scholarly journals Effects of Continuous Cropping of Sweet Potato on the Fungal Community Structure in Rhizospheric Soil

2019 ◽  
Vol 10 ◽  
Author(s):  
Zhiyuan Gao ◽  
Meikun Han ◽  
Yaya Hu ◽  
Ziqian Li ◽  
Chaofang Liu ◽  
...  
Keyword(s):  
Community Structure ◽  
Sweet Potato ◽  
Fungal Community ◽  
Continuous Cropping ◽  
Rhizospheric Soil ◽  
Fungal Community Structure

scholarly journals Effects of Continuous Cropping on Bacterial Community Structure in Rhizospheric Soil of Sweet Potato 

2020 ◽  
Author(s):  
Zhiyuan Gao ◽  
Yaya Hu ◽  
Meikun Han ◽  
Junjie Xu ◽  
Xue Wang ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Sweet Potato ◽  
Soil Ph ◽  
Bacterial Community Structure ◽  
Continuous Cropping ◽  
Rhizospheric Soil ◽  
Sweet Potatoes ◽  
Potato Varieties ◽  
Soil Bacterial Community Structure

Abstract Background: Continuous cropping obstacles from sweet potato are widespread, which seriously reduce the yield and quality, cause certain economic losses. Bacteria of Rhizospheric soil are the richest and are associated with obstacles to continuous cropping. However, few studies on how continuous sweet potato cropping affects the rhizospheric soil bacterial community structure. In the study, Illumina Miseq method was used to explore rhizosphere soil bacterial community structure changes with different sweet potato varieties, and the correlation between soil characteristics and this bacterial community after continuous cropping, to provide theoretical guidance for prevention and treatment of sweet potatoes continuous cropping obstacles. Results: After continuous cropping two years, the results showed that (1) the dominant bacterial phlya in rhizospheric soils from both Xushu18 and Yizi138 were Proteobacteria, Acidobacteria, and Actinobacteria. The most dominant genus was Subgroup 6_norank. The relative abundance of rhizospheric soil bacteria of two sweet potato varieties changed significantly. (2) The richness and diversity indexes of bacteria in Xushu18 rhizospheric soil were higher than those from Yizi138 after continuous cropping. Moreover, the beneficial Lysobacter and Bacillus were more prevalent in Xushu18, but Yizi138 contained more harmful Gemmatimonadetes. (3) Soil pH decreased after continuous cropping, and redundancy analysis result indicated that soil pH was correlated significantly with bacterial community. Spearman’s rank correlations coefficients analysis demonstrated that pH was positively associated with Planctomycetes and Acidobacteria, but negatively associated with Actinobacteria and Firmicutes.Conclusions: After continuous cropping, the bacterial community structure and physicochemical properties of sweet potato rhizospheric soil were unbalanced, and the changes from different sweet potato varieties were different. The contents of Lysobacter and Bacillus were higher in the sweet potato variety resistant to continuous cropping. It provides a basis for developing new microbial fertilizer for sweet potatoes to alleviate continuous cropping obstacle.

scholarly journals Effects of Continuous Cropping on Bacterial Community Structure in Rhizospheric Soil of Sweet Potato

2020 ◽  
Author(s):  
Zhiyuan Gao ◽  
Yaya Hu ◽  
Meikun Han ◽  
Junjie Xu ◽  
Xue Wang ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Sweet Potato ◽  
Soil Ph ◽  
Bacterial Community Structure ◽  
High Throughput Sequencing ◽  
Potato Industry ◽  
Continuous Cropping ◽  
Rhizospheric Soil ◽  
Potato Varieties

Abstract Background: Continuous cropping obstacles from sweet potato are widespread, which seriously reduce the yield and quality, restrict the sustainable development of sweet potato industry. Bacteria are the most abundant in rhizospheric soil and have a certain relationship with continuous cropping obstacles. However, there are few reports on how continuous cropping affected the bacterial community structure in the rhizospheric soil of sweet potato. In this study, high-throughput sequencing technique was used to explore the changes of rhizospheric soil bacterial community structure of different sweet potato varieties, and the correlation between soil characteristics and this bacterial community after continuous cropping, so as to provide a theoretical basis for the prevention and control of sweet potato continuous cropping obstacles.Results: After two years of continuous cropping, the results showed that (1) the dominant bacteria phlya in rhizospheric soils from both Xushu18 and Yizi138 were Proteobacteria, Acidobacteria, and Actinobacteria. The most dominant genus was Subgroup 6_norank. Significant changes in the relative abundance of rhizospheric soil bacteria were observed for two sweet potato varieties. (2) Bacterial richness and diversity indexes of rhizospheric soil from Xushu18 were higher than those from Yizi138 after continuous cropping. Moreover, the beneficial Lysobacter and Bacillus were more prevalent in Xushu18, but Yizi138 contained more harmful Gemmatimonadetes. (3) Soil pH decreased after continuous cropping, and redundancy analysis showed that soil pH was significantly correlated with bacterial community. Spearman’s rank correlations coefficients analysis demonstrated that pH was positively correlated with Planctomycetes and Acidobacteria, and negatively correlated with Actinobacteria and Firmicutes.Conclusions: After continuous cropping of sweet potato, the bacterial community structure and physicochemical properties in the rhizospheric soil were unbalanced, and the changes of different sweet potato varieties were different. The contents of Lysobacter and Bacillus were higher in the sweet potato variety resistant to continuous cropping. It provides a basis for the development of special microbial fertilizer for sweet potatoes to alleviate continuous cropping obstacle.

scholarly journals Effects of Continuous Cropping of Sweet Potatoes on the Bacterial Community Structure in Rhizospheric Soil

2020 ◽  
Author(s):  
Zhiyuan Gao ◽  
Yaya Hu ◽  
Meikun Han ◽  
Junjie Xu ◽  
Xue Wang ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Sweet Potato ◽  
Soil Ph ◽  
Bacterial Community Structure ◽  
Continuous Cropping ◽  
Rhizospheric Soil ◽  
Sweet Potatoes ◽  
Potato Varieties ◽  
Soil Bacterial Community Structure

Abstract Background: Continuous cropping obstacles from sweet potatoes are widespread, which seriously reduce the yield and quality, causing certain economic losses. Bacteria of rhizospheric soil are the richest and are associated with obstacles to continuous cropping. However, few studies have examined how continuous sweet potato cropping affects the rhizospheric soil bacterial community structure. Results: In the study, the Illumina MiSeq method was used to explore the variations inrhizospheric soil bacterial community structure of different sweet potato varieties after continuous cropping, as well as the correlation between soil characteristics and the bacterial community. The results showed that (1) the dominant bacterial phyla in rhizospheric soils from both Xushu 18 and Yizi 138 were Proteobacteria, Acidobacteria, and Actinobacteria. The most dominant genus was Subgroup 6_norank. The relative abundance of rhizospheric soil bacteria varied significantly between the two sweet potato varieties. (2) The richness and diversity indexes of bacteria were higher in Xushu 18 rhizospheric soil than in Yizi 138 soil after continuous cropping. Moreover, beneficial Lysobacter and Bacillus were more prevalent in Xushu 18, while Yizi 138 contained more harmful Gemmatimonadetes. (3) Soil pH decreased after continuous cropping, and redundancy analysis indicated that soil pH was significantly correlated with the bacterial community. Spearman’s rank correlation coefficient analysis demonstrated that pH was positively associated with Planctomycetes and Acidobacteria, but negatively associated with Actinobacteria and Firmicutes.Conclusions: After continuous cropping, the bacterial community structure and physicochemical properties of sweet potato rhizospheric soil were changed, and the changes from different sweet potato varieties were different. The contents of Lysobacter and Bacillus were higher in the sweet potato variety resistant to continuous cropping. It provides a basis for developing new microbial fertilizers for sweet potatoes to alleviate the continuous cropping obstacle.

scholarly journals Dynamics of soil properties and fungal community structure in continuous-cropped alfalfa fields in Northeast China

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7127 ◽  
Author(s):  
Qin Yao ◽  
Yanxia Xu ◽  
Xuefeng Liu ◽  
Junjie Liu ◽  
Xinyu Huang ◽  
...  
Keyword(s):  
Community Structure ◽  
Soil Properties ◽  
Fungal Community ◽  
Continuous Cropping ◽  
Fungal Community Composition ◽  
Fungal Community Structure ◽  
P Content ◽  
Soil Fungal Community ◽  
Relative Abundances ◽  
Total P

To compensate for the seasonal imbalance between livestock and forage yield in the cold region of Northeast China, alfalfa (Medicago sativa L.) continuous cropping has been widely employed in animal husbandry. However, the effects of continuous cropping of alfalfa on soil properties, including physical, chemical and biological properties, are poorly understood. In this study, we investigated the soil properties and fungal community composition of alfalfa fields under continuous cropping for different time periods (i.e., 1, 2, 6, 9, 12, 13 and 35 years). The results showed that soil moisture, total C, total N, NO3−-N and available K content decreased at less than 10 years of continuous cropping and then increased at more than 10 years of continuous cropping, but soil total P and available P content showed the opposite tendency. The soil fungal community composition determined using Illumina Miseq sequencing showed that continuous cropping increased the fungal alpha diversity and changed the fungal community structure. The relative abundances of Guehomyces and Chaetomium decreased, but the relative abundances of Phaeomycocentrospora and Paecilomyces increased with continuous cropping time. In addition, continuous cropping of alfalfa increased the relative abundances of some plant pathogens, such as Haematonectria haematococca and Cyphellophora sp. Soil total P and available P content were important soil factors affecting the soil fungal community diversity, fungal community structure and the relative abundances of specific fungi in this alfalfa continuous cropping system.

scholarly journals Response of soil fungal communities to continuous cropping of flue-cured tobacco

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shengnan Wang ◽  
Jiangke Cheng ◽  
Tong Li ◽  
Yuncheng Liao
Keyword(s):  
Community Structure ◽  
Fungal Community ◽  
High Throughput Sequencing ◽  
Cropping Systems ◽  
Quantitative Polymerase Chain Reaction ◽  
Fungal Communities ◽  
Continuous Cropping ◽  
Fungal Community Structure ◽  
Soil Variables ◽  
Fungal Abundance

AbstractFungal communities are considered to be critically important for crop health and soil fertility. However, our knowledge of the response of fungal community structure to the continuous cropping of flue-cured tobacco is limited, and the interaction of soil fungal communities under different cropping systems remains unclear. In this study, we comparatively investigated the fungal abundance, diversity, and community composition in the soils in which continuous cropping of flue-cured tobacco for 3 years (3ys), 5 years (5ys), and cropping for 1 year (CK) using quantitative polymerase chain reaction and high-throughput sequencing technology. The results revealed that continuous cropping of flue-cured tobacco changed the abundance of soil fungi, and caused a significant variation in fungal diversity. In particular, continuous cropping increased the relative abundance of Mortierellales, which can dissolve mineral phosphorus in soil. Unfortunately, continuous cropping also increased the risk of potential pathogens. Moreover, long-term continuous cropping had more complex and stabilize network. This study also indicated that available potassium and available phosphorous were the primary soil factors shifting the fungal community structure. These results suggested that several soil variables may affect fungal community structure. The continuous cropping of flue-cured tobacco significantly increased the abundance and diversity of soil fungal communities.

scholarly journals Different Fertilizers Applied Alter Fungal Community Structure in Rhizospheric Soil of Cassava (Manihot esculenta Crantz) and Increase Crop Yield

2021 ◽  
Vol 12 ◽  
Author(s):  
Jie Cai ◽  
Jie Zhang ◽  
Yun Ding ◽  
Shan Yu ◽  
Hongxin Lin ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Plant Growth ◽  
Microbial Community Structure ◽  
Fungal Community ◽  
Pathogenic Fungi ◽  
Growth And Yield ◽  
Rhizospheric Soil ◽  
Fungal Community Structure ◽  
Beneficial Fungi

Soil microbes play an important role in the ecosystem and have a relationship with plant growth, development, and production. There are only a few reports on the effects of planting patterns of cassava on the microbial community structure in the rhizospheric soil. Here, we investigated the effects of different fertilization on the microbial community structure in the cassava rhizospheric soil. SC205 cultivar was used in this study as the experimental material. Compound fertilizer (CF) and reduced fertilizer (RF) were applied to the soil prior to planting. Soil samples were collected before harvest, and fungi were analyzed using IonS5TMXL sequencing platform. Results showed that CF and RF treatments significantly increased cassava yield. Amplicon sequencing result indicated that the fungi richness in rhizospheric soil of cassava was increased after CF was applied, and the diversity was decreased. However, the fungal diversity and richness were decreased in rhizospheric soil after RF was applied. The most dominant fungal phylum was Ascomycota, which increased after fertilization. In addition, the abundance of beneficial fungi such as Chaetomium increased after fertilization, while that of pathogenic fungi such as Fusarium solani was decreased. The composition of the fungal community in rhizospheric soil with CF and RF applied was similar, but the richness and diversity of fungi were different. Canonical correspondence analysis (CCA) indicates there was a positive correlation between soil nutrition and fungal community structure. Overall, our results indicate that fertilization alters the fungal community structure of cassava rhizospheric soil, such that the abundance of potentially beneficial fungi increased, while that of potentially pathogenic fungi decreased, thereby significantly promoting plant growth and yield of cassava. Thus, during actual production, attention should be paid to maintain the stability of cassava rhizospheric soil micro-ecology.

scholarly journals Effects of Continuous Cropping of Lilium Lancifolium on Rhizosphere Soil Physical and Chemical Properties and Fungal Community Structure

2021 ◽  
Author(s):  
Shixia Huang ◽  
Lihao Lin ◽  
Tianyu Zhao ◽  
Yi Yuan ◽  
Yuling Tai ◽  
...  
Keyword(s):  
Community Structure ◽  
Fungal Community ◽  
Rhizosphere Soil ◽  
Chemical Properties ◽  
Illumina Miseq ◽  
Continuous Cropping ◽  
Fungal Community Structure ◽  
Lilium Lancifolium ◽  
Physical And Chemical ◽  
Beneficial Fungi

Abstract Lilium lancifolium is an important economic crop in Huoshan county of Anhui province, China. Continuous cropping obstacles serious affect the yield and quality of L. lancifolium. At present, the effect of the continuous cropping of L. lancifolium on soil fungal community structure is not clear. In this study, Illumina MiSeq was used to study the fungi of the rhizosphere soil associated with L. lancifolium subjected to three treatments: no continuous cropping, continuous cropping for 3 years, and continuous cropping for 5 years. The results showed that continuous cropping of L. lancifolium could increase the fungal richness and diversity in the rhizosphere to different degrees. Ascomycota was the dominant phylum, and its abundance increased after continuous cropping. In addition, the abundance of beneficial fungi, such as Chaetomium, decreased, and the abundance of harmful fungi, such as Fusarium and Colletotrichum, greatly increased with the duration of continuous cropping. Overall, continuous cropping changed the composition of soil fungal communities, reduced the abundance of beneficial fungi, and increased the abundance of harmful fungi. Thus, continuous cropping increased the potential for soil-borne diseases and endangered the bulb growth of L. lancifolium.

scholarly journals Effects of different years of natural recovery of Gastrodia elata on the community structure of bacteria and fungi in rhizosphere soil

2021 ◽  
Author(s):  
Long lan ping Long ◽  
Luo fu lai Luo
Keyword(s):  
Community Structure ◽  
Total Nitrogen ◽  
Fungal Community ◽  
Rhizosphere Soil ◽  
Continuous Cropping ◽  
Gastrodia Elata ◽  
Fungal Community Structure ◽  
Natural Restoration ◽  
Increasing Trend ◽  
Bacteria And Fungi

Abstract Background: There are serious obstacles to continuous cropping of Gastrodia elata. After continuous cropping of Gastrodia elata, the yield will be greatly reduced, or even no harvest. Soil nutrients and microorganisms play an important role in it. There are few related studies on the effects of natural restoration of the rhizosphere soil of Gastrodia elata on the structure of soil bacteria and fungi for different years.Results: In this study, a combination of Illumina Miseq library preparation and high-throughput sequencing technology was used. The soil that had not been planted with Gastrodia elata was used as a control to study the characteristics of soil microbial communities in the rhizosphere of Gastrodia elata from 0 to 5 years of natural restoration, and Correlation between soil characteristics and fungal and bacterial communities. The results showed that (1) The dominant bacteria in the rhizosphere soil of Gastrodia elata are roteobacteria, Actinobacteria and Acidobacteria, and the most important bacterial genera are norank_f_Xanthobacteraceae and Bradyrhizobium. The dominant fungal phyla are Basidiomycota and Ascomycota, and the main fungal genera are Mortierella and Trichoderma. The bacterial and fungal community composition in the rhizosphere soil of Gastrodia elata and the soil without Gastrodia elata planted are not much different, but the community structure and relative abundance are quite different. (2) The Chao index, Shannon index and Invsimpson index of Gastrodia elata rhizosphere soil show an increasing trend with the increase of the natural restoration period, indicating that natural restoration for a certain period of time will increase the diversity and abundance of bacteria and fungi in the rhizosphere soil of Gastrodia elata Increase. (3) With the increase of the natural restoration period, the total nitrogen and pH content of the rhizosphere soil of Gastrodia elata showed an increasing trend. total nitrogen has the greatest influence on the bacterial community structure, and the influence of pH on the fungal community structure ranks No. Second, in the top three phyla with relative content, total nitrogen is positively correlated with Actinobacteria, and pH is positively correlated with Mortierellomycota.Conclusion: After 3 years of natural restoration, the soil environment gradually returns to normal, and the microbial community tends to balance; in addition, the Spearman rank correlation coefficient analysis shows that total nitrogen has the greatest impact on the bacterial community structure in the rhizosphere soil of Gastrodia elata, and total phosphorus has the greatest impact on the fungal community structure. The results provide a theoretical basis for the development of new type microbial fertilizers and soil amendments for Gastrodia elata to alleviate continuous cropping obstacles.

Sign in / Sign up

Export Citation Format

Share Document