scholarly journals The Interaction of Osteoblasts With Bone-Implant Materials: 1. The Effect of Physicochemical Surface Properties of Implant Materials

2011 ◽  
pp. 95-111 ◽  
Author(s):  
D. KUBIES ◽  
L. HIMMLOVÁ ◽  
T. RIEDEL ◽  
E. CHÁNOVÁ ◽  
K. BALÍK ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Free Energy ◽  
Blood Coagulation ◽  
Surface Free Energy ◽  
Blood Clot ◽  
Surface Treatments ◽  
Clot Formation ◽  
Osteoblast Proliferation ◽  
Human Osteoblasts ◽  
Implant Materials

This comparative study of various surface treatments of commercially available implant materials is intended as guidance for orientation among particular surface treatment methods in term of the cell reaction of normal human osteoblasts and blood coagulation. The influence of physicochemical surface parameters such as roughness, surface free energy and wettability on the response of human osteoblasts in the immediate vicinity of implants and on the blood coagulation was studied. The osteoblast proliferation was monitored and the expression of tissue mediators (TNF-α, IL-8, MMP-1, bone alkaline phosphatase, VCAM-1, TGF-β) was evaluated after the cell cultivation onto a wide range of commercially available materials (titanium and Ti6Al4V alloy with various surface treatments, CrCoMo alloy, zirconium oxide ceramics, polyethylene and carbon/carbon composite). The formation of a blood clot was investigated on the samples immersed in a freshly drawn whole rabbit blood using scanning electron microscope. The surfaces with an increased osteoblast proliferation exhibited particularly higher surface roughness (here Ra > 3.5 µm) followed by a high polar part of the surface free energy whereas the effect of wettability played a minor role. The surface roughness was also the main factor regulating the blood coagulation. The blood clot formation analysis showed a rapid coagulum formation on the rough titanium-based surfaces. The titanium with an etching treatment was considered as the most suitable candidate for healing into the bone tissue due to high osteoblast proliferation, the highest production of osteogenesis markers and low production of inflammatory cytokines and due to the most intensive blood clot formation.

Analysis of surface roughness and surface free energy characteristics of various orthodontic materials

2009 ◽  
Vol 136 (5) ◽  
pp. 668-674 ◽  
Author(s):  
Hyo-Beom Ahn ◽  
Sug-Joon Ahn ◽  
Shin-Jae Lee ◽  
Tae-Woo Kim ◽  
Dong-Seok Nahm
Keyword(s):  
Surface Roughness ◽  
Free Energy ◽  
Surface Free Energy ◽  
Energy Characteristics ◽  
Orthodontic Materials

The influence of surface free energy and surface roughness on early plaque formation. An in vivo study in man

1990 ◽  
Vol 17 (3) ◽  
pp. 138-144 ◽  
Author(s):  
M. Quirynen ◽  
M. Marechal ◽  
H. J. Busscher ◽  
A. H. Weerkamp ◽  
P. L. Darius ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Free Energy ◽  
Surface Free Energy ◽  
Plaque Formation ◽  
In Vivo Study

Hemostasis and its Regulation

2016 ◽  
Author(s):  
Lawrence L K Leung
Keyword(s):  
Endothelial Cells ◽  
Blood Coagulation ◽  
Protein C ◽  
Formation Control ◽  
Blood Clot ◽  
Protein S ◽  
Clot Formation ◽  
Tissue Type ◽  
Control Mechanisms ◽  
Clotting Cascade

Hemostasis, the process of blood clot formation, is a coordinated series of responses to vessel injury. It requires complex interactions between platelets, the clotting cascade, blood flow and shear, endothelial cells, and fibrinolysis. This review covers platelet plug formation, clotting cascade, initiation and propagation of blood clot formation, control mechanisms, overview of blood coagulation, blood coagulation as part of the host defense system, heterogeneity of endothelial cells and vascular bed–specific hemostasis, platelet production and thrombopoietin, and coagulation tests and their use. Figures show activated platelets, platelet aggregation, the classic and revised view of the clotting cascade, the inhibition of thrombin by antithrombin, the protein C/protein S pathway, the synergism between nitric oxide (NO) and prostacyclin (PGI2), tissue-type plasminogen activator, the transformation of fibrinogen to fibrin, activated protein C (APC) and carboxypeptidase B-2 (CPB-2) at the site of vascular injury, and an algorithm detailing the exposure of tissue factor at a vascular wound that initiates the clotting cascade. The table lists natural antithrombotic mechanisms of endothelial cells. This review contains 10 highly rendered figures, 1 table, and 45 references.

The influence of surface free energy and surface roughness on early plaque formation.

1992 ◽  
Vol 17 (3) ◽  
pp. 138-144 ◽  
Author(s):  
M. Quirynen ◽  
M. Marechal ◽  
H. J. Busscher ◽  
A. H. Weerkamp ◽  
P. L. Darius ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Free Energy ◽  
Surface Free Energy ◽  
Plaque Formation

The bacterial colonization of intra‐oral hard surfacesin vivo:Influence of surface free energy and surface roughness

Biofouling ◽  
1991 ◽  
Vol 4 (1-3) ◽  
pp. 187-198 ◽  
Author(s):  
M Quirynen ◽  
M Marechal ◽  
D Van Steenberghe ◽  
H J Busscher ◽  
H C Van Der Mei
Keyword(s):  
Surface Roughness ◽  
Free Energy ◽  
Surface Free Energy ◽  
Bacterial Colonization

scholarly journals Citric Acid Passivation of Titanium Dental Implants for Minimizing Bacterial Colonization Impact

Coatings ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 214
Author(s):  
Miquel Punset ◽  
Javi Vilarrasa ◽  
José Nart ◽  
José María Manero ◽  
Begoña Bosch ◽  
...  
Keyword(s):  
Contact Angle ◽  
Free Energy ◽  
Citric Acid ◽  
Dental Implants ◽  
Surface Free Energy ◽  
Bacterial Colonization ◽  
Surface Treatments ◽  
Average Roughness ◽  
Physical Chemical ◽  
Static Contact

Surface topography and physical-chemical properties usually play a key-role in both osseointegration improvement and bacterial colonization reduction over the surface of dental implants. The aim of this study is to compare the chemical and bacteriological behavior of two different acid passivation surface treatments on titanium c.p. grade 3 used for dental implant manufacturing. Surface roughness was evaluated using White Light Interferometry (WLI) in order to determine different roughness parameters such as average roughness (Sa), the spacing parameter (Sm) and the hybrid parameter of surface index area (SIA). Contact angle (CA) and surface free energy (SFE) were evaluated in order to establish the surface wettability of the different groups of samples. Titanium ion-release from the different samples was also been analyzed in Hank’s solution medium at 37 °C by using Inductively Coupled Plasma Mass Spectrometry (ICP-MS) at different immersion times. Bacterial viability adhesion assays were done using S. sanguinis (CECT 480, Spain) as a bacterial strain model of primary colonizer in oral biofilm formation. The bacteria attachment and morphology on Ti surfaces were determined using a live/dead staining method after 4 h of incubation and further analyzed by scanning electron microscope (SEM). Acid passivation surface treatments produced a statistically-significant (p < 0.05) roughness increase in all the evaluated parameters (Sa, Sm, SAI). The treatment with citric acid decreased the static contact angle (CA) and caused an increase in surface free energy (SFE) with a high polarization and oxidizing character. These physical-chemical surface characteristics obtained by means of citric acid passivation caused the bactericidal behavior as it has been proved in bacterial studies.

scholarly journals Measurements of surface free energy as a tool to asses the effect of varnishing and printing of the paper substrates

2020 ◽  
Author(s):  
Zuzanna Żołek-Tryznowska ◽  
◽  
Marta Więcek ◽  
Keyword(s):  
Surface Roughness ◽  
Contact Angle ◽  
Free Energy ◽  
Surface Free Energy ◽  
Water Contact Angle ◽  
Water Contact ◽  
Human Eye ◽  
Varnish Layer ◽  
Energy Determination ◽  
Paper Substrates

Nowadays, printing products might be finished in various ways. Varnishing process is one of the most popular finishing method which gives various effect, such as mate, glossy etc. However, the varnish layer applied on the paper is very thin, therefore it can be invisible to the naked human eye. The aim of this work was to use contact angle measurement and surface free energy determination as a tool to assess the effect of printing and varnishing process of paper materials. We have used various tools in order to analyses the changes of surface: surface roughness, gloss, water contact angle absorption and surface free energy determination. Those tools were used in order to confirm whether the print has been covered with varnish or not. In this work six various paper substrates were used (glossy, coated and un-coated papers). The printing and varnishing was performed in laboratory conditions using flexographic water-based printing and waterbased varnish. Samples were prepared as follows: paper with ink coating, paper with varnish coating and paper with ink layer and varnish layer on the top. The surface roughness was determinated of all samples and compared. The surface roughness changes were observed for pure paper, overprinted and overvarnished. Next, the gloss of samples prior and after printing and varnishing was measured. The gloss of the samples increases when they are printed or varnished, what is related with properties of ink and varnish. Also, the thickness of ink and varnish layers was determinated. The thickness of the samples increases when the number of layers increases. Finally, the water contact angle was measured and surface free energy was calculated with Owens-Wendt method. Our results reveal the possibility of using various tools in order to confirm the performance of varnishing of the prints. The printing with various colors is always seen by the naked human eye. On the other hand, the varnish layer might be not visible. However, such a comparison is not possible if we do not have the pure paper substrates prior printing or varnish.

scholarly journals Influence of the Manufacturing Method on the Adhesion of Candida albicans and Streptococcus mutans to Oral Splint Resins

Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1534
Author(s):  
Andrea Schubert ◽  
Ralf Bürgers ◽  
Franziska Baum ◽  
Oliver Kurbad ◽  
Torsten Wassmann
Keyword(s):  
Surface Roughness ◽  
Free Energy ◽  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Surface Free Energy ◽  
Computer Aided Design ◽  
Pearson Correlation ◽  
Oral Diseases ◽  
Microbial Adhesion ◽  
Computer Aided

Microbial adhesion to oral splints may lead to oral diseases such as candidiasis, periodontitis or caries. The present in vitro study aimed to assess the effect of novel computer-aided design/computer-aided manufacturing (CAD/CAM) and conventional manufacturing on Candida albicans and Streptococcus mutans adhesion to oral splint resins. Standardized specimens of four 3D-printed, two milled, one thermoformed and one pressed splint resin were assessed for surface roughness by widefield confocal microscopy and for surface free energy by contact angle measurements. Specimens were incubated with C. albicans or S. mutans for two hours; a luminometric ATP assay was performed for the quantification of fungal and bacterial adhesion. Both one-way ANOVA with Tukey post hoc testing and Pearson correlation analysis were performed (p < 0.05) in order to relate manufacturing methods, surface roughness and surface free energy to microbial adhesion. Three-dimensional printing and milling were associated with increased adhesion of C. albicans compared to conventional thermoforming and pressing, while the S. mutans adhesion was not affected. Surface roughness and surface free energy showed no significant correlation with microbial adhesion. Increased fungal adhesion to oral splints manufactured by 3D printing or milling may be relevant for medically compromised patients with an enhanced risk for developing candidiasis.

scholarly journals Evaluation of surface characteristics of Ti-6Al-4V and Tilite alloys used for implant abutments

2006 ◽  
Vol 20 (4) ◽  
pp. 307-311 ◽  
Author(s):  
Emilena Maria Castor Xisto Lima ◽  
Wander José da Silva ◽  
Juliana Silva Moura ◽  
Fernanda Faot ◽  
Altair Antoninha Del Bel Cury
Keyword(s):  
Surface Roughness ◽  
Free Energy ◽  
Surface Morphology ◽  
Surface Free Energy ◽  
Surface Hardness ◽  
Indentation Test ◽  
Surface Characteristics ◽  
Biological Responses ◽  
Implant Abutments ◽  
Micro Indentation

The aim of this study was to evaluate surface free energy (SFE), surface roughness (SR) and surface hardness (SH) of two commercially available materials for fabricating dental implant abutments. In addition, the specimens were investigated by scanning electron microscopy (SEM) to determine the surface morphology. Twenty five discs (5 x 2 mm) of Ti-6Al-4V and Tilite (Ni-Cr-Ti) alloys were used in this study. Surface free energy was determined by the contact angle formed between a drop of distilled, deionized water and the surface of the specimen of each material. The surface roughness was measured with a mechanical profilometer and the surface hardness was evaluated by means of the Vickers hardness micro indentation test. SFE, SR and SH data were analyzed using one-way ANOVA (p < 0.05). Statistical differences (p < 0.05) were found between Ti-6Al-4V (36.2 erg.cm-2; 0.2 µm) and Tilite (30.9 erg.cm-2; 0.16 µm) for SFE and SR. However, the differences between the surface hardness values of Ti-6Al-4V (325.0 kg/mm²) and Tilite (324.3 kg/mm²) were not statistically significant (p > 0.05). Evaluations by SEM revealed different surface morphology. Within the limits of this study, it can be concluded that the Ti-6Al-4V and Tilite alloys showed differences in surface properties, except for surface hardness, suggesting that both alloys may be considered appropriate for producing abutments. Further studies are, however, necessary to elucidate the biological responses to implant abutments made with these alloys.

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