scholarly journals First Isolation and Molecular Characterization of bla CTX-M-121 -producing Escherichia coli O157:H7 Strain Y4-A109 from Cattle in China

2020 ◽  
Author(s):  
Zhanqiang Su ◽  
Panpan Tong(Former Corresponding Author) ◽  
Ling Zhang ◽  
Mengmeng Zhang ◽  
Dong Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Horizontal Transmission ◽  
Virulence Gene ◽  
Multi Drug Resistance ◽  
Escherichia Coli O157 ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Cattle Farms

Abstract Background: To study the antibiotic resistance, the molecular epidemiology of bovine Escherichia coli ( E.coli ) O157:H7, and exploring the intrinsic relationship among different isolates, we have collected 27 bovine E. coli O157:H7 strains in Xinjiang from 2012 to 2017 and evaluated virulence genes, antibiotic resistance, and pulsed-field gel electrophoresis (PFGE) molecular typing. Results: Of all the 27 bovine E. coli O157:H7 strains analyzed, 21 strains contained at least one virulence gene, 19 strains carried eae gene (70.4%) and 8 of them carrying stx1 + stx2 + eae + hly + tccP . Most strains were sensitive to all the antibiotics tested. However, 4 of which were antibiotic-resistant, and 2 of which possessed multi-drug resistance, including one ESBL-producing strain. This is the first report of the bla CTX-M-121 gene in bovine E. coli O157:H7. Moreover, the bla CTX-M-121 gene can be transmitted horizontally through plasmid between strains. The similarity of PFGE spectra of 27 strains was between 65.8% and 100%. Two types of PFGE were obtained through cluster analysis, including clusters Ⅰ and Ⅱ. Conclusions: E. coli O157:H7 may have undergone clonal propagation in cattle farms as well as cross-regional transmission and horizontal transmission in different regions in Xinjiang China.

scholarly journals First Isolation and Molecular Characterization of blaCTX-M-121-producting Escherichia coli O157:H7 from China Cattle

2019 ◽  
Author(s):  
Zhanqiang Su ◽  
Panpan Tong ◽  
Ling Zhang ◽  
Mengmeng Zhang ◽  
Dong Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Clonal Propagation ◽  
Horizontal Transmission ◽  
Virulence Gene ◽  
Multi Drug Resistance ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Cattle Farms

Abstract Background: To study antibiotic resistance and the molecular epidemiology of bovine Escherichia coli (E.coli) O157:H7 and to explore the intrinsic relationship among different isolates, we collected 27 strains of bovine E. coli O157:H7 in Xinjiang from 2012 to 2017 and assessed virulence genes, antibiotic resistance and pulsed-field gel electrophoresis (PFGE) molecular typing. Results: In this study, 21 strains carried at least one virulence gene, and 19 strains carried eae gene (70.4%) including 8 carrying stx1+stx2+eae +hly+tccP. Most strains were sensitive to all antibiotics tested, 4 strains were bacteria-resistant, and 2 strains possessed multi-drug resistance, including one ESBL-producing strain. This is the first report of a blaCTX-M-121 gene in bovine E. coli O157:H7. Moreover, blaCTX-M-121 gene can be transmitted horizontally by plasmid between strains. The PFGE spectral similarity of the 27 strains was between 65.8% and 100%. Two PFGE types including clusters Ⅰ and Ⅱ were obtained through cluster analysis. Conclusions: E. coli O157:H7 may have undergone clonal propagation in cattle farms as well as cross-regional transmission. A horizontal transmission path with E. coli O157:H7 appeared in different areas.

scholarly journals First Isolation and Molecular Characterization of blaCTX-M-121-Producing Escherichia coli O157:H7 From Cattle in Xinjiang, China

2021 ◽  
Vol 8 ◽  
Author(s):  
Zhanqiang Su ◽  
Panpan Tong ◽  
Ling Zhang ◽  
Mengmeng Zhang ◽  
Dong Wang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogen ◽  
Virulence Gene ◽  
Multidrug Resistant ◽  
Escherichia Coli O157 ◽  
Bacterial Strains ◽  
E Coli ◽  
Uremic Syndrome ◽  
Cattle Farms

The bovine Escherichia coli O157:H7 is a major foodborne pathogen causing severe bloody diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome in humans. Cattle are recognized major reservoir and source of E. coli O157:H7. We investigated the antibiotic resistance, molecular profiles, and intrinsic relationship between 21 isolates of E. coli O157:H7 from cattle farms and slaughtering houses in Xinjiang. Using pulsed-field gel electrophoresis (PFGE) molecular typing, two types of PFGE were revealed through cluster analysis, including clusters I and II, with 66 and 100% similarity of PFGE spectra between 21 isolates. We also detected that 18 isolates (86%) carried at least one virulence gene, 16 isolates (76%) carried the eae gene, and 7 (33%) carried the stx1 + stx2 + eae + hly + tccp genes. Eighteen isolates were susceptible to antibiotics. Three isolates were resistant to antibiotics, and two were multidrug resistant. One of the two multidrug-resistant isolates detectably carried the blaCTX−M−121 gene. This is the first finding of the blaCTX−M−121 gene detected in E. coli O157:H7 isolated from cattle in Xinjiang. The blaCTX−M−121 gene is transferable between the bacterial strains via plasmid transmission. The results indicated that E. coli O157:H7 may have undergone clonal propagation in cattle population and cross-regional transmission in Xinjiang, China.

Characterization of Antibiotic Resistance E. Coli and Antibiotic Resistance Genes in Aquatic Environment of Taihu Lake, China

2013 ◽  
Vol 295-298 ◽  
pp. 630-634 ◽  
Author(s):  
Ni Ni Han ◽  
Song He Zhang ◽  
Pei Fang Wang ◽  
Chao Wang
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Surface Water ◽  
Resistance Genes ◽  
Taihu Lake ◽  
Antibiotic Resistance Genes ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Realtime Pcr

The aims of this study are to evaluate multiple antibiotic resistant Escherichia coli isolated from surface water and to investigate the presence and distribution antibiotic resistance genes (ARGs) in sediments of Taihu Lake. The results show that the presentence of four ARGs concentrations in the sediments of the lake was in sequence: strB>qnrB>strA>qnrS, as determined by realtime-PCR technique. The southwest and east areas of Taihu Lake were polluted seriously than other areas from all kinds of antibiotics. The screening Escherichia coli had a higher resistance to streptomycin, tetracycline and ampicillin than other four antibiotics, and had a lowest resistance to levofloxacin.

scholarly journals Detection of Antibiotic Resistance Genes in Some Multiple Antibiotic Resistant E. coli from Apparently Healthy Pregnant Women

2020 ◽  
pp. 23-31
Author(s):  
O. C. Adekunle ◽  
A. J. Falade- Fatila ◽  
R. Ojedele ◽  
G. Odewale
Keyword(s):  
Escherichia Coli ◽  
Drug Resistance ◽  
Antibiotic Resistance ◽  
Pregnant Women ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Multi Drug Resistance ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Apparently Healthy

The emerging drug resistance, especially among the Escherichia coli (E.coli) isolates from pregnant women, spread rapidly within the community. Urinary tract infection (UTI) is a well-known bacterial infection posing serious health problem in pregnant women. Also, multi-drug resistance is becoming rampant, and it is of serious public health concern. Treatment of E. coli is now a challenge due to continuous increase in resistance towards commonly prescribed antibiotics, thus posing a threat to treatment. Hence, the aim of the study is to determine antibiotic resistance genes in some multiple antibiotic resistant E.coli from apparently healthy pregnant women in Osun State. A cross-sectional study design was used to collect 150 mid-stream urine samples from apparently healthy pregnant women from March, 2018 to September, 2018. A well structured questionnaire and informed consent were used for data collection. Standard loop technique was used to place 0.001 ml of urine on Cysteine Lactose Electrolyte Deficient (CLED) medium, Blood agar, MacConkey agar and incubated at 37 °C for 24 h. A standard agar disc diffusion method was used to determine antimicrobial susceptibility pattern of the isolates. The molecular detection of the resistant genes was done using PCR techniques. The ages of women enrolled in this study ranges from 22 to 42 years (mean ± standard deviation = 31 ± 4.7 years). Escherichia coli showed high percentage of resistance to ampicillin and low resistance to ciprofloxacin and penicillin. All the E. coli isolates were sensitive to levofloxacin, and most were resistant to Meropenem. Multiple drug resistance was observed in all the isolates. Resistance genes in VIM 390bp, bla ctx-M 585bp and TEM 517bp were detected in some of the representative E. coli isolates profiled. This study identified the presence of Multi-drug resistance genes in E. coli associated UTI among pregnant women in Osogbo.

scholarly journals Prevalence and antibiotic resistance profile of Shiga-toxigenic Escherichia coli O157 (STEC) from retailed miscellaneous meat and fish types in Abuja, Nigeria

2021 ◽  
Vol 2 (2) ◽  
pp. 37-43
Author(s):  
Adaeze Joy Alu ◽  
Gabriel K. Omeiza ◽  
James A. Ameh ◽  
Enem S.I
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Enrichment Culture ◽  
Intestinal Bacteria ◽  
Latex Agglutination ◽  
Multi Drug Resistance ◽  
Escherichia Coli O157 ◽  
Resistance Profile ◽  
E Coli ◽  
Antibiotic Resistance Profile

Most Escherichia coli strains are harmless intestinal bacteria of animals, but some are implicated in food infection/poisoning especially Shiga toxin (or Vero toxin) producing E. coli (STEC) due to consumption of meat. This study was conducted to determine the prevalence and antibiotic resistance profile of Shigatoxigenic Escherichia coli O157 (STEC) from retailed miscellaneous fish and meat types in Abuja, Federal Capital Territory, Nigeria. A total of 256 meat and fish consisting of cow muscles, intestines, rumen-sacs, livers and tails, cat-fish, frozen fish (mackerel and herrings) were examined. Escherichia coli were isolated by enrichment culture cefixime-tellurite sorbitol MacConkey agar (CT-SMAC), morphological, biochemical, serotype latex agglutination and disk diffusion methods. Of the 256 samples, 138 (53.9%) were contaminated with E. coli and 28 (21.7%) E. coli strains were positive for Shigatoxigenic Escherichia coli O157 (STEC). Meat muscles had the highest prevalence of STEC (7.41%) among meat samples, followed by rumen-sacs (6.0%), intestines (5.77%), tails (4.0%), and the prevalence of STEC in Fish includes Cat-fish intestine (26.7%), skin (21.4%), Mackerel intestine (26.7%), skin (14.3%), and Herrings skin (15.4%), gill (7.1%). All the STEC assessed indicated multi-drug resistance, with the isolates showing 100% resistant to ampicilin, and erythromycin, nitrofurantoin (95.7%), amoxicilin clavulanic acid (84.3%), sulphamethaxazole/trimethoprim (75%), streptomycin (75%), tetracycline (66.17%), and gentamycin (53.6%). The isolates were susceptible to ciprofloxacin (66.7%), Cefoxitin (66.7%), amikacin (39.3%), and chloramphenicol (35.7%). The implication of STEC in this study suggests that contaminated meat types are sold to consumers and can result to serious foodborne hazards. Prescription of ciprofloxacin and cefoxicilin are recommended against this organism. Application of good hygienic procedures in meat and fish handling processes and proper boiling before consumption can mitigate the risk of infection due to resistance STEC strains.

scholarly journals Characterization of Tn7-like transposons and its related antibiotic resistance among Enterobacteriaceae from livestock and poultry in China

2020 ◽  
Author(s):  
Juan He ◽  
Cui Li ◽  
Pengfei Cui ◽  
Hongning Wang
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Horizontal Transmission ◽  
Antibiotic Resistance Genes ◽  
Variable Region ◽  
Multidrug Resistant ◽  
Antibiotic Resistant ◽  
E Coli ◽  
New Type

Abstract Background: This study was aimed to investigate the prevalence and structure of Tn7-like in Enterobacteriaceae from livestock and poultry as well as their possible role as reservoir of antibiotic resistance genes (ARGs).Methods: Polymerase chain reaction (PCR) and DNA sequencing analyses were used for the characterization of Tn7-like, associated integrons and ARGs. The antimicrobial resistance profile of the isolates was examined by using disc diffusion test.Results: Three hundred and seventy-eight Tn7-like-positive strains of Enterobacteriaceae were isolated, and included E. coli (128), Proteus(150), K. pneumonia(17), Salmonella(13), M. morganii (21) and A. baumannii(1), wherein high resistance was observed for Trimethoprim/Sulfamethoxazole and Streptomycin, and fifty percent of the strains were multidrug-resistant. Integrons class 2 were detected in all of the isolates and there are high frequency mutation sites especially in 535, a stop mutation. Variable region of class 2 integrons carried same gene cassettes, namely aadA1-sat2-dfrA1. From the 378 isolated strains, we found a new type of Tn7-like on a plasmid, named Tn6765.Conclusions: These findings proved that the Tn7-like can contribute to the horizontal transmission of antibiotic resistant genes in livestock and poultry. As potential vessels for antibiotic resistance genes (ARGs), Tn7-like could not be ignored due to their efficient transfer ability in environments.

Characterization of Colicinogenic Escherichia coli Strains Inhibitory to Enterohemorrhagic Escherichia coli

2004 ◽  
Vol 67 (3) ◽  
pp. 486-492 ◽  
Author(s):  
GERRY P. SCHAMBERGER ◽  
FRANCISCO DIEZ-GONZALEZ
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Food Safety ◽  
Virulence Factors ◽  
Enterohemorrhagic Escherichia Coli ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Strain Collection ◽  
Safety Intervention

A previously identified set of anti– Escherichia coli O157:H7 colicinogenic E. coli were characterized to assess the suitability of these isolates as a preharvest food safety intervention in cattle. This collection of 23 E. coli strains were screened for virulence factors, antibiotic resistance, type of colicin(s) present, and their ability to inhibit other pathogenic E. coli. With the use of PCR, pathogen genes were detected in six of the 23 colicinogenic E. coli. When the nonpathogenic strains were assessed for antibiotic resistance, four strains showed resistance to at least one antibiotic. The remaining set of 14 strains were evaluated for the presence of previously identified colicins. Seven colicins (B, E1, E2/E7, E7, Ia/Ib, K, and M) were detected. One half of the strains possessed multiple types of colicins. The most commonly detected colicins were B, E2/E7, and M, which were found in six strains each. DNA sequencing was also performed in order to classify the E2/E7 colicins separately from E7 colicins. The 14 colicinogenic E. coli also were evaluated for their ability to inhibit 10 different non-O157 pathogenic E. coli. Six of the colicinogenic E. coli were capable of inhibiting all 10 pathogens, and the remaining eight strains could each inhibit between six to eight of the pathogenic E. coli. This strain collection has great potential for inhibiting E. coli O157:H7 in cattle.

scholarly journals Mechanisms of Resistance in Multiple-Antibiotic-Resistant Escherichia coli Strains of Human, Animal, and Food Origins

2004 ◽  
Vol 48 (10) ◽  
pp. 3996-4001 ◽  
Author(s):  
Yolanda Sáenz ◽  
Laura Briñas ◽  
Elena Domínguez ◽  
Joaquim Ruiz ◽  
Myriam Zarazaga ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Amino Acid ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Mechanisms Of Resistance ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Class 1 ◽  
Human Animal

ABSTRACT Seventeen multiple-antibiotic-resistant nonpathogenic Escherichia coli strains of human, animal, and food origins showed a wide variety of antibiotic resistance genes, many of them carried by class 1 and class 2 integrons. Amino acid changes in MarR and mutations in marO were identified for 15 and 14 E. coli strains, respectively.

scholarly journals Molecular Characterization of Plasmid-Mediated Non-O157 Verotoxigenic Escherichia coli Isolated from Infants and Children with Diarrhea

2020 ◽  
Vol 17 (3) ◽  
pp. 0710
Author(s):  
Md Fazlul Karim Khan ◽  
Shah Samiur Rashid
Keyword(s):  
Escherichia Coli ◽  
Virulence Gene ◽  
Multidrug Resistant ◽  
Plasmid Curing ◽  
Health Issues ◽  
E Coli ◽  
Disk Diffusion Assay ◽  
Microbiological Techniques ◽  
Polymerase Chain

A significant increase in the incidence of non-O157 verotoxigenic Escherichia coli (VTEC) infections have become a serious health issues, and this situation is worsening due to the dissemination of plasmid mediated multidrug-resistant microorganisms worldwide. This study aims to investigate the presence of plasmid-mediated verotoxin gene in non-O157 E. coli. Standard microbiological techniques identified a total of 137 E. coli isolates. The plasmid was detected by Perfectprep Plasmid Mini preparation kit. These isolates were subjected to disk diffusion assay, and plasmid curing with ethidium bromide treatment. The plasmid containing isolates were subjected to a polymerase chain reaction (PCR) for investigating the presence of plasmid mediated verotoxin gene (VT1 and VT2) in non-O157 E. coli. Among the 137 E. coli isolates, 49 isolates were non-O157 E. coli while 29 (59.1%) isolates were verotoxin producing non-O157 serotypes and 26 non-O157 VTEC isolates possessed plasmids. Certain isolates harboured single sized plasmid while others had multiple plasmids with different size varied from 1.8kb to 7.6kb. A plasmid containing all (100%) the isolates was multidrug-resistant. Eight isolates changed their susceptibility patterns while three isolates were found to lose plasmid after post plasmid curing treatment and the rest of the isolates (15) remained constant. Different PCR sets characterized 3 plasmid-mediated verotoxins producing non-O157 E. coli. This current study demonstrated the occurrence of plasmid mediated verotoxin gene in non-O157 E. coli. To the best of our knowledge, this is the first report in the global literature on plasmid-mediated verotoxin gene in non-O157 E. coli. Timely diagnosis and surveillance of VTEC infections should prioritize to stop or slow down the virulence gene for dissemination by plasmid-mediated gene transfer amongst the same bacteria or other species.

scholarly journals Antibiotic-resistant Escherichia coli and Salmonella spp. associated with dairy cattle and farm environment having public health significance

2019 ◽  
Vol 12 (7) ◽  
pp. 984-993 ◽  
Author(s):  
Md. Abdus Sobur ◽  
Abdullah Al Momen Sabuj ◽  
Ripon Sarker ◽  
A. M. M. Taufiqur Rahman ◽  
S. M. Lutful Kabir ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Resistance Genes ◽  
One Health ◽  
Dairy Farm ◽  
Antibiotic Resistance Genes ◽  
Salmonella Spp ◽  
Antibiotic Resistant ◽  
E Coli

Aim: The present study was carried out to determine load of total bacteria, Escherichia coli and Salmonella spp. in dairy farm and its environmental components. In addition, the antibiogram profile of the isolated bacteria having public health impact was also determined along with identification of virulence and resistance genes by polymerase chain reaction (PCR) under a one-health approach. Materials and Methods: A total of 240 samples of six types (cow dung - 15, milk - 10, milkers' hand wash - 10, soil - 10 water - 5, and vegetables - 10) were collected from four dairy farms. For enumeration, the samples were cultured onto plate count agar, eosin methylene blue, and xylose-lysine deoxycholate agar and the isolation and identification of the E. coli and Salmonella spp. were performed based on morphology, cultural, staining, and biochemical properties followed by PCR. The pathogenic strains of E. coli stx1, stx2, and rfbO157 were also identified through PCR. The isolates were subjected to antimicrobial susceptibility test against 12 commonly used antibiotics by disk diffusion method. Detection of antibiotic resistance genes ereA, tetA, tetB, and SHV were performed by PCR. Results: The mean total bacterial count, E. coli and Salmonella spp. count in the samples ranged from 4.54±0.05 to 8.65±0.06, 3.62±0.07 to 7.04±0.48, and 2.52±0.08 to 5.87±0.05 log colony-forming unit/g or ml, respectively. Out of 240 samples, 180 (75%) isolates of E. coli and 136 (56.67%) isolates of Salmonella spp. were recovered through cultural and molecular tests. Among the 180 E. coli isolates, 47 (26.11%) were found positive for the presence of all the three virulent genes, of which stx1 was the most prevalent (13.33%). Only three isolates were identified as enterohemorrhagic E. coli. Antibiotic sensitivity test revealed that both E. coli and Salmonella spp. were found highly resistant to azithromycin, tetracycline, erythromycin, oxytetracycline, and ertapenem and susceptible to gentamycin, ciprofloxacin, and imipenem. Among the four antibiotic resistance genes, the most observable was tetA (80.51-84.74%) in E. coli and Salmonella spp. and SHV genes were the lowest one (22.06-25%). Conclusion: Dairy farm and their environmental components carry antibiotic-resistant pathogenic E. coli and Salmonella spp. that are potential threat for human health which requires a one-health approach to combat the threat.

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