scholarly journals Apelin Promotes Endothelial Progenitor Cell Angiogenesis in Rheumatoid Arthritis Disease via the miR-525-5p/Angiopoietin-1 Pathway

2021 ◽  
Vol 12 ◽  
Author(s):  
Ting-Kuo Chang ◽  
You-Han Zhong ◽  
Shan-Chi Liu ◽  
Chien-Chung Huang ◽  
Chun-Hao Tsai ◽  
...  

Angiogenesis is a critical process in the formation of new capillaries and a key participant in rheumatoid arthritis (RA) pathogenesis. The adipokine apelin (APLN) plays critical roles in several cellular functions, including angiogenesis. We report that APLN treatment of RA synovial fibroblasts (RASFs) increased angiopoietin-1 (Ang1) expression. Ang1 antibody abolished endothelial progenitor cell (EPC) tube formation and migration in conditioned medium from APLN-treated RASFs. We also found significantly higher levels of APLN and Ang1 expression in synovial fluid from RA patients compared with those with osteoarthritis. APLN facilitated Ang1-dependent EPC angiogenesis by inhibiting miR-525-5p synthesis via phospholipase C gamma (PLCγ) and protein kinase C alpha (PKCα) signaling. Importantly, infection with APLN shRNA mitigated EPC angiogenesis, articular swelling, and cartilage erosion in ankle joints of mice with collagen-induced arthritis. APLN is therefore a novel therapeutic target for RA.

2021 ◽  
Vol 12 (9) ◽  
Author(s):  
Chun-Hao Tsai ◽  
Chao-Ju Chen ◽  
Chi-Li Gong ◽  
Shan-Chi Liu ◽  
Po-Chun Chen ◽  
...  

AbstractAngiogenesis is a critical process in the formation of new capillaries and a key participant in rheumatoid arthritis (RA) pathogenesis. The chemokine (C-X-C motif) ligand 13 (CXCL13) plays important roles in several cellular functions such as infiltration, migration, and motility. We report significantly higher levels of CXCL13 expression in collagen-induced arthritis (CIA) mice compared with controls and also in synovial fluid from RA patients compared with human osteoarthritis (OA) samples. RA synovial fluid increased endothelial progenitor cell (EPC) homing and angiogenesis, which was blocked by the CXCL13 antibody. By interacting with the CXCR5 receptor, CXCL13 facilitated vascular endothelial growth factor (VEGF) expression and angiogenesis in EPC through the PLC, MEK, and AP-1 signaling pathways. Importantly, infection with CXCL13 short hairpin RNA (shRNA) mitigated EPC homing and angiogenesis, articular swelling, and cartilage erosion in ankle joints of mice with CIA. CXCL13 is therefore a novel therapeutic target for RA.


2015 ◽  
Vol 240 (1) ◽  
pp. 131-136 ◽  
Author(s):  
Javier Rodríguez-Carrio ◽  
Mercedes Alperi-López ◽  
Patricia López ◽  
Sara Alonso-Castro ◽  
Santiago Rubén Carro-Esteban ◽  
...  

2012 ◽  
Vol 76 (1) ◽  
pp. 172-175 ◽  
Author(s):  
Eunju O ◽  
Seung Woo LEE ◽  
Hyun-Sun LEE ◽  
Hee-Suk LIM ◽  
Hyun-Young AHN ◽  
...  

2004 ◽  
Vol 199 (3) ◽  
pp. 60
Author(s):  
Sundeep G. Keswani ◽  
Jignesh K. Parvadia ◽  
David Stitelman ◽  
Elliot D. Kozin ◽  
Antoneta P. Radu ◽  
...  

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Matthew David Silverman ◽  
Christian S Haas ◽  
Ali M Rad ◽  
Ali S Arbab ◽  
Alisa E Koch

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