scholarly journals Photothermal Detection of MicroRNA Using a Horseradish Peroxidase-Encapsulated DNA Hydrogel With a Portable Thermometer

2021 ◽  
Vol 9 ◽  
Author(s):  
Xiujuan Liu ◽  
Meixiang Zhang ◽  
Ze Chen ◽  
Jiuqing Cui ◽  
Long Yang ◽  
...  
Keyword(s):  
Horseradish Peroxidase ◽  
Near Infrared ◽  
Color Change ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Absorption Spectrometry ◽  
Infrared Light ◽  
Photothermal Effect ◽  
Temperature Sensitive ◽  
Dna Hydrogel

MicroRNA (miRNA) detection has attracted widespread interest as a tumor detection marker. In this work, a miRNA-responsive visual and temperature sensitive probe composed of a horseradish peroxidase (HRP)-encapsulated DNA hydrogel was designed and synthesized. The biosensor converted the miRNA hybridization signal to a photothermal effect which was measured using a digital thermometer. The substrate DNA linker strand of the hydrogel hybridizes with different sequences of miRNA resulting in the collapse of the hydrogel and the release of HRP. HRP oxidizes 3,3′,5,5′-tetramethylbenzidine (TMB) resulting in a color change and a strong photothermal effect was observed after shining near-infrared light on the oxidized product. The thermometer-based readout method has a wide linear range (0.5–4.0 µM) and a limit of detection limit of 7.8 nM which is comparable with traditional UV-vis absorption spectrometry detection and quantitative real time polymerase chain reaction methods. The low cost, ease of operation, and high sensitivity shows that this biosensor has potential for point-of-care biomolecular detection and biomedical applications.

scholarly journals Boosting Chemodynamic Therapy by the Synergistic Effect of Co-Catalyze and Photothermal Effect Triggered by the Second Near-Infrared Light

2020 ◽  
Vol 12 (1) ◽  
Author(s):  
Songtao Zhang ◽  
Longhai Jin ◽  
Jianhua Liu ◽  
Yang Liu ◽  
Tianqi Zhang ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Bovine Serum Albumin ◽  
Synergistic Effect ◽  
Bovine Serum ◽  
Photothermal Therapy ◽  
Near Infrared ◽  
Infrared Light ◽  
Photothermal Effect ◽  
Reaction Efficiency

AbstractIn spite of the tumor microenvironments responsive cancer therapy based on Fenton reaction (i.e., chemodynamic therapy, CDT) has been attracted more attentions in recent years, the limited Fenton reaction efficiency is the important obstacle to further application in clinic. Herein, we synthesized novel FeO/MoS2 nanocomposites modified by bovine serum albumin (FeO/MoS2-BSA) with boosted Fenton reaction efficiency by the synergistic effect of co-catalyze and photothermal effect of MoS2 nanosheets triggered by the second near-infrared (NIR II) light. In the tumor microenvironments, the MoS2 nanosheets not only can accelerate the conversion of Fe3+ ions to Fe2+ ions by Mo4+ ions on their surface to improve Fenton reaction efficiency, but also endow FeO/MoS2-BSA with good photothermal performances for photothermal-enhanced CDT and photothermal therapy (PTT). Consequently, benefiting from the synergetic-enhanced CDT/PTT, the tumors are eradicated completely in vivo. This work provides innovative synergistic strategy for constructing nanocomposites for highly efficient CDT.

In vivo Monitoring of Gold Nanorods and Tissue Damage Mediated with Their Photothermal Effect

2008 ◽  
Vol 1138 ◽  
Author(s):  
Takuro Niidome ◽  
Yasuyuki Akiyama ◽  
Kohei Shimoda ◽  
Takahito Kawano ◽  
Takeshi Mori ◽  
...  
Keyword(s):  
Surface Plasmon ◽  
Gold Nanorods ◽  
Near Infrared ◽  
Tumor Therapy ◽  
Infrared Light ◽  
Photothermal Effect ◽  
Integrating Sphere ◽  
Plasmon Band ◽  
Near Infrared Light

AbstractGold nanorods have a strong surface plasmon band at the near infrared region. The absorbed light energy is then converted to heat. Since near infrared light can penetrate deeply into tissue, gold nanorods are expected to be used as a contrast agent for bioimaging using the near infrared light and photosensitizers for photothermal therapy. The surface plasmon bands of intravenously injected the gold nanorods were directly monitored from the mouse abdomen by using a spectrophotometer equipped with an integrating sphere. The absorbance at 900 nm from PEG5,000-modified gold nanorods immediately increased after injection and reached a plateau. The injection of phosphatidylcholine-modified gold nanorods also increased the absorbance at 900 nm, but the absorbance decreased single exponentially with a 1.3-min half-life. To demonstrate photothermal tumor therapy, the PEG-modified gold nanorods were directly injected into subcutaneous tumors in mice, then, near infrared laser light was irradiated to the tumor. After the treatment, significant suppression of tumor growth was observed.

scholarly journals Near-infrared light modulated photothermal effect increases vascular perfusion and enhances polymeric drug delivery

2011 ◽  
Vol 156 (2) ◽  
pp. 265-272 ◽  
Author(s):  
Marites P. Melancon ◽  
Andrew M. Elliott ◽  
Anil Shetty ◽  
Qian Huang ◽  
R. Jason Stafford ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Near Infrared ◽  
Infrared Light ◽  
Photothermal Effect ◽  
Vascular Perfusion ◽  
Near Infrared Light ◽  
Polymeric Drug Delivery ◽  
Polymeric Drug

scholarly journals Microfluidic Paper-Based Analytical Devices for Colorimetric Detection of Lactoferrin

2019 ◽  
Vol 25 (1) ◽  
pp. 47-57 ◽  
Author(s):  
Hiroko Kudo ◽  
Kento Maejima ◽  
Yuki Hiruta ◽  
Daniel Citterio
Keyword(s):  
Color Change ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Color Intensity ◽  
Colorimetric Sensing ◽  
Detection Mechanism ◽  
Fiber Network ◽  
Water Dispersible ◽  
Physical Entrapment

Lactoferrin is an abundant glycoprotein in human body fluids and is known as a biomarker for various diseases. Therefore, point-of-care testing (POCT) for lactoferrin is of interest. Microfluidic paper-based analytical devices (µPADs) have gained a lot of attention as next-generation POCT device candidates, due to their inexpensiveness, operational simplicity, and being safely disposable. This work presents a colorimetric sensing approach for quantitative lactoferrin analysis. The detection mechanism takes advantage of the high affinity of lactoferrin to ferric ions (Fe3+). Lactoferrin is able to displace an indicator from a colorimetric 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (5-Br-PADAP)-Fe3+ complex, resulting in a color change. A 5-Br-PADAP-Fe3+ complex was encapsulated into water-dispersible poly(styrene- block-vinylpyrrolidone) particles, whose physical entrapment in the cellulosic fiber network results in the immobilization of the complex to the paper matrix. The complex-encapsulating particles showed a color change response in accordance with lactoferrin concentration. Both color intensity-based paper well plates and distance readout-based µPADs are demonstrated. Color intensity-based devices allowed quantitative analysis of lactoferrin concentrations with a limit of detection of 110 µg/mL, using a smartphone and a color readout app. On the other hand, distance readout-based µPADs showed changes of the length of colored sections in accordance with lactoferrin concentration. In summary, we successfully developed both colorimetric intensity-based paper wells and distance-based µPADs for lactoferrin detection. This work demonstrates a user-friendly colorimetric analysis platform for lactoferrin without requiring lab equipment and expensive antibodies.

scholarly journals Self-Assembly Iron Oxide Nanoclusters for Photothermal-Mediated Synergistic Chemo/Chemodynamic Therapy

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Xiang Li ◽  
Zhen Wang ◽  
Mian Ma ◽  
Zhouqing Chen ◽  
Xiang-long Tang ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Self Assembly ◽  
Near Infrared ◽  
Light Irradiation ◽  
Infrared Light ◽  
Photothermal Effect ◽  
Photothermal Conversion ◽  
Assembly Method

Background and Purpose. Although chemodynamic therapy (CDT) is promising for cancer treatment, its clinical application is still limited because of unresolved issues. In this study, an efficient CDT agent for synergistic chemo/CDT therapy mediated by the photothermal effect was developed by an iron oxide self-assembly method. Methods. Superparamagnetic iron oxide nanoclusters (SPIOCs) were located within the core, which resulted in high photothermal conversion and outstanding generation of reactive oxygen species (ROS). The shell consisted of a human serum albumin- (HSA-) paclitaxel (PTX) layer, which extended the blood circulation time and ensured the effectiveness of the chemotherapy. Arg-Gly-Asp peptides (RGD) were linked to the naked cysteine moieties in HSA to promote the specific targeting of human glioma U87 cells by αvβ3 integrins. Continuous near-infrared light irradiation triggered and promoted the synergistic chemo/CDT therapy through the photothermal effect. Results. Our SPIOCs@HSA-RGD nanoplatform showed well biocompatibility and could target glioma specifically. Photothermal conversion and ROS burst were detected after continuous 808 nm light irradiation, and a significant antitumor effect was achieved. Conclusion. Experimental in vitro and in vivo evaluations showed that our photothermal-mediated chemo/CDT therapy could efficiently inhibit tumor growth and is therefore promising for cancer therapy.

scholarly journals Noninvasive Near-Infrared Light Triggers Remote Activation of Thermo-Responsive TRPV1 Channels in Neurons Based on Biodegradable/Photothermal Polymer Micelles

Nanoscale ◽  
2022 ◽  
Author(s):  
Wei-Hsu Chen ◽  
Taiki Onoe ◽  
Masao Kamimura
Keyword(s):  
Ion Channel ◽  
Near Infrared ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Cation Channel ◽  
Infrared Light ◽  
Temperature Sensitive ◽  
Polymer Micelle ◽  
Trpv1 Channels ◽  
Transient Receptor

In this study, we developed a novel biodegradable/photothermal polymer micelle-based remote-activation method for a temperature-sensitive ion channel, namely transient receptor potential cation channel subfamily V member 1 (TRPV1). Biodegradable/photothermal polymer...

Near-Infrared Light Enhanced Peroxidase-Like Activity of PEGylated Palladium Nanozyme for Highly Efficient Biofilm Eradication

2021 ◽  
Vol 17 (6) ◽  
pp. 1131-1147
Author(s):  
Sijin Xiang ◽  
Zhongxiong Fan ◽  
Duo Sun ◽  
Tianbao Zhu ◽  
Jiang Ming ◽  
...  
Keyword(s):  
Infection Control ◽  
Near Infrared ◽  
Antimicrobial Agents ◽  
Infrared Light ◽  
Photothermal Effect ◽  
Highly Efficient ◽  
Nir Light ◽  
Biofilm Infection

The overall eradication of biofilm-mode growing bacteria holds significant key to the answer of a series of infection-related health problems. However, the extracellular matrix of bacteria biofilms disables the traditional antimicrobials and, more unfortunately, hampers the development of the anti-infectious alternatives. Therefore, highly effective antimicrobial agents are an urgent need for biofilm-infection control. Herein, a PEGylated palladium nanozyme (Pd-PEG) with peroxidase (POD)-like activity for highly efficient biofilm infection control is reported. Pd-PEG also shows the intrinsic photothermal effect as well as near-infrared (NIR) light-enhanced POD-like activity in the acidic environment, thereby massively destroying the biofilm matrix and killing the adhering bacteria. Importantly, the antimicrobial mechanism of the synergistic treatment based on Pd-PEG+H2O2+NIR combination was disclosed. In vitro and in vivo results illustrated the designed Pd-PEG+H2O2 +NIR treatment reagent possessed outstanding antibacterial and biofilms elimination effects with negligible biotoxicity. This work hopefully facilitates the development of metal-based nanozymes in biofilm related infectious diseases.

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