IGLR-2, a Leucine-Rich Repeat Domain Containing Protein, Is Required for the Host Defense in Caenorhabditis elegans

Enterohemorrhagic Escherichia coli (EHEC), a human pathogen, also infects Caenorhabditis elegans. We demonstrated previously that C. elegans activates the p38 MAPK innate immune pathway to defend against EHEC infection. However, whether a C. elegans pattern recognition receptor (PRR) exists to regulate the immune pathway remains unknown. PRRs identified in other metazoans contain several conserved domains, including the leucine-rich repeat (LRR). By screening a focused RNAi library, we identified the IGLR-2, a transmembrane protein containing the LRR domain, as a potential immune regulator in C. elegans. Our data showed that iglr-2 regulates the host susceptibility to EHEC infection. Moreover, iglr-2 is required for pathogen avoidance to EHEC. The iglr-2 overexpressed strain, which was more resistant to EHEC originally, showed hypersusceptibility to EHEC upon knockdown of the p38 MAPK pathway. Together, our data suggested that iglr-2 plays an important role in C. elegans to defend EHEC by regulating pathogen-avoidance behavior and the p38 MAPK pathway.

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Conserved statin-mediated activation of the p38-MAPK pathway protects Caenorhabditis elegans from the cholesterol-independent effects of statins

Molecular Metabolism ◽

10.1016/j.molmet.2020.101003 ◽

2020 ◽

Vol 39 ◽

pp. 101003 ◽

Cited By ~ 1

Author(s):

Irina Langier Goncalves ◽

Sharon Tal ◽

Liza Barki-Harrington ◽

Amir Sapir

Keyword(s):

Caenorhabditis Elegans ◽

P38 Mapk ◽

Mapk Pathway ◽

P38 Mapk Pathway ◽

Independent Effects

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Caenorhabditis elegans mounts a p38 MAPK pathway‐mediated defence to Cutibacterium acnes infection

Cellular Microbiology ◽

10.1111/cmi.13234 ◽

2020 ◽

Vol 22 (10) ◽

Author(s):

Xiaowen Huang ◽

Wen Pan ◽

Wooseong Kim ◽

Alexis White ◽

Silei Li ◽

...

Keyword(s):

Caenorhabditis Elegans ◽

P38 Mapk ◽

Mapk Pathway ◽

P38 Mapk Pathway ◽

Cutibacterium Acnes

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Faculty Opinions recommendation of Microtubule depolymerization in Caenorhabditis elegans touch receptor neurons reduces gene expression through a p38 MAPK pathway.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.8934956.9491054 ◽

2011 ◽

Author(s):

Andrew Chisholm

Keyword(s):

Gene Expression ◽

Caenorhabditis Elegans ◽

P38 Mapk ◽

Mapk Pathway ◽

Microtubule Depolymerization ◽

P38 Mapk Pathway ◽

Touch Receptor Neurons ◽

Receptor Neurons

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Cranberry Extract Standardized for Proanthocyanidins Promotes the Immune Response of Caenorhabditis elegans to Vibrio cholerae through the p38 MAPK Pathway and HSF-1

PLoS ONE ◽

10.1371/journal.pone.0103290 ◽

2014 ◽

Vol 9 (7) ◽

pp. e103290 ◽

Cited By ~ 21

Author(s):

Jessica Dinh ◽

Joseph T. Angeloni ◽

Daniel B. Pederson ◽

Xiaoxia Wang ◽

Min Cao ◽

...

Keyword(s):

Immune Response ◽

Caenorhabditis Elegans ◽

Vibrio Cholerae ◽

P38 Mapk ◽

Mapk Pathway ◽

P38 Mapk Pathway

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Microtubule depolymerization in Caenorhabditis elegans touch receptor neurons reduces gene expression through a p38 MAPK pathway

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1101360108 ◽

2011 ◽

Vol 108 (10) ◽

pp. 3982-3987 ◽

Cited By ~ 63

Author(s):

A. Bounoutas ◽

J. Kratz ◽

L. Emtage ◽

C. Ma ◽

K. C. Nguyen ◽

...

Keyword(s):

Gene Expression ◽

Caenorhabditis Elegans ◽

P38 Mapk ◽

Mapk Pathway ◽

Microtubule Depolymerization ◽

P38 Mapk Pathway ◽

Touch Receptor Neurons ◽

Receptor Neurons

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Caenorhabditis elegans microRNAs of the let-7 family act in innate immune response circuits and confer robust developmental timing against pathogen stress

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1422858112 ◽

2015 ◽

Vol 112 (18) ◽

pp. E2366-E2375 ◽

Cited By ~ 51

Author(s):

Zhiji Ren ◽

Victor R. Ambros

Keyword(s):

Immune Response ◽

Caenorhabditis Elegans ◽

P38 Mapk ◽

Innate Immune Response ◽

Regulatory Networks ◽

Mapk Pathway ◽

Regulation Of Gene Expression ◽

Innate Immune ◽

Developmental Timing ◽

C Elegans

Animals maintain their developmental robustness against natural stresses through numerous regulatory mechanisms, including the posttranscriptional regulation of gene expression by microRNAs (miRNAs). Caenorhabditis elegans miRNAs of the let-7 family (let-7-Fam) function semiredundantly to confer robust stage specificity of cell fates in the hypodermal seam cell lineages. Here, we show reciprocal regulatory interactions between let-7-Fam miRNAs and the innate immune response pathway in C. elegans. Upon infection of C. elegans larvae with the opportunistic human pathogen Pseudomonas aeruginosa, the developmental timing defects of certain let-7-Fam miRNA mutants are enhanced. This enhancement is mediated by the p38 MAPK innate immune pathway acting in opposition to let-7-Fam miRNA activity, possibly via the downstream Activating Transcription Factor-7 (ATF-7). Furthermore, let-7-Fam miRNAs appear to exert negative regulation on the worm’s resistance to P. aeruginosa infection. Our results show that the inhibition of pathogen resistance by let-7 involves downstream heterochronic genes and the p38 MAPK pathway. These findings suggest that let-7-Fam miRNAs are integrated into innate immunity gene regulatory networks, such that this family of miRNAs modulates immune responses while also ensuring robust timing of developmental events under pathogen stress.

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The SEK-1 p38 MAP kinase pathway modulates Gq signaling in C. elegans

10.1101/093252 ◽

2016 ◽

Author(s):

Jill M. Hoyt ◽

Samuel K. Wilson ◽

Madhuri Kasa ◽

Jeremy S. Rise ◽

Irini Topalidou ◽

...

Keyword(s):

P38 Mapk ◽

Mapk Pathway ◽

P38 Map Kinase ◽

Mitogen Activated Protein Kinase ◽

Positive Role ◽

C Elegans ◽

Downstream Target ◽

P38 Mapk Pathway ◽

Mitogen Activated Protein ◽

Mature Neurons

AbstractGq is a heterotrimeric G protein that is widely expressed in neurons and regulates neuronal activity. To identify pathways regulating neuronal Gq signaling we performed a forward genetic screen in Caenorhabditis elegans for suppressors of activated Gq. One of the suppressors is an allele of sek-1, which encodes a mitogen-activated protein kinase kinase (MAPKK) in the p38 MAPK pathway. Here we show that sek-1 mutants have a slow locomotion rate and that sek-1 acts in acetylcholine neurons to modulate both locomotion rate and Gq signaling. Furthermore, we find that sek-1 acts in mature neurons to modulate locomotion. Using genetic and behavioral approaches we demonstrate that other components of the p38 MAPK pathway also play a positive role in modulating locomotion and Gq signaling. Finally, we find that mutants in the SEK-1 p38 MAPK pathway partially suppress an activated mutant of the sodium leak channel NCA-1/NALCN, a downstream target of Gq signaling. Our results suggest that the SEK-1 p38 pathway may modulate the output of Gq signaling through NCA-1.

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Lactobacillus casei triggers a TLR mediated RACK-1 dependent p38 MAPK pathway in Caenorhabditis elegans to resist Klebsiella pneumoniae infection

Food & Function ◽

10.1039/c6fo00510a ◽

2016 ◽

Vol 7 (7) ◽

pp. 3211-3223 ◽

Cited By ~ 13

Author(s):

Arumugam Kamaladevi ◽

Krishnaswamy Balamurugan

Keyword(s):

Lactic Acid ◽

Caenorhabditis Elegans ◽

Lactic Acid Bacteria ◽

Klebsiella Pneumoniae ◽

P38 Mapk ◽

Lactobacillus Casei ◽

Molecular Level ◽

Mapk Pathway ◽

Model Organism ◽

P38 Mapk Pathway

In the present study, the effect of lactic acid bacteria was investigated at the molecular level using the model organismCaenorhabditis elegansagainstKlebsiella pneumoniae.

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