scholarly journals Determination of resistance and virulence genes in Enterococcus faecalis and E. faecium strains isolated from poultry and their genotypic characterization by ADSRRS-fingerprinting

2017 ◽  
Vol 96 (4) ◽  
pp. 986-996 ◽  
Author(s):  
A. Nowakiewicz ◽  
G. Ziółkowska ◽  
A. Trościańczyk ◽  
P. Zięba ◽  
S. Gnat
Keyword(s):  
Enterococcus Faecalis ◽  
Virulence Genes ◽  
Genotypic Characterization

Detection of virulence factors in high-level gentamicin-resistant Enterococcus faecalis and Enterococcus faecium isolates from a Tunisian hospital

2007 ◽  
Vol 53 (3) ◽  
pp. 372-379 ◽  
Author(s):  
N. Klibi ◽  
K. Ben Slama ◽  
Y. Sáenz ◽  
A. Masmoudi ◽  
S. Zanetti ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Virulence Factors ◽  
Enterococcus Faecium ◽  
Biofilm Formation ◽  
Virulence Genes ◽  
Virulence Gene ◽  
Gelatinase Activity ◽  
Genes Encoding ◽  
High Level

Phenotypic and genotypic determination of virulence factors were carried out in 46 high-level gentamicin-resistant (HLGR) clinical Enterococcus faecalis (n = 34) and Enterococcus faecium (n = 12) isolates recovered from different patients in La Rabta Hospital in Tunis, Tunisia, between 2000 and 2003 (all these isolates harboured the aac(6′)–aph(2″) gene). The genes encoding virulence factors (agg, gelE, ace, cylLLS, esp, cpd, and fsrB) were analysed by PCR and sequencing. The production of gelatinase and hemolysin, the adherence to caco-2 and hep-2 cells, and the capacity for biofilm formation were investigated in all 46 HLGR enterococci. The percentages of E. faecalis isolates harbouring virulence genes were as follows: gelE, cpd, and ace (100%); fsrB (62%); agg (56%); cylLLS (41.2%); and esp (26.5%). The only virulence gene detected among the 12 HLGR E. faecium isolates was esp (58%). Gelatinase activity was detected in 22 of the 34 E. faecalis isolates (65%, most of them with the gelE+–fsrB+ genotype); the remaining 12 isolates were gelatinase-negative (with the gelE+–fsrB– genotype and the deletion of a 23.9 kb fragment of the fsr locus). Overall, 64% of the cylLLS-containing E. faecalis isolates showed β-hemolysis. A high proportion of our HLGR E. faecalis isolates, in contrast to E. faecium, showed moderate or strong biofilm formation or adherence to caco-2 and hep-2 cells.

Relation of Enterococcus faecalis and Enterococcus faecium Isolates from Foods and Clinical Specimens

2008 ◽  
Vol 71 (10) ◽  
pp. 2100-2104 ◽  
Author(s):  
S. P. TEMPLER ◽  
P. ROHNER ◽  
A. BAUMGARTNER
Keyword(s):  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Virulence Genes ◽  
Antimicrobial Agents ◽  
Patient Specific ◽  
Pfge Typing ◽  
Resistance Patterns ◽  
Small Clusters ◽  
Pfge Profiles

Clinical Enterococcus faecalis (n = 65) and Enterococcus faecium (n = 12) blood isolates from three Swiss hospitals were characterized with testing for resistance to antimicrobial agents, pulsed-field gel electrophoresis (PFGE), and the occurrence of virulence factors. Phenotypic determination of resistance to antimicrobial agents resulted in 20% of E. faecalis isolates showing a triple resistance against chloramphenicol, tetracycline, erythromycin, and seven isolates (two E. faecalis and five E. faecium) exhibiting a multiresistance against five or more antimicrobials. One isolate each of E. faecalis and E. faecium showed vancomycin resistance. All isolates contained at least two of the nine tested virulence genes (agg, gelE, cyl, esp, efaAfs, efaAfm, cpd, cob, and ccf ). Phylogenetic analysis of the PFGE profiles identified several small clusters within E. faecalis isolates, one of which included isolates of all three hospitals. Fifty-six (73%) isolates occurred as unique, patient-specific clones. Several PFGE types were associated with shared features in their resistance patterns, indicating spread between and within wards. Finally, enterococci from this study and previous isolates from cheeses were examined by PFGE typing. The comparison of PFGE profiles from human and food isolates resulted in clusters of genetically strong related strains, which suggests high similarities of the enterococcal community composition of these two environments. A possible spread of the enterococcal isolates through the food supply cannot be excluded.

Complete Genome Sequence of Bacteriophage EFC1 Infecting Enterococcus Faecalis From Chicken

2021 ◽  
Author(s):  
Qi Wang ◽  
Na Liu
Keyword(s):  
Enterococcus Faecalis ◽  
Virulence Genes ◽  
Large Subunit ◽  
Antibiotic Resistance Genes ◽  
Lytic Phage ◽  
High Similarity ◽  
Protein Coding ◽  
Circular Dna ◽  
Intron Structure ◽  
Comparative Phylogenetic Analysis

Abstract In response to Enterococcus faecalis infection of chicken origin, a multi host lytic phage, EFC1 was isolated and characterized the double-stranded circular DNA genome with size of 56099 bp, containing 89 predicted protein coding genes as well as 2 tRNAs involved in intron, structure, transcription, packaging, DNA replication, modification, lysis. Observation of the structure by electron microscopy and comparative phylogenetic analysis of terminase large subunit showed that the phage EFC1 belongs to a new member of Siphoviridae, which is relatively distantly related to its high similarity phages. The phage EFC1 has no relevant virulence genes and antibiotic resistance genes.

Antibiotic Resistance and Virulence Genes in Enterococcus faecalis Isolated From Human Dental Plaque

2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Mehrnaz Bakhti ◽  
Mona Akhondnezhad ◽  
Mehrdad Gholami ◽  
Mohtaram Nasrolahei ◽  
Hamid Reza Goli
Keyword(s):  
Antibiotic Resistance ◽  
Enterococcus Faecalis ◽  
Dental Plaque ◽  
Virulence Genes

scholarly journals Factors influencing determination of high-level aminoglycoside resistance in Enterococcus faecalis.

1991 ◽  
Vol 29 (9) ◽  
pp. 1934-1939 ◽  
Author(s):  
D F Sahm ◽  
S Boonlayangoor ◽  
P C Iwen ◽  
J L Baade ◽  
G L Woods
Keyword(s):  
Enterococcus Faecalis ◽  
Aminoglycoside Resistance ◽  
Factors Influencing ◽  
High Level

Occurrence of virulence-associated genes in clinical Enterococcus faecalis strains isolated in Londrina, Brazil

2004 ◽  
Vol 53 (11) ◽  
pp. 1069-1073 ◽  
Author(s):  
Elisa Bittencourt de Marques ◽  
Sérgio Suzart
Keyword(s):  
Enterococcus Faecalis ◽  
Virulence Genes ◽  
Epidemiological Studies ◽  
Wide Distribution ◽  
Virulence Determinants ◽  
Simultaneous Presence ◽  
Common Pattern ◽  
Clinical Strains ◽  
Virulence Markers ◽  
Serious Infections

Epidemiological studies have reinforced the importance of Enterococcus faecalis in causing serious infections, and to date, our understanding of how certain virulence factors are involved in the pathogenesis of enterococcal infections is still limited. The aim of the present study was to examine the occurrence of known virulence determinants in a group of E. faecalis strains isolated from different clinical sources in Brazil. A total of 95 E. faecalis strains were investigated for the presence of nine virulence genes including aggA, cylA, cylB, cylM, eep, efaA, enlA, esp and gelE by using PCR. The data showed a relatively wide distribution of the virulence genes among the investigated strains. The clinical strains carried at least one and concomitantly up to as many as eight virulence markers, with two or three being the most common pattern. Most of the strains carried efaA (58.9 %), eep (58.9 %) and esp (57.9 %) genes, whereas the remaining virulence markers were detected in variable percentages ranging from 9.5 to 45 %. Simultaneous presence of virulence markers was observed among clinical strains regardless of their sources. In this study, the efaA + esp + gelE + profile was the virulence genotype most frequently detected among E. faecalis strains. Finally, there was no significant association between virulence markers and clinical sources.

scholarly journals Genetic relatedness of the Enterococcus faecalis isolates in stool and urine samples of patients with community-acquired urinary tract infection

Gut Pathogens ◽  
2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Zohreh Ghalavand ◽  
Masoud Alebouyeh ◽  
Kiandokht Ghanati ◽  
Leila Azimi ◽  
Marjan Rashidan
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Enterococcus Faecalis ◽  
Intestinal Tract ◽  
Virulence Genes ◽  
Genetic Relatedness ◽  
Molecular Fingerprints ◽  
Rapd Pcr ◽  
Mic Value

Abstract Background Community-acquired urinary tract infection (CA-UTI) could be caused by endogenous or exogenous routes. To show this relationship, we investigated molecular fingerprints and genotypes of paired Enterococcus faecalis isolated from the urine of symptomatic patients and their fecal samples. Results Out of the studied patients, 63 pairs of E. faecalis isolates were obtained simultaneously from their urine and feces samples. All the strains were sensitive to vancomycin, linezolid, nitrofurantoin, and daptomycin (MIC value: ≤ 4 µg/ml), while resistance to tetracycline (urine: 88.9%; stool: 76.2%) and minocycline (urine: 87.3%, stool: 71.4%) was detected in most of them. The most common detected virulence genes were included efbA, ace, and gelE. RAPD-PCR and PFGE analyses showed the same patterns of molecular fingerprints between paired of the isolates in 26.9% and 15.8% of the patients, respectively. Conclusions Similarity of E. faecalis strains between the urine and feces samples confirmed the occurrence of endogenous infection via contamination with colonized bacteria in the intestinal tract. Carriage of a complete virulence genotype in the responsible strains was statistically in correlation with endogenous UTI, which shows their possible involvement in pathogenicity of uropathogenic E. faecalis strains.

scholarly journals Detection of pathogenic Yersinia enterocolitica serotype O:3 by biochemical, serological, and PCR methods

2008 ◽  
Vol 25 (No. 4) ◽  
pp. 214-220 ◽  
Author(s):  
J. Simonová ◽  
M. Vázlerová ◽  
I. Steinhauserová
Keyword(s):  
Czech Republic ◽  
Yersinia Enterocolitica ◽  
Virulence Genes ◽  
Optimum Time ◽  
The Czech Republic ◽  
Specific Identification ◽  
Time Consumption ◽  
Serotype O

In this study, the pathogenic <i>Y. enterocolitica</i> of serotype O:3 was monitored. The serotype is widely spread in Europe and has been linked to human yersiniosis. For the detection of pathogenic strains were used biochemical and serological methods as well as PCR methods based on the identification of virulence genes (<i>ail</i>, <i>rfbC</i>, <i>ystA</i>, <i>yadA</i>, <i>virF</i>). The occurrence of <i>Y. enterocolitica</i> O:3 strains was monitored in slaughter animals from a number of farms in the Czech Republic. A total of 3748 samples were collected coming from pigs (1388), cattle (633), poultry (902), and slaughter facilities (825). Fifty-two <i>Y. enterocolitica</i> O:3 isolates were identified by biochemical and serologic methods, and 53 <i>Y. enterocolitica</i> O:3 isolates were identified by PCR methods (46 isolates from pigs, 2 isolates from poultry, 3 isolates from cattle, and 2 isolates from a poultry slaughtering facility). All isolates of <i>Y. enterocolitica</i> O:3 carried genes <i>ail</i> and <i>rfbC</i>, 83% isolates carried gene <i>ystA</i>, 79% isolates carried gene <i>yadA</i> and 49% isolates carried gene <i>virF</i>. The use of PCR methods based on the identification of <i>ail</i> and <i>rfbC</i> genes provides for a sufficiently specific identification of pathogenic <i>Y. enterocolitica</i> O:3 strains with optimum time consumption compared to biochemical and serological methods. It is not recommendable to use other PCR methods (detection of the <i>ystA, <i>yadA</i>, and <i>virF</i> genes) for the detection of pathogenic <i>Y. enterocolitica</i> strains because those methods are not very specific for the determination of pathogenicity.

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