scholarly journals Heterotrophically Ultrahigh-Cell-Density Cultivation of a High Protein-Yielding Unicellular Alga Chlorella With a Novel Nitrogen-Supply Strategy

Author(s):  
Quan Xu ◽  
Guoli Hou ◽  
Jianping Chen ◽  
Hongxia Wang ◽  
Li Yuan ◽  
...  

The unicellular green alga Chlorella is an ideal protein source. However, the high production cost and low production capability of the current main photoautotrophic culture mode limit its application especially as an alternative protein source for food and feed, which might be overcome through high-cell-density cultivation in fermenters. In this study, a Chlorella sorokiniana strain CMBB276 with high protein content was selected from five Chlorella strains by comprehensive evaluation of their growth rates, protein contents, and yields. The optimal cultural temperature, pH, and mole ratio of carbon and nitrogen (C/N) for C. sorokiniana CMBB276 growth were found to be 30°C, 6.5, and 18, respectively. Ammonium chloride was proved to be the best nitrogen (N) source for C. sorokiniana CMBB276 growth, whereas growth inhibition caused by the accumulation of salts was observed under fed-batch cultivation when maintaining a constant C/N ratio of 18 by controlling pH with sodium hydroxide solution. By simultaneously reducing the concentration of ammonium chloride in the feeding medium and controlling pH with ammonium hydroxide, we finally achieved the ultrahigh-cell-density cultivation of C. sorokiniana CMBB276. The highest biomass concentration and protein yield reached 232 and 86.55 g l−1, respectively, showing the great potential of culturing C. sorokiniana CMBB276 in fermenters for economic and large-scale protein source production.

2018 ◽  
pp. 172-182 ◽  
Author(s):  
Shengmin CAO

This paper mainly studies the application of intelligent lighting control system in different sports events in large sports competition venues. We take the Xiantao Stadium, a large­scale sports competition venue in Zaozhuang City, Shandong Province as an example, to study its intelligent lighting control system. In this paper, the PID (proportion – integral – derivative) incremental control model and the Karatsuba multiplication model are used, and the intelligent lighting control system is designed and implemented by multi­level fuzzy comprehensive evaluation model. Finally, the paper evaluates the actual effect of the intelligent lighting control system. The research shows that the intelligent lighting control system designed in this paper can accurately control the lighting of different sports in large stadiums. The research in this paper has important practical significance for the planning and design of large­scale sports competition venues.


Technologies ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 28
Author(s):  
Hossam A. Gabbar ◽  
Ahmed M. Othman ◽  
Muhammad R. Abdussami

The evolving global landscape for electrical distribution and use created a need area for energy storage systems (ESS), making them among the fastest growing electrical power system products. A key element in any energy storage system is the capability to monitor, control, and optimize performance of an individual or multiple battery modules in an energy storage system and the ability to control the disconnection of the module(s) from the system in the event of abnormal conditions. This management scheme is known as “battery management system (BMS)”, which is one of the essential units in electrical equipment. BMS reacts with external events, as well with as an internal event. It is used to improve the battery performance with proper safety measures within a system. Therefore, a safe BMS is the prerequisite for operating an electrical system. This report analyzes the details of BMS for electric transportation and large-scale (stationary) energy storage. The analysis includes different aspects of BMS covering testing, component, functionalities, topology, operation, architecture, and BMS safety aspects. Additionally, current related standards and codes related to BMS are also reviewed. The report investigates BMS safety aspects, battery technology, regulation needs, and offer recommendations. It further studies current gaps in respect to the safety requirements and performance requirements of BMS by focusing mainly on the electric transportation and stationary application. The report further provides a framework for developing a new standard on BMS, especially on BMS safety and operational risk. In conclusion, four main areas of (1) BMS construction, (2) Operation Parameters, (3) BMS Integration, and (4) Installation for improvement of BMS safety and performance are identified, and detailed recommendations were provided for each area. It is recommended that a technical review of the BMS be performed for transportation electrification and large-scale (stationary) applications. A comprehensive evaluation of the components, architectures, and safety risks applicable to BMS operation is also presented.


Adipocyte ◽  
2016 ◽  
Vol 5 (2) ◽  
pp. 196-211 ◽  
Author(s):  
Ulrike Liisberg ◽  
Lene Secher Myrmel ◽  
Even Fjære ◽  
Alexander K. Rønnevik ◽  
Susanne Bjelland ◽  
...  

Author(s):  
Jason J. Saleem ◽  
Kyle Maddox ◽  
Jennifer Herout ◽  
Kurt Ruark

This practice-oriented paper presents a human-centered design (HCD) framework that we developed to perform a comprehensive evaluation of a new health information technology (HIT) system under development, intended to replace a legacy system. The Department of Veterans Affairs (VA) Veteran Crisis Line (VCL) program provides a vital service in crisis intervention and suicide prevention. VCL staff rely on a Customer Relationship Management (CRM) legacy system, Medora. VCL intended to replace Medora with Microsoft Dynamics 365 (D365) CRM system. Due to wide-spread criticism of D365, the VA Human Factors Engineering (HFE) team engaged in a multi-study, mixed-method HCD evaluation to investigate the legacy system and intended replacement in terms of ability to support VCL staff needs. The HCD framework we developed to perform this evaluation may be adapted for other large-scale HIT transitions and may provide human factors practitioners with guidance to make evidence-based decisions to support (or abandon) such transitions.


2018 ◽  
Vol 188 ◽  
pp. 05004
Author(s):  
Christos Panagiotou ◽  
Christos Antonopoulos ◽  
Stavros Koubias

WSNs as adopted in current smart city deployments, must address demanding traffic factors and resilience in failures. Furthermore, caching of data in WSN can significantly benefit resource conservation and network performance. However, data sources generate data volumes that could not fit in the restricted data cache resources of the caching nodes. This unavoidably leads to data items been evicted and replaced. This paper aims to experimentally evaluate the prominent caching techniques in large scale networks that resemble the Smart city paradigm regarding network performance with respect to critical application and network parameters. Through respective result analysis valuable insights are provided concerning the behaviour of caching in typical large scale WSN scenarios.


2021 ◽  
Vol 27 (spe) ◽  
pp. 56-58
Author(s):  
Pei Jiang

ABSTRACT The comprehensive performance evaluation system of public sports service is an important part of public sports service system in China. How to objectively and comprehensively evaluate the present situation of public sports service performance in our country has always been a difficult problem in the development of public sports service. Based on the five principles of constructing the recipient satisfaction index, the PCSSI model of the comprehensive evaluation system of public sports service supply was constructed using scale design. Large-scale stadiums in seven districts of Shenyang were selected for investigation. The recipient satisfaction index model was established to compare the satisfaction index of the 7 regions, and analyze the influencing factors of the satisfaction index of public sports service supply, and put forward some suggestions for improving the service level of large stadiums and gymnasiums in Shenyang. Practice has proved that the application of the PCSSI model has a positive effect on improving the performance level of public sports service supply in China.


2019 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Aam Gunawan

Maggot black soldier fly (Hermetia illucens) is a protein source of feed which is highly favored by poultry, especially ducks and chickens. However, it is feared that the provision of live maggot in ducks will affect the organoleptic quality of the egg, especially its taste and aroma. Therefore this study aims to determine the organoleptic quality of duck eggs fed Hermetia illucens maggot feeds in a living state. The study used 120 alabio ducks which were placed in a postal cage. The design used was a completely randomized design, each treatment using six replications. Each replication is taken egg sample to be tested panelists. The panelists used were 67 panelists who were somewhat trained. The data obtained were analyzed of variance and Duncan's multiple range test. The treatments that were tried consisted of P1: low protein rations without live maggot, P2: low protein rations with live maggot administration 40 g/bird/day, P3: high protein rations without live maggot administration, and P4: high protein rations with live maggot administration 40g/bird/day. The results showed that the treatment affected the texture, flavor, and aroma of boiled eggs. Ducks fed with high protein ration coupled with the provision of live maggot 40 g/bird/day produce softer textures, tastes quite good, and aroma more fishy. Keywords: Maggot, duck eggs, organoleptics


Atmosphere ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1538
Author(s):  
Lijun Xu ◽  
Qian Liu ◽  
Yingying Nie ◽  
Feng Li ◽  
Guixia Yang ◽  
...  

Integration of perennial grass species into the current food production systems, especially in the agropastoral regions worldwide, may produce multiple benefits including, among others, a more stable productivity and a smaller eco-environmental footprint. However, one of the fundamental challenges facing the large-scale adoption of such grass species is their ability to withstand the vagaries of winter in these regions. Here, we present a comprehensive evaluation of the winter hardiness of 50 indigenous Chinese cultivars of alfalfa, a high-quality leguminous perennial grass, in comparison with six introduced U.S. cultivars in a multi-site field experiment in northern China. Our results reveal that indigenous cultivars have stronger winter hardiness than introduced cultivars. Cultivars native in the north performed better than southern cultivars, suggesting that suitability evaluation is an unavoidable step proceeding any regional implementations. Our results also show that the metric we used to assess alfalfa’s winter hardiness, the average score index (ASI), produced more consistent results than another more-widely used metric of winter survival rate (WSR). These findings offer a systematic field evidence that supports regional cropping system adjustment and production system betterment to ensure food security under climate change in the region and beyond.


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