scholarly journals Effects of Continuous Cropping of Lilium Lancifolium on Rhizosphere Soil Physical and Chemical Properties and Fungal Community Structure

2021 ◽  
Author(s):  
Shixia Huang ◽  
Lihao Lin ◽  
Tianyu Zhao ◽  
Yi Yuan ◽  
Yuling Tai ◽  
...  
Keyword(s):  
Community Structure ◽  
Fungal Community ◽  
Rhizosphere Soil ◽  
Chemical Properties ◽  
Illumina Miseq ◽  
Continuous Cropping ◽  
Fungal Community Structure ◽  
Lilium Lancifolium ◽  
Physical And Chemical ◽  
Beneficial Fungi

Abstract Lilium lancifolium is an important economic crop in Huoshan county of Anhui province, China. Continuous cropping obstacles serious affect the yield and quality of L. lancifolium. At present, the effect of the continuous cropping of L. lancifolium on soil fungal community structure is not clear. In this study, Illumina MiSeq was used to study the fungi of the rhizosphere soil associated with L. lancifolium subjected to three treatments: no continuous cropping, continuous cropping for 3 years, and continuous cropping for 5 years. The results showed that continuous cropping of L. lancifolium could increase the fungal richness and diversity in the rhizosphere to different degrees. Ascomycota was the dominant phylum, and its abundance increased after continuous cropping. In addition, the abundance of beneficial fungi, such as Chaetomium, decreased, and the abundance of harmful fungi, such as Fusarium and Colletotrichum, greatly increased with the duration of continuous cropping. Overall, continuous cropping changed the composition of soil fungal communities, reduced the abundance of beneficial fungi, and increased the abundance of harmful fungi. Thus, continuous cropping increased the potential for soil-borne diseases and endangered the bulb growth of L. lancifolium.

Comparative Analysis of Fungal Diversity and Community Structure of Different Volcanic Soils in Wudalianchi, China

2021 ◽  
Vol 50 (2) ◽  
pp. 327-334
Author(s):  
Fan Yang ◽  
Liqiang Mu ◽  
Qingyang Huang ◽  
Lihong Xie ◽  
Hongjie Cao ◽  
...  
Keyword(s):  
Community Structure ◽  
Environmental Factors ◽  
Fungal Community ◽  
Fungal Diversity ◽  
Chemical Properties ◽  
Fungal Community Structure ◽  
Α Diversity ◽  
Physical And Chemical ◽  
The Relationship ◽  
Soil Environmental Factors

The relationship between the fungal community characteristics and soil environmental factors of volcanic ecosystem in Wudalianchi, China were investigated. The soil fungal community structure and diversity of new, old, and non-erupting volcanos were explored through highthroughput sequencing technology. The result showed that the physical and chemical properties of three plots were significantly different. Through sequencing 578 species, 366 genera, 202 families, 89 orders, 32 classes, and 11 phyla were detected. Among them Ascomycota and Basidiomycota were the dominant fungi phyla. The relative abundance of various flora determined by phylum classification showed significant differences. The Shannon, Simpson, Ace, and Chao1 indices for the soil fungi in the three plots were also significantly different. Redundancy and correlation analyses showed that the α diversity of fungi was significantly correlated with pH, organic matter and total nitrogen in the soil. These results indicate that soil environmental factors influence the fungal diversity in the different volcanic ecosystems in Wudalianchi, China. Bangladesh J. Bot. 50(2): 327-334, 2021 (June)

scholarly journals Effects of different years of natural recovery of Gastrodia elata on the community structure of bacteria and fungi in rhizosphere soil

2021 ◽  
Author(s):  
Long lan ping Long ◽  
Luo fu lai Luo
Keyword(s):  
Community Structure ◽  
Total Nitrogen ◽  
Fungal Community ◽  
Rhizosphere Soil ◽  
Continuous Cropping ◽  
Gastrodia Elata ◽  
Fungal Community Structure ◽  
Natural Restoration ◽  
Increasing Trend ◽  
Bacteria And Fungi

Abstract Background: There are serious obstacles to continuous cropping of Gastrodia elata. After continuous cropping of Gastrodia elata, the yield will be greatly reduced, or even no harvest. Soil nutrients and microorganisms play an important role in it. There are few related studies on the effects of natural restoration of the rhizosphere soil of Gastrodia elata on the structure of soil bacteria and fungi for different years.Results: In this study, a combination of Illumina Miseq library preparation and high-throughput sequencing technology was used. The soil that had not been planted with Gastrodia elata was used as a control to study the characteristics of soil microbial communities in the rhizosphere of Gastrodia elata from 0 to 5 years of natural restoration, and Correlation between soil characteristics and fungal and bacterial communities. The results showed that (1) The dominant bacteria in the rhizosphere soil of Gastrodia elata are roteobacteria, Actinobacteria and Acidobacteria, and the most important bacterial genera are norank_f_Xanthobacteraceae and Bradyrhizobium. The dominant fungal phyla are Basidiomycota and Ascomycota, and the main fungal genera are Mortierella and Trichoderma. The bacterial and fungal community composition in the rhizosphere soil of Gastrodia elata and the soil without Gastrodia elata planted are not much different, but the community structure and relative abundance are quite different. (2) The Chao index, Shannon index and Invsimpson index of Gastrodia elata rhizosphere soil show an increasing trend with the increase of the natural restoration period, indicating that natural restoration for a certain period of time will increase the diversity and abundance of bacteria and fungi in the rhizosphere soil of Gastrodia elata Increase. (3) With the increase of the natural restoration period, the total nitrogen and pH content of the rhizosphere soil of Gastrodia elata showed an increasing trend. total nitrogen has the greatest influence on the bacterial community structure, and the influence of pH on the fungal community structure ranks No. Second, in the top three phyla with relative content, total nitrogen is positively correlated with Actinobacteria, and pH is positively correlated with Mortierellomycota.Conclusion: After 3 years of natural restoration, the soil environment gradually returns to normal, and the microbial community tends to balance; in addition, the Spearman rank correlation coefficient analysis shows that total nitrogen has the greatest impact on the bacterial community structure in the rhizosphere soil of Gastrodia elata, and total phosphorus has the greatest impact on the fungal community structure. The results provide a theoretical basis for the development of new type microbial fertilizers and soil amendments for Gastrodia elata to alleviate continuous cropping obstacles.

scholarly journals Comparison of rhizosphere soil microbial diversity of different introduced alfalfa varieties

2020 ◽  
Author(s):  
Chunhui Ma ◽  
Jiangjiao Qi ◽  
Xue Yu ◽  
Lihe Su ◽  
Tingting He ◽  
...  
Keyword(s):  
Community Structure ◽  
Fungal Community ◽  
High Throughput Sequencing ◽  
Bacterial Species ◽  
Chemical Properties ◽  
Animal Husbandry ◽  
Desert Soil ◽  
High Yield ◽  
Soil Microbial Diversity ◽  
Fungal Community Structure

Abstract Alfalfa (Medicago sativa L.) is an important forage legume in farming and animal husbandry systems. In this study, MiSeq high-throughput sequencing was applied to assess the relationship between bacterial and fungal community structures and alfalfa growth characteristics and soil physical and chemical properties induced by different cultivars alfalfa (Victoria, Kangsai, Aohan) in the grey desert soil. The results showed that the diversity of bacterial and fungal in Victoria was higher, and the bacterial diversity was significantly lower for alfalfa with Aohan than for the others, and the fungal diversity was lower for alfalfa with Kangsai than for the others. Heatmap showed that total nitrogen, fresh weight, pH and organic have significantly affect fungal community structure, whereas pH and organic carbon also significant effects on bacterial community structure. LefSe analysis showed that the growth adaptability of introduced alfalfa is mainly related to fungal and bacterial species, and the beneficial microorganisms with significant differences and relative high abundance are significantly enriched in Victoria. Pathogens with high relative abundance are mainly concentrated in Aohan alfalfa soil. Based on our findings, Victoria is the high-yield alfalfa suitable for planting in gray desert soil, while planting Kangsai and Aohan alfalfa needs probiotic for adjuvant.

scholarly journals Promoting the Growth of Pinus sylvestris var. mongolica Seedlings and Improving Rhizosphere Fungal Community Structure through Interaction between Trichoderma and Ectomycorrhizal Fungi

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Saiyaremu Halifu ◽  
Xun Deng ◽  
Jun Zhang ◽  
Jiangbao Xia ◽  
Xiaoshuang Song ◽  
...  
Keyword(s):  
Organic Matter ◽  
Community Structure ◽  
Pinus Sylvestris ◽  
Total Phosphorus ◽  
Ectomycorrhizal Fungi ◽  
Fungal Community ◽  
Trichoderma Harzianum ◽  
Rhizosphere Soil ◽  
Suillus Luteus ◽  
Fungal Community Structure

In this study, pot experiments were conducted on the seedlings of Pinus sylvestris var. mongolica to study the influence of Trichoderma (Trichoderma harzianum E15) and Ectomycorrhizal fungi (Suillus luteus N94) on the growth of these seedlings. In particular, the effects of these fungi on the fungal community structure in the rhizosphere soil of the seedlings were investigated. Inoculation with Trichoderma harzianum E15 and Suillus luteus N94 significantly (P < 0.05) promoted the growth of the Pinus sylvestris seedlings. The non-metric multidimensional scaling (NMDS) results indicated a significant difference (P < 0.05) between the fungal community structures in the rhizosphere soil of the annual and biennial seedlings. In the rhizosphere soil of annual seedlings, the main fungi were Ascomycota, Basidiomycota, Zygomycota. Ascomycota, Basidiomycota, Mortierellomycota, and p-unclassified-k-Fungi were the main fungi in the rhizosphere soil of biennial seedlings. The dominant genus in the rhizosphere soil and a key factor promoting the growth of the annual and the biennial seedlings was Trichoderma, Suillus, respectively. Both of them were negatively correlated with the relative abundance of microbial flora in the symbiotic environment. Trichoderma had a significant promoting effect on the conversion of total phosphorus, total nitrogen, ammonium nitrogen, nitrate nitrogen, and the organic matter in the rhizosphere soil of the seedlings, while Suillus significantly promoted the conversion of organic matter and total phosphorus.

scholarly journals Dynamics of soil properties and fungal community structure in continuous-cropped alfalfa fields in Northeast China

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7127 ◽  
Author(s):  
Qin Yao ◽  
Yanxia Xu ◽  
Xuefeng Liu ◽  
Junjie Liu ◽  
Xinyu Huang ◽  
...  
Keyword(s):  
Community Structure ◽  
Soil Properties ◽  
Fungal Community ◽  
Continuous Cropping ◽  
Fungal Community Composition ◽  
Fungal Community Structure ◽  
P Content ◽  
Soil Fungal Community ◽  
Relative Abundances ◽  
Total P

To compensate for the seasonal imbalance between livestock and forage yield in the cold region of Northeast China, alfalfa (Medicago sativa L.) continuous cropping has been widely employed in animal husbandry. However, the effects of continuous cropping of alfalfa on soil properties, including physical, chemical and biological properties, are poorly understood. In this study, we investigated the soil properties and fungal community composition of alfalfa fields under continuous cropping for different time periods (i.e., 1, 2, 6, 9, 12, 13 and 35 years). The results showed that soil moisture, total C, total N, NO3−-N and available K content decreased at less than 10 years of continuous cropping and then increased at more than 10 years of continuous cropping, but soil total P and available P content showed the opposite tendency. The soil fungal community composition determined using Illumina Miseq sequencing showed that continuous cropping increased the fungal alpha diversity and changed the fungal community structure. The relative abundances of Guehomyces and Chaetomium decreased, but the relative abundances of Phaeomycocentrospora and Paecilomyces increased with continuous cropping time. In addition, continuous cropping of alfalfa increased the relative abundances of some plant pathogens, such as Haematonectria haematococca and Cyphellophora sp. Soil total P and available P content were important soil factors affecting the soil fungal community diversity, fungal community structure and the relative abundances of specific fungi in this alfalfa continuous cropping system.

scholarly journals Response of soil fungal communities to continuous cropping of flue-cured tobacco

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shengnan Wang ◽  
Jiangke Cheng ◽  
Tong Li ◽  
Yuncheng Liao
Keyword(s):  
Community Structure ◽  
Fungal Community ◽  
High Throughput Sequencing ◽  
Cropping Systems ◽  
Quantitative Polymerase Chain Reaction ◽  
Fungal Communities ◽  
Continuous Cropping ◽  
Fungal Community Structure ◽  
Soil Variables ◽  
Fungal Abundance

AbstractFungal communities are considered to be critically important for crop health and soil fertility. However, our knowledge of the response of fungal community structure to the continuous cropping of flue-cured tobacco is limited, and the interaction of soil fungal communities under different cropping systems remains unclear. In this study, we comparatively investigated the fungal abundance, diversity, and community composition in the soils in which continuous cropping of flue-cured tobacco for 3 years (3ys), 5 years (5ys), and cropping for 1 year (CK) using quantitative polymerase chain reaction and high-throughput sequencing technology. The results revealed that continuous cropping of flue-cured tobacco changed the abundance of soil fungi, and caused a significant variation in fungal diversity. In particular, continuous cropping increased the relative abundance of Mortierellales, which can dissolve mineral phosphorus in soil. Unfortunately, continuous cropping also increased the risk of potential pathogens. Moreover, long-term continuous cropping had more complex and stabilize network. This study also indicated that available potassium and available phosphorous were the primary soil factors shifting the fungal community structure. These results suggested that several soil variables may affect fungal community structure. The continuous cropping of flue-cured tobacco significantly increased the abundance and diversity of soil fungal communities.

scholarly journals Continuous Cropping Regulates Fungal Community Structures and Functional Groups in Rhizosphere Soil of Tibet Barley

2020 ◽  
Author(s):  
Youhua Yao ◽  
Yuan Zhao ◽  
Xiaohua Yao ◽  
Yixiong Bai ◽  
Likun An ◽  
...  
Keyword(s):  
Community Structure ◽  
High Altitude ◽  
Fungal Community ◽  
Plant Pathogens ◽  
Rhizosphere Soil ◽  
Rrna Gene ◽  
Continuous Cropping ◽  
Ecological Groups ◽  
Fungal Plant Pathogens ◽  
Endophyte Fungi

Abstract Continuous cropping regulates the community structure of rhizosphere soil microbes; however, the effects of continuous cropping on the community structure and function of rhizosphere fungal communities of plants cultivated in high-altitude regions are not well understood. In this study, 18S rRNA gene high-throughput sequencing was applied to examine the rhizosphere fungal community structure during continuous cropping of Tibet barley (a principal cereal cultivated on the Qinghai-Tibetan Plateau). The results showed that the Chao1 and phylogenetic diversity (PD) indices declined as cropping years increased. Additionally, relative abundance of the genera Cystofilobasidium, Mucor, and Ustilago increased, whereas the abundance of Fusarium decreased during continuous cropping. Furthermore, identification of ecological groups using FUNGuild revealed that saprotrophs, pathogens, and symbiotrophs were the dominant groups in rhizosphere soil, and these three trophic modes all increased significantly with continuous cropping. During continuous cropping, the fungal plant-pathogens Parastagonospora and Ustilago also increased remarkably, as did the endophyte fungi Verticillium. Collectively, continuous cropping of Tibet barely increased the potential for plant-pathogenic and endophyte fungi in rhizosphere soil. Thus, the development of sustainable farming practices to reduce the abundance of harmful fungi is vital to Tibet barley growth and production during continuous cropping of plants cultivated in high-altitude regions.

scholarly journals Different Fertilizers Applied Alter Fungal Community Structure in Rhizospheric Soil of Cassava (Manihot esculenta Crantz) and Increase Crop Yield

2021 ◽  
Vol 12 ◽  
Author(s):  
Jie Cai ◽  
Jie Zhang ◽  
Yun Ding ◽  
Shan Yu ◽  
Hongxin Lin ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Plant Growth ◽  
Microbial Community Structure ◽  
Fungal Community ◽  
Pathogenic Fungi ◽  
Growth And Yield ◽  
Rhizospheric Soil ◽  
Fungal Community Structure ◽  
Beneficial Fungi

Soil microbes play an important role in the ecosystem and have a relationship with plant growth, development, and production. There are only a few reports on the effects of planting patterns of cassava on the microbial community structure in the rhizospheric soil. Here, we investigated the effects of different fertilization on the microbial community structure in the cassava rhizospheric soil. SC205 cultivar was used in this study as the experimental material. Compound fertilizer (CF) and reduced fertilizer (RF) were applied to the soil prior to planting. Soil samples were collected before harvest, and fungi were analyzed using IonS5TMXL sequencing platform. Results showed that CF and RF treatments significantly increased cassava yield. Amplicon sequencing result indicated that the fungi richness in rhizospheric soil of cassava was increased after CF was applied, and the diversity was decreased. However, the fungal diversity and richness were decreased in rhizospheric soil after RF was applied. The most dominant fungal phylum was Ascomycota, which increased after fertilization. In addition, the abundance of beneficial fungi such as Chaetomium increased after fertilization, while that of pathogenic fungi such as Fusarium solani was decreased. The composition of the fungal community in rhizospheric soil with CF and RF applied was similar, but the richness and diversity of fungi were different. Canonical correspondence analysis (CCA) indicates there was a positive correlation between soil nutrition and fungal community structure. Overall, our results indicate that fertilization alters the fungal community structure of cassava rhizospheric soil, such that the abundance of potentially beneficial fungi increased, while that of potentially pathogenic fungi decreased, thereby significantly promoting plant growth and yield of cassava. Thus, during actual production, attention should be paid to maintain the stability of cassava rhizospheric soil micro-ecology.

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