Inhibitory Effects of Carbazomycin B Produced by Streptomyces roseoverticillatus 63 Against Xanthomonas oryzae pv. oryzae

The present manuscript highlights the potential role of Streptomyces roseoverticillatus 63 (Sr-63) against Xanthomonas oryzae pv. oryzae (Xoo), which is the cause of a disastrous bacterial leaf blight disease with rice worldwide. The disease suppression was achieved under greenhouse conditions. A foliar spray of the fermentation broth of Sr-63 significantly reduced the leaf blight symptoms with rice in Xoo inoculated rice plants. Furthermore, we observed that the carbazomycin B, isolated from the fermentation broth of Sr-63, was demonstrated to have antibacterial activity against Xoo with a minimum inhibitory concentration (MIC) of 8 μg mL–1. The results indicated that carbzomycin B hampered the membrane formation of Xoo, reduced the production of xanthomonadin and extracellular polymeric substance (EPS). The fourier transform infrared spectroscopic (FT-IR) indicated that carbazomycin B changed the components of the cell membrane, then caused a change of the cell surface hydrophobicity of Xoo. Scanning electron microscopy revealed that the Xoo cells treated with carbazomycin B exhibited apparent structural deformation. The results also indicated that carbazomycin B had a negative impact on the metabolism of Xoo, carbazomycin B reduced the activity of malate dehydrogenase (MDH) activity and suppressed the protein expression of Xoo. Overall, our data suggests that Streptomyces roseoverticillatus 63 is a promising biocontrol agent that could be used to combat the bacterial leaf blight diseases of rice.

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Biogenic silver nanoparticles as an antibacterial agent against bacterial leaf blight causing rice phytopathogen Xanthomonas oryzae pv. oryzae

Bioprocess and Biosystems Engineering ◽

10.1007/s00449-021-02579-7 ◽

2021 ◽

Author(s):

Karunakar Reddy Namburi ◽

Aruna Jyothi Kora ◽

Anuradha Chetukuri ◽

Vijaya Sree Meena Kumari Kota

Keyword(s):

Silver Nanoparticles ◽

Antibacterial Agent ◽

Leaf Blight ◽

Xanthomonas Oryzae ◽

Bacterial Leaf Blight ◽

Biogenic Silver Nanoparticles

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Response of a local rice variety Pandanwangi to infection of bacterial leaf blight Xanthomonas oryzae pv. oryzae strain III, IV, and VIII

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/905/1/012068 ◽

2021 ◽

Vol 905 (1) ◽

pp. 012068

Author(s):

Hadiwiyono ◽

S H Poromarto ◽

S Widono ◽

R F Rizal

Keyword(s):

Rice Variety ◽

Leaf Blight ◽

Rice Production ◽

Xanthomonas Oryzae ◽

Bacterial Leaf Blight ◽

Artificial Inoculation ◽

Limiting Factors ◽

Bacterial Suspension ◽

West Java ◽

Local Cultivar

Abstract Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the limiting factors in rice production. A local cultivar, rice “Pandanwangi” is a superior variety much preferred and cultivated by the farmers in Cianjur, West Java, Indonesia. The information about the response of “Pandangwangi” to Xoo is still poorly understood. This paper reports the results of the evaluation of “Pandanwangi” response against BLB. This research was conducted in a greenhouse with artificial inoculation using Xoo strains III, IV, and VIII with bacterial suspension at 108 cfu.mL−1. The results showed that the response of cv Pandanwangi to Xoo infection was different from the strain of Xoo. “Pandanwangi” cultivar was susceptible to Xoo strain III and VIII and very susceptible to strain IV.

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Studies on the ecological properties of Xanthomonas oryzae (Uyeda et Ishiyama) Dowson, the causal organism of bacterial leaf blight of rice plant

Japanese Journal of Phytopathology ◽

10.3186/jjphytopath.28.107 ◽

1963 ◽

Vol 28 (3) ◽

pp. 107-107

Author(s):

Takeyuki MIZUKAMI

Keyword(s):

Rice Plant ◽

Leaf Blight ◽

Xanthomonas Oryzae ◽

Bacterial Leaf Blight ◽

Causal Organism

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Discovery of Novel Dihydrolipoamide S-Succinyltransferase Inhibitors Based on Fragment Virtual Screening

International Journal of Molecular Sciences ◽

10.3390/ijms222312953 ◽

2021 ◽

Vol 22 (23) ◽

pp. 12953

Author(s):

Chengqian Wei ◽

Junjie Huang ◽

Yu Wang ◽

Yifang Chen ◽

Xin Luo ◽

...

Keyword(s):

Virtual Screening ◽

Antibacterial Agents ◽

High Efficiency ◽

Leaf Blight ◽

Xanthomonas Oryzae ◽

Bacterial Leaf Blight ◽

Extracellular Polysaccharides ◽

Bacterial Leaf Streak ◽

Bacterial Diseases ◽

Sulfone Derivatives

A series of new oxadiazole sulfone derivatives containing an amide moiety was synthesized based on fragment virtual screening to screen high-efficiency antibacterial agents for rice bacterial diseases. All target compounds showed greater bactericidal activity than commercial bactericides. 3-(4-fluorophenyl)-N-((5-(methylsulfonyl)-1,3,4-oxadiazol-2-yl)methyl)acrylamide (10) showed excellent antibacterial activity against Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola, with EC50 values of 0.36 and 0.53 mg/L, respectively, which were superior to thiodiazole copper (113.38 and 131.54 mg/L) and bismerthiazol (83.07 and 105.90 mg/L). The protective activity of compound 10 against rice bacterial leaf blight and rice bacterial leaf streak was 43.2% and 53.6%, respectively, which was superior to that of JHXJZ (34.1% and 26.4%) and thiodiazole copper (33.0% and 30.2%). The curative activity of compound 10 against rice bacterial leaf blight and rice bacterial leaf streak was 44.5% and 51.7%, respectively, which was superior to that of JHXJZ (32.6% and 24.4%) and thiodiazole copper (27.1% and 28.6%). Moreover, compound 10 might inhibit the growth of Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola by affecting the extracellular polysaccharides, destroying cell membranes, and inhibiting the enzyme activity of dihydrolipoamide S-succinyltransferase.

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Riboflavin (Vitamin B2) mediated defence induction against bacterial leaf blight: probing through chlorophyll a fluorescence induction O–J–I–P transients

Functional Plant Biology ◽

10.1071/fp18117 ◽

2018 ◽

Vol 45 (12) ◽

pp. 1251 ◽

Cited By ~ 1

Author(s):

Shasmita ◽

Harekrushna Swain ◽

Anuprita Ray ◽

Pradipta K. Mohapatra ◽

Ramani K. Sarkar ◽

...

Keyword(s):

Chlorophyll Fluorescence ◽

Oryza Sativa L ◽

Bacterial Pathogen ◽

Fluorescence Induction ◽

Leaf Blight ◽

Xanthomonas Oryzae ◽

Bacterial Leaf Blight ◽

Yield Data ◽

Progress Curve ◽

Moderately Resistant

Bacterial leaf blight (BLB) is a serious threat for rice (Oryza sativa L.) cultivation caused by the bacterial pathogen Xanthomonas oryzae pv. oryzae. The pathogen mainly damages the leaf chlorophyllous tissue, resulting in poor photosynthesis and causing up to 50% reductions in grain yield. In the present work, we have compared the structural and functional ability of the chloroplast of three varieties of rice with different degrees of susceptibility (TN1, highly susceptible; IR-20, moderately resistant; DV-85, resistant to BLB) treated with riboflavin (1 and 2 mM) and infected with BLB, with chlorophyll fluorescence as a tool. As indicated by the chlorophyll fluorescence technique, the disease progress curve and yield data, riboflavin acted as an effective vitamin for inducing resistance against BLB. Plants treated with riboflavin showed improved PSII activity, more chlorophyll content and higher yield than the diseased plants.

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