scholarly journals Receptor-mediated endocytosis is an important way for gelatin nano-particles penetration into cells

2020 ◽  
pp. 3-10
Author(s):  
Alla I. Potapovich ◽  
Tatyana O. Suhan ◽  
Tatsiana G. Shutava ◽  
Vladimir A. Kostyuk
Keyword(s):  
Cancer Cells ◽  
Human Cancer ◽  
Human Fibroblasts ◽  
Nano Particles ◽  
Gelatin Nanoparticles ◽  
Human Cancer Cells ◽  
Collagen Receptors ◽  
Normal Human ◽  
Pharmacologically Active ◽  
Active Substances

Experimental data detailing the possibilities of using gelatin nanoparticles obtained by the two-stage desolvation method without the use of surfactants for delivering pharmacologically active substances to cultured normal and human cancer cells are presented. It was shown that clathrin-dependent endocytosis is the main route of entry of such nanoparticles into normal human fibroblasts and cancer cells of the MDA-MB-231 line. Due to this type of endocytosis, more than 50 % of gelatin nanoparticles enter the cells. It was shown that in the process of clathrin-dependent endocytosis, gelatin nanoparticles specifically bind to collagen receptors.

Anti-neoplastic agent thymoquinone induces degradation of α and β tubulin proteins in human cancer cells without affecting their level in normal human fibroblasts

2011 ◽  
Vol 30 (5) ◽  
pp. 1813-1819 ◽  
Author(s):  
Mahmoud Alhosin ◽  
Abdulkhaleg Ibrahim ◽  
Abdelaziz Boukhari ◽  
Tanveer Sharif ◽  
Jean-Pierre Gies ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Human Cancer ◽  
Human Fibroblasts ◽  
Human Cancer Cells ◽  
Normal Human ◽  
Β Tubulin

scholarly journals A Novel and Efficient Approach for Screening Cancer Cell Specific Monoclonal Antibodies

2019 ◽  
Author(s):  
Xin-Hui Pei
Keyword(s):  
Monoclonal Antibodies ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Human Liver ◽  
Human Cancer ◽  
Human Cells ◽  
Specific Antibodies ◽  
Human Cancer Cells ◽  
Normal Human ◽  
Normal Human Cells

AbstractCancer cell specific antibodies are pivotal tools in developing new immunotherapies for treating cancers. However, acquirement of cancer cell specific antibodies is time-consuming and often arduous. To circumvent such a barrier, we developed a novel antibody-screening method that can be used to efficiently produce cancer cell specific antibodies by an ‘antibody filter’ mechanism. First, we used normal human cells to perform the immunization in mice and collected the antisera. Second, we used human cancer cells together with the antisera against normal human cells to immunize another batch of mice. Theoretically, the antisera were able to neutralize the antigens from normal human cells, and therefore specific antigens only expressed in cancer cells could take advantage of the immunization. Third, we screened positive clones that are specific for cancer cells but not normal cells. Using this conceptual method, we successfully obtained 11 monoclonal antibodies that are specific for a human liver cancer cells line (HepG2) but not for a normal human liver cell line (HH). In addition, these clones failed to recognize other human cancer cells originated from different tissues, further highlighting the specificity. Collectively, we provide a novel and effective approach for screening cancer cell specific monoclonal antibodies, which may significantly facilitate the development of new anti-cancer therapeutics.

Isocarbostyril Alkaloids and their Derivatives as Promising Antitumor Agents

2013 ◽  
Vol 411-414 ◽  
pp. 3150-3153
Author(s):  
Yu Bin Ji ◽  
Jia Zheng ◽  
Ning Chen ◽  
Dong Xue Song ◽  
Yan Dong ◽  
...  
Keyword(s):  
Cancer Cells ◽  
High Efficiency ◽  
Antitumor Agents ◽  
Human Cancer ◽  
Hot Spot ◽  
Human Cells ◽  
Human Cancer Cells ◽  
Basic Nitrogen ◽  
Normal Human ◽  
Low Toxicity

socarbostyril alkaloids is a kind of alkaloid does not contain basic nitrogen atoms and is represented by hydroxylated benzophenanthridone or isoquinolinone types of structure. The most widely known compounds of this group are narciclasine, lycoricidine , and pancratistatin. They have demonstrated to inhibite the proliferation of many human cancer cells, and at the same time have no affect on normal human cells under a certain dose, they have a high efficiency and low toxicity in antitumor area. Now this kind of compound has been a hot spot research to antitumor workers. The present paper reviews the origin and the antitumor function of the Isocarbostyril alkaloids.

scholarly journals Hydrogen Peroxide Mediates Artemisinin-Derived C-16 Carba-Dimer-Induced Toxicity of Human Cancer Cells

Antioxidants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 108
Author(s):  
Amanda L. Kalen ◽  
Brett A. Wagner ◽  
Ehab H. Sarsour ◽  
Maneesh G. Kumar ◽  
Jessica L. Reedy ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cancer Cells ◽  
Human Cancer ◽  
Human Fibroblasts ◽  
Parent Compound ◽  
D1 Protein ◽  
Cancer Drug ◽  
Resonance Spectroscopy ◽  
Free System ◽  
Human Cancer Cells

This study used a nitroaliphatic chemistry approach to synthesize a novel artemisinin-derived carba-dimer (AG-1) and determined its anti-proliferative effects in human normal and cancer cells. AG-1 treatments selectively inhibit proliferation of cancer cells compared to normal human fibroblasts. Compared to artemisinin, AG-1 is more toxic to human breast, prostate, head–neck, pancreas and skin cancer cells; 50% inhibition (IC50) 123 µM in AG-1 vs. 290 µM in artemisinin-treated breast cancer cells. AG-1 treatment decreased (~5 folds) cyclin D1 protein expression that correlated with an increase in the percentage of cells in the G1-phase, suggesting a G1 delay. AG-1-induced toxicity was independent of the DNA damage at 72 h post-treatment, as measured by micronuclei frequency and γH2AX protein levels. Results from electron paramagnetic resonance spectroscopy showed Fe-catalyzed formation of AG-1 carbon-centered radicals in a cell-free system. Flow cytometry analysis of H2DCF-DA oxidation showed a significant increase in the steady-state levels of reactive oxygen species (ROS) in AG-1-treated cells. Pre-treatment with N-acetyl-l-cysteine and antioxidant enzymes (superoxide dismutase and catalase) significantly suppressed AG-1-induced toxicity, suggesting that superoxide and hydrogen peroxide contribute to AG-1-induced toxicity in human cancer cells. AG-1 represents a novel class of anti-cancer drug that is more potent than its parent compound, artemisinin.

Aqueous Extract of Phyllanthus urinaria Induces Apoptosis in Human Cancer Cells

2004 ◽  
Vol 32 (02) ◽  
pp. 175-183 ◽  
Author(s):  
Sheng-Teng Huang ◽  
Rong-Chi Yang ◽  
Jong-Hwei S. Pang
Keyword(s):  
Cancer Cells ◽  
Aqueous Extract ◽  
Vascular Endothelial Cells ◽  
Human Cancer ◽  
Morphological Changes ◽  
Toxic Side Effect ◽  
Human Cancer Cells ◽  
Phyllanthus Urinaria ◽  
Normal Human ◽  
Different Origins

Cell apoptosis is now known to play an important role in the maintenance of cellular homeostasis and anti-carcinogenesis. The anticancer effect of aqueous extract prepared from Phyllanthus urinaria (P. urinaria) was investigated by analyzing its potential to induce apoptosis in human cancer cells. We showed that the aqueous extract of P. urinaria could reduce the viability by inducing the apoptosis in human cancer cells derived from several different origins as demonstrated by morphological changes and DNA fragmentation. Yet, P. urinaria extract exhibited no cytotoxic effect on normal human cells, including vascular endothelial cells and liver cells under the same conditions. It suggests that the aqueous extract of P. urinaria is substantially useful in treating various kinds of human cancer cells without toxic side effect on normal cells.

Indirubin derivatives inhibit SFKs/Stat signaling associated with apoptosis in human cancer cells

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
S Nam ◽  
R Buettner ◽  
X Liu ◽  
J Turkson ◽  
D Kim ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Human Cancer ◽  
Human Cancer Cells ◽  
Stat Signaling
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