scholarly journals Plastidial and ER Triacylglycerol Biosynthesis in a Growth Phase-Dependent Manner in the Heterokont Nannochloropsis oceanica

2020 ◽  
Vol 7 ◽  
Author(s):  
Dan Huang ◽  
Da-Wei Li ◽  
Srinivasan Balamurugan ◽  
Jian-Wei Zheng ◽  
Wan-Jun Liu ◽  
...  
Keyword(s):  
Growth Phase ◽  
Dependent Manner ◽  
Nannochloropsis Oceanica ◽  
Triacylglycerol Biosynthesis

scholarly journals Differential Expression ofVibrio vulnificusElastase Gene in a Growth Phase-dependent Manner by Two Different Types of Promoters

2001 ◽  
Vol 276 (17) ◽  
pp. 13875-13880 ◽  
Author(s):  
Hye Sook Jeong ◽  
Kwang Cheol Jeong ◽  
Hyun Kyung Choi ◽  
Kyung-Je Park ◽  
Kyu-Ho Lee ◽  
...  
Keyword(s):  
Differential Expression ◽  
Growth Phase ◽  
Dependent Manner ◽  
Different Types

scholarly journals Glucose Triggers ATP Secretion from Bacteria in a Growth-Phase-Dependent Manner

2013 ◽  
Vol 79 (7) ◽  
pp. 2328-2335 ◽  
Author(s):  
Ippei Hironaka ◽  
Tadayuki Iwase ◽  
Shinya Sugimoto ◽  
Ken-ichi Okuda ◽  
Akiko Tajima ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Growth Phase ◽  
Cell Function ◽  
Immune Cell ◽  
Dependent Manner ◽  
T Helper 17 ◽  
Content Type ◽  
Cell Functions ◽  
Atp Secretion ◽  
Enterococcus Gallinarum

ABSTRACTATP modulates immune cell functions, and ATP derived from gut commensal bacteria promotes the differentiation of T helper 17 (Th17) cells in the intestinal lamina propria. We recently reported thatEnterococcus gallinarum, isolated from mice and humans, secretes ATP. We have since found and characterized several ATP-secreting bacteria. Of the tested enterococci,Enterococcus mundtiisecreted the greatest amount of ATP (>2 μM/108cells) after overnight culture. Glucose, not amino acids and vitamins, was essential for ATP secretion fromE. mundtii. Analyses of energy-deprived cells demonstrated that glycolysis is the most important pathway for bacterial ATP secretion. Furthermore, exponential-phaseE. mundtiiandEnterococcus faecaliscells secrete ATP more efficiently than stationary-phase cells. Other bacteria, includingPseudomonas aeruginosa,Escherichia coli, andStaphylococcus aureus, also secrete ATP in exponential but not stationary phase. These results suggest that various gut bacteria, including commensals and pathogens, might secrete ATP at any growth phase and modulate immune cell function.

scholarly journals Role of ςB in Adaptation ofListeria monocytogenes to Growth at Low Temperature

2000 ◽  
Vol 182 (24) ◽  
pp. 7083-7087 ◽  
Author(s):  
Lynne A. Becker ◽  
Stefanie N. Evans ◽  
Robert W. Hutkins ◽  
Andrew K. Benson
Keyword(s):  
Listeria Monocytogenes ◽  
Low Temperature ◽  
Growth Phase ◽  
Cold Shock ◽  
Dependent Manner ◽  
High Osmolarity ◽  
Efficient Uptake ◽  
Oflisteria Monocytogenes

ABSTRACT The activity of ςB in Listeria monocytogenes is stimulated by high osmolarity and is necessary for efficient uptake of osmoprotectants. Here we demonstrate that, during cold shock, ςB contributes to adaptation in a growth phase-dependent manner and is necessary for efficient accumulation of betaine and carnitine as cryoprotectants.

scholarly journals TouR-Mediated Effector-Independent Growth Phase-Dependent Activation of the σ54 Ptou Promoter of Pseudomonas stutzeri OX1

2004 ◽  
Vol 186 (21) ◽  
pp. 7353-7363 ◽  
Author(s):  
Dafne Solera ◽  
Fabio L. G. Arenghi ◽  
Tanja Woelk ◽  
Enrica Galli ◽  
Paola Barbieri
Keyword(s):  
Growth Phase ◽  
Nitrogen Starvation ◽  
Pseudomonas Stutzeri ◽  
Dependent Manner ◽  
Native Strain ◽  
Regulatory Circuit ◽  
Peculiar Characteristic ◽  
Promoter Swapping ◽  
Necessary And Sufficient ◽  
First Time

ABSTRACT Transcription of the catabolic touABCDEF operon, encoding the toluene-o-xylene monooxygenase of Pseudomonas stutzeri OX1, is driven by the σ54-dependent Ptou promoter, whose activity is controlled by the phenol-responsive NtrC-like activator TouR. In this paper we describe for the first time a peculiar characteristic of this system, namely, that Ptou transcription is activated in a growth phase-dependent manner in the absence of genuine effectors of the cognate TouR regulator. This phenomenon, which we named gratuitous activation, was observed in the native strain P. stutzeri OX1, as well as in a Pseudomonas putida PaW340 host harboring the reconstructed tou regulatory circuit. Regulator-promoter swapping experiments demonstrated that the presence of TouR is necessary and sufficient for imposing gratuitous activation on the Ptou promoter, as well as on other σ54-dependent catabolic promoters, whereas the highly similar phenol-responsive activator DmpR is unable to activate the Ptou promoter in the absence of effectors. We show that this phenomenon is specifically triggered by carbon source exhaustion but not by nitrogen starvation. An updated model of the tou regulatory circuit is presented.

scholarly journals Synthetic LPETG-Containing Peptide Incorporation in the Staphylococcus aureus Cell-Wall in a Sortase A- and Growth Phase-Dependent Manner

PLoS ONE ◽  
2014 ◽  
Vol 9 (2) ◽  
pp. e89260 ◽  
Author(s):  
Silvie Hansenová Maňásková ◽  
Kamran Nazmi ◽  
Alex van Belkum ◽  
Floris J. Bikker ◽  
Willem J. B. van Wamel ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Wall ◽  
Growth Phase ◽  
Sortase A ◽  
Dependent Manner

scholarly journals The Listeria monocytogenes prfAP2 promoter is regulated by sigma B in a growth phase dependent manner

2005 ◽  
Vol 245 (2) ◽  
pp. 329-336 ◽  
Author(s):  
Ute Schwab ◽  
Barbara Bowen ◽  
Celine Nadon ◽  
Martin Wiedmann ◽  
Kathryn J. Boor
Keyword(s):  
Listeria Monocytogenes ◽  
Growth Phase ◽  
Dependent Manner ◽  
Sigma B

scholarly journals SarA Represses agr Operon Expression in a Purified In Vitro Staphylococcus aureusTranscription System

2000 ◽  
Vol 182 (20) ◽  
pp. 5893-5897 ◽  
Author(s):  
Swarup K. Chakrabarti ◽  
Tapan K. Misra
Keyword(s):  
Experimental Data ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Rna Polymerase ◽  
Growth Phase ◽  
Surface Protein ◽  
Dependent Manner ◽  
Operon Expression ◽  
Chromosomal Loci

ABSTRACT Mutation and genetic complementation studies suggested that two chromosomal loci, agr and sar, are involved in the upregulation of several exotoxin genes and the downregulation of a number of surface protein genes in a growth phase-dependent manner inStaphylococcus aureus. We purified recombinant T7-tagged SarA from Escherichia coli and determined its effect on transcription from several S. aureus promoters by using purified RNA polymerase reconstituted with either ςA or ςB from S. aureus. Of the seven ςA-dependent promoters that we tested, SarA repressed transcription from agrP2, agrP3,cna, sarP1, and sea promoters and did not affect sec and znt promoters. Furthermore, SarA had no effect on transcription from the ςB-dependent sarP3 promoter. In vitro experimental data presented in this report suggest that SarA expression is autoregulated.

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