scholarly journals Antibacterial Activity of Coptis chinensis Extract Against Pectobacterium carotovorum subsp. carotovorum

2016 ◽  
Vol 5 (2) ◽  
pp. 61-66
Author(s):  
Stephen Kironji Githeng'u ◽  
Samuel Nyalala ◽  
Liu Gaoqiong
Keyword(s):  
Antibacterial Activity ◽  
Crop Protection ◽  
In Vitro Study ◽  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
Streptomycin Sulphate ◽  
Coptis Chinensis ◽  
Calla Lily ◽  
Carotovorum Subsp

Soft rot of Zantedeschia, caused by Pectobacterium carotovorum subsp. carotovorum (Pcc), has caused a significant worldwide threat to calla lily production. Hence, in order to effectively manage the disease, an intensive management programme aimed at adequte suppression of the pathogen is paramount. In order to investigate the antibacterial effect of a Coptis chinensis extract product against the soft rot- causing bacterium, an in vitro study was set up. Bacterial isolate were obtained from rotting calla lily tubers and maintained in Nutrient Broth under refrigeration. Sterile petri plates containing 15 ml of Nutrient agar were prepared and aseptically inoculated with 0.1 ml an overnight grown culture of a standardized Pectobacterium carotovorum subsp. carotovorum inoculum containing about 1x 108 cfu/ml. The inoculum was spread evenly over the whole surface of the plates. After solidification, 1 ml of the different concentrations of C. chinensis (1, 10, 25, 50 and 100%) were placed individually at the centre of the inoculated petri plates. Positive check was maintained using Streptomycin sulphate (100ppm) and a negative check using sterile distilled water. Eight replicates were maintained for each treatment and the experiment was repeated twice. Results indicated that the lower concentrations of C. chinensis did not cause any inhibition against Pcc. On the other hand, 100% C. chinensis made an inhibition zone comparable to that streptomycin sulphate. Our results demonstrated that C. chinensis has antibacterial activity and therefore feasible for use in crop protection against soft rots caused by Pectobacterium carotovorum subsp. carotovorum.

scholarly journals Effect of some chemicals on incidence of poato soft rot disease in Bangladesh

2017 ◽  
Vol 52 (2) ◽  
pp. 135-140 ◽  
Author(s):  
MM Rahman ◽  
AA Khan ◽  
IH Mian ◽  
AM Akanda ◽  
MZ Alam
Keyword(s):  
Acetic Acid ◽  
Boric Acid ◽  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
Potato Tubers ◽  
Soft Rot Disease ◽  
Bleaching Powder ◽  
Carotovorum Subsp ◽  
Rot Disease

Bactericidal effect was investigated by chemicals against potato soft rot bacteria in vitro and in storage. The chemicals were acetic acid, boric acid, bleaching powder, lactic acid, calcium hydroxide, calcium chloride, potassium chloride and sodium hypo-chloride. Among eight  chemicals only three chemicals viz. acetic acid, boric acid and bleaching powder showed bactericidal activity against potato soft rot bacteria  Pectobacterium carotovorum subsp. carotovorum (E. carotovora subsp. carotovora) P-138 in vitro. Based on the results of in vitro experiment three chemicals, acetic acid, boric acid and bleaching powder were used to control soft rot disease of potato in storage. Fresh potato tubers were dipped in 0.2% solution/suspensions of acetic acid, boric acid and bleaching powder for 30 min. Then soft rot bacteria Pectobacterium carotovorum subsp. carotovorum P-138 was inoculated on potato. Finally potatoes were stored for 22 weeks in net bags in sterilized condition. All the three chemicals significantly decreased the infection rate, loss in weight and increased percentage of disease reduction (PDR) of potato. Boric acid was the most effective in controlling the soft rot disease of potato in storage followed by acetic acid and bleaching powder. So these chemicals may be used for seed purpose storage of potato tubers for year round storage at farmer’s level.Bangladesh J. Sci. Ind. Res. 52(2), 135-140, 2017

scholarly journals Characterization of a New Bacteriocin, Carocin D, from Pectobacterium carotovorum subsp. carotovorum Pcc21

2010 ◽  
Vol 76 (22) ◽  
pp. 7541-7549 ◽  
Author(s):  
Eunjung Roh ◽  
Tae-Ho Park ◽  
Myung-il Kim ◽  
Seungdon Lee ◽  
Sangryeol Ryu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Antibacterial Activity ◽  
Biological Control Agent ◽  
Soft Rot ◽  
Control Agent ◽  
Indicator Strain ◽  
Amino Acid Sequences ◽  
Pectobacterium Carotovorum ◽  
N Terminus

ABSTRACT Two different bacteriocins, carotovoricin and carocin S1, had been found in Pectobacterium carotovorum subsp. carotovorum, which causes soft-rot disease in diverse plants. Previously, we reported that the particular strain Pcc21, producing only one high-molecular-weight bacteriocin, carried a new antibacterial activity against the indicator strain Pcc3. Here, we report that this new antibacterial activity is due to a new bacteriocin produced by strain Pcc21 and named carocin D. Carocin D is encoded by the caroDK gene located in the genomic DNA together with the caroDI gene, which seems to encode an immunity protein. N-terminal amino acid sequences of purified carocin D were determined by Edman degradation. In comparison with the primary translation product of caroDK, it was found that 8 amino acids are missing at the N terminus. This finding proved that carocin D is synthesized as a precursor peptide and that 8 amino acids are removed from its N terminus during maturation. Carocin D has two putative translocation domains; the N-terminal and C-terminal domains are homologous to those of Escherichia coli colicin E3 and Pseudomonas aeruginosa S-type pyocin, respectively. When caroDK and caroDI genes were transformed into carocin D-sensitive bacteria such as Pcc3, the bacteria became resistant to this bacteriocin. Carocin D has one putative DNase domain at the extreme C terminus and showed DNase activity in vitro. This bacteriocin had slight tolerance to heat but not to proteases. The caroDK gene was present in only 5 of 54 strains of P. carotovorum subsp. carotovorum. These results indicate that carocin D is a third bacteriocin found in P. carotovorum subsp. carotovorum, and this bacteriocin can be readily expressed in carocin D-sensitive nonpathogenic bacteria, which may have high potential as a biological control agent in the field.

scholarly journals In vitro efficacy of endophytic bacteria against Pectobacterium carotovorum subsp. carotovorum causing soft rot disease in banana

2021 ◽  
Vol 9 (1) ◽  
pp. 2087-2092
Author(s):  
R Kalaivanan ◽  
K Eraivan Arutkani Aiyanathan ◽  
S Thiruvudainambi ◽  
N Senthil ◽  
A Beaulah ◽  
...  
Keyword(s):  
Endophytic Bacteria ◽  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
Soft Rot Disease ◽  
Carotovorum Subsp ◽  
Rot Disease

scholarly journals Isolation and efficacy of two bacterial strains antagonists of Erwinia amylovora and Pectobacterium carotovorum

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
M’hamed BENADA ◽  
Boualem BOUMAAZA ◽  
Sofiane BOUDALIA ◽  
Omar KHALADI
Keyword(s):  
Fire Blight ◽  
Erwinia Amylovora ◽  
Bacterial Species ◽  
Crop Protection ◽  
Soft Rot ◽  
Causal Agent ◽  
Plant Diseases ◽  
Ecological Niches ◽  
Pectobacterium Carotovorum ◽  
Bacterial Strains

Abstract Background The development of ecofriendly tools against plant diseases is an important issue in crop protection. Screening and selection process of bacterial strains antagonists of 2 pathogenic bacterial species that limit very important crops, Erwinia amylovora, the causal agent of the fire blight disease, and Pectobacterium carotovorum, the causal agent of bacterial potato soft rot, were reported. Bacterial colonies were isolated from different ecological niches, where both pathogens were found: rhizosphere of potato tubers and fruits and leaves of pear trees from the northwest region of Algeria. Direct and indirect confrontation tests against strains of E. amylovora and P. carotovorum were performed. Results Results showed a significant antagonistic activity against both phytopathogenic species, using direct confrontation method and supernatants of cultures (p<0.005). In vitro assays showed growth inhibitions of both phytopathogenic species. Furthermore, results revealed that the strains of S. plymuthica had a better inhibitory effect than the strains of P. fluorescens against both pathogens. In vivo results on immature pear fruits showed a significant decrease in the progression of the fire blight symptoms, with a variation in the infection index from one antagonistic strain to another between 31.3 and 50%, and slice of potato showed total inhibition of the pathogen (P. carotovorum) by the antagonistic strains of Serratia plymuthica (p<0.005). Conclusion This study highlighted that the effective bacteria did not show any infection signs towards plant tissue, and considered as a potential strategy to limit the fire blight and soft rot diseases.

scholarly journals First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia

Plant Disease ◽  
2019 ◽  
Vol 103 (10) ◽  
pp. 2667-2667 ◽  
Author(s):  
N. Zlatković ◽  
A. Prokić ◽  
K. Gašić ◽  
N. Kuzmanović ◽  
M. Ivanović ◽  
...  
Keyword(s):  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
First Report ◽  
Carotovorum Subsp

scholarly journals An In Vitro Study on the Antimicrobial Properties of Essential Oil Modified Resin Composite against Oral Pathogens

Materials ◽  
2020 ◽  
Vol 13 (19) ◽  
pp. 4383
Author(s):  
Barbara Lapinska ◽  
Aleksandra Szram ◽  
Beata Zarzycka ◽  
Janina Grzegorczyk ◽  
Louis Hardan ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antifungal Activity ◽  
Antimicrobial Agents ◽  
Resin Composite ◽  
Antimicrobial Properties ◽  
In Vitro Study ◽  
Diffusion Method ◽  
Oral Pathogens

Modifying the composition of dental restorative materials with antimicrobial agents might induce their antibacterial potential against cariogenic bacteria, e.g., S.mutans and L.acidophilus, as well as antifungal effect on C.albicans that are major oral pathogens. Essential oils (EOs) are widely known for antimicrobial activity and are successfully used in dental industry. The study aimed at evaluating antibacterial and antifungal activity of EOs and composite resin material (CR) modified with EO against oral pathogens. Ten EOs (i.e., anise, cinnamon, citronella, clove, geranium, lavender, limette, mint, rosemary thyme) were tested using agar diffusion method. Cinnamon and thyme EOs showed significantly highest antibacterial activity against S.mutans and L.acidophilus among all tested EOs. Anise and limette EOs showed no antibacterial activity against S.mutans. All tested EOs exhibited antifungal activity against C.albicans, whereas cinnamon EO showed significantly highest and limette EO significantly lowest activity. Next, 1, 2 or 5 µL of cinnamon EO was introduced into 2 g of CR and microbiologically tested. The modified CR showed higher antimicrobial activity in comparison to unmodified one. CR containing 2 µL of EO showed the best antimicrobial properties against S.mutans and C.albicans, while CR modified with 1 µL of EO showed the best antimicrobial properties against L.acidophilus.

Antibacterial Activity of Nano-Structured TiO2Surfaces: a Preliminary in Vitro Study

2010 ◽  
pp. 163-172
Author(s):  
Luigi de Nardo ◽  
Ilaria Alfieri ◽  
Andrea Lorenzi ◽  
Enrica Saino ◽  
Livia Visai ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals The Ribosomal Protein RplY Is Required for Pectobacterium carotovorum Virulence and Is Induced by Zantedeschia elliotiana Extract

2017 ◽  
Vol 107 (11) ◽  
pp. 1322-1330 ◽  
Author(s):  
Huan Jiang ◽  
Mengyi Jiang ◽  
Liuke Yang ◽  
Peiyan Yao ◽  
Lin Ma ◽  
...  
Keyword(s):  
Plant Extract ◽  
Plant Cell Wall ◽  
Insertion Site ◽  
Bacterial Pathogen ◽  
Soft Rot ◽  
Kanamycin Resistance ◽  
Pectobacterium Carotovorum ◽  
Promoter Trap ◽  
Carotovorum Subsp ◽  
Rot Disease

Pectobacterium carotovorum subsp. carotovorum strain PccS1, a bacterial pathogen causing soft rot disease of Zantedeschia elliotiana (colored calla), was investigated for virulence genes induced by the host plant. Using a promoter-trap transposon (mariner), we obtained 500 transposon mutants showing kanamycin resistance dependent on extract of Z. elliotiana. One of these mutants, PM86, exhibited attenuated virulence on both Z. elliotiana and Brassica rapa subsp. pekinensis. The growth of PM86 was also reduced in minimal medium (MM), and the reduction was restored by adding plant extract to the MM. The gene containing the insertion site was identified as rplY. The deletion mutant ΔrplY, exhibited reduced virulence, motility and plant cell wall-degrading enzyme production but not biofilm formation. Analysis of gene expression and reporter fusions revealed that the rplY gene in PccS1 is up-regulated at both the transcriptional and the translational levels in the presence of plant extract. Our results suggest that rplY is induced by Z. elliotiana extract and is crucial for virulence in P. carotovorum subsp. carotovorum.

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