scholarly journals Molecular Detection of Cryptosporidium spp. and Enterocytozoon bieneusi Infection in Wild Rodents From Six Provinces in China

2021 ◽  
Vol 11 ◽  
Author(s):  
Hong-Bo Ni ◽  
Yu-Zhe Sun ◽  
Si-Yuan Qin ◽  
Yan-Chun Wang ◽  
Quan Zhao ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Pcr Amplification ◽  
Enterocytozoon Bieneusi ◽  
Rrna Gene ◽  
Wild Rodents ◽  
Potential Source ◽  
Its Gene ◽  
Human Infections ◽  
First Time ◽  
Genotype Ii

Enterocytozoon (E.) bieneusi and Cryptosporidium spp. are the most important zoonotic enteric pathogens associated with diarrheal diseases in animals and humans. However, it is still not known whether E. bieneusi and Cryptosporidium spp. are carried by wild rodents in Shanxi, Guangxi, Zhejiang, Shandong, and Inner Mongolia, China. In the present study, a total of 536 feces samples were collected from Rattus (R.) norvegicus, Mus musculus, Spermophilus (S.) dauricus, and Lasiopodomys brandti in six provinces of China, and were detected by PCR amplification of the SSU rRNA gene of Cryptosporidium spp. and ITS gene of E. bieneusi from June 2017 to November 2020. Among 536 wild rodents, 62 (11.6%) and 18 (3.4%) samples were detected as E. bieneusi- and Cryptosporidium spp.-positive, respectively. Differential prevalence rates of E. bieneusi and Cryptosporidium spp. were found in different regions. E. bieneusi was more prevalent in R. norvegicus, whereas Cryptosporidium spp. was more frequently identified in S. dauricus. Sequence analysis indicated that three known Cryptosporidium species/genotypes (Cryptosporidium viatorum, Cryptosporidium felis, and Cryptosporidium sp. rat genotype II/III) and two uncertain Cryptosporidium species (Cryptosporidium sp. novel1 and Cryptosporidium sp. novel2) were present in the investigated wild rodents. Meanwhile, 5 known E. bieneusi genotypes (XJP-II, EbpC, EbpA, D, and NCF7) and 11 novel E. bieneusi genotypes (ZJR1 to ZJR7, GXM1, HLJC1, HLJC2, and SDR1) were also observed. This is the first report for existence of E. bieneusi and Cryptosporidium spp. in wild rodents in Shanxi, Guangxi, Zhejiang, and Shandong, China. The present study also demonstrated the existence of E. bieneusi and Cryptosporidium spp. in S. dauricus worldwide for the first time. This study not only provided the basic data for the distribution of E. bieneusi and Cryptosporidium genotypes/species, but also expanded the host range of the two parasites. Moreover, the zoonotic E. bieneusi and Cryptosporidium species/genotypes were identified in the present study, suggesting wild rodents are a potential source of human infections.

Identification of Cryptosporidium species, Enterocytozoon bieneusi genotypes, and Giardia duodenalis assemblages in birds in Henan, China

2019 ◽  
Author(s):  
Haiju Dong ◽  
Ru Cheng ◽  
Xinmiao Li ◽  
Junqiang Li ◽  
Yuancai Chen ◽  
...  
Keyword(s):  
Migratory Birds ◽  
Giardia Duodenalis ◽  
Pcr Amplification ◽  
Small Subunit ◽  
Enterocytozoon Bieneusi ◽  
Rrna Gene ◽  
Fecal Samples ◽  
Zoonotic Pathogens ◽  
Its Gene ◽  
Turtle Dove

Abstract BackgroundDomesticated, wild, and migratory birds have been known to transmit diseases such as diarrhea in humans and other animals, but studies specifically on the zoonotic pathogens Cryptosporidium spp., Enterocytozoon bieneusi, and Giardia duodenalis in birds in Henan Province, China are lacking. Hence, this study sought to characterize the prevalence of these pathogens, and to identify the different species of Cryptosporidium and their phylogenetic relationships, the genotypes of E. bieneusi, and the assemblages of G. duodenalis, in birds in the province. MethodsFresh fecal samples were collected from birds in parks and pet shops in Henan, China and were screened for the presence of the pathogens using nest-PCR amplification of the small subunit ribosomal RNA (SSU rRNA) gene and the internal transcribed spacer (ITS) gene. ResultsA total of 1,005 fecal samples were collected from 32 species of birds. 21 fecal samples (2.09%) were found positive for Cryptosporidium spp., 45 (4.48%) for E. bieneusi, and 33 (3.28%) for G. duodenalis. This study identified five Cryptosporidium species: C. baileyi (10 out of 21 fecal samples, 47.62%) in crested myna (Acridotheres cristatellus), Java sparrow (Lonchura oryzivora), Chinese hwamei (Garrulax canorus), common quail (Coturnix coturnix), and Chinese grosbeak (Eophona migratoria); C. galli (5/21, 23.81%) in Chinese blackbird (Turdus mandarinus), zebra finch (Taeniopygia guttata), and white-eyes (Zosterops sp.); C. andersoni (1/21, 4.76%) in a white-eye for the first time; C. meleagridis (4/21, 19.05%) in parrots and crested myna; and C. parvum (1/21, 4.76%) in a pigeon. Two E. bieneusi genotypes: Peru6 and PtEb I were found in pigeons and European turtle dove (Streptopelia turtur). The G. duodenalis assemblage E was detected in parrots, common hill myna, crested myna, Java sparrow, white-eyes, black-throated laughingthrush, and other birds. ConclusionsOur findings indicate that the aforementioned species of birds in Henan, China could be a source of zoonotic pathogens, such as C. meleagridis, C. andersoni, C. parvum, E. bieneusi genotype Peru6, and G. duodenalis assemblage E, that cause diseases in humans.

Diversity of nodular bacteria ofScorpiurus muricatusin western Algeria and their impact on plant growth

2017 ◽  
Vol 63 (5) ◽  
pp. 450-463 ◽  
Author(s):  
Zoulikha Bouchiba ◽  
Zineb Faiza Boukhatem ◽  
Zohra Ighilhariz ◽  
Nouria Derkaoui ◽  
Benaissa Kerdouh ◽  
...  
Keyword(s):  
Plant Growth ◽  
Rhizobium Leguminosarum ◽  
Root Nodules ◽  
Pcr Amplification ◽  
Nucleotide Sequencing ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Single Strain ◽  
Western Algeria ◽  
First Time

A total of 51 bacterial strains were isolated from root nodules of Scorpiurus muricatus sampled from 6 regions of western Algeria. Strain diversity was assessed by rep-PCR amplification fingerprinting, which grouped the isolates into 28 different clusters. Partial nucleotide sequencing of the 16S rRNA gene and BLAST analysis revealed that root nodules of S. muricatus were colonized by different species close to Rhizobium vignae, Rhizobium radiobacter, Rhizobium leguminosarum, Phyllobacterium ifriqiyense, Phyllobacterium endophyticum, Starkeya sp., and Pseudomonas sp. However, none of these strains was able to form nodules on its host plant; even nodC was present in a single strain (SMT8a). The inoculation test showed a great improvement in the growth of inoculated plants compared with noninoculated control plants. A significant amount of indole acetic acid was produced by some strains, but only 2 strains could solubilize phosphate. In this report we described for the first time the diversity of bacteria isolated from root nodules of S. muricatus growing in different regions in western Algeria and demonstrated their potential use in promoting plant growth.

scholarly journals Host-adaptation of the rare Enterocytozoon bieneusi genotype CHN4 in Myocastor coypus (Rodentia: Echimyidae) in China

2020 ◽  
Author(s):  
Fuchang Yu ◽  
Yangwenna Cao ◽  
Haiyan Wang ◽  
Qiang Liu ◽  
Aiyun Zhao ◽  
...  
Keyword(s):  
Infection Rate ◽  
Ribosomal Rna ◽  
Pcr Amplification ◽  
Its Region ◽  
Host Adaptation ◽  
Enterocytozoon Bieneusi ◽  
Rrna Gene ◽  
Myocastor Coypus ◽  
Gastrointestinal Pathogen ◽  
Infection Source

Abstract Background: Enterocytozoon bieneusi is a zoonotic gastrointestinal pathogen and can infect both humans and animals. The coypu (Myocastor coypus) is a semi-aquatic rodent, in which few E. bieneusi infections have been reported and the distribution of genotypes and zoonotic potential remains unknown.Methods: A total of 308 fresh fecal samples were collected from seven coypu farms in China to determine the infection rate and the distribution of genotypes of E. bieneusi from coypus using nested-PCR amplification of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene.Results: Enterocytozoon bieneusi was detected with an infection rate of 41.2% (n = 127). Four genotypes were identified, including three known genotypes (CHN4 (n = 111), EbpC (n = 8) and EbpA (n = 7)) and a novel genotype named CNCP1 (n = 1). Conclusions: The rare genotype CHN4 was the most common genotype in the present study, and the transmission dynamics of E. bieneusi in coypus were different from other rodents. To the best of our knowledge, this is the first report of E. bieneusi infections in coypus in China. Our study reveals that E. bieneusi in coypus may be a potential infection source to humans.

scholarly journals Molecular Detection and Characterization of Goat Isolate ofTaenia hydatigenain Turkey

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Armagan Erdem Utuk ◽  
Fatma Cigdem Piskin
Keyword(s):  
Ribosomal Rna ◽  
Molecular Detection ◽  
Sequence Data ◽  
Pcr Amplification ◽  
Small Subunit ◽  
Molecular Tools ◽  
Taenia Hydatigena ◽  
Nucleotide Sequence Data ◽  
First Time

The aim of this study was to provide molecular detection and characterization of the goat isolate ofTaenia hydatigenafrom Ankara province of Turkey. For this purpose, PCR amplification of small subunit ribosomal RNA (rrnS) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) genes were performed in a one-month-old dead goat. According to rrnS-PCR results, parasites were identified asTaeniaspp., and partial sequence of mt-CO1 gene was corresponding toT. hydatigena. At the end of the study, we concluded that molecular tools can be used to define species of parasites in cases where the key morphologic features cannot be detected. Nucleotide sequence data of Turkish goat isolate ofT. hydatigenawas submitted to GenBank for other researchers interested in this subject. By this study, molecular detection and characterization ofT. hydatigenawas done for the first time in Turkey.

scholarly journals Molecular Survey and Genetic Diversity of Hemoplasmas in Rodents from Chile

2020 ◽  
Vol 8 (10) ◽  
pp. 1493
Author(s):  
Amir Salvador Alabí ◽  
Gustavo Monti ◽  
Carola Otth ◽  
Paulina Sepulveda-García ◽  
Melissa Sánchez-Hidalgo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
16S Rrna ◽  
Rrna Gene ◽  
Polymorphism Analysis ◽  
Wild Rodents ◽  
High Identity ◽  
Low Diversity ◽  
Hemotrophic Mycoplasma ◽  
First Time ◽  
The 16S Rrna Gene

Even though hemotrophic mycoplasma (hemoplasma) infections are well documented in a wide variety of hosts worldwide, there is a gap in the knowledge aobut hemoplasmas in rodents. This study aimed to molecularly survey and investigate the genetic diversity of hemoplasmas in rodents from Chile. Synanthropic and wild rodents (n = 74) were captured in the southern province of Valdivia (Corral, Valdivia, Riñihue, and Reumén localities). Spleen samples were submitted to a conventional PCR for hemotrophic Mycoplasma spp. targeting the 16S rRNA gene (800 bp), followed by sequencing, phylogenetic, and genetic diversity analyses. The overall occurrence of hemotrophic mycoplasmas in rodents from Valdivia was 24.5% (18/74) [95% CI (14.5; 34.1)]. Hemoplasmas were detected in Mus musculus (1/4), Rattus norvegicus (1/16), Abrothrix longipilis (7/13), A. olivaceo (6/8), and Oligoryzomys longicaudatus (3/10). The nucleotide polymorphism analysis of the targeted 16S rRNA region showed low diversity, with two genotypes and a high identity to the variants detected in wild rodents from Brazil. Hemoplasmas are described for the first time in rodents from Chile with a moderate occurrence and low 16S rDNA genetic diversity within the sampled rodent population. The detected hemoplasma genotypes were specific to rodents and were not shared with other mammals.

scholarly journals Molecular Evidence of Bartonella spp. in Rodents: A Study in Pianosa Island, Italy

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2070
Author(s):  
Sara Divari ◽  
Paola Pregel ◽  
Stefania Zanet ◽  
Ezio Ferroglio ◽  
Francesca Giannini ◽  
...  
Keyword(s):  
Citrate Synthase ◽  
Gene Segment ◽  
Pcr Amplification ◽  
Amplicon Sequencing ◽  
23S Rrna ◽  
Molecular Evidence ◽  
Rrna Gene ◽  
Wild Rodents ◽  
Conventional Pcr ◽  
23S Rrna Gene

Wild rodents are reservoirs of several Bartonella species that cause human bartonellosis. The aim of this study was to assess the presence of Bartonella spp. DNA in wild rodents in Pianosa island, Italy. Rats (Rattus spp.; n = 15) and field mice (Apodemus spp.; n = 16) were captured and spleen DNA tested for the presence of Bartonella spp. by means of an initial screening using a qPCR amplifying a short segment of the 16S-23S rRNA gene intergenic transcribed spacer region (ITS, ~200 bp) followed by conventional PCR amplification of a longer ITS fragment (~600 bp) and of a citrate synthase (gltA, ~340 bp) gene segment. A total of 25 spleen DNA samples obtained from 31 rodent carcasses (81%) yielded positive qPCR results. Bartonella genus was confirmed by amplicon sequencing. By conventional PCR, eight out of 25 samples (32%) yielded bands on gels consistent with ITS segment, and 6/25 (24%) yielded bands consistent with the gltA locus. Amplicon sequencing identified B. henselae and B. coopersplainsensis in 1/25 (4%), and 4/25 (16%) samples, respectively. Moreover, 5/25 (20%) of Bartonella spp. positive samples showed gltA sequences with about 97% identity to B. grahamii. These results provide support to recently published observations suggesting that B. henselae circulates in wild rodent populations.

scholarly journals Prevalence and Subtypes of Blastocystis in Alpacas, Vicugna pacos in Shanxi Province, China

2020 ◽  
Vol 58 (2) ◽  
pp. 181-184 ◽  
Author(s):  
Ye-Ting Ma ◽  
Qing Liu ◽  
Shi-Chen Xie ◽  
Xiao-Dong Li ◽  
Yuan-Yuan Ma ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Pcr Amplification ◽  
Northern China ◽  
Small Subunit ◽  
Human Infection ◽  
Shanxi Province ◽  
Rrna Gene ◽  
Small Subunit Rrna ◽  
Potential Source ◽  
And Gender

<i>Blastocystis</i>, an enteric protist, has been reported to be an important cause of protozoal gastrointestinal manifestations in humans and animals worldwide. Animals harboring certain <i>Blastocystis</i> subtypes (STs) may serve as a potential source of human infection. However, information about the prevalence and genetic diversity of <i>Blastocystis</i> in alpacas is limited. In the present study, a total of 366 fecal samples from alpacas in Shanxi Province, northern China, were examined for <i>Blastocystis</i> by PCR amplification of the small subunit rRNA gene, followed by sequencing and phylogenetic analysis. The prevalence of <i>Blastocystis</i> in alpacas was 23.8%, and gender difference in the prevalence of <i>Blastocystis</i>was observed. The most predominant <i>Blastocystis</i> ST was ST10, followed by ST14 and ST5. The detection of ST5, a potentially zoonotic genotype, indicates that alpacas harboring ST5 could be a potential source of human infection with <i>Blastocystis</i>. These data provide new insight into the prevalence and genetic diversity of <i>Blastocystis</i> in alpacas.

scholarly journals Molecular Characterization of Microsporidia Indicates that Wild Mammals Harbor Host-Adapted Enterocytozoon spp. as well as Human-Pathogenic Enterocytozoon bieneusi

2003 ◽  
Vol 69 (8) ◽  
pp. 4495-4501 ◽  
Author(s):  
Irshad M. Sulaiman ◽  
Ronald Fayer ◽  
Altaf A. Lal ◽  
James M. Trout ◽  
Frank W. Schaefer ◽  
...  
Keyword(s):  
Enterocytozoon Bieneusi ◽  
Rrna Gene ◽  
Internal Transcribed Spacer Region ◽  
Wild Mammals ◽  
Potential Source ◽  
Public Health Significance ◽  
Multiple Alignments ◽  
Health Significance ◽  
Multiple Species

ABSTRACT Over 13 months, 465 beavers, foxes, muskrats, otters, and raccoons were trapped in four counties in eastern Maryland and examined by molecular methods for microsporidia. A two-step nested PCR protocol was developed to amplify a 392-bp fragment of the internal transcribed spacer region of the rRNA gene of Enterocytozoon spp., with the use of primers complementary to the conserved regions of published nucleotide sequences. Fifty-nine PCR-positive samples were sequenced. Multiple alignments of these sequences identified 17 genotypes of Enterocytozoon spp. (WL1 to WL17); of these, 15 have not been reported before. Most of the genotypes were found in multiple species of wildlife and belonged to a major group consisting of all the previously described Enterocytozoon bieneusi genotypes from human and domestic animals. Some of the isolates from muskrats and raccoons formed two distinct groups. Results of this study indicate that fur-bearing mammals, especially those closely associated with surface water, can be a potential source of human-pathogenic E. bieneusi. However, there are also host-adapted Enterocytozoon genotypes in wildlife, which may represent species different from E. bieneusi and have no apparent public health significance. This is the first report of E. bieneusi in wildlife.

scholarly journals Host-adaptation of the rare Enterocytozoon bieneusi genotype CHN4 in Myocastor coypus (Rodentia: Echimyidae) in China

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Fuchang Yu ◽  
Yangwenna Cao ◽  
Haiyan Wang ◽  
Qiang Liu ◽  
Aiyun Zhao ◽  
...  
Keyword(s):  
Infection Rate ◽  
Ribosomal Rna ◽  
Pcr Amplification ◽  
Its Region ◽  
Host Adaptation ◽  
Enterocytozoon Bieneusi ◽  
Rrna Gene ◽  
Myocastor Coypus ◽  
Gastrointestinal Pathogen ◽  
Infection Source

Abstract Background Enterocytozoon bieneusi is a zoonotic gastrointestinal pathogen and can infect both humans and animals. The coypu (Myocastor coypus) is a semi-aquatic rodent, in which few E. bieneusi infections have been reported and the distribution of genotypes and zoonotic potential remains unknown. Methods A total of 308 fresh fecal samples were collected from seven coypu farms in China to determine the infection rate and the distribution of genotypes of E. bieneusi from coypus using nested-PCR amplification of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene. Results Enterocytozoon bieneusi was detected with an infection rate of 41.2% (n = 127). Four genotypes were identified, including three known genotypes (CHN4 (n = 111), EbpC (n = 8) and EbpA (n = 7)) and a novel genotype named CNCP1 (n = 1). Conclusions The rare genotype CHN4 was the most common genotype in the present study, and the transmission dynamics of E. bieneusi in coypus were different from other rodents. To the best of our knowledge, this is the first report of E. bieneusi infections in coypus in China. Our study reveals that E. bieneusi in coypus may be a potential infection source to humans.

scholarly journals Prevalence and genotyping identification of Cryptosporidium in adult ruminants in central Iran

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Zohre Firoozi ◽  
Alireza Sazmand ◽  
Alireza Zahedi ◽  
Akram Astani ◽  
Ali Fattahi-Bafghi ◽  
...  
Keyword(s):  
Pcr Amplification ◽  
Central Iran ◽  
Human Consumption ◽  
Human Populations ◽  
Rrna Gene ◽  
Apicomplexan Parasites ◽  
Faecal Samples ◽  
Wide Range ◽  
Life Threatening ◽  
First Time

Abstract Background Apicomplexan parasites of the genus Cryptosporidium infect a wide range of animal species as well as humans. Cryptosporidium spp. can cause life threatening diarrhea especially in young animals, children, immunocompromised patients and malnourished individuals. Asymptomatic cryptosporidial infections in animals can also occur, making these animals potential reservoirs of infection. Methods In the present study, a molecular survey of Cryptosporidium spp. in ruminants that were slaughtered for human consumption in Yazd Province, located in central Iran was conducted. Faeces were collected per-rectum from 484 animals including 192 cattle, 192 sheep and 100 goats. DNA was extracted from all samples and screened for Cryptosporidium by PCR amplification of the 18S rRNA gene. Positives were Sanger sequenced and further subtyped by sequence analysis of the 60 kDa glycoprotein (gp60) locus. Results In total, Cryptosporidium spp. were detected in 22 animals: C. andersoni and C. bovis in seven and two cattle faecal samples, respectively, C. ubiquitum in five sheep, and C. xiaoi in six sheep and two goat samples, respectively. To our knowledge, this study provides for the first time, molecular information concerning Cryptosporidium species infecting goats in Iran, and is also the first report of C. ubiquitum and C. xiaoi from ruminants in Iran. Conclusion The presence of potentially zoonotic species of Cryptosporidium in ruminants in this region may suggest that livestock could potentially contribute to human cryptosporidiosis, in particular among farmers and slaughterhouse workers, in the area. Further molecular studies on local human populations are required to more accurately understand the epidemiology and transmission dynamics of Cryptosporidium spp. in this region.

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