Corrigendum: Effects of Gender of Reciprocal Chromosomal Translocation on Blastocyst Formation and Pregnancy Outcome in Preimplantation Genetic Testing

ObjectiveTo determine the effect of gender of reciprocal chromosomal translocation on blastocyst formation and pregnancy outcome in preimplantation genetic testing, including different parental ages.MethodsThis was a retrospective cohort study that enrolled 1034 couples undergoing preimplantation genetic testing-structural rearrangement on account of a carrier of reciprocal chromosomal translocation from the Reproductive Medicine Center of the First Affiliated Hospital of Zhengzhou University from January 2015 to December 2019. Group A represented 528 couples in which the man was the carrier of reciprocal translocation and group B represented 506 couples in which the woman was the carrier of reciprocal translocation. All patients were divided into two groups according to their age: female age<35 and female age≥35. Furthermore, the differences in blastocyst condition and pregnancy outcome between male and female carriers in each group were further explored according to their father’s age.ResultsThe blastocyst formation rate of group A (55.3%) is higher than that of group B (50%) and the results were statistically significant (P<0.05). The blastocyst formation rate of group A is higher than that of group B, no matter in young maternal age or in advanced maternal age (P<0.05). The blastocyst formation rate in maternal age<35y and paternal age<30y in group A(57.1%) is higher than that of Group B(50%); Similarly, the blastocyst formation rate in maternal age≥35 and paternal age≥38y(66.7%) is higher than that of Group B(33.3%)(all P<0.05). There was no difference in fertilization rate, aeuploidy rate, clinical pregnancy rate, miscarriage rate and live birth rate between Group A and Group B.ConclusionWhen the carrier of reciprocal translocation is male, the blastocyst formation rate is higher than that of female carrier. While there is no significant difference between the two in terms of fertilization rate, aeuploidy rate, clinical pregnancy rate, miscarriage rate and live birth rate.

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Morphokinetic parameters in chromosomal translocation carriers undergoing preimplantation genetic testing

Reproductive BioMedicine Online ◽

10.1016/j.rbmo.2018.11.006 ◽

2019 ◽

Vol 38 (2) ◽

pp. 177-183

Author(s):

Jenna Lammers ◽

Arnaud Reignier ◽

Carole Splingart ◽

Kamran Moradkhani ◽

Paul Barrière ◽

...

Keyword(s):

Genetic Testing ◽

Chromosomal Translocation ◽

Preimplantation Genetic Testing ◽

Morphokinetic Parameters

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Preimplantation genetic testing for aneuploidy: are we examining the correct outcomes?

Human Reproduction ◽

10.1093/humrep/deaa224 ◽

2020 ◽

Vol 35 (11) ◽

pp. 2408-2412

Author(s):

James M Kemper ◽

Rui Wang ◽

Daniel L Rolnik ◽

Ben W Mol

Keyword(s):

Genetic Testing ◽

Randomized Controlled Trials ◽

Pregnancy Outcome ◽

Controlled Trials ◽

Preimplantation Genetic Testing ◽

Live Birth Rates ◽

Randomized Controlled ◽

Cumulative Live Birth ◽

Focus On Results ◽

Genetic Assessment

ABSTRACT Questions continue to be raised regarding the benefit of genetic assessment of embryos prior to transfer in IVF, specifically with regards to preimplantation genetic testing for aneuploidy (PGT-A). To evaluate and quantify these concerns, we appraised the most recent (2012–2019) randomized controlled trials on the topic. Only two of these six studies listed cumulative live birth rates per started cycle, with both eliciting a statistically non-significant result. This article describes the concern that a focus on results from the first embryo transfer compared to cumulative outcomes falsely construes PGT-A as having superior outcomes, whilst its true benefit is not confirmed, and it cannot actually improve the true pregnancy outcome of an embryo pool.

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The Influence of Chromosomal Polymorphism on Embryo Development and Embryonic Molecular Karyotype in Preimplantation Genetic Testing for Chromosomal Translocation

Frontiers in Physiology ◽

10.3389/fphys.2020.543188 ◽

2020 ◽

Vol 11 ◽

Author(s):

Gang Li ◽

Weiyi Shi ◽

Wenbin Niu ◽

Jiawei Xu ◽

Yihong Guo ◽

...

Keyword(s):

Genetic Testing ◽

Statistical Difference ◽

Chromosomal Translocation ◽

Fertilization Rate ◽

Cleavage Rate ◽

High Quality ◽

Preimplantation Genetic Testing ◽

Abnormal Embryo ◽

Molecular Karyotype ◽

Blastocyst Rate

Traditionally, chromosomal polymorphisms (CPMs) are normal genetic variants in individuals with no phenotypic variations. However, some studies have shown that CPM is related to reproductive diseases. We explored the influence of CPM on embryonic development and molecular karyotype in chromosomal translocation (CT) patients undergoing preimplantation genetic testing (PGT) between February 2013 and May 2019. Twenty-six cases with CPM and 56 controls with normal chromosomes were included. Furthermore, a 1:4 match pair analysis by female age included 39 cases with CTCPM and 185 controls with CT. There was no statistical difference in fertilization rate (78.48% vs. 78.33%), cleavage rate on Day 3 (90.32% vs. 89.16%), blastocyst rate (60.00% vs. 60.80%), and the high-quality blastocyst rate (36.31% vs. 35.22%) between CPM and normal chromosomes. The high-quality blastocyst rate of CTCPM was significantly lower than that for CT (26.78% vs. 38.89%). Moreover, there was no statistical difference in fertilization rate (70.65% vs. 70.37%), cleavage rate on Day 3 (88.67% vs. 89.53%), and blastocyst rate (48.48% vs. 53.17%) between CTCPM and CT. In addition, one CTCPM spouse had a lower high-quality blastocyst rate, especially of males with CTCPM. Abnormal embryo rates of CTCPM were significantly higher than those for CT (78.64% vs. 68.93%). Abnormal embryo rates were higher in both CTCPM and CPM paternal carriers with CT partners, respectively. For CT, CTCPM may have an impact on the high-quality blastocyst rate and embryonic molecular karyotype, especially in male patients. Patients with CTCPM are relatively rare, but this population would benefit from being explored using a larger sample size.

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The Influence of Sex on Embryo Development and Pregnancy Outcome in Preimplantation Genetic Testing for Chromosomal Translocation

10.21203/rs.3.rs-868456/v1 ◽

2021 ◽

Author(s):

Yile Zhang ◽

Hui Song ◽

Jing-di Li ◽

Zhi-qin Bu ◽

Fang Wang ◽

...

Keyword(s):

Pregnancy Outcome ◽

Reciprocal Translocation ◽

Robertsonian Translocation ◽

Formation Rate ◽

Parental Age ◽

Blastocyst Formation ◽

Preimplantation Genetic Testing ◽

Male Carriers ◽

In Pregnancy ◽

Female Carriers

Abstract Background To investigate the embryonic development and clinical pregnancy outcome of reciprocal translocation carriers and Robertsonian translocation carriers with different sex in preimplantation genetic testing (PGT). Methods A retrospective analysis of 1369 cycles of preimplantation genetic testing for structural rearrangements (PGT-SR) was performed in the Reproductive Medicine Center of the First Affiliated Hospital of Zhengzhou University from 2015 to 2019. All the patients were divided into reciprocal translocation and Robertsonian translocation according to the type of chromosomal translocation and divided into female carriers and male carriers according to the sex of the carriers. SPSS21.0 was used for data statistics and P < 0.05 indicated that the difference was statistically significant. Results The fertilization rate of female carriers(81.5%) with chromosomal structural abnormalities (including reciprocal translocation and Robertsonian translocation) was higher than that of male carriers(80.0%)(P=0.032), and the blastocyst formation rate of female carriers(50.0%) was lower than that of male carriers(54.8%)(P=0.016) in the same parental age. But there was no statistical difference in cleavage rate, high quality embryo rate, normal rate of biopsy, clinical pregnancy rate, abortion rate and live birth rate between female and male carriers. In the reciprocal translocation group, the blastocyst formation rate of male carriers (54.8%) was higher than that of female carriers (50.0%) (P=0.022) with the same parental age and there was no difference in pregnancy outcome. In the Robertsonian translocation group, the fertilization rate of male carriers (75.0%) was lower than that of female carriers (81.8%) (P=0.005) and the normal rate of biopsy (33.3%) was higher than that of female carriers (25.0%) (P=0.022) with the same parental age and there was no difference in pregnancy outcome. Conclusions In reciprocal translocation, male carriers have a higher rate of blastocyst formation rate than female carriers. In Robertsonianian translocation, male carriers have a higher noamal rate of biopsy than female carriers. However, there was no significant difference in pregnancy outcome between male carriers and female carriers with abnormal chromosome structure.

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Preimplantation genetic testing for aneuploidy and subsequent blastocyst transfer improves pregnancy outcome in advanced maternal age patients

The Onco Fertility Journal ◽

10.4103/tofj.tofj_14_19 ◽

2019 ◽

Vol 2 (2) ◽

pp. 74

Author(s):

Surleen Kaur ◽

Beena Rawat

Keyword(s):

Genetic Testing ◽

Pregnancy Outcome ◽

Maternal Age ◽

Advanced Maternal Age ◽

Blastocyst Transfer ◽

Preimplantation Genetic Testing

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The Pregnancy Outcome of Mosaic Embryo Transfer: A Prospective Multicenter Study and Meta-Analysis

Genes ◽

10.3390/genes11090973 ◽

2020 ◽

Vol 11 (9) ◽

pp. 973

Author(s):

Ying Xin Zhang ◽

Jang Jih Chen ◽

Sunanta Nabu ◽

Queenie Sum Yee Yeung ◽

Ying Li ◽

...

Keyword(s):

Genetic Testing ◽

Pregnancy Outcome ◽

Live Birth ◽

Birth Rate ◽

Meta Analysis ◽

Live Birth Rate ◽

Miscarriage Rate ◽

Prenatal Genetic Testing ◽

Preimplantation Genetic Testing ◽

Mosaic Embryo

Chromosomal mosaicism is at high occurrence in early developmental-stage embryos, but much lower in those at prenatal stage. Recent studies provided evidence on the viability of mosaic embryos by reporting pregnancy outcomes. Expanded research is warranted to evaluate its clinical significance. This is a multi-center prospective cohort study on 137 mosaic, 476 euploid and 835 non-preimplantation genetic testing (non-PGT) embryos from three in vitro fertilization (IVF) providers of three countries in Asia, applying the same preimplantation genetic testing for aneuploidies (PGT-A) reporting criteria. Mosaic embryo transfers (METs) resulted in a significantly lower clinical pregnancy rate (40.1% versus 59.0% versus 48.4%), lower ongoing/live birth rate (27.1% versus 47.0% versus 35.1%) and higher miscarriage rate (33.3% versus 20.5% versus 27.4%) than euploid and non-PGT transfers, respectively. Pregnancy losses after METs were different between embryos carrying numerical and segmental chromosomal abnormalities (p = 0.04). Our meta-analysis concluded that METs gave rise to pregnancies but were associated with a reduced ongoing/live birth rate and a higher miscarriage rate. All 37 MET live births were confirmed viable, among which 8 completed prenatal genetic testing with normal results. Longitudinal investigation on one MET pregnancy evidenced the aneuploidy depletion hypothesis. This is the first multi-center prospective study reporting a full MET pregnancy outcome with complementary information from prenatal genetic testing as compared to euploid and non-PGT cohorts.

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