scholarly journals Identifying Biomarkers to Predict the Prognosis of Biliary Atresia by Weighted Gene Co-Expression Network Analysis

2021 ◽  
Vol 12 ◽  
Author(s):  
Meng Kong ◽  
Bo Xiang
Keyword(s):  
Prognostic Factor ◽  
Network Analysis ◽  
Biliary Atresia ◽  
Normal Liver ◽  
Control Group ◽  
Bile Acid Metabolism ◽  
Signal Pathways ◽  
Native Liver ◽  
Native Liver Survival ◽  
Liver Tissues

The prognosis of children with biliary atresia (BA) after Kasai operation remains difficult to predict, and liver fibrosis is closely related to the prognosis of children with BA. We aimed to find biomarkers for native liver survival (NLS) prediction by weighted gene co-expression network analysis (WGCNA). The biological processes and signal pathways that biomarkers involved in were further analyzed by bioinformatics. Quantitative Real-time PCR, Western blot and immunohistochemistry was performed to detect biomarkers expression. The relationship of biomarkers with clinicopathological characteristics of BA was also investigated. LECT2 was overexpressed or knockdown in LX-2 cells, and the expression of fibrogenic genes such as a-SMA and COL1A1 was quantified. We found that LECT2 mRNA expression was higher in BA liver tissues compared with normal liver tissues. Bioinformatics showed that LECT2 mainly played a fibrosis-promoting role in the development in BA by regulating bile acid metabolism and promoting inflammatory response. LECT2 immunohistochemistry scores of BA children were higher than control group (p = 0.001). Survival analysis revealed that LECT2 high expression is an unfavorable prognostic factor for native liver survival in BA patients. Additionally, the high LECT2 expression was an independent prognostic factor affecting native liver survival (HR 3.702, 95%CI:2.085–6.575, p = 0.001). LECT2 modulates TGF-β mediated a-SMA and COL1A1 expression in LX-2 cells. siRNA-LECT2 inhibits the expression of a-SMA and COL1A1 in LX-2 cells. Overexpression of LECT2 resulted in an increase in a-SMA and COL1A1 expression. Knockdown of LECT2 inhibits the proliferation and increase apoptosis in activated LX-2 cells. LECT2 may act as a new prognostic biomarker for native liver survival in BA patients.

scholarly journals Quantitative Liver Fibrosis Using Collagen Hybridizing Peptide to Predict Native Liver Survival in Biliary Atresia

2020 ◽  
Vol 70 (1) ◽  
pp. 87-92
Author(s):  
Catalina Jaramillo ◽  
Stephen L. Guthery ◽  
Amy Lowichik ◽  
Gregory Stoddard ◽  
Taegun Kim ◽  
...  
Keyword(s):  
Liver Fibrosis ◽  
Biliary Atresia ◽  
Native Liver ◽  
Native Liver Survival

scholarly journals The prognosis of type III biliary atresia with a hilar cyst on ultrasonography might not be better than that without

2019 ◽  
Author(s):  
Quan-yuan Shan ◽  
Bao-xian Liu ◽  
Zhi-hai Zhong ◽  
Hua-dong Chen ◽  
Yu Guo ◽  
...  
Keyword(s):  
Survival Rate ◽  
Biliary Atresia ◽  
Survival Curves ◽  
Type Iii ◽  
Kaplan Meier ◽  
Significant Difference ◽  
Native Liver ◽  
Eventual Outcome ◽  
Native Liver Survival ◽  
Better Than

Abstract Background Biliary atresia (BA) with a hilar cyst is an uncommon variant of BA that may be misunderstood to have a relatively favorable prognosis. Methods A single-center retrospective review of patients that matched BA with a hilar cyst (n=27) with BA without a cyst (n=27) over a 5-years period was done. All patients were diagnosed as type III BA by histologic examination and cholangiograms. Results There were no significant intergroup differences between baseline characteristics and outcomes after Kasai portoenterostomy surgery in two groups. The Kaplan–Meier survival curves showed no significant difference in cumulative native liver survival rate of the two groups (p = 0.584). Of the 27 BA patients with a hilar cyst, 8 were showed the cyst communicated with the gallbladder on cholangiograms, and Kaplan-Meier survival curves indicated that the cumulative native liver survival rate was significantly shorter (P=0.045) in them than those who the cyst was not communicated with the gallbladder. Conclusions Type III BA with a hilar cyst had no better prognosis compared with Type III BA without a cyst. A cyst communicated with the gallbladder may be associated with a poor eventual outcome for Type III BA with a hilar cyst.

scholarly journals Bilirubin level 1 week after hepatoportoenterostomy predicts native liver survival in biliary atresia

2019 ◽  
Vol 87 (4) ◽  
pp. 730-734 ◽  
Author(s):  
Cho-Yi Huang ◽  
Mei-Hwei Chang ◽  
Huey-Ling Chen ◽  
Yen-Hsuan Ni ◽  
Hong-Yuan Hsu ◽  
...  
Keyword(s):  
Biliary Atresia ◽  
Bilirubin Level ◽  
Native Liver ◽  
Level 1 ◽  
Native Liver Survival

Analysis of the prognostic factors of long-term native liver survival in survivors of biliary atresia

2016 ◽  
Vol 32 (9) ◽  
pp. 839-843 ◽  
Author(s):  
Hideyuki Sasaki ◽  
Hiromu Tanaka ◽  
Motoshi Wada ◽  
Takuro Kazama ◽  
Megumi Nakamura ◽  
...  
Keyword(s):  
Prognostic Factors ◽  
Biliary Atresia ◽  
Native Liver ◽  
Native Liver Survival

Ten-Year Native Liver Survival Rate After Laparoscopic and Open Kasai Portoenterostomy for Biliary Atresia

2019 ◽  
Vol 29 (1) ◽  
pp. 121-125 ◽  
Author(s):  
Kin Wai Edwin Chan ◽  
Kim Hung Lee ◽  
Hei Yi Vicky Wong ◽  
Siu Yan Bess Tsui ◽  
Jennifer Wai Cheung Mou ◽  
...  
Keyword(s):  
Survival Rate ◽  
Biliary Atresia ◽  
Kasai Portoenterostomy ◽  
Native Liver ◽  
Native Liver Survival

Hepatic expression of HGF/C-met and native liver survival in biliary atresia

2020 ◽  
Vol 36 (5) ◽  
pp. 597-602
Author(s):  
Panicha Tangtrongchitr ◽  
Sopee Poomsawat ◽  
Voranush Chongsrisawat ◽  
Sittisak Honsawek ◽  
Yong Poovorawan ◽  
...  
Keyword(s):  
Biliary Atresia ◽  
Hepatic Expression ◽  
Native Liver ◽  
Native Liver Survival

scholarly journals Postoperative intestinal obstruction in patients with biliary atresia impedes biliary excretion and results in subsequent liver transplantation

2021 ◽  
Author(s):  
Aitaro Takimoto ◽  
Wataru Sumida ◽  
Hizuru Amano ◽  
Chiyoe Shirota ◽  
Takahisa Tainaka ◽  
...  
Keyword(s):  
Intestinal Obstruction ◽  
Biliary Atresia ◽  
Postoperative Morbidity ◽  
Initial Operation ◽  
Negative Effects ◽  
Native Liver ◽  
Intestinal Obstructions ◽  
Postoperative Intestinal Obstruction ◽  
Free Status ◽  
Native Liver Survival

Abstract Purpose This study aimed to investigate the negative effects of intestinal obstruction for jaundice-free native liver survival after Kasai portoenterostomy (PE) for biliary atresia (BA). Methods We retrospectively reviewed the records of patients who underwent PE for BA between 2006 and 2019. We evaluated the postoperative morbidity of intestinal obstruction for up to 2 years after PE and the effects of intestinal obstruction on jaundice-free native liver survival. On the basis of their initial operation, patients were divided into open portoenterostomy (Open-PE) and laparoscopic portoenterostomy (Lap-PE) groups, and morbidity was compared. Results Of the 87 patients reviewed, 6 (6.9%) patients developed postoperative intestinal obstruction and underwent surgery to relieve the obstruction. The morbidity of early postoperative intestinal obstruction was 1.68 per 10,000 person days. The jaundice-free native liver survival rate among patients who once achieved jaundice-free status after PE was significantly lower in the patients with intestinal obstruction compared to in those without intestinal obstruction (0% vs. 73.8%; RR = 3.81, p = 0.007). No significant differences were seen in postoperative intestinal obstructions between the Open-PE and Lap-PE groups (p = 0.242). Conclusions Intestinal obstruction negatively impact jaundice-free native liver survival, even in patients who once achieved jaundice-free status after PE for BA.

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