scholarly journals Citric Acid Promoting B Lymphocyte Differentiation and Anti-epithelial Cells Apoptosis Mediate the Protective Effects of Hermetia illucens Feed in ETEC Induced Piglets Diarrhea

2021 ◽  
Vol 8 ◽  
Author(s):  
Mingming Liu ◽  
Boyu Yuan ◽  
Xinxin Jin ◽  
Mingqiang Zhu ◽  
Haidong Xu ◽  
...  
Keyword(s):  
Citric Acid ◽  
Immune Modulation ◽  
B Lymphocyte ◽  
Intestinal Barrier ◽  
Poultry Production ◽  
Protective Effects ◽  
Weaned Piglets ◽  
Hermetia Illucens ◽  
Lymphocyte Differentiation ◽  
Etec Infection

Newborn piglets are prone to diarrhea after weaning as a result of changes in their environment and feed. Enterotoxigenic Escherichia coli (ETEC) K88 strain is a typical pathogen that causes diarrhea in such stage of piglets. Hermetia illucens larvae are widely used in livestock and poultry production because of their high nutritional value and immunoregulatory effects. This study aimed to evaluate the protective effects of H. illucens feed in protecting against ETEC induced diarrhea in piglets and to unravel the mechanisms of immune modulation and intestinal barrier maintenance. The results showed that after ETEC infection, citric acid in the serum of the groups fed on H. illucens larvae increased significantly, which stimulated macrophages to secrete cytokines that promote B lymphocyte differentiation, ultimately increasing the production of IgA and IgG in serum. Concomitantly, citric acid also had a positive effect on the intestinal barrier damaged due to ETEC infection by inhibiting the production of inflammatory cytokines, reducing the Bcl-2/Bax ratio, and promoting the expression of tight junction proteins. Correlation analysis showed that the increase of citric acid levels might be related to Massilia. Thus, citric acid derived from H. illucens larvae can improve the immune performance of weaned piglets and reduce ETEC-induced damage to the intestinal barrier in weaned piglets.

scholarly journals Effect of Hermetia Illucens Larvae on Growth Performance of Weaned Piglets and Diarrhea Caused by Escherichia Coli K88

2021 ◽  
Author(s):  
Xinxin Jin ◽  
Boyu Yuan ◽  
Mingming Liu ◽  
Mingqiang Zhu ◽  
Xue Zhang ◽  
...  
Keyword(s):  
Tight Junction ◽  
Growth Performance ◽  
Intestinal Mucosa ◽  
Fish Meal ◽  
Intestinal Barrier ◽  
Inflammatory Factor ◽  
Weaned Piglets ◽  
Large White ◽  
Hermetia Illucens ◽  
Junction Protein

Abstract Background：A high-quality protein substitute, Hermetia illucens (Black soldier fly) larvae powder is rich in protein, fat, amino acid, calcium, and other substances. Due to the relatively few studies on the feeding of weaned piglets, in the present study, we replaced part or all of the fish meal with a relevant proportion of Hermetia illucens larvae powder in the feed to study its effect on weaned piglets. A total of forty-eight young female weaned piglets (Duroc ´ Landrace ´ Large White) with initial body weights (BW) 7.68 ± 0.26 kg, were randomly divided into three groups, each group had eight replicates, two pigs per replicate. Three groups containing different proportions of Hermetia illucens larvae powder (0, 4%, and 8%) were referred to as C, HI4, and HI8. We designed a 28-day feeding experiment, collecting blood and feces, thereafter inducing the piglets with oral gavage of ETEC K88 and recording diarrhea on day 29 of the experiment. Four piglets in each group were selected to collect serum, colon contents, intestinal tissue, and jejunum, ileum, colon mucosa samples.Results：The growth performance of weaned piglets remained unaffected on supplementing feed with Hermetia illucens. Compared with C+K88 group, the diarrhea rate was found to be attenuated for the Hermetia illucens supplemented group. Severe damage was evident in the case of the ileum villi of the C+K88 group, whereas improved integrity was demonstrated by the ileum villi of the HI4+K88 and HI8+K88 groups. Significantly increased expressions of the anti-inflammatory factor IL-10 in the intestinal mucosa (P < 0.05) and the tight junction proteins Occludin and Claudin-3 (P < 0.05) and reduced expression of the pro-inflammatory factor TNF-α (P < 0.05) were reflected in the HI4+K88 and HI8+K88 groups as compared to the C+K88 group. The activity of antioxidant enzymes CAT and POD (P < 0.05) also revealed an effective increase in the Hermetia illucens supplemented groups than the control. The results of immunoblotting also validated that the same ETEC K88 treatment of weaned piglets enhanced the expression of tight junction protein in the intestinal mucosa of the Hermetia illucens addition group (P < 0.05) and the integrity of the intestinal barrier was also better maintained. The experimental results affirmed that Hermetia illucens larvae powder could partially or completely replace fish meal, increase the content of Lactobacillus, reduce the content of Streptococcus, improve the body’s disease resistance, and improve intestinal health.Conclusions：ETEC-induced diarrhea will be reduced by the diet of weaned piglets containing Hermetia illucens larvae, ameliorating the immune performance of pigs. The present research provides a new perspective for insect meal as a sustainable protein source for pig feed.

Influence of Plant Bioactive Compounds on Intestinal Epithelial Barrier in Poultry

2020 ◽  
Vol 20 (7) ◽  
pp. 566-577 ◽  
Author(s):  
Amlan Kumar Patra
Keyword(s):  
Bioactive Compounds ◽  
Barrier Function ◽  
Molecular Mechanisms ◽  
Digestive Enzyme ◽  
Intestinal Barrier ◽  
Feed Additives ◽  
Interactive Effects ◽  
Intestinal Barrier Function ◽  
Poultry Production ◽  
Poultry Feeding

Natural plant bioactive compounds (PBC) have recently been explored as feed additives to improve productivity, health and welfare of poultry following ban or restriction of in-feed antibiotic use. Depending upon the types of PBC, they possess antimicrobial, digestive enzyme secretion stimulation, antioxidant and many pharmacological properties, which are responsible for beneficial effects in poultry production. Moreover, they may also improve the intestinal barrier function and nutrient transport. In this review, the effects of different PBC on the barrier function, permeability of intestinal epithelia and their mechanism of actions are discussed, focusing on poultry feeding. Dietary PBC may regulate intestinal barrier function through several molecular mechanisms by interacting with different metabolic cascades and cellular transcription signals, which may then modulate expressions of genes and their proteins in the tight junction (e.g., claudins, occludin and junctional adhesion molecules), adherens junction (e.g., E-cadherin), other intercellular junctional proteins (e.g., zonula occludens and catenins), and regulatory proteins (e.g., kinases). Interactive effects of PBC on immunomodulation via expressions of several cytokines, chemokines, complement components, pattern recognition receptors and their transcription factors and cellular immune system, and alteration of mucin gene expressions and goblet cell abundances in the intestine may change barrier functions. The effects of PBC are not consistent among the studies depending upon the type and dose of PBC, physiological conditions and parts of the intestine in chickens. An effective concentration in diets and specific molecular mechanisms of PBC need to be elucidated to understand intestinal barrier functionality in a better way in poultry feeding.

scholarly journals Clostridium Butyricum ZJU-F1 Benefits the Intestinal Barrier Function and Immune Response Associated with Its Modulation of Gut Microbiota in Weaned Piglets

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 527
Author(s):  
Jie Fu ◽  
Tenghao Wang ◽  
Xiao Xiao ◽  
Yuanzhi Cheng ◽  
Fengqin Wang ◽  
...  
Keyword(s):  
Immune Response ◽  
Bacillus Licheniformis ◽  
Barrier Function ◽  
Intestinal Barrier ◽  
Clostridium Butyricum ◽  
Intestinal Barrier Function ◽  
Weaned Piglets ◽  
Total Acid ◽  
Junction Protein ◽  
Mucin Expression

This study investigated the effects of dietary C. butyricum ZJU-F1 on the apparent digestibility of nutrients, intestinal barrier function, immune response, and microflora of weaned piglets, with the aim of providing a theoretical basis for the application of Clostridium butyricum as an alternative to antibiotics in weaned piglets. A total of 120 weanling piglets were randomly divided into four treatment groups, in which piglets were fed a basal diet supplemented with antibiotics (CON), Bacillus licheniformis (BL), Clostridium butyricum ZJU-F1 (CB), or Clostridium butyricum and Bacillus licheniformis (CB-BL), respectively. The results showed that CB and CB-BL treatment increased the intestinal digestibility of nutrients, decreased intestinal permeability, and increased intestinal tight junction protein and mucin expression, thus maintaining the integrity of the intestinal epithelial barrier. CB and CB-BL, as exogenous probiotics, were also found to stimulate the immune response of weaned piglets and improve the expression of antimicrobial peptides in the ileum. In addition, dietary CB and CB-BL increased the proportion of Lactobacillus. The levels of butyric acid, propionic acid, acetic acid, and total acid were significantly increased in the ceca of piglets fed CB and CB-BL. Furthermore, we validated the effects of C. butyricum ZJU-F1 on the intestinal barrier function and immune response in vitro and found C. butyricum ZJU-F1 improved intestinal function and enhanced the TLR-2-MyD88-NF-κB signaling.

B-lymphocyte differentiation in lethally irradiated and reconstituted mice

1977 ◽  
Vol 31 (2) ◽  
pp. 340-348 ◽  
Author(s):  
J. Rozing ◽  
N.H.C. Brons ◽  
R. Benner
Keyword(s):  
B Lymphocyte ◽  
Lymphocyte Differentiation

scholarly journals Supplementation of a lacto-fermented rapeseed-seaweed blend promotes gut microbial- and gut immune-modulation in weaner piglets

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yan Hui ◽  
Paulina Tamez-Hidalgo ◽  
Tomasz Cieplak ◽  
Gizaw Dabessa Satessa ◽  
Witold Kot ◽  
...  
Keyword(s):  
Immune Modulation ◽  
Amplicon Sequencing ◽  
Saccharina Latissima ◽  
Rrna Gene ◽  
Microbiota Composition ◽  
Colon Mucosa ◽  
Weaned Piglets ◽  
Brown Macroalgae ◽  
Blood Concentrations ◽  
Significant Difference

Abstract Background The direct use of medical zinc oxide in feed will be abandoned after 2022 in Europe, leaving an urgent need for substitutes to prevent post-weaning disorders. Results This study investigated the effect of using rapeseed-seaweed blend (rapeseed meal added two brown macroalgae species Ascophylum nodosum and Saccharina latissima) fermented by lactobacilli (FRS) as feed ingredients in piglet weaning. From d 28 of life to d 85, the piglets were fed one of three different feeding regimens (n = 230 each) with inclusion of 0%, 2.5% and 5% FRS. In this period, no significant difference of piglet performance was found among the three groups. From a subset of piglets (n = 10 from each treatment), blood samples for hematology, biochemistry and immunoglobulin analysis, colon digesta for microbiome analysis, and jejunum and colon tissues for histopathological analyses were collected. The piglets fed with 2.5% FRS manifested alleviated intraepithelial and stromal lymphocytes infiltration in the gut, enhanced colon mucosa barrier relative to the 0% FRS group. The colon microbiota composition was determined using V3 and V1-V8 region 16S rRNA gene amplicon sequencing by Illumina NextSeq and Oxford Nanopore MinION, respectively. The two amplicon sequencing strategies showed high consistency between the detected bacteria. Both sequencing strategies indicated that inclusion of FRS reshaped the colon microbiome of weaned piglets with increased Shannon diversity. Prevotella stercorea was verified by both methods to be more abundant in the piglets supplied with FRS feed, and its abundance was positively correlated with colonic mucosa thickness but negatively correlated with blood concentrations of leucocytes and IgG. Conclusions FRS supplementation relieved the gut lymphocyte infiltration of the weaned piglets, improved the colon mucosa barrier with altered microbiota composition. Increasing the dietary inclusion of FRS from 2.5% to 5% did not lead to further improvements.

scholarly journals Calcium anacardate and citric acid as growth promoters for weaned piglets

2020 ◽  
Vol 238 ◽  
pp. 104084
Author(s):  
J.L. Ferreira ◽  
P.H. Watanabe ◽  
I.B. Mendonça ◽  
B.D. Nogueira ◽  
A.C.S. Ferreira ◽  
...  
Keyword(s):  
Citric Acid ◽  
Weaned Piglets ◽  
Growth Promoters
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