Effects of a Single and Course Application of Remote Ischemic Preconditioning on the Physical Performance of Laboratory Animals

Previous publications reported the use of remote ischemic preconditioning (rIPC) as a non-drug method for increasing physical performance. However, the reported data are of a contradictory nature. Aim: to evaluate the potential of rIPC for increasing the physical performance of laboratory animals. The study was performed on 72 white outbred male rats. An assessment of the physical performance was carried out using a modiﬁed Porsolt test. The hind limbs of non-anesthetized animals were subjected to preconditioning. The results of a single and course (during 5 days) application of rIPC was evaluated. A single use of rIPC increased the average swimming time in the experimental group by 38.5%. The course application of rIPC did not lead to signiﬁcant changes in the indicators of physical performance in any of the animal groups.

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An Experimental Model of Liver Cirrhosis in Laboratory Animals

Health and Ecology Issues ◽

10.51523/2708-6011.2019-16-1-14 ◽

2019 ◽

pp. 72-77

Author(s):

D. A. Yevseyenko ◽

Z. A. Dundarov ◽

E. A. Nadyrov

Keyword(s):

Body Weight ◽

Liver Cirrhosis ◽

Olive Oil ◽

The Body ◽

Blood Analysis ◽

Male Rats ◽

Laboratory Animals ◽

Control Group ◽

Experimental Group ◽

Biochemical Blood Analysis

Objective: to develop and justify the application of a new experimental method of the simulation of liver cirrhosis in laboratory animals. Material and methods. The simulation of liver cirrhosis was performed on 11 eugamic white Wistar male rats with the body weight of 203.5 ± 22.2 g (experimental group). The control group consisted of 12 healthy laboratory animals. Acute toxic liver injury resulting in cirrhosis was caused by means of the intraperitoneal administration of 50 % solution of carbon tetrachloride (CCl4) in olive oil on the first day of the experiment at a dosage of 0.1 ml of CCl4 + 0.4 ml of olive oil per 100 g of the body weight of the animals, on the second day of the experiment - 0.3 ml of CCl4 + 0.2 ml of olive oil per 100 g of the body weight of the animals. For synergism and potentiation of the hepatotoxic effect of CCl4, the animals daily had free access to 10% ethanol solution. The duration of the experiment was 65 days. The clinical and laboratory parameters were evaluated, the histological assessment of the preparations was carried out. The obtained data were compared with the same parameters of the control group of the animals. Results. The reproducibility of the model was 81.8% (9 animals). The values of the biochemical blood analysis indicated statistically significant increases in the levels of total bilirubin, serum transaminases (AST, ALT), creatinine, a decrease in the glucose level in the animals of the experimental group. The complex morphological confirmation of liver cirrhosis in progress was obtained. Conclusion. The proposed method of the liver cirrhosis modeling correlate with the values of the biochemical blood analysis, pathological changes in the tissue of the liver and internal organs of liver cirrhosis in humans. With the help of the original model, it is possible to investigate the pathogenesis and effects of various groups of pharmacological drugs on liver cirrhosis and its complications (acute blood loss associated with the syndrome of portal hypertension).

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Influence of Medication-Induced Preconditioning or Remote Ischemic Preconditioning on the Intrinsic Vascular Extracellular RNA/Ribonuclease System in Cardioprotection

The Thoracic and Cardiovascular Surgeon ◽

10.1055/s-0038-1675398 ◽

2018 ◽

Vol 67 (06) ◽

pp. 494-501 ◽

Cited By ~ 1

Author(s):

Konstantin J. Tolkmitt ◽

Sakine Simsekyilmaz ◽

Julia Schipke ◽

Christian Mühlfeld ◽

Klaus T. Preissner ◽

...

Keyword(s):

Ischemic Preconditioning ◽

Structural Parameters ◽

Ischemia Reperfusion ◽

Experimental System ◽

Rnase Activity ◽

Remote Ischemic Preconditioning ◽

Male Rats ◽

Electron Microscopic ◽

Extracellular Rna ◽

Leg Ischemia

Abstract Background It has been demonstrated that remote ischemic preconditioning (RIPC) increases ribonuclease (RNase) levels and protects the heart by reducing extracellular ribonucleic acid (eRNA). As medication-induced preconditioning (MIPC) is also a powerful tool for cardioprotection, we examined the influence of both types of preconditioning on the eRNA/RNase system. Methods In 17 male rats, RIPC (3 × 5 minute hind-leg ischemia) or MIPC (isoflurane and buprenorphine anesthesia) was performed. Five rats served as control and did not undergo preconditioning (non-MIPC). After preconditioning, eRNA levels and RNase activity were determined in plasma, and the hearts were mounted on a blood-perfused Langendorff ischemia/reperfusion apparatus. Hemodynamic, metabolic, and electron microscopic parameters were determined. Furthermore, MIPC with one anesthetic drug only (isoflurane, buprenorphine, or etomidate) was induced in another five rats. After 30 minutes, eRNA levels and RNase activity were determined and compared with an RIPC group (n = 5). Results The plasma of RIPC-treated rats had higher RNase activity and lower eRNA levels than that of MIPC-treated rats. In addition, RIPC increased RNase activity more than MIPC with one drug alone. The RNase activity and eRNA levels in these MIPC groups differed considerably. Hemodynamic parameters of RIPC- and MIPC-treated hearts were better preserved after 90-minute ischemia than those of non-MIPC hearts. No obvious differences were noted between MIPC and RIPC regarding hemodynamics, metabolism, or structural parameters. Conclusions Our results suggest that RIPC does not have any additional cardioprotective benefit in this experimental system. However, the influence of RIPC on the eRNA/RNase system was greater than that of MIPC.

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Modification of a model of non-alcoholic fat liver disease in rats with a сombination of a hypercaloric diet and hypodynamia

Drug development & registration ◽

10.33380/2305-2066-2021-10-4(1)-155-165 ◽

2021 ◽

Vol 10 (4) ◽

pp. 155-165

Author(s):

A. V. Bunjat ◽

O. M. Spasenkova ◽

V. E. Karev ◽

A. V. Karavaeva ◽

D. Ju. Ivkin ◽

...

Keyword(s):

Body Weight ◽

Liver Disease ◽

Physical Performance ◽

White Male ◽

Male Rats ◽

Laboratory Animals ◽

Control Group ◽

Alcoholic Fatty Liver ◽

Epididymal Fat ◽

Hypercaloric Diet

Introduction. Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease in the world, and non-alcoholic steatohepatitis is the second most common cause of liver transplantation in the adult population. An urgent task is to find and develop an optimal model of NAFLD in laboratory animals, which would reproduce all the features of this disease in the clinic.Aim. Modification of the NAFLD model in laboratory animals (rats), which allows the obtained data to be transmitted to humans as fully as possible.Materials and methods. The study was conducted on 52 outbred white male rats of the same age. As the basis of the model, a hypercaloric high-fat diet was used with the addition of food appeal enhancers (sodium glutamate and liquid shrimp extract) and for the first-time conditions of hypodynamia were used – restriction of the motor activity of animals using specially designed cells, in which an individual 11 × 18 cm cell was allocated for each individual. The duration of the study was 12 months. In the course of the experiment, body weight, physical performance, biochemical parameters of blood serum and urine in dynamics were assessed, and lethality was recorded. After the end of the study, the mass of internal organs, visceral and epididymal fat was analyzed, and a histological examination of the liver was performed.Results and discussion. In the course of the experimental study, the development of NAFLD in rats of the control group of animals was histologically confirmed. A high mortality rate was revealed in the group of animals with pathology. Compared with animals of the intact group, a statistically significant increase in their body weight, liver weight, visceral and epididymal fat, a decrease in physical performance, disturbances in lipid, carbohydrate and protein metabolism were revealed, as well as signs of deterioration of the protein synthesis and excretory functions of the liver.Conclusion. A number of advantages of the NAFLD model with a combination of a hypercaloric diet and hypodynamic conditions were revealed, including the similarity of the conditions for the formation and pathogenesis of the disease in experimental animals and humans, which ensures the adequacy of data translation from preclinical practice to clinical practice.

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ACUTE SKIN TOXICITY CHARACTERISTICS OF THE DRUG BASED ON MOXIDECTIN

10.31016/978-5-9902341-5-4.2020.21.39-45 ◽

2020 ◽

pp. 39-45

Author(s):

Belykh

Keyword(s):

Body Weight ◽

Single Channel ◽

Skin Toxicity ◽

Male Rats ◽

Laboratory Animals ◽

Control Group ◽

Acute Skin Toxicity ◽

Significant Difference ◽

Experimental Group ◽

Mouse Body Weight

The acute skin toxicity characteristics of the drug for veterinary use “Inspector Mini” were studied at mice and rats. The active ingredient of the drug is moxidectin which belongs to the group of macrocyclic lactones of the milbemycin class. The studies were carried out in the vivarium of VNIIP – FSC VIEV (Moscow, Russia) on 2 experimental and 1 control groups of white outbred male mice of 19–21 g, 10 animals in each group and male rats of 200–230 g, 6 individuals in each. The mass of animals was indicated during application of the drug. The drug was used once without dilution in the form of the provided solution with single-channel mechanical dispensers with a dosing volume of 10–100 μl for mice and 100–1000 μl for rats. The animals in the experimental group 1 were treated at a dose of 10 400 mg/kg (100 μl per 10 g of mouse body weight or 1000 μl per 100 g of rat body weight), animals in the experimental group 2 – at a dose of 5 200 mg/kg (50 μl per 10 g of mouse body weight or 500 μl per 100 g of rat body weight). The animals in control group were not treat with the drug. As a result of the study, it was found that the LD50 of the drug “Inspector Mini” applied to the skin of mice and rats was more than 10 400 mg/kg per animal weight. During clinical examination of laboratory animals from the experimental groups, no signs of intoxication were observed. During the experiment, there was no significant difference (p ≥ 0.05) in the animals weight from the experimental groups within all periods of weighing compared with the control group of analogues.

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