Molecular Identification of Anion Exchange Protein 3 in Pacific White Shrimp (Litopenaeus vannamei): mRNA Profiles for Tissues, Ontogeny, Molting, and Ovarian Development and Its Potential Role in Stress-Induced Gill Damage

Bicarbonate (HCO3–) transport mechanisms play an essential role in the acid-base homeostasis of aquatic animals, and anion exchange protein 3 (AE3) is a membrane transport protein that exchanges Cl–/HCO3– across the cell membrane to regulate the intracellular pH. In this study, the full-length cDNA of AE3 (Lv-AE3) was obtained from the Pacific white shrimp (Litopenaeus vannamei). The Lv-AE3 cDNA is 4,943 bp in length, contains an open reading frame of 2,850 bp, coding for a protein of 949 amino acids with 12 transmembrane domains. Lv-AE3 shows high sequence homology with other AE3 at the protein level. Lv-AE3 mRNA was ubiquitously detected in all tissues selected, with the highest expression level in the gill, followed by the ovary, eyestalk and brain. By in situ hybridization, Lv-AE3-positive cells were shown predominant localization in the secondary gill filaments. The expression levels of Lv-AE3 were further investigated during the essential life processes of shrimp, including ontogeny, molting, and ovarian development. In this case, the spatiotemporal expression profiles of Lv-AE3 in L. vannamei were highly correlated with the activities of water and ion absorption; for example, increased mRNA levels were present after hatching, during embryonic development, after ecdysis during the molt cycle, and in the stage IV ovary during gonadal development. After low/high pH and low/high salinity challenges, the transcript levels of Lv-AE3 were reduced in the gill, while the cell apoptosis rate increased. In addition, knockdown of Lv-AE3 mRNA expression induced cell apoptosis in the gill, indicating a potential link between Lv-AE3 and gill damage. Altogether, this study thoroughly investigated the relationship between the mRNA expression profiles of Lv-AE3 and multiple developmental and physiological processes in L. vannamei, and it may benefit the protection of crustaceans from fluctuated aquatic environments.

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Effects of gonadotropin-releasing hormones and dopamine on ovarian maturation in the Pacific white shrimp, Litopenaeus vannamei, and their presence in the ovary during ovarian development

Aquaculture ◽

10.1016/j.aquaculture.2013.10.036 ◽

2014 ◽

Vol 420-421 ◽

pp. 79-88 ◽

Cited By ~ 14

Author(s):

Yotsawan Tinikul ◽

Jaruwan Poljaroen ◽

Ruchanok Tinikul ◽

Panat Anuracpreeda ◽

Charoonroj Chotwiwatthanakun ◽

...

Keyword(s):

Litopenaeus Vannamei ◽

Ovarian Development ◽

Pacific White Shrimp ◽

White Shrimp ◽

Ovarian Maturation ◽

The Pacific

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Heat Shock Protein 40 (HSP40) in Pacific White Shrimp (Litopenaeus vannamei): Molecular Cloning, Tissue Distribution and Ontogeny, Response to Temperature, Acidity/Alkalinity and Salinity Stresses, and Potential Role in Ovarian Development

Frontiers in Physiology ◽

10.3389/fphys.2018.01784 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 13

Author(s):

Ting Chen ◽

Tiehao Lin ◽

Hongmei Li ◽

Ting Lu ◽

Jiaxi Li ◽

...

Keyword(s):

Heat Shock ◽

Heat Shock Protein ◽

Tissue Distribution ◽

Molecular Cloning ◽

Litopenaeus Vannamei ◽

Potential Role ◽

Ovarian Development ◽

Pacific White Shrimp ◽

White Shrimp ◽

Response To Temperature

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Characterization and Expression Analysis of Insulin Growth Factor Binding Proteins (IGFBPs) in Pacific White Shrimp Litopenaeus vannamei

International Journal of Molecular Sciences ◽

10.3390/ijms22031056 ◽

2021 ◽

Vol 22 (3) ◽

pp. 1056

Author(s):

Ying Pang ◽

Xiaojun Zhang ◽

Jianbo Yuan ◽

Xiaoxi Zhang ◽

Jianhai Xiang ◽

...

Keyword(s):

Growth Factor ◽

Litopenaeus Vannamei ◽

Binding Proteins ◽

Developmental Stages ◽

Expression Profiles ◽

Serine Proteinase ◽

Pacific White Shrimp ◽

White Shrimp ◽

Factor Binding ◽

Insulin Growth Factor

The insulin signaling (IIS) pathway plays an important role in the metabolism, growth, development, reproduction, and longevity of an organism. As a key member of the IIS pathway, insulin-like growth factor binding proteins (IGFBPs) are widely distributed a family in invertebrates and vertebrates that are critical in various aspects of physiology. As an important mariculture species, the growth of Pacific white shrimp, Litopenaeus vannamei, is one of the most concerning characteristics in this area of study. In this study, we identified three IGFBP genes in the genome of L. vannamei and analyzed their gene structures, phylogenetics, and expression profiles. LvIGFBP1 was found to contain three domains (the insulin growth factor binding (IB) domain, the Kazal-type serine proteinase inhibitor (Kazal) domain, and the immunoglobulin C-2 (IGc2) domain), while LvIGFBP2 and LvIGFBP3 only contained a single IB domain. LvIGFBP1 exhibited high expression in most tissues and different developmental stages, while LvIGFBP2 and LvIGFBP3 were only slightly expressed in hemocytes. The RNA interference of LvIGFBP1 resulted in a significantly smaller increment of body weight than that of control groups. These results will improve our understanding of the conservative structure and function of IGFBPs and show potential applications for the growth of shrimp.

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Alterations in the levels and distribution of octopamine in the central nervous system and ovary of the Pacific white shrimp, Litopenaeus vannamei, and its possible role in ovarian development

General and Comparative Endocrinology ◽

10.1016/j.ygcen.2014.09.018 ◽

2015 ◽

Vol 210 ◽

pp. 12-22 ◽

Cited By ~ 12

Author(s):

Yotsawan Tinikul ◽

Jaruwan Poljaroen ◽

Ruchanok Tinikul ◽

Charoonroj Chotwiwatthanakun ◽

Panat Anuracpreeda ◽

...

Keyword(s):

Central Nervous System ◽

Nervous System ◽

Litopenaeus Vannamei ◽

Ovarian Development ◽

Pacific White Shrimp ◽

White Shrimp ◽

The Pacific ◽

The Central Nervous System

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Glycogen Synthase Kinase 3 Gene Is Important in Growth and Molting of the Pacific White Shrimp Litopenaeus vannamei

Frontiers in Marine Science ◽

10.3389/fmars.2021.681966 ◽

2021 ◽

Vol 8 ◽

Author(s):

Ying Pang ◽

Xiaojun Zhang ◽

Jianbo Yuan ◽

Xiaoxi Zhang ◽

Manwen Su ◽

...

Keyword(s):

Protein Kinase ◽

Litopenaeus Vannamei ◽

Developmental Stages ◽

Glycogen Synthase ◽

Expression Profiles ◽

Pacific White Shrimp ◽

Glycogen Synthase Kinase ◽

White Shrimp ◽

Glycogen Synthase Kinase 3 ◽

The Pacific

Glycogen synthase kinase 3 (GSK3) is a vital multifunctional molecule that is widely distributed in invertebrates and vertebrates. GSK3 is a highly conserved serine/threonine (Ser/Thr) protein kinase, which plays an important role in insulin, Wnt, and various signaling pathways. In this study, a GSK3 gene were identified in the genome of the Pacific white shrimp, Litopenaeus vannamei, and analyzed its gene structure, phylogeny, and expression profiles. The deduced LvGSK3 protein contains a highly conserved Ser/Thr protein kinase catalytic (S_TKc) domain, the LvGSK3 gene exhibited high expression in different early developmental stages, most adult tissues, and premolting stages. RNA interference of LvGSK3 significantly retarded the increment of body weight and affected the expressions of molting-related genes compared with control groups. These results will improve our understanding of the conserved structure and functions of the LvGSK3 gene and show potential applications of shrimp growth.

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