by 50 %. Differences between initial and terminal TDGA levels indicate that creatine affects equilibria of redox processes. Creatinine excretion into urine changed in the dependence on the extent of metabolic disturbances.

scholarly journals Hydroxocobalamin. I. Blood Levels and Urinary Excretion of Vitamin B12 in Man After a Single Parenteral Dose of Aqueous Hydroxocobalamin, Aqueous Cyanocobalamin and Cyanocobalamin Zinc-Tannate Complex

Blood ◽  
1961 ◽  
Vol 18 (5) ◽  
pp. 511-521 ◽  
Author(s):  
GEORGE B. JERZY GLASS ◽  
HELEN R. SKEGGS ◽  
DUK HO LEE ◽  
E. LINN JONES ◽  
WILLIAM W. HARDY
Keyword(s):  
Urinary Excretion ◽  
Vitamin B12 ◽  
Blood Level ◽  
Intramuscular Injection ◽  
Serum Vitamin ◽  
Blood Levels ◽  
Parenteral Dose ◽  
Single Intramuscular Injection

Abstract A single intramuscular injection of 500 or 1000 µg. of hydroxocobalamin to 17 individuals resulted in a 1.8- to 4.1-times higher mean serum vitamin B12 blood level, respectively, 5 hours after injection; a 4.6- and 12.8-times higher level 24 hours after injection; a 2.4- and 5.2-times higher level 72 hours after injection, and a 1.6- and 2.4-times higher level by the 2nd through the 4th week after injection than identical doses of cyanocobalamin administered to 19 individuals. The vitamin B12 blood levels following i.m. administration of 500 or 1000 µg. of hydroxocobalamin were significantly higher during the first 24 and 48 hours, respectively, than they were after a cyanocobalamin zinc-tannate complex given to 17 individuals at identical doses. After a single i.m. injection of 500 or 1000 µg. of hydroxocobalamin, an average of only 16 per cent and 27 per cent, respectively, of the vitamin B12 was lost in the 72-hour urines, as compared to 60 per cent and 69 per cent, respectively, after identical doses of cyanocobalamin. These differences, again, were highly significant statistically. The results of these studies give evidence of a slower rate of urinary excretion of hydroxocobalamin as compared to that of cyanocobalamin, and of its ability to build up consistently higher and more prolonged vitamin B12 levels in the blood.

Human metabolism and urinary excretion kinetics of di-n-butyl adipate (DnBA) after oral and dermal administration in three volunteers

2021 ◽  
Vol 343 ◽  
pp. 11-20
Author(s):  
Benedikt Ringbeck ◽  
Daniel Bury ◽  
Alexandra Gotthardt ◽  
Heiko Hayen ◽  
Rainer Otter ◽  
...  
Keyword(s):  
Urinary Excretion ◽  
Human Metabolism ◽  
Excretion Kinetics ◽  
Dermal Administration ◽  
Kinetics Of

scholarly journals Ultra-Performance Liquid Chromatography/Mass Spectrometry-Based Metabolomics for Discovering Potential Biomarkers and Metabolic Pathways of Colorectal Cancer in Mouse Model (ApcMin/+) and Revealing the Effect of Honokiol

2021 ◽  
Vol 11 ◽  
Author(s):  
Xin Chen ◽  
Bo-lun Shi ◽  
Run-zhi Qi ◽  
Xing Chang ◽  
Hong-gang Zheng
Keyword(s):  
Colorectal Cancer ◽  
Mouse Model ◽  
Metabolic Pathways ◽  
Molecular Mechanisms ◽  
Tca Cycle ◽  
Ultra Performance Liquid Chromatography ◽  
Therapeutic Effects ◽  
Anticancer Activities ◽  
Metabolic Disturbances ◽  
Endogenous Metabolites

Endogenous metabolites are a class of molecules playing diverse and significant roles in many metabolic pathways for disease. Honokiol (HNK), an active poly-phenolic compound, has shown potent anticancer activities. However, the detailed crucial mechanism regulated by HNK in colorectal cancer remains unclear. In the present study, we investigated the therapeutic effects and the underlying molecular mechanisms of HNK on colorectal cancer in a mouse model (ApcMin/+) by analyzing the urine metabolic profile based on metabolomics, which is a powerful tool for characterizing metabolic disturbances. We found that potential urine biomarkers were involved in the metabolism of compounds such as purines, tyrosines, tryptophans, etc. Moreover, we showed that a total of 27 metabolites were the most contribution biomarkers for intestinal tumors, and we found that the citrate cycle (TCA cycle) was regulated by HNK. In addition, it was suggested that the efficacy of HNK was achieved by affecting the multi-pathway system via influencing relevant metabolic pathways and regulating metabolic function. Our work also showed that high-throughput metabolomics can characterize the regulation of metabolic disorders as a therapeutic strategy to prevent colorectal cancer.

Analiza obrazu klinicznego niedokrwistości z niedoboru witaminy B12 u dzieci

2019 ◽  
Vol 23 (1) ◽  
Author(s):  
Aleksandra Pankiewicz ◽  
Anna Adamowicz-Salach ◽  
Marek W. Karwacki ◽  
Katarzyna Pawelec ◽  
Katarzyna Albrecht ◽  
...  
Keyword(s):  
Vitamin B12 ◽  
Metabolic Pathways ◽  
Essential Role ◽  
Vitamin B12 Deficiency ◽  
Neurological Damage ◽  
Laboratory Findings ◽  
Delay In Diagnosis ◽  
B12 Deficiency ◽  
Late Manifestation

Diagnosis of vitamin B12 deficiency could be difficult due to various and rather unspecific symptoms and often late manifestation in hematological findings. B12 has a crucial role as cofactor of many metabolic pathways and has essential role in many processes such as DNA synthesis, neuron myelination and gluconeogenesis. Blood smear has a huge role in early diagnosis and should be performed. B12 deficiency is not very common, however could be underestimated especially in developing countries. In children main cause of deficiency is exclusively breastfeeding by vitamin B12 depleted mothers. Consequences of low intake in children are more severe than in adults, because of lower liver storage. Early detection and treatment is very important, because long term deficiency could result in persistent neurological damage. Coexistence iron or folate deficiencies could result delay in diagnosis. In this article different manifestation and laboratory findings in group of children with B12 deficiency is described.

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