scholarly journals Recent Developments of the Synthetic Biology Toolkit for Clostridium

2018 ◽  
Vol 9 ◽  
Author(s):  
Rochelle C. Joseph ◽  
Nancy M. Kim ◽  
Nicholas R. Sandoval
2019 ◽  
Vol 2 (2) ◽  
pp. 39 ◽  
Author(s):  
Dohyun Jeong ◽  
Melissa Klocke ◽  
Siddharth Agarwal ◽  
Jeongwon Kim ◽  
Seungdo Choi ◽  
...  

Synthetic biology integrates diverse engineering disciplines to create novel biological systems for biomedical and technological applications. The substantial growth of the synthetic biology field in the past decade is poised to transform biotechnology and medicine. To streamline design processes and facilitate debugging of complex synthetic circuits, cell-free synthetic biology approaches has reached broad research communities both in academia and industry. By recapitulating gene expression systems in vitro, cell-free expression systems offer flexibility to explore beyond the confines of living cells and allow networking of synthetic and natural systems. Here, we review the capabilities of the current cell-free platforms, focusing on nucleic acid-based molecular programs and circuit construction. We survey the recent developments including cell-free transcription–translation platforms, DNA nanostructures and circuits, and novel classes of riboregulators. The links to mathematical models and the prospects of cell-free synthetic biology platforms will also be discussed.


Science ◽  
2011 ◽  
Vol 333 (6047) ◽  
pp. 1252-1254 ◽  
Author(s):  
Petra Schwille

How synthetic can “synthetic biology” be? A literal interpretation of the name of this new life science discipline invokes expectations of the systematic construction of biological systems with cells being built module by module—from the bottom up. But can this possibly be achieved, taking into account the enormous complexity and redundancy of living systems, which distinguish them quite remarkably from design features that characterize human inventions? There are several recent developments in biology, in tight conjunction with quantitative disciplines, that may bring this literal perspective into the realm of the possible. However, such bottom-up engineering requires tools that were originally designed by nature’s greatest tinkerer: evolution.


2020 ◽  
Vol 12 (1) ◽  
pp. 321-345
Author(s):  
Adam Searle

Abstract The spectacle of de-extinction is often forward facing at the interface of science fiction and speculative fact, haunted by extinction’s pasts. Missing from this discourse, however, is a robust theorization of de-extinction in the present. This article presents recent developments in the emergent fields of resurrection biology and liminality to conceptualize the anabiotic (not living nor dead) state of de/extinction. Through two stories, this article explores the epistemological perturbation caused by the suspended animation of genetic material. Contrasting the genomic stories of the bucardo, a now extinct subspecies of Iberian ibex whose genome was preserved before the turn of the millennium, and the woolly mammoth, whose genome is still a work in progress, the author poses questions concerning the existential authenticity of this genomic anabiosis. They serve as archetypal illustrations of salvaged and synthesized anabiotic creatures. De/extinction is presented as a liminal state of being, both living and dead, both fact and fiction, a realm that we have growing access to through the proliferation of synthetic biology and cryopreservation. The article concludes through a presentation of anabiotic geographies, postulating on the changing biocultural significances we attach to organisms both extinct and extant, and considering their implications for the contemporary extinction crisis.


Author(s):  
Massimiliano Simons ◽  

In this article, two different claims about nature are discussed. On the one hand, environmental philosophy has forced us to reflect on our position within nature. We are not the masters of nature as was claimed before. On the other hand there are the recent developments within synthetic biology. It claims that, now at last, we can be the masters of nature we have never been before. The question is then raised how these two claims must be related to one another. Rather than stating that they are completely irreconcilable, I will argue for a dialogue aimed to discuss the differences and similarities. The claim is that we should not see it as two successive temporal phases of our relation to nature, but two tendencies that can coexist.


2018 ◽  
Vol 28 (3) ◽  
pp. 290-304 ◽  
Author(s):  
Robert Meckin ◽  
Andrew Balmer

Public involvement in technological anticipation is a common feature of contemporary sociotechnical innovation. However, most engagements abstract sociotechnical futures, rather than situating them in the everyday practices in which people are routinely engaged. Recent developments in synthetic biology have established the potential for ‘drop in’ replacements for ingredients in consumer products, particularly in flavour and fragrance markets. This article explains how a sensory methodology can be used to explore citizens’ everyday experiences and how these can be used to ground anticipation of possible sociotechnical futures. The article uses a socio-historical approach to analyse and compare two practice domains – caring for families and hygiene and personal care – to show how biosynthetic futures can disrupt existing relations between people, objects and ideas. The implications for conceptualising publics in synthetic biology and for approaches to public engagement and participation are discussed more broadly.


2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Sheeja Jagadevan ◽  
Avik Banerjee ◽  
Chiranjib Banerjee ◽  
Chandan Guria ◽  
Rameshwar Tiwari ◽  
...  

2017 ◽  
Vol 1861 (11) ◽  
pp. 2945-2947
Author(s):  
Patrick O’Donoghue ◽  
Ilka U. Heinemann

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Lorenzo Olivi ◽  
Mareike Berger ◽  
Ramon N. P. Creyghton ◽  
Nicola De Franceschi ◽  
Cees Dekker ◽  
...  

AbstractRecent developments in synthetic biology may bring the bottom-up generation of a synthetic cell within reach. A key feature of a living synthetic cell is a functional cell cycle, in which DNA replication and segregation as well as cell growth and division are well integrated. Here, we describe different approaches to recreate these processes in a synthetic cell, based on natural systems and/or synthetic alternatives. Although some individual machineries have recently been established, their integration and control in a synthetic cell cycle remain to be addressed. In this Perspective, we discuss potential paths towards an integrated synthetic cell cycle.


Author(s):  
Priyanka Patel Vatsa ◽  
Manju Shri ◽  
Poonam Bhargava ◽  
Chaitanya G. Joshi ◽  
Madhvi Joshi

Author(s):  
Kai Tong ◽  
G. Ozan Bozdag ◽  
William C. Ratcliff

In order to understand the evolution of multicellularity, we must understand how and why selection favors the first steps in this process: the evolution of simple multicellular groups. Multicellularity has evolved many times in independent lineages with fundamentally different ecologies, yet no work has yet systematically examined these diverse selective drivers. Here we review recent developments in systematics, comparative biology, paleontology, synthetic biology, theory, and experimental evolution, highlighting ten selective drivers of simple multicellularity. Our survey highlights the many ecological opportunities available for simple multicellularity, and stresses the need for additional work examining how these first steps impact the subsequent evolution of complex multicellularity.


Sign in / Sign up

Export Citation Format

Share Document