MrMYB6 From Chinese Bayberry (Myrica rubra) Negatively Regulates Anthocyanin and Proanthocyanidin Accumulation

Anthocyanins and proanthocyanidins (PAs) are important flavonoids in Chinese bayberry (Morella rubra), which functions in fruit color and exhibits multiple health promoting and disease-preventing effects. To investigate the regulation of their biosynthesis in Chinese bayberries, we isolated and identified a subgroup 4 MYB transcription factor (TF), MrMYB6, and found MrMYB6 shared similar repressor domains with other MYB co-repressors of anthocyanin and PA biosynthesis after sequence analysis. Gene expression results revealed the transcripts of MrMYB6 were negatively correlated with the anthocyanin and insoluble PA contents and also with the gene expressions involved in anthocyanin biosynthesis and PA specific genes such as MrLAR and MrANR during the late ripening stages of bayberries. In addition, overexpression of MrMYB6 in tobacco inhibited the transcript levels of NtCHI, NtLAR, and NtANR2, resulting into a decline in the levels of anthocyanins and PAs in tobacco flowers. We further found that MrMYB6 interacted with MrbHLH1 and MrWD40-1 to form functional complexes that acted to directly repress the promoter activities of the PA-specific gene MrLAR and MrANR and the anthocyanin-specific gene MrANS and MrUFGT. Taken together, our results suggested that MrMYB6 might negatively regulate anthocyanin and PA accumulation in Chinese bayberry.

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Functional R2R3-MYB Transcription Factor NsMYB1, Regulating Anthocyanin Biosynthesis, Was Relative To The Fruit Color Differentiation in Nitraria Sibirica Pall

10.21203/rs.3.rs-1163034/v1 ◽

2021 ◽

Author(s):

Xuemei Bao ◽

Yuan Zong ◽

Na Hu ◽

Shiming Li ◽

Baolong Liu ◽

...

Keyword(s):

Transcription Factor ◽

Chemical Analysis ◽

Anthocyanin Biosynthesis ◽

Fruit Color ◽

Tibet Plateau ◽

Myb Transcription Factor ◽

Structural Genes ◽

Transcript Factor ◽

Nitraria Sibirica ◽

R2r3 Myb

Abstract Background Nitraria sibirica Pall. is an economic plant with two kinds of fruit color, widely spreads in the Qinghai Tibet Plateau. The chemical analysis and pharmacological evaluation had been carried out for several tens of years, the mechanism behind the fruit color differentiation is still unclear. Results In this manuscript, the chemical analysis of the extractions showed that the chemical composition of fruit color was anthocyanin, and two kind of Nitraria sibirica Pall. were caused by the content differentiation with the same anthocyanin kinds. Cya-nidin-3-[2ʹ’-(6ʹ’’-coumaroyl)-glucosyl]-glucoside (C3G) was the major anthocyanin. Transcriptome analysis and the qRT-PCR revealed that the structural genes relative to anthocyanin biosynthesis except CHS, F3’5’H and ANS were up-regulated in BF compared with RF, which indicated that transcript factor should be the reason for the expression difference of the structure genes. In the unigenes of the transcript factor MYB and bHLH, relative to anthocyanin, only NsMYB1 (Clus-ter-8422.10600), was high-expression and up-expression in the BF. NsMYB1 encoded the same length protein with four amino acid differences in the RF and BF, and both contained the intact DNA, HTH-MYB and SANT domains. NsMYB1 was close to the AtMYB114, AtMYB113 and AtPAP1, regulating anthocyanin biosynthesis, in phylogenetic relationship. Both NsMYB1r and NsMYB1b could promote the transcript of the structural genes, and induced the anthocyanin accumulation in all tissues of transgenic tobacco. The insertion of ‘TATA’ in the promoter of NsMYB1r gave one more promoter region, and was the reason for higher transcripts in black fruit possibly. Conclusions NsMYB1 was a functional R2R3-MYB transcription factor, regulated the anthocyanin biosynthesis, and leaded to the fruit color differentiation in Nitraria sibirica Pall.

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Coordinated regulation of anthocyanin biosynthesis in Chinese bayberry (Myrica rubra) fruit by a R2R3 MYB transcription factor

Planta ◽

10.1007/s00425-009-1095-z ◽

2010 ◽

Vol 231 (4) ◽

pp. 887-899 ◽

Cited By ~ 142

Author(s):

Shan-Shan Niu ◽

Chang-Jie Xu ◽

Wang-Shu Zhang ◽

Bo Zhang ◽

Xian Li ◽

...

Keyword(s):

Transcription Factor ◽

Anthocyanin Biosynthesis ◽

Myb Transcription Factor ◽

Chinese Bayberry ◽

Myrica Rubra ◽

Coordinated Regulation ◽

R2r3 Myb

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Conserved and non-conserved functions of the rice homologs of the Arabidopsis trichome initiation-regulating MBW complex proteins

BMC Plant Biology ◽

10.1186/s12870-021-03035-0 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Kaijie Zheng ◽

Xutong Wang ◽

Yating Wang ◽

Shucai Wang

Keyword(s):

Transcription Factor ◽

Anthocyanin Biosynthesis ◽

Transcriptional Activator ◽

Myb Transcription Factor ◽

Bhlh Transcription Factor ◽

Seed Color ◽

Transcription Activator ◽

Root Hair Formation ◽

Protoplast Transfection ◽

Mbw Complex

Abstract Background Trichome initiation in Arabidopsis is regulated by a MYB-bHLH-WD40 (MBW) transcriptional activator complex formed by the R2R3 MYB transcription factor GLABRA1 (GL1), MYB23 or MYB82, the bHLH transcription factor GLABRA3 (GL3), ENHANCER OF GLABRA3 (EGL3) or TRANSPARENT TESTA8 (TT8), and the WD40-repeat protein TRANSPARENT TESTA GLABRA1 (TTG1). However, the functions of the rice homologs of the MBW complex proteins remained uncharacterized. Results Based on amino acid sequence identity and similarity, and protein interaction prediction, we identified OsGL1s, OsGL3s and OsTTG1s as rice homologs of the MBW complex proteins. By using protoplast transfection, we show that OsGL1D, OsGL1E, OsGL3B and OsTTG1A were predominantly localized in the nucleus, OsGL3B functions as a transcriptional activator and is able to interact with GL1 and TTG1. By using yeast two-hybrid and protoplast transfection assays, we show that OsGL3B is able to interact with OsGL1E and OsTTG1A, and OsGL1E and OsTTG1A are also able to interact with GL3. On the other hand, we found that OsGL1D functions as a transcription activator, and it can interact with GL3 but not OsGL3B. Furthermore, our results show that expression of OsTTG1A in the ttg1 mutant restored the phenotypes including alternations in trichome and root hair formation, seed color, mucilage production and anthocyanin biosynthesis, indicating that OsTTG1A and TTG1 may have similar functions. Conclusion These results suggest that the rice homologs of the Arabidopsis MBW complex proteins are able to form MBW complexes, but may have conserved and non-conserved functions.

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Promoter G-quadruplexes and transcription factors cooperate to shape the cell type-specific transcriptome

Nature Communications ◽

10.1038/s41467-021-24198-2 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Sara Lago ◽

Matteo Nadai ◽

Filippo M. Cernilogar ◽

Maryam Kazerani ◽

Helena Domíniguez Moreno ◽

...

Keyword(s):

Transcription Factors ◽

Binding Sites ◽

Specific Gene ◽

Open Chromatin ◽

Rna Seq ◽

Transcript Levels ◽

Promoter Sequences ◽

G Quadruplex ◽

Cell Type Specific ◽

Folding State

AbstractCell identity is maintained by activation of cell-specific gene programs, regulated by epigenetic marks, transcription factors and chromatin organization. DNA G-quadruplex (G4)-folded regions in cells were reported to be associated with either increased or decreased transcriptional activity. By G4-ChIP-seq/RNA-seq analysis on liposarcoma cells we confirmed that G4s in promoters are invariably associated with high transcription levels in open chromatin. Comparing G4 presence, location and transcript levels in liposarcoma cells to available data on keratinocytes, we showed that the same promoter sequences of the same genes in the two cell lines had different G4-folding state: high transcript levels consistently associated with G4-folding. Transcription factors AP-1 and SP1, whose binding sites were the most significantly represented in G4-folded sequences, coimmunoprecipitated with their G4-folded promoters. Thus, G4s and their associated transcription factors cooperate to determine cell-specific transcriptional programs, making G4s to strongly emerge as new epigenetic regulators of the transcription machinery.

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Coculture with hiPS-derived intestinal cells enhanced human hepatocyte functions in a pneumatic-pressure-driven two-organ microphysiological system

Scientific Reports ◽

10.1038/s41598-021-84861-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Marie Shinohara ◽

Hiroshi Arakawa ◽

Yuuichi Oda ◽

Nobuaki Shiraki ◽

Shinji Sugiura ◽

...

Keyword(s):

Normal Cell ◽

Human Hepatocytes ◽

New Drugs ◽

Intestinal Cells ◽

Specific Gene ◽

Gene Expressions ◽

Normal Cells ◽

Pneumatic Pressure ◽

Pressure Driven

AbstractExamining intestine–liver interactions is important for achieving the desired physiological drug absorption and metabolism response in in vitro drug tests. Multi-organ microphysiological systems (MPSs) constitute promising tools for evaluating inter-organ interactions in vitro. For coculture on MPSs, normal cells are challenging to use because they require complex maintenance and careful handling. Herein, we demonstrated the potential of coculturing normal cells on MPSs in the evaluation of intestine–liver interactions. To this end, we cocultured human-induced pluripotent stem cell-derived intestinal cells and fresh human hepatocytes which were isolated from PXB mice with medium circulation in a pneumatic-pressure-driven MPS with pipette-friendly liquid-handling options. The cytochrome activity, albumin production, and liver-specific gene expressions in human hepatocytes freshly isolated from a PXB mouse were significantly upregulated via coculture with hiPS-intestinal cells. Our normal cell coculture shows the effects of the interactions between the intestine and liver that may occur in vivo. This study is the first to demonstrate the coculturing of hiPS-intestinal cells and fresh human hepatocytes on an MPS for examining pure inter-organ interactions. Normal-cell coculture using the multi-organ MPS could be pursued to explore unknown physiological mechanisms of inter-organ interactions in vitro and investigate the physiological response of new drugs.

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An R2R3-MYB transcription factor CmMYB21 represses anthocyanin biosynthesis in color fading petals of chrysanthemum

Scientia Horticulturae ◽

10.1016/j.scienta.2021.110674 ◽

2022 ◽

Vol 293 ◽

pp. 110674

Author(s):

Yiguang Wang ◽

Li-Jie Zhou ◽

Yuxi Wang ◽

Zhiqiang Geng ◽

Baoqing Ding ◽

...

Keyword(s):

Transcription Factor ◽

Anthocyanin Biosynthesis ◽

Myb Transcription Factor ◽

Color Fading ◽

R2r3 Myb

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Between-country inequalities in health lifestyles

International Journal of Comparative Sociology ◽

10.1177/00207152211041385 ◽

2021 ◽

pp. 002071522110413

Author(s):

Tom VanHeuvelen ◽

Jane S VanHeuvelen

Keyword(s):

Economic Development ◽

Health Behaviors ◽

European Social Survey ◽

Living Condition ◽

Social Survey ◽

Health Promoting Behaviors ◽

Health Lifestyles ◽

Health Promoting ◽

Multiple Health ◽

Representative Samples

How does engagement in multiple health behaviors consolidate into health promoting health lifestyles, and how does economic development provide a broadly shared living condition to enable participation in health promoting health lifestyles? To answer these questions, we harmonize information from the 2011 International Social Survey Programme and the 2014 European Social Survey to examine patterns of health lifestyles and subsequent associations with self-rated health in representative samples of 52 country-years nested in 35 countries, with repeated observations from 17 countries. We find individuals engage more frequently in health promoting behaviors in countries with higher levels of economic development. Moreover, we find a tighter connection between health lifestyles and health in countries with higher levels of economic development. Critically, we move health lifestyles research forward by testing the consequences of within country changes in economic development, finding that growth in economic development increases the engagement of health promoting health behaviors. Policy and theoretical implications are discussed.

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The role of MrbHLH1 and MrMYB1 in regulating anthocyanin biosynthetic genes in tobacco and Chinese bayberry (Myrica rubra) during anthocyanin biosynthesis

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-013-0361-8 ◽

2013 ◽

Vol 115 (3) ◽

pp. 285-298 ◽

Cited By ~ 34

Author(s):

Xiao-Fen Liu ◽

Xue-Ren Yin ◽

Andrew C. Allan ◽

Kui Lin-Wang ◽

Yan-Na Shi ◽

...

Keyword(s):

Anthocyanin Biosynthesis ◽

Biosynthetic Genes ◽

Chinese Bayberry ◽

Myrica Rubra ◽

Anthocyanin Biosynthetic Genes

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Dysregulation of MS risk genes and pathways at distinct stages of disease

Neurology Neuroimmunology & Neuroinflammation ◽

10.1212/nxi.0000000000000337 ◽

2017 ◽

Vol 4 (3) ◽

pp. e337 ◽

Cited By ~ 16

Author(s):

Sundararajan Srinivasan ◽

Marco Di Dario ◽

Alessandra Russo ◽

Ramesh Menon ◽

Elena Brini ◽

...

Keyword(s):

Gene Expression ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Gene List ◽

Literature Mining ◽

Specific Gene ◽

Healthy Population ◽

Gene Expressions ◽

Altered Expression ◽

Risk Genes

Objective:To perform systematic transcriptomic analysis of multiple sclerosis (MS) risk genes in peripheral blood mononuclear cells (PBMCs) of subjects with distinct MS stages and describe the pathways characterized by dysregulated gene expressions.Methods:We monitored gene expression levels in PBMCs from 3 independent cohorts for a total of 297 cases (including clinically isolated syndromes (CIS), relapsing-remitting MS, primary and secondary progressive MS) and 96 healthy controls by distinct microarray platforms and quantitative PCR. Differential expression and pathway analyses for distinct MS stages were defined and validated by literature mining.Results:Genes located in the vicinity of MS risk variants displayed altered expression in peripheral blood at distinct stages of MS compared with the healthy population. The frequency of dysregulation was significantly higher than expected in CIS and progressive forms of MS. Pathway analysis for each MS stage–specific gene list showed that dysregulated genes contributed to pathogenic processes with scientific evidence in MS.Conclusions:Systematic gene expression analysis in PBMCs highlighted selective dysregulation of MS susceptibility genes playing a role in novel and well-known pathogenic pathways.

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