scholarly journals First Isolation and Molecular Characterization of blaCTX-M-121-Producing Escherichia coli O157:H7 From Cattle in Xinjiang, China

2021 ◽  
Vol 8 ◽  
Author(s):  
Zhanqiang Su ◽  
Panpan Tong ◽  
Ling Zhang ◽  
Mengmeng Zhang ◽  
Dong Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogen ◽  
Virulence Gene ◽  
Multidrug Resistant ◽  
Escherichia Coli O157 ◽  
Bacterial Strains ◽  
E Coli ◽  
Uremic Syndrome ◽  
Cattle Farms

The bovine Escherichia coli O157:H7 is a major foodborne pathogen causing severe bloody diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome in humans. Cattle are recognized major reservoir and source of E. coli O157:H7. We investigated the antibiotic resistance, molecular profiles, and intrinsic relationship between 21 isolates of E. coli O157:H7 from cattle farms and slaughtering houses in Xinjiang. Using pulsed-field gel electrophoresis (PFGE) molecular typing, two types of PFGE were revealed through cluster analysis, including clusters I and II, with 66 and 100% similarity of PFGE spectra between 21 isolates. We also detected that 18 isolates (86%) carried at least one virulence gene, 16 isolates (76%) carried the eae gene, and 7 (33%) carried the stx1 + stx2 + eae + hly + tccp genes. Eighteen isolates were susceptible to antibiotics. Three isolates were resistant to antibiotics, and two were multidrug resistant. One of the two multidrug-resistant isolates detectably carried the blaCTX−M−121 gene. This is the first finding of the blaCTX−M−121 gene detected in E. coli O157:H7 isolated from cattle in Xinjiang. The blaCTX−M−121 gene is transferable between the bacterial strains via plasmid transmission. The results indicated that E. coli O157:H7 may have undergone clonal propagation in cattle population and cross-regional transmission in Xinjiang, China.

scholarly journals First Isolation and Molecular Characterization of bla CTX-M-121 -producing Escherichia coli O157:H7 Strain Y4-A109 from Cattle in China

2020 ◽  
Author(s):  
Zhanqiang Su ◽  
Panpan Tong(Former Corresponding Author) ◽  
Ling Zhang ◽  
Mengmeng Zhang ◽  
Dong Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Horizontal Transmission ◽  
Virulence Gene ◽  
Multi Drug Resistance ◽  
Escherichia Coli O157 ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Cattle Farms

Abstract Background: To study the antibiotic resistance, the molecular epidemiology of bovine Escherichia coli ( E.coli ) O157:H7, and exploring the intrinsic relationship among different isolates, we have collected 27 bovine E. coli O157:H7 strains in Xinjiang from 2012 to 2017 and evaluated virulence genes, antibiotic resistance, and pulsed-field gel electrophoresis (PFGE) molecular typing. Results: Of all the 27 bovine E. coli O157:H7 strains analyzed, 21 strains contained at least one virulence gene, 19 strains carried eae gene (70.4%) and 8 of them carrying stx1 + stx2 + eae + hly + tccP . Most strains were sensitive to all the antibiotics tested. However, 4 of which were antibiotic-resistant, and 2 of which possessed multi-drug resistance, including one ESBL-producing strain. This is the first report of the bla CTX-M-121 gene in bovine E. coli O157:H7. Moreover, the bla CTX-M-121 gene can be transmitted horizontally through plasmid between strains. The similarity of PFGE spectra of 27 strains was between 65.8% and 100%. Two types of PFGE were obtained through cluster analysis, including clusters Ⅰ and Ⅱ. Conclusions: E. coli O157:H7 may have undergone clonal propagation in cattle farms as well as cross-regional transmission and horizontal transmission in different regions in Xinjiang China.

scholarly journals First Isolation and Molecular Characterization of blaCTX-M-121-producting Escherichia coli O157:H7 from China Cattle

2019 ◽  
Author(s):  
Zhanqiang Su ◽  
Panpan Tong ◽  
Ling Zhang ◽  
Mengmeng Zhang ◽  
Dong Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Clonal Propagation ◽  
Horizontal Transmission ◽  
Virulence Gene ◽  
Multi Drug Resistance ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Cattle Farms

Abstract Background: To study antibiotic resistance and the molecular epidemiology of bovine Escherichia coli (E.coli) O157:H7 and to explore the intrinsic relationship among different isolates, we collected 27 strains of bovine E. coli O157:H7 in Xinjiang from 2012 to 2017 and assessed virulence genes, antibiotic resistance and pulsed-field gel electrophoresis (PFGE) molecular typing. Results: In this study, 21 strains carried at least one virulence gene, and 19 strains carried eae gene (70.4%) including 8 carrying stx1+stx2+eae +hly+tccP. Most strains were sensitive to all antibiotics tested, 4 strains were bacteria-resistant, and 2 strains possessed multi-drug resistance, including one ESBL-producing strain. This is the first report of a blaCTX-M-121 gene in bovine E. coli O157:H7. Moreover, blaCTX-M-121 gene can be transmitted horizontally by plasmid between strains. The PFGE spectral similarity of the 27 strains was between 65.8% and 100%. Two PFGE types including clusters Ⅰ and Ⅱ were obtained through cluster analysis. Conclusions: E. coli O157:H7 may have undergone clonal propagation in cattle farms as well as cross-regional transmission. A horizontal transmission path with E. coli O157:H7 appeared in different areas.

scholarly journals Molecular Characterization of Plasmid-Mediated Non-O157 Verotoxigenic Escherichia coli Isolated from Infants and Children with Diarrhea

2020 ◽  
Vol 17 (3) ◽  
pp. 0710
Author(s):  
Md Fazlul Karim Khan ◽  
Shah Samiur Rashid
Keyword(s):  
Escherichia Coli ◽  
Virulence Gene ◽  
Multidrug Resistant ◽  
Plasmid Curing ◽  
Health Issues ◽  
E Coli ◽  
Disk Diffusion Assay ◽  
Microbiological Techniques ◽  
Polymerase Chain

A significant increase in the incidence of non-O157 verotoxigenic Escherichia coli (VTEC) infections have become a serious health issues, and this situation is worsening due to the dissemination of plasmid mediated multidrug-resistant microorganisms worldwide. This study aims to investigate the presence of plasmid-mediated verotoxin gene in non-O157 E. coli. Standard microbiological techniques identified a total of 137 E. coli isolates. The plasmid was detected by Perfectprep Plasmid Mini preparation kit. These isolates were subjected to disk diffusion assay, and plasmid curing with ethidium bromide treatment. The plasmid containing isolates were subjected to a polymerase chain reaction (PCR) for investigating the presence of plasmid mediated verotoxin gene (VT1 and VT2) in non-O157 E. coli. Among the 137 E. coli isolates, 49 isolates were non-O157 E. coli while 29 (59.1%) isolates were verotoxin producing non-O157 serotypes and 26 non-O157 VTEC isolates possessed plasmids. Certain isolates harboured single sized plasmid while others had multiple plasmids with different size varied from 1.8kb to 7.6kb. A plasmid containing all (100%) the isolates was multidrug-resistant. Eight isolates changed their susceptibility patterns while three isolates were found to lose plasmid after post plasmid curing treatment and the rest of the isolates (15) remained constant. Different PCR sets characterized 3 plasmid-mediated verotoxins producing non-O157 E. coli. This current study demonstrated the occurrence of plasmid mediated verotoxin gene in non-O157 E. coli. To the best of our knowledge, this is the first report in the global literature on plasmid-mediated verotoxin gene in non-O157 E. coli. Timely diagnosis and surveillance of VTEC infections should prioritize to stop or slow down the virulence gene for dissemination by plasmid-mediated gene transfer amongst the same bacteria or other species.

A Comparison of the Survival in Feces and Water of Escherichia coli O157:H7 Grown under Laboratory Conditions or Obtained from Cattle Feces

2006 ◽  
Vol 69 (1) ◽  
pp. 6-11 ◽  
Author(s):  
L. SCOTT ◽  
P. McGEE ◽  
J. J. SHERIDAN ◽  
B. EARLEY ◽  
N. LEONARD
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogen ◽  
Hemorrhagic Colitis ◽  
Contaminated Water ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Cattle Feces ◽  
Oral Inoculation ◽  
Before And After ◽  
Uremic Syndrome

Escherichia coli O157:H7 is an important foodborne pathogen that can cause hemorrhagic colitis and hemolytic uremic syndrome. Cattle feces and fecally contaminated water are important in the transmission of this organism on the farm. In this study, the survival of E. coli O157:H7 in feces and water was compared following passage through the animal digestive tract or preparation in the laboratory. Feces were collected from steers before and after oral inoculation with a marked strain of E. coli O157:H7. Fecal samples collected before cattle inoculation were subsequently inoculated with the marked strain of E. coli O157:H7 prepared in the laboratory. Subsamples were taken from both animal and laboratory-inoculated feces to inoculate 5-liter volumes of water. E. coli O157:H7 in feces survived up to 97 days, and survival was not affected by the method used to prepare the inoculating strain. E. coli O157:H7 survived up to 109 days in water, and the bacteria collected from inoculated cattle were detected up to 10 weeks longer than the laboratory-prepared culture. This study suggests that pathogen survival in low-nutrient conditions may be enhanced by passage through the gastrointestinal tract.

scholarly journals Characterization of commensal Escherichia coli isolates from slaughtered sheep in Mexico

2021 ◽  
Vol 15 (11) ◽  
pp. 1755-1760
Author(s):  
Jorge Acosta-Dibarrat ◽  
Edgar Enriquez-Gómez ◽  
Martín Talavera-Rojas ◽  
Edgardo Soriano-Vargas ◽  
Armando Navarro ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Multidrug Resistant ◽  
Phylogenetic Group ◽  
Phylogenetic Groups ◽  
E Coli ◽  
New Information ◽  
Antimicrobial Resistance Genes ◽  
Uremic Syndrome

Introduction: Commensal Escherichia coli is defined as bacteria without known virulence factors that could be playing a specific role in some diseases; however, they could be responsible to disseminate antimicrobial resistance genes to other microorganisms. This study aimed to characterize the commensal E. coli isolates obtained from slaughtered sheep in the central region of Mexico. Methodology: Isolates were classified as commensal E. coli when distinctive genes related to diarrheagenic pathotypes (stx1, stx2, eae, bfp, LT, stp, ipaH, and aggR) were discarded by PCR. Identification of serotype, phylogenetic group, and antimicrobial resistance was also performed. Results: A total of 41 isolates were characterized. The phylogenetic groups found were B1 in 37 isolates (90.2%), A in 2 (4.8%), and 1 isolate (2.4%) for C and D groups. Serotypes associated with diarrhea in humans (O104:H2 and O154:NM) and hemolytic uremic syndrome (O8:NM) were detected. Thirty-three isolates (80%) were resistant to ceftazidime, 23 (56%), to tetracycline 8 (19.5%) to ampicillin, and 1 to amikacin. Six isolates (14.6%) were multidrug-resistant. Conclusions: This study provides new information about commensal E. coli in slaughtered sheep, high percentages of resistance to antibiotics, and different profiles of antimicrobial resistance were found, their dissemination constitute a risk factor towards the consuming population.

scholarly journals Prevalence and Virulence Gene Profiles of Escherichia coli O157 from Cattle Slaughtered in Buea, Cameroon

2020 ◽  
Author(s):  
Elvis Achondou Akomoneh ◽  
Seraphine Nkie Esemu ◽  
Achah Jerome Kfusi ◽  
Roland N. Ndip ◽  
Lucy M. Ndip
Keyword(s):  
Escherichia Coli ◽  
Virulence Genes ◽  
Foodborne Pathogen ◽  
Virulence Gene ◽  
Latex Agglutination ◽  
Health Concern ◽  
E Coli ◽  
Uremic Syndrome ◽  
Human Infections ◽  
Pcr Targeting

ABSTRACTBackgroundEscherichia coli O157 is an emerging foodborne pathogen of great public health concern. It has been associated with bloody diarrhoea, haemorrhagic colitis and haemolytic uremic syndrome in humans. Most human infections have been traced to cattle and the consumption of contaminated cattle products. In order to understand the risk associated with the consumption of cattle products, this study sought to investigate the prevalence and identify virulence genes in E. coli O157 from cattle in Cameroon.MethodA total of 512 rectal samples were obtained and analysed using conventional bacteriological methods (enrichment on modified Tryptone Soy Broth and selective plating on Cefixime-Tellurite Sorbitol Mac-Conkey Agar) for the isolation of E. coli O157. Presumptive E. coli O157 isolates were confirmed serologically using E. COLIPRO™ O157 latex agglutination test and molecularly using PCR targeting the rfb gene in the isolates. Characterisation of the confirmed E. coli O157 strains was done by amplification of stx1, stx2, eaeA and hlyA virulence genes using both singleplex and multiplex PCR.ResultsE. coli O157 was detected in 56 (10.9%) of the 512 samples examined. The presence of the virulence genes stx2, eaeA and hylA was demonstrated in 96.4% (54/56) of the isolates and stx1 in 40 (71.4%) of the 54. The isolates exhibited three genetic profiles (I-III) with I (stx1, stx2, eaeA and hlyA) being the most prevalent (40/56; 71.4%) while two isolates had none of the virulence genes tested.ConclusionA proportion of cattle slaughtered in abattoirs in Buea are infected with pathogenic E. coli O157 and could be a potential source of human infections. We recommend proper animal food processing measures and proper hygiene be prescribed and implemented to reduce the risk of beef contamination.

Comparison of Effects of Antimicrobial Interventions on Multidrug-Resistant Salmonella, Susceptible Salmonella, and Escherichia coli O157:H7†

2008 ◽  
Vol 71 (11) ◽  
pp. 2177-2181 ◽  
Author(s):  
TERRANCE M. ARTHUR ◽  
NORASAK KALCHAYANAND ◽  
JOSEPH M. BOSILEVAC ◽  
DAYNA M. BRICHTA-HARHAY ◽  
STEVEN D. SHACKELFORD ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Human Disease ◽  
Foodborne Pathogen ◽  
Multidrug Resistant ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Beef Processing ◽  
Processing Plants ◽  
Human Illness ◽  
Antimicrobial Interventions

Several strains of Salmonella have been identified as resistant to multiple antibiotics. What is not known is whether strains possessing multidrug resistance properties also have the ability to resist the killing effects of the antimicrobial interventions used in beef processing. The research project described herein was designed to determine whether antimicrobial interventions currently in place in beef processing facilities are adequate for reducing the foodborne pathogen loads on beef carcass surfaces contaminated with multidrug-resistant (MDR) Salmonella. The data presented here indicate that MDR Salmonella is reduced at least as effectively as are Escherichia coli O157:H7 and susceptible Salmonella when treated with antimicrobial interventions currently in use at most U.S. beef processing plants. The E. coli O157:H7 strains used in this study were divided into two groups, strains that have a genetic polymorphism associated with human disease and strains not typically found to cause human disease. No differences were detected in the abilities of these two strain types to survive antimicrobial interventions. These results indicate that neither the drug resistance status of a particular Salmonella strain nor the likelihood that a particular E. coli O157:H7 strain will cause human illness influences the antimicrobial efficacy of the interventions utilized by the modern beef processing plants.

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

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