scholarly journals Metagenomic Insights Into Functional and Taxonomic Compositions of an Activated Sludge Microbial Community Treating Leachate of a Completed Landfill: A Pathway-Based Analysis

2021 ◽  
Vol 12 ◽  
Author(s):  
Shohei Yasuda ◽  
Toshikazu Suenaga ◽  
Laura Orschler ◽  
Shelesh Agrawal ◽  
Susanne Lackner ◽  
...  
Keyword(s):  
Microbial Community ◽  
Activated Sludge ◽  
Mixed Culture ◽  
Landfill Leachate ◽  
Treatment Plant ◽  
Metabolic Potential ◽  
Leachate Treatment ◽  
Microbial Taxonomy ◽  
Engineered Systems ◽  
Metagenome Sequencing

Upcycling wastes into valuable products by mixed microbial communities has recently received considerable attention. Sustainable production of high-value substances from one-carbon (C1) compounds, e.g., methanol supplemented as an external electron donor in bioreactors for wastewater treatment, is a promising application of upcycling. This study undertook a gene-centric approach to screen valuable production potentials from mixed culture biomass, removing organic carbon and nitrogen from landfill leachate. To this end, the microbial community of the activated sludge from a landfill leachate treatment plant and its metabolic potential for the production of seven valuable products were investigated. The DNA extracted from the activated sludge was subjected to shotgun metagenome sequencing to analyze the microbial taxonomy and functions associated with producing the seven products. The functional analysis confirmed that the activated sludge could produce six of the valuable products, ectoine, polyhydroxybutyrate (PHB), zeaxanthin, astaxanthin, acetoin, and 2,3-butanediol. Quantification of the detected functional gene hit numbers for these valuable products as a primary trial identified a potential rate-limiting metabolic pathway, e.g., conversion of L-2,4-diaminobutyrate into N-γ-acetyl-L2,4,-diaminobutyrate during the ectoine biosynthesis. Overall, this study demonstrated that primary screening by the proposed gene-centric approach can be used to evaluate the potential for the production of valuable products using mixed culture or single microbe in engineered systems. The proposed approach can be expanded to sites where water purification is highly required, but resource recovery, or upcycling has not been implemented.

Enhancing filterability of activated sludge from landfill leachate treatment plant by applying electrical field ineffective on bacterial life

2017 ◽  
Vol 24 (11) ◽  
pp. 10364-10372 ◽  
Author(s):  
Gulizar Kurtoglu Akkaya ◽  
Elif Sekman ◽  
Selin Top ◽  
Ece Sagir ◽  
Mehmet Sinan Bilgili ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Landfill Leachate ◽  
Treatment Plant ◽  
Leachate Treatment ◽  
Electrical Field

scholarly journals Identification of a Metagenome-Assembled Genome of an Uncultured Methyloceanibacter sp. Strain Acquired from an Activated Sludge System Used for Landfill Leachate Treatment

2020 ◽  
Vol 9 (32) ◽  
Author(s):  
Shohei Yasuda ◽  
Toshikazu Suenaga ◽  
Laura Orschler ◽  
Shelesh Agrawal ◽  
Susanne Lackner ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Genome Sequence ◽  
Landfill Leachate ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Landfill Site ◽  
Leachate Treatment ◽  
Total Size ◽  
Activated Sludge System ◽  
Metagenome Sequencing

ABSTRACT Using metagenome sequencing, a nearly complete genome sequence was retrieved for the uncultured Methyloceanibacter sp. strain A49, recovered from an activated sludge system used for landfill leachate treatment at a closed landfill site. The total size and encoded sequences are 3,407,434 bp and 3,280 genes, respectively.

scholarly journals Niche Differentiation among Three Closely RelatedCompetibacteraceaeClades at a Full-Scale Activated Sludge Wastewater Treatment Plant and Putative Linkages to Process Performance

2018 ◽  
Vol 85 (5) ◽  
Author(s):  
Veronica R. Brand ◽  
Laurel D. Crosby ◽  
Craig S. Criddle
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Niche Differentiation ◽  
Full Scale ◽  
Pure Oxygen ◽  
Operational Parameters ◽  
Metabolic Potential ◽  
Operational Conditions

ABSTRACTMultiple clades within a microbial taxon often coexist within natural and engineered environments. Because closely related clades have similar metabolic potential, it is unclear how diversity is sustained and what factors drive niche differentiation. In this study, we retrieved three near-complete Competibacter lineage genomes from activated sludge metagenomes at a full-scale pure oxygen activated sludge wastewater treatment plant. The three genomes represent unique taxa within theCompetibacteraceae. A comparison of the genomes revealed differences in capacity for exopolysaccharide (EPS) biosynthesis, glucose fermentation to lactate, and motility. Using quantitative PCR (qPCR), we monitored these clades over a 2-year period. The clade possessing genes for motility and lacking genes for EPS biosynthesis (CPB_P15) was dominant during periods of suspended solids in the effluent. Further analysis of operational parameters indicate that the dominance of the CPB_P15 clade is associated with low-return activated sludge recycle rates and low wasting rates, conditions that maintain relatively high levels of biomass within the system.IMPORTANCEMembers of the Competibacter lineage are relevant in biotechnology as glycogen-accumulating organisms (GAOs). Here, we document the presence of threeCompetibacteraceaeclades in a full-scale activated sludge wastewater treatment plant and their linkage to specific operational conditions. We find evidence for niche differentiation among the three clades with temporal variability in clade dominance that correlates with operational changes at the treatment plant. Specifically, we observe episodic dominance of a likely motile clade during periods of elevated effluent turbidity, as well as episodic dominance of closely related nonmotile clades that likely enhance floc formation during periods of low effluent turbidity.

Anodic Oxidation of Effluents from Stages of MBR-UF Municipal Landfill Leachate Treatment Plant

2020 ◽  
Vol 37 (10) ◽  
pp. 702-714
Author(s):  
Zubeda Ukundimana ◽  
Mehmet Kobya ◽  
Philip Isaac Omwene ◽  
Erhan Gengec ◽  
Orhan Taner Can
Keyword(s):  
Anodic Oxidation ◽  
Landfill Leachate ◽  
Treatment Plant ◽  
Leachate Treatment ◽  
Municipal Landfill

Pilot study for the potential application of a shortcut nitrification and denitrification process in landfill leachate treatment with MBR

2006 ◽  
Vol 6 (6) ◽  
pp. 147-154 ◽  
Author(s):  
K.J. An ◽  
J.W. Tan ◽  
L. Meng
Keyword(s):  
Pilot Study ◽  
Landfill Leachate ◽  
Ammonia Oxidation ◽  
Carbon Sources ◽  
Treatment Plant ◽  
Optimum Operating ◽  
Leachate Treatment ◽  
Optimum Operating Condition ◽  
Nitrification And Denitrification ◽  
Denitrification Process

An advanced nitrogen removal pilot study was performed in China's Xia Ping Landfill Leachate Treatment Plant to undertake shortcut nitrification and denitrification with the Membrane Bio-reactor (MBR) process. It was found that the MBR process used 25% less of the oxygen and 40% less of the external carbon sources, compared to the conventional nitrification and denitrification process. The key feature of the MBR process is that it provides an environment more favorable for ammonia oxidation bacterium (AOB) than for nitrite oxidation bacterium (NOB) through controlling loading, pH, temperature, dissolved oxygen concentration (DO), and NH3 inhibition. Optimum operating condition was examined through continuous running of the pilot MBR, and it was found that a minimum HRT of 4.3 days and maximum ammonia loading of 0.6 kg N- NH4+ m3.d with pH 7–8.5, temperature 25–30 °C, and DO at 2 mg/L is favorable to AOB. Kinetic study was conducted to identify the characteristic of the microorganisms in the system. Measured Ks and μA,max of MBR sludge was 19.65 mg NH4-N/L (Temperature 25 °C, pH 8.5) and 0.26 d−1, respectively.

scholarly journals Activated Sludge Microbial Community and Treatment Performance of Wastewater Treatment Plants in Industrial and Municipal Zones

2020 ◽  
Vol 17 (2) ◽  
pp. 436 ◽  
Author(s):  
Yongkui Yang ◽  
Longfei Wang ◽  
Feng Xiang ◽  
Lin Zhao ◽  
Zhi Qiao
Keyword(s):  
Wastewater Treatment ◽  
Microbial Community ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Wastewater Sludge ◽  
Community Diversity ◽  
Nitrogen And Phosphorus ◽  
Metabolic Functions

Controlling wastewater pollution from centralized industrial zones is important for reducing overall water pollution. Microbial community structure and diversity can adversely affect wastewater treatment plant (WWTP) performance and stability. Therefore, we studied microbial structure, diversity, and metabolic functions in WWTPs that treat industrial or municipal wastewater. Sludge microbial community diversity and richness were the lowest for the industrial WWTPs, indicating that industrial influents inhibited bacterial growth. The sludge of industrial WWTP had low Nitrospira populations, indicating that influent composition affected nitrification and denitrification. The sludge of industrial WWTPs had high metabolic functions associated with xenobiotic and amino acid metabolism. Furthermore, bacterial richness was positively correlated with conventional pollutants (e.g., carbon, nitrogen, and phosphorus), but negatively correlated with total dissolved solids. This study was expected to provide a more comprehensive understanding of activated sludge microbial communities in full-scale industrial and municipal WWTPs.

Sign in / Sign up

Export Citation Format

Share Document