scholarly journals Comparative Transcriptomics and Metabolomics Reveal an Intricate Priming Mechanism Involved in PGPR-Mediated Salt Tolerance in Tomato

2021 ◽  
Vol 12 ◽  
Author(s):  
Ifigeneia Mellidou ◽  
Aggeliki Ainalidou ◽  
Anastasia Papadopoulou ◽  
Kleopatra Leontidou ◽  
Savvas Genitsaris ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Inorganic Phosphorus ◽  
Stress Factors ◽  
Salt Adaptation ◽  
Photosynthetic Parameters ◽  
Abiotic Stress Factors ◽  
Plant Growth Promoting ◽  
Primed State ◽  
Stress Signals

Plant-associated beneficial strains inhabiting plants grown under harsh ecosystems can help them cope with abiotic stress factors by positively influencing plant physiology, development, and environmental adaptation. Previously, we isolated a potential plant growth promoting strain (AXSa06) identified as Pseudomonas oryzihabitans, possessing 1-aminocyclopropane-1-carboxylate deaminase activity, producing indole-3-acetic acid and siderophores, as well as solubilizing inorganic phosphorus. In this study, we aimed to further evaluate the effects of AXSa06 seed inoculation on the growth of tomato seedlings under excess salt (200 mM NaCl) by deciphering their transcriptomic and metabolomic profiles. Differences in transcript levels and metabolites following AXSa06 inoculation seem likely to have contributed to the observed difference in salt adaptation of inoculated plants. In particular, inoculations exerted a positive effect on plant growth and photosynthetic parameters, imposing plants to a primed state, at which they were able to respond more robustly to salt stress probably by efficiently activating antioxidant metabolism, by dampening stress signals, by detoxifying Na+, as well as by effectively assimilating carbon and nitrogen. The primed state of AXSa06-inoculated plants is supported by the increased leaf lipid peroxidation, ascorbate content, as well as the enhanced activities of antioxidant enzymes, prior to stress treatment. The identified signatory molecules of AXSa06-mediated salt tolerance included the amino acids aspartate, threonine, serine, and glutamate, as well as key genes related to ethylene or abscisic acid homeostasis and perception, and ion antiporters. Our findings represent a promising sustainable solution to improve agricultural production under the forthcoming climate change conditions.

Performance of plant growth-promoting bacterium of duckweed under different kinds of abiotic stress factors

2019 ◽  
Vol 19 ◽  
pp. 101146 ◽  
Author(s):  
Hidehiro Ishizawa ◽  
Minami Tada ◽  
Masashi Kuroda ◽  
Daisuke Inoue ◽  
Michihiko Ike
Keyword(s):  
Abiotic Stress ◽  
Plant Growth ◽  
Stress Factors ◽  
Abiotic Stress Factors ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Genome Insights into the Novel Species Jejubacter calystegiae, a Plant Growth-Promoting Bacterium in Saline Conditions

Diversity ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 24
Author(s):  
Ling Min Jiang ◽  
Yong Jae Lee ◽  
Ho Le Han ◽  
Myoung Hui Lee ◽  
Jae Cheol Jeong ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Novel Species ◽  
Endophytic Bacterium ◽  
Morning Glory ◽  
Plant Growth Promoting Bacteria ◽  
Illumina Hiseq ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Sequencing Platforms

Jejubacter calystegiae KSNA2T, a moderately halophilic, endophytic bacterium isolated from beach morning glory (Calystegia soldanella), was determined to be a novel species in a new genus in the family Enterobacteriaceae. To gain insights into the genetic basis of the salinity stress response of strain KSNA2T, we sequenced its genome using two complementary sequencing platforms (Illumina HiSeq and PacBio RSII). The genome contains a repertoire of metabolic pathways, such as those for nitrogen, phosphorus, and some amino acid metabolism pathways. Functional annotation of the KSNA2T genome revealed several genes involved in salt tolerance pathways, such as those encoding sodium transporters, potassium transporters, and osmoprotectant enzymes. Plant growth-promoting bacteria-based experiments indicated that strain KSNA2T promotes the germination of vegetable seeds in saline conditions. Overall, the genetic and biological analyses of strain KSNA2T provide valuable insights into bacteria-mediated salt tolerance in agriculture.

First insights into salt tolerance improvement of Stevia by plant growth-promoting Streptomyces species

2019 ◽  
Vol 201 (9) ◽  
pp. 1295-1306 ◽  
Author(s):  
Sahar T. M. Tolba ◽  
Mohamed Ibrahim ◽  
Essam A. M. Amer ◽  
Doaa A. M. Ahmed
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Streptomyces Species ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Tolerance Improvement

Salt-tolerant and plant-growth-promoting bacteria isolated from high-yield paddy soil

2018 ◽  
Vol 64 (12) ◽  
pp. 968-978 ◽  
Author(s):  
Shiying Zhang ◽  
Cong Fan ◽  
Yongxia Wang ◽  
Yunsheng Xia ◽  
Wei Xiao ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Paddy Soil ◽  
Effective Strain ◽  
High Yield ◽  
Saline Stress ◽  
Plant Growth Promoting Bacteria ◽  
Salt Tolerant ◽  
Plant Growth Promoting ◽  
Growth Promoting

Growth and productivity of rice is negatively affected by soil salinity. However, some salt-tolerant bacteria improve the health of plants under saline stress. In this study, 305 bacteria were isolated from paddy soil in Taoyuan, China. Among these, 162 strains were tested for salt-tolerance; 67.3%, 28.4%, and 9.3% of the strains could grow in media with NaCl concentrations of 50, 100, and 150 g/L, respectively. The phylogenic analysis of 74 of these 162 strains indicates that these bacteria belong to Bacillales (72%), Actinomycetales (22%), Rhizobiales (1%), and Oceanospirillales (4%). Among 162 strains, 30 salt-tolerant strains were screened for their plant-growth-promoting activities under axenic conditions at 3, 6, 9, and 12 g/L NaCl; 43%–97% of the strains could improve rice germination energy or germination capacity, while 63%–87% of the strains could increase shoot and root lengths. Among various plant-growth-promoting bacteria, TY0307 was the most effective strain for promoting the growth of rice, even at high salt stress. Its promotor effects were associated with its production of 1-aminocyclopropane-1-carboxycarboxylate deaminase, indole acetic acid, and siderophores; induction of proline accumulation; and reduction of the salt-induced malondialdehyde content. These results suggest that several strains isolated from paddy soil could improve rice salt tolerance and may be used in the development of biofertilizer.

scholarly journals Effect of Salt-Tolerant Bacterial Inoculations on Rice Seedlings Differing in Salt-Tolerance under Saline Soil Conditions

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1030 ◽  
Author(s):  
Rakiba Shultana ◽  
Ali Tan Kee Zuan ◽  
Mohd Rafii Yusop ◽  
Halimi Mohd Saud ◽  
Arolu Fatai Ayanda
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Water Content ◽  
Electrolyte Leakage ◽  
Relative Water Content ◽  
Rice Variety ◽  
Salt Tolerant ◽  
Relative Water ◽  
Plant Growth Promoting ◽  
Growth Promoting

Salt-tolerant plant growth-promoting rhizobacteria (PGPR) could be an alternative to alleviate salinity problems in rice plants grown in the coastal areas. This study was conducted to isolate and characterize salt-tolerant PGPR and observe their effects on the physiological and biochemical properties of rice plants grown under non-saline and saline glasshouse conditions. Three strains were selected based on their salt-tolerance and plant growth-promoting properties under in vitro saline conditions. These strains were identified as Bacillus tequilensis (UPMRB9), Bacillus aryabhattai (UPMRE6), and Providencia stuartii (UPMRG1) using a 16S rRNA technique. The selected strains were inoculated to three different rice varieties, namely BRRI dhan67 (salt-tolerant), Putra-1 (moderate salt-tolerant), and MR297 (salt-susceptible) under glasshouse conditions. Results showed that the MR297 rice variety inoculated with UPMRB9 produced the highest total chlorophyll content, with an increment of 28%, and lowest electrolyte leakage of 92%. The Putra-1 rice variety also showed a 156% total dry matter increase with the inoculation of this bacterial strain. The highest increase of relative water content and reduction of Na/K ratio were found upon inoculation of UPMRE6 and UPMRB9, respectively. The biggest significant effects of these bacterial inoculations were on relative water content, electrolyte leakage, and the Na/K ratio of the BRRI dhan67 rice variety under saline conditions, suggesting a synergistic effect on the mechanisms of plant salt-tolerance. This study has shown that the application of locally-isolated salt-tolerant PGPR strains could be an effective long-term and sustainable solution for rice cultivation in the coastal areas, which are affected by global climate change.

scholarly journals Plant growth promoting bacteria as an alternative strategy for salt tolerance in plants: A review

2018 ◽  
Vol 209 ◽  
pp. 21-32 ◽  
Author(s):  
Muhammad Numan ◽  
Samina Bashir ◽  
Yasmin Khan ◽  
Roqayya Mumtaz ◽  
Zabta Khan Shinwari ◽  
...  
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Alternative Strategy ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

scholarly journals Volatile Organic Compounds of the Plant Growth-Promoting Rhizobacteria JZ-GX1 Enhanced the Tolerance of Robinia pseudoacacia to Salt Stress

2021 ◽  
Vol 12 ◽  
Author(s):  
Pu-Sheng Li ◽  
Wei-Liang Kong ◽  
Xiao-Qin Wu ◽  
Yu Zhang
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Plant Growth ◽  
Lateral Root ◽  
Robinia Pseudoacacia ◽  
Plant Growth Promoting Rhizobacteria ◽  
Stress Conditions ◽  
Volatile Organic ◽  
Plant Growth Promoting ◽  
Growth Promoting

Salt stress is one of the major abiotic stresses that affects plant growth and development. The use of plant growth-promoting rhizobacteria to mitigcate salt stress damage in plants is an important way to promote crop growth under salt stress conditions. Rahnella aquatilis JZ-GX1 is a plant growth-promoting rhizobacterial strain, but it is not clear whether it can improve the salt tolerance of plants, and in particular, the role of volatile substances in plant salt tolerance is unknown. We investigated the effects of volatile organic compounds (VOCs) from JZ-GX1 on the growth performance, osmotic substances, ionic balance and antioxidant enzyme activities of acacia seedlings treated with 0 and 100mm NaCl and explored the VOCs associated with the JZ-GX1 strain. The results showed that compared to untreated seedlings, seedlings exposed to plant growth-promoting rhizobacterium JZ-GX1 via direct contact with plant roots under salt stress conditions exhibited increases in fresh weight, lateral root number and primary root length equal to approximately 155.1, 95.4, and 71.3%, respectively. Robinia pseudoacacia seedlings exposed to VOCs of the JZ-GX1 strain showed increases in biomass, soil and plant analyser development values and lateral root numbers equal to 132.1, 101.6, and 166.7%, respectively. Additionally, decreases in malondialdehyde, superoxide anion (O2−) and hydrogen peroxide (H2O2) contents and increases in proline contents and superoxide dismutase, peroxidase and glutathione reductase activities were observed in acacia leaves. Importantly, the sodium-potassium ratios in the roots, stems, and leaves of acacia exposed to VOCs of the JZ-GX1 strain were significantly lower than those in the control samples, and this change in ion homeostasis was consistent with the upregulated expression of the (Na+, K+)/H+ reverse cotransporter RpNHX1 in plant roots. Through GC-MS and creatine chromatography, we also found that 2,3-butanediol in the volatile gases of the JZ-GX1 strain was one of the important signaling substances for improving the salt tolerance of plants. The results showed that R. aquatilis JZ-GX1 can promote the growth and yield of R. pseudoacacia under normal and salt stress conditions. JZ-GX1 VOCs have good potential as protectants for improving the salt tolerance of plants, opening a window of opportunity for their application in salinized soils.

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