scholarly journals A Developmental Engineering-Based Approach to Bone Repair: Endochondral Priming Enhances Vascularization and New Bone Formation in a Critical Size Defect

2020 ◽  
Vol 8 ◽  
Author(s):  
Fiona E. Freeman ◽  
Meadhbh Á. Brennan ◽  
David C. Browe ◽  
Audrey Renaud ◽  
Julien De Lima ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Repair ◽  
Critical Size ◽  
New Bone Formation ◽  
Critical Size Defect ◽  
Developmental Engineering

3D scaffold induces efficient bone repair: in vivo studies of ultra-structural architecture at the interface

RSC Advances ◽  
2016 ◽  
Vol 6 (96) ◽  
pp. 93768-93776 ◽  
Author(s):  
Nitin Sagar ◽  
Atul Kumar Singh ◽  
Mayur K. Temgire ◽  
S. Vijayalakshmi ◽  
Alok Dhawan ◽  
...  
Keyword(s):  
Growth Factor ◽  
Bone Formation ◽  
Bone Repair ◽  
Critical Size ◽  
In Vivo Studies ◽  
New Bone Formation ◽  
3D Scaffold ◽  
Critical Size Defect ◽  
Structural Architecture

Biodegradable designer 3D scaffold bridges critical size defect and induces new bone formation as revealed by ssNMR, SEM, EDX and μ-CT in rabbit tibial model during healing. No growth factor was needed due to chemical and microstructural cues.

scholarly journals Lack of repair of rat skull critical size defect treated with bovine morphometric protein bound to microgranular bioabsorbable hydroxyapatite

2004 ◽  
Vol 15 (3) ◽  
pp. 175-180 ◽  
Author(s):  
Gabriel Ramalho Ferreira ◽  
Tania Mary Cestari ◽  
José Mauro Granjeiro ◽  
Rumio Taga
Keyword(s):  
Foreign Body ◽  
Bone Formation ◽  
Bone Morphogenetic Proteins ◽  
Bone Repair ◽  
Critical Size ◽  
Bovine Bone ◽  
New Bone Formation ◽  
Body Type ◽  
Granulomatous Reaction ◽  
Post Surgery

The ability of a pool of bovine bone morphogenetic proteins bound to synthetic microgranular hydroxyapatite (BMPb-HA) to stimulate bone repair was determined in rat critical size defects. An 8-mm diameter defect was created in the calvaria of 25 rats. In 15 rats, the defects were filled with BMPb-HA homogenized with blood (experimental group), and in 10 rats the defects were filled only with blood clots (control). The calvariae of experimental rats were collected 1, 3 and 6 months after surgery and of the control rats at the end of surgery and 6 months thereafter. The morphometric results obtained in the radiographs showed an absence of new bone formation at 1 and 3 months post-surgery and, histologically, the defects were filled with fibrous connective tissue and numerous foci of a foreign body-type granulomatous reaction around hydroxyapatite agglomerates. At the end of 6 months, the number and size of the granulomatous foci decreased and the area of the defects was reduced by 22% compared to the 0-hour control due to the formation of new bone at their borders, although the mean area was similar to the 6-month control. We conclude that the use of BMPb-HA in the treatment of critical size bone defects of the rat skull leads to the formation of a foreign body-type granulomatous reaction that markedly inhibits new bone formation, suggesting that synthetic microgranular hydroxyapatite does not represent a good carrier for BMP-induced bone formation.

scholarly journals Osseous Healing in Surgically Prepared Bone Defects Using Different Grafting Materials: An Experimental Study in Pigs

2020 ◽  
Vol 8 (1) ◽  
pp. 7 ◽  
Author(s):  
Savvas Titsinides ◽  
Theodore Karatzas ◽  
Despoina Perrea ◽  
Efstathios Eleftheriadis ◽  
Leonidas Podaropoulos ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Repair ◽  
Healing Process ◽  
Bone Substitutes ◽  
Bone Defects ◽  
New Bone Formation ◽  
Post Surgery ◽  
Beta Tricalcium Phosphate ◽  
Close Proximity ◽  
Tissue Specimens

Regeneration of large jaw bone defects still remains a clinical challenge. To avoid incomplete bone repair, bone grafts have been advocated to support the healing process. This study comparatively evaluated new bone formation among a synthetic graft substitute, a human bone derivative, and a bovine xenograft. Materials were placed in 3 out of the 4 bone cavities, while 1 deficit was left empty, serving as a control, in mono-cortical defects, surgically prepared in the porcine calvaria bone. Animals were randomized in 2 groups and euthanized at 8 and 12 weeks. Harvested tissue specimens were qualitatively evaluated by histology. New bone formation was quantitatively measured by histomorphometry. Maximum new bone formation was noticed in defects grafted with beta-tricalcium phosphate b-TCP compared to the other bone substitutes, at 8 and 12 weeks post-surgery. Bovine and human allograft induced less new bone formation compared to empty bone cavity. Histologic analysis revealed that b-TCP was absorbed and substituted significantly, while bovine and human allograft was maintained almost intact in close proximity with new bone. Based on our findings, higher new bone formation was detected in defects filled with b-TCP when compared to bovine and human graft substitutes.

Collagen/glycosaminoglycan coatings enhance new bone formation in a critical size bone defect — A pilot study in rats

2017 ◽  
Vol 71 ◽  
pp. 84-92 ◽  
Author(s):  
Yvonne Förster ◽  
Ricardo Bernhardt ◽  
Vera Hintze ◽  
Stephanie Möller ◽  
Matthias Schnabelrauch ◽  
...  
Keyword(s):  
Pilot Study ◽  
Bone Formation ◽  
Bone Defect ◽  
Critical Size ◽  
New Bone Formation

Bone Marrow Autograft Associated to Macroporous Biphasic Calcium Phosphate for Bone Substitution in an Animal Model of Sequels of Radiotherapy

2005 ◽  
Vol 284-286 ◽  
pp. 285-288
Author(s):  
Oliver Malard ◽  
Jean Michel Bouler ◽  
Jerome Guicheux ◽  
Olivier Gauthier ◽  
E. Lerouxel ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Calcium Phosphate ◽  
Bone Formation ◽  
Biphasic Calcium Phosphate ◽  
Bone Repair ◽  
Major Surgery ◽  
Graft Versus Host Reaction ◽  
Bone Reconstruction ◽  
New Bone Formation ◽  
Study Results

Bone invasion is common in case of Squamous Cell Carcinomas (SCC) of the upper aero-digestive tract. Radiotherapy is required in addition to large surgical tumor removal. This treatment usually generates irreversible injuries on the reparation properties of the tissues, especially on bone. The quality of life of patients undergoing major surgery and radiotherapy in maxillary and mandible areas is reduced, but could be improved by bone reconstruction. The aim of this study was to evaluate the bone reconstruction possibilities by Macroporous Biphasic Calcium-Phosphate (MBCPÔ). The MBCP substitute was evaluated as granules and associated to autologous bone marrow (BM) graft in irradiated areas, in an inbreeding rodent model. Radiation sequels were created on inferior members of half of the rats. 3 weeks later, 3-mm osseous defects were created on each animal. The inbreeding model allows BM to be grafted without graft-versus-host reaction. Defects were filled either with MBCP alone, BM alone or a mixture of MBCP and BM. Six weeks after implantation, animals were sacrificed: bone repair and ceramic degradation were evaluated by qualitative and quantitative study. Results showed that bioceramics were well osteointegrated. Filling the defects with BM alone showed a significant increased of newly-formed bone formation but only after irradiation, whereas filling defects with MBCP alone increased new-bone formation only without previous irradiation. Associating MBCP to BM provided the best new-bone formation rates after irradiation. Degradation of the ceramic was the most important in case of BM grafting. This study demonstrated that BM added to MBCP constitute an appropriate material to be considered in case of bone defect occurring in irradiated tissue, and could be foreseen for use after bone removal for oncologic obligations.

Effect of Silicon-Doped Calcium Phosphate Bone Substitutes on Bone Formation and Osteoblastic Phenotype Expression In Vivo

2014 ◽  
Vol 614 ◽  
pp. 31-34 ◽  
Author(s):  
Christine Knabe ◽  
Marco Lopez Heredia ◽  
Dirk Barnemitz ◽  
Antje Genzel ◽  
Fabian Peters ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Bone Substitute ◽  
Bone Repair ◽  
Critical Size ◽  
Potassium Phosphate ◽  
Critical Size Defects ◽  
Bone Substitute Materials ◽  
Silicon Doped ◽  
Substitute Materials

This study evaluates the effect of two novel particulate silicon-doped calcium phosphate graft materials as compared to the currently clinically used material β-TCP on osteogenesis and bone formation after implantation in critical-size defects the sheep scapula. These materials were developed in order to create biodegradable bone substitute materials that degrade rapidly, but still stimulate osteogenesis at the same time, thereby resulting in bone repair and regeneration with fully functional bone tissue. All bone substitute materials studied facilitated excellent bony regeneration of critical-size defects in the sheep scapula. Of the three grafting materials studied, the calcium alkali orthophosphate material with the crystalline phase Ca2KNa (PO4)2, with a small amorphous portion containing magnesium potassium phosphate and a small addition of sodium magnesium silicate had the greatest stimulatory effect on bone formation and expression of osteogenic markers, while exhibiting the highest biodegradability.

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