Genome Analysis of Multidrug-Resistant Shewanella algae Isolated From Human Soft Tissue Sample

Shewanella soft tissue infections usually occur in immunocompromised patients with a preexisting cutaneous ulcer, mostly after exposure to a marine environment or contaminated water. A 35-year-old male presented with a non-healing ulcer over the distal end of his right leg but had no predisposing factors. Cultures of exudates from the wound grew Shewanella on repeated occasions. Recovery was uneventful following surgical debridement and antimicrobial therapy. Early suspicion, diagnosis, and treatment with potent antibiotics are needed to prevent any further complications resulting from infection by this emerging pathogen.

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Genome analysis of two multidrug-resistant Escherichia coli O8:H9-ST48 strains isolated from lettuce

Gene ◽

10.1016/j.gene.2021.145603 ◽

2021 ◽

pp. 145603

Author(s):

Susana Araújo ◽

Marta Tacão ◽

Rafael Baraúna ◽

Rommel Ramos ◽

Artur Silva ◽

...

Keyword(s):

Escherichia Coli ◽

Genome Analysis ◽

Multidrug Resistant

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Patterns and variability of tumor oxygenation in human soft tissue sarcomas, cervical carcinomas, and lymph node metastases

International Journal of Radiation Oncology*Biology*Physics ◽

10.1016/0360-3016(95)00106-9 ◽

1995 ◽

Vol 32 (4) ◽

pp. 1121-1125 ◽

Cited By ~ 98

Author(s):

David M. Brizel ◽

Gary L. Rosner ◽

Leonard R. Prosnitz ◽

Mark W. Dewhirst

Keyword(s):

Lymph Node ◽

Soft Tissue ◽

Lymph Node Metastases ◽

Soft Tissue Sarcomas ◽

Tumor Oxygenation ◽

Cervical Carcinomas ◽

Human Soft Tissue ◽

Node Metastases

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PO-1921 Radiosensitizing effect of Trabectidin on human soft tissue sarcoma cells

Radiotherapy and Oncology ◽

10.1016/s0167-8140(21)08372-9 ◽

2021 ◽

Vol 161 ◽

pp. S1638-S1639

Author(s):

M. Aquilano ◽

G. Salvatore ◽

M. Loi ◽

D. Greto ◽

E. Scoccimarro ◽

...

Keyword(s):

Soft Tissue ◽

Soft Tissue Sarcoma ◽

Radiosensitizing Effect ◽

Human Soft Tissue ◽

Sarcoma Cells

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Comparative Genomics ofEscherichia coliIsolated from Skin and Soft Tissue and Other Extraintestinal Infections

mBio ◽

10.1128/mbio.01070-17 ◽

2017 ◽

Vol 8 (4) ◽

Cited By ~ 12

Author(s):

Amit Ranjan ◽

Sabiha Shaik ◽

Nishant Nandanwar ◽

Arif Hussain ◽

Sumeet K. Tiwari ◽

...

Keyword(s):

Escherichia Coli ◽

Comparative Genomics ◽

Soft Tissue ◽

Multidrug Resistant ◽

Primary Concern ◽

Beta Lactamase ◽

Soft Tissue Infections ◽

E Coli ◽

Recent Emergence ◽

Tract Infections

ABSTRACTEscherichia coli, an intestinal Gram-negative bacterium, has been shown to be associated with a variety of diseases in addition to intestinal infections, such as urinary tract infections (UTIs), meningitis in neonates, septicemia, skin and soft tissue infections (SSTIs), and colisepticemia. Thus, for nonintestinal infections, it is categorized as extraintestinal pathogenicE. coli(ExPEC). It is also an opportunistic pathogen, causing cross infections, notably as an agent of zoonotic diseases. However, comparative genomic data providing functional and genetic coordinates for ExPEC strains associated with these different types of infections have not proven conclusive. In the study reported here, ExPECE. coliisolated from SSTIs was characterized, including virulence and drug resistance profiles, and compared with isolates from patients suffering either pyelonephritis or septicemia. Results revealed that the majority of the isolates belonged to two pathogenic phylogroups, B2 and D. Approximately 67% of the isolates were multidrug resistant (MDR), with 85% producing extended-spectrum beta-lactamase (ESBL) and 6% producing metallo-beta-lactamase (MBL). TheblaCTX-M-15genotype was observed in at least 70% of theE. coliisolates in each category, conferring resistance to an extended range of beta-lactam antibiotics. Whole-genome sequencing and comparative genomics of the ExPEC isolates revealed that two of the four isolates from SSTIs, NA633 and NA643, belong to pandemic sequence type ST131, whereas functional characteristics of three of the ExPEC pathotypes revealed that they had equal capabilities to form biofilm and were resistant to human serum. Overall, the isolates from a variety of ExPEC infections demonstrated similar resistomes and virulomes and did not display any disease-specific functional or genetic coordinates.IMPORTANCEInfections caused by extraintestinal pathogenicE. coli(ExPEC) are of global concern as they result in significant costs to health care facilities management. The recent emergence of a multidrug-resistant pandemic clone,Escherichia coliST131, is of primary concern as a global threat. In developing countries, such as India, skin and soft tissue infections (SSTIs) associated withE. coliare marginally addressed. In this study, we employed both genomic analysis and phenotypic assays to determine relationships, if any, among the ExPEC pathotypes. Similarity between antibiotic resistance and virulence profiles was observed, ST131 isolates from SSTIs were reported, and genomic similarities among strains isolated from different disease conditions were detected. This study provides functional molecular infection epidemiology insight into SSTI-associatedE. colicompared with ExPEC pathotypes.

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Design and Production of Two-Piece Thyroid-Neck Phantom by the Concurrent Use of Epoxy Resin and Poly(Methyl Methacrylate) Soft Tissue Equivalent Materials

Journal of Biomedical Physics and Engineering ◽

10.31661/jbpe.v8i2.952 ◽

2018 ◽

Vol 8 (2) ◽

Author(s):

M Karimi ◽

H Mostaghimi ◽

S. F Shams ◽

A R Mehdizadeh

Keyword(s):

Soft Tissue ◽

Methyl Methacrylate ◽

Epoxy Resin ◽

Attenuation Coefficients ◽

Poly Methyl Methacrylate ◽

Tissue Equivalent ◽

Concurrent Use ◽

Human Soft Tissue ◽

Mass Attenuation Coefficients ◽

Mass Attenuation

The aim of this report is to present a new two-piece thyroid-neck phantom produced by the concurrent use of epoxy resin and poly(methyl methacrylate) (PMMA: plexiglass) soft tissue equivalent materials. Accordingly, mass attenuation coefficients of the epoxy resin and the plexiglass compounds were obtained from simulation (NIST XCOM 3.1) and measurements (practical dosimetry) and compared to those related to human soft tissue (ICRU 44). The thyroid-neck phantom and thyroid gland dimensions were derived from scientific references and the atlas of human anatomy, respectively. The thyroid phantom was designed by CATIA V5R16 software and produced by the epoxy resin compound by three-dimensional printer. Other organs were designed by ProNest software and made by the plexiglass sheets by CNC laser cutting machine. The mass attenuation coefficients for the epoxy resin (50 keV- 20 MeV) and the plexiglass (0-20 MeV) were comparable to human soft tissue (ICRU 44), all with standard relative deviation beneath 5%. In addition, the SPECT images indicated the similarity between human thyroid tissue and its phantom. In conclusion, this study proves the feasibility and reliability of epoxy resin application in the production of two-piece thyroid-neck phantom. This phantom can be applied in the calibration of gamma camera systems, dosimetry and gamma spectrometry in the nuclear medicine field.

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Estimation of Anisotropic Material Properties of Soft Tissue by MRI of Ultrasound-Induced Shear Waves

Journal of Biomechanical Engineering ◽

10.1115/1.4046127 ◽

2020 ◽

Vol 142 (3) ◽

Author(s):

Charlotte A. Guertler ◽

Ruth J. Okamoto ◽

Jake A. Ireland ◽

Christopher P. Pacia ◽

Joel R. Garbow ◽

...

Keyword(s):

Soft Tissue ◽

Focused Ultrasound ◽

Ex Vivo ◽

Tissue Sample ◽

Shear Waves ◽

Chicken Breast ◽

Fiber Direction ◽

Fibrous Materials ◽

Phase Gradient ◽

Anisotropic Mechanical Properties

Abstract This paper describes a new method for estimating anisotropic mechanical properties of fibrous soft tissue by imaging shear waves induced by focused ultrasound (FUS) and analyzing their direction-dependent speeds. Fibrous materials with a single, dominant fiber direction may exhibit anisotropy in both shear and tensile moduli, reflecting differences in the response of the material when loads are applied in different directions. The speeds of shear waves in such materials depend on the propagation and polarization directions of the waves relative to the dominant fiber direction. In this study, shear waves were induced in muscle tissue (chicken breast) ex vivo by harmonically oscillating the amplitude of an ultrasound beam focused in a cylindrical tissue sample. The orientation of the fiber direction relative to the excitation direction was varied by rotating the sample. Magnetic resonance elastography (MRE) was used to visualize and measure the full 3D displacement field due to the ultrasound-induced shear waves. The phase gradient (PG) of radially propagating “slow” and “fast” shear waves provided local estimates of their respective wave speeds and directions. The equations for the speeds of these waves in an incompressible, transversely isotropic (TI), linear elastic material were fitted to measurements to estimate the shear and tensile moduli of the material. The combination of focused ultrasound and MR imaging allows noninvasive, but comprehensive, characterization of anisotropic soft tissue.

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