scholarly journals Characterization of Streptomyces spp. from Rice Fields as a Potential Biocontrol Agent against Burkholderia glumae and Rice Plant Growth Promoter

Agronomy ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1850
Author(s):  
Mohamad Syazwan Ngalimat ◽  
Erneeza Mohd Hata ◽  
Dzarifah Zulperi ◽  
Siti Izera Ismail ◽  
Mohd Razi Ismail ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Biocontrol Agent ◽  
Growth Promotion ◽  
Antagonistic Activity ◽  
Rice Fields ◽  
Burkholderia Glumae ◽  
Pgp Traits ◽  
Genus Streptomyces ◽  
Streptomyces Sp

The usage of plant growth-promoting (PGP) Streptomyces to improve plant growth is an alternative to existing strategies of chemical fertilizers that commonly caused environmental pollution. The aim of this study was to isolate and characterize PGP Streptomyces from Malaysian rice fields with antagonistic activity against Bacterial Panicle Blight disease in rice caused by Burkholderia glumae. A total of 50 bacterial isolates were recovered from the soil, rhizosphere, and endosphere; 22% showed antagonistic activity against B. glumae. Molecular identification using 16S rRNA amplification and phylogenetic tree analyses revealed that the antagonistic isolates belonged to the genus Streptomyces. Among the antagonistic isolates, Streptomyces sp. TBS5 (26.35 ± 0.14 mm) demonstrated the most antagonistic activity (p < 0.05) against B. glumae. Through API® ZYM analysis, the antagonistic isolates were revealed to have phosphoric hydrolase, ester hydrolase, peptide hydrolase, and glycosidase activities that play a crucial role in plant growth promotion. The antagonistic isolates demonstrated the highest (80%) PGP traits including able to fix nitrogen and solubilize phosphate, as well as produce siderophore and indole-3-acetic acid. Plant growth promotion assay under laboratory and greenhouse conditions were analyzed with the treatment of rice, Oryza sativa, seeds with the antagonistic isolates. Seeds treated with Streptomyces sp. TBS5, JAS2, R2-7, and TKR8 showed improvement in vigor index by more than 100% compared to water-treated control plants under both conditions. Augmentation of root length showed an overall increment of more than 101.5% under laboratory condition and 151.9% under greenhouse condition using Streptomyces sp. TBS5, JAS2, R2-7, and TKR8 treatments. Results suggest that Streptomyces sp. TBS5, JAS2, R2-7, and TKR8 are a good candidate to be developed as both biocontrol agent against B. glumae and PGP agent in rice.

scholarly journals Exploitation of endophytic Pseudomonas sp. for plant growth promotion and colonization in rice

2017 ◽  
Vol 9 (3) ◽  
pp. 1310-1316
Author(s):  
Gurjot Kaur ◽  
Poonam Sharma ◽  
Deepika Chhabra ◽  
Kailash Chand ◽  
Gurjit Singh Mangat
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Pseudomonas Sp ◽  
Bacterial Isolates ◽  
Pgp Traits ◽  
P Solubilization ◽  
Fluorescent Pigment

The present investigation was carried out to exploit bacterial endophytes associated with root and leaf tissue of rice plant for plant growth promotion (PGP) and colonization study in vitro. Total 10 endophytic bacterial isolates (Pseudomonas sp.) were evaluate for PGP traits like P solubilization, production of Indole acetic acid (IAA), siderophore, ACC deaminase, protease, cellulase, fluorescent pigment, urease and denitrification activity. Out of 10 endophytic bacteria 30 %, 60 %, 20 %, 70 %, 10 % and 10 % were positive for siderophore, protease, cellulase, fluorescent pigment, urease and denitrification respectively. Maximum IAA production was recorded with isolate LRBLE7 (18.8 μgml-1) followed by LRBRE4 (16.0 μgml-1) and maximum P-solubilization was recorded with isolate LRBRE4 (5.8 mg 100 ml-1) followed by LRBLE7 (4.4 mg 100 ml-1). ACC deaminase production was recorded with isolate LRBLE6 (O.D=0.352 nm) followed by LRBRE5 (O.D=0.324nm). Three potential isolates (LRBRE4, LRBRE6 and LRBLE7) were selected on the basis of multiple PGP traits and were subjected to colonization study of rice seedling in vitro. Potential bacterial isolates can be exploited for improving growth and productivity in rice under sustainable management system.

scholarly journals Draft Genome Sequence of the Antifungal-Producing Plant-Benefiting Bacterium Burkholderia pyrrocinia CH-67

2012 ◽  
Vol 194 (23) ◽  
pp. 6649-6650 ◽  
Author(s):  
Ju Yeon Song ◽  
Min-Jung Kwak ◽  
Kwang Youll Lee ◽  
Hyun Gi Kong ◽  
Byung Kwon Kim ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Plant Growth ◽  
Forest Soil ◽  
Genome Sequence ◽  
Plant Growth Promotion ◽  
Biocontrol Agent ◽  
Growth Promotion ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Content Type

ABSTRACTBurkholderia pyrrociniaCH-67 was isolated from forest soil as a biocontrol agent to be utilized in agriculture. Here, we report the 8.05-Mb draft genome sequence of this bacterium. Its genome contains genes involved in biosynthesis of secondary metabolites and plant growth promotion, which may contribute to probiotic effects on plants.

scholarly journals Diversity, Phylogeny and Plant Growth Promotion Traits of Nodule Associated Bacteria Isolated from Lotus parviflorus

2020 ◽  
Vol 8 (4) ◽  
pp. 499 ◽  
Author(s):  
Ricardo Soares ◽  
Jesús Trejo ◽  
Maria J. Lorite ◽  
Etelvina Figueira ◽  
Juan Sanjuán ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Root Nodules ◽  
Nifh Gene ◽  
Bacterial Strains ◽  
Lytic Enzymes ◽  
Taxonomic Assignment ◽  
Pgp Traits ◽  
Associated Bacteria

Lotus spp. are widely used as a forage to improve pastures, and inoculation with elite rhizobial strains is a common practice in many countries. However, only a few Lotus species have been studied in the context of plant-rhizobia interactions. In this study, forty highly diverse bacterial strains were isolated from root nodules of wild Lotus parviflorus plants growing in two field locations in Portugal. However, only 10% of these isolates could nodulate one or more legume hosts tested, whereas 90% were thought to be opportunistic nodule associated bacteria. Phylogenetic studies place the nodulating isolates within the Bradyrhizobium genus, which is closely related to B. canariense and other Bradyrhizobium sp. strains isolated from genistoid legumes and Ornithopus spp. Symbiotic nodC and nifH gene phylogenies were fully consistent with the taxonomic assignment and host range. The non-nodulating bacteria isolated were alpha- (Rhizobium/Agrobacterium), beta- (Massilia) and gamma-proteobacteria (Pseudomonas, Lysobacter, Luteibacter, Stenotrophomonas and Rahnella), as well as some bacteroidetes from genera Sphingobacterium and Mucilaginibacter. Some of these nodule-associated bacteria expressed plant growth promotion (PGP) traits, such as production of lytic enzymes, antagonistic activity against phytopathogens, phosphate solubilization, or siderophore production. This argues for a potential beneficial role of these L. parviflorus nodule-associated bacteria.

scholarly journals Endophytic Burkholderia sp. SSG as a potential biofertilizer promoting boxwood growth

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9547
Author(s):  
Ping Kong ◽  
Chuanxue Hong
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Plant Protection ◽  
Growth Promotion ◽  
Agar Plate ◽  
Gene Clusters ◽  
Plant Weight ◽  
Pgp Traits ◽  
Plant Growth Promoting ◽  
Growth Promoting

Background Burkholderia sp. SSG is a bacterial endophyte isolated from boxwood leaves showing a resistant response to infection by the boxwood blight pathogen Calonectria pseudonaviculata. SSG acted as a protective and curative biocontrol agent for boxwood blight and as a bio-sanitizer of disease inoculum in the field. Many gene clusters involved in antibiotic production and plant growth promotion (PGP) were found in the genome, giving this endophyte great application potential as a treatment for plant protection. However, the PGP features have not been documented. This study investigated the plant growth promotion activity of SSG in boxwood. Methods To determine whether SSG is a plant growth promoting bacterium, four PGP traits, auxin and siderophore production, nitrogen fixation and phosphate solubilization, were examined in the laboratory with colorimetric or agar plate assays. The plant growth promoting activity of SSG was tested on three boxwood varieties characterized by slow, intermediate and fast growth rates, namely Justin Brouwers, Buddy and Winter Gem, respectively. These plants were drenched with an SSG cell suspension or water and washed plant weight was compared before and after treatment to determine growth changes after 10 months. Results The SSG culture was sustainable on nitrogen free media, suggesting that SSG may fix atmospheric nitrogen. It was also a strong phosphate solubilizer and a potent siderophore and indole-3-acetic acid (IAA) producer. Significant growth promotion was observed on boxwood cultivars Justin Brouwers, Buddy and Winter Gem 10 months after plant roots were drenched with SSG cells. The growth rate of treated plants was 76.1, 58.3, and 37.3% higher than that of the control, respectively. The degree of growth promotion was significantly different among plant varieties, notably more pronounced with the slow and intermediate growers. This study demonstrates that the SSG bacterium has multiple PGP traits and is a prospective plant biofertilizer.

scholarly journals Biological Control of Chili Damping-Off Disease, Caused by Pythium myriotylum

2021 ◽  
Vol 12 ◽  
Author(s):  
Sajjad Hyder ◽  
Amjad Shahzad Gondal ◽  
Zarrin Fatima Rizvi ◽  
Rashida Atiq ◽  
Muhammad Irtaza Sajjad Haider ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Disease Suppression ◽  
Root Weight ◽  
Dual Culture ◽  
Bacterial Isolates ◽  
Damping Off ◽  
Pythium Myriotylum ◽  
Pgp Traits

Pythium myriotylum is a notorious soil-borne oomycete that causes post-emergence damping-off in chili pepper. Of various disease management strategies, utilization of plant growth promoting rhizobacteria (PGPR) in disease suppression and plant growth promotion is an interesting strategy. The present study was performed to isolate and characterize PGPR indigenous to the chili rhizosphere in Pakistan, and to test the potential to suppress the damping-off and plant growth promotion in chili. Out of a total of 28 antagonists, eight bacterial isolates (4a2, JHL-8, JHL-12, 1C2, RH-24, 1D, 5C, and RH-87) significantly suppressed the colony growth of P. myriotylum in a dual culture experiment. All the tested bacterial isolates were characterized for biochemical attributes, and 16S rRNA sequence based phylogenetic analysis identified these isolates as Flavobacterium spp., Bacillus megaterium, Pseudomonas putida, Bacillus cereus, and Pseudomonas libanensis. All the tested bacterial isolates showed positive test results for ammonia production, starch hydrolase (except 4a2), and hydrogen cyanide production (except 4a2 and 1D). All the tested antagonists produced indole-3-acetic acid (13.4–39.0 μg mL–1), solubilized inorganic phosphate (75–103 μg mL–1), and produced siderophores (17.1–23.7%) in vitro. All the tested bacterial isolates showed varying levels of susceptibility and resistance response against different antibiotics and all these bacterial isolates were found to be non-pathogenic to chili seeds and notably enhanced percentage seed germination, plumule, redical length, and vigor index over un-inoculated control. Additionally, under pathogen pressure, bacterization increased the defense related enzymes such as Peroxidase (PO), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL) activates. Moreover, the treatment of chili seeds with these bacterial isolates significantly suppressed the damping-off caused by P. myriotylum and improved PGP traits compared to the control. In addition, a positive correlation was noticed between shoot, root length, and dry shoot and root weight, and there was a negative correlation between dry shoot, root weight, and seedling percentage mortality. These results showed that native PGPR possesses multiple traits beneficial to the chili plants and can be used to develop eco-friendly and effective seed treatment formulation as an alternative to synthetic chemical fungicides.

scholarly journals Epiphytic and endophytic bacteria that promote growth of ethnomedicinal plants in the subtropical forests of Meghalaya, India

2014 ◽  
Vol 62 (4) ◽  
pp. 1295 ◽  
Author(s):  
Fenella Mary War Nongkhla ◽  
S. R. Joshi
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Host Plants ◽  
Growth Promotion ◽  
Antagonistic Activity ◽  
Growth Promoters ◽  
Associated Bacteria ◽  
Subtropical Forests ◽  
Ethnomedicinal Plants ◽  
Sacred Forests

<p>The present study was aimed to investigate the endophytic and epiphytic bacteria associated with selected ethnomedicinal plants from the pristine subtropical forests of Meghalaya and analyse them for plant growth promotion and antagonistic ability. This study is an attempt to explore plant associated bacteria which are beneficial to host plants, and thus aid in the conservation of ethnomedicinal plants of the studied subtropical forests, which are dwindling due to exploitation. The plant growth promotion parameters like indole acetic acid (IAA) production, mineral phosphate solubilisation, acid phosphatase activity, presence of 1-aminocyclopropane-1-carboxylic acid deaminase (ACC) gene, nitrogen fixation, cellulose digestion, chitin and pectin were screened among the isolates. The study revealed significant differences in bacterial population not only between the epiphytic and endophytic microhabitats, but also amongst the host plants. Out of the 70 isolated plant associated bacteria, <em>Bacillus </em>sp<em>., Serratia </em>sp<em>., </em><em>Pseudomonas </em>sp<em>.</em><em>, Pantoea </em>sp., and <em>Lysinibacillus</em> sp. showed potent plant growth promotion properties<em>. Bacillus siamensis </em>C53 and <em>B. subtilis </em>cenB showed significant antagonistic activity against the tested pathogens. This study indicated the isolates inhabiting the plants prevalent in the subtropical sacred forests that could be explored for use as plant growth promoters while practising the cultivation and conservation of ethnomedicinal plants.</p>

scholarly journals Draft Genome Sequence of a Biocontrol Rhizobacterium, Chryseobacterium kwangjuense Strain KJ1R5, Isolated from Pepper ( Capsicum annuum )

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Jin-Ju Jeong ◽  
Hongjae Park ◽  
Byeong Hyeok Park ◽  
Mohamed Mannaa ◽  
Mee Kyung Sang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Genome Sequence ◽  
Plant Growth Promotion ◽  
Biocontrol Agent ◽  
Growth Promotion ◽  
Phytophthora Capsici ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Stress Adaptation ◽  
Phytophthora Blight

Strain KJ1R5 of the rhizobacterium Chryseobacterium kwangjuense is an effective biocontrol agent against Phytophthora blight of pepper caused by a destructive soilborne oomycete, Phytophthora capsici . Here, we present the draft genome sequence of strain KJ1R5, which contains genes related to biocontrol, plant growth promotion, and environmental stress adaptation.

scholarly journals Biological control of chili damping off disease, caused by Pythium myriotylum

2020 ◽  
Author(s):  
Sajjad Hyder ◽  
Amjad Shahzad Gondal ◽  
Zarrin Fatima Rizvi ◽  
Muhammad Irtaza Sajjad Haider ◽  
Muhammad Inam-ul-Haq
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Disease Suppression ◽  
Root Weight ◽  
Dual Culture ◽  
Bacterial Isolates ◽  
Damping Off ◽  
Pythium Myriotylum ◽  
Pgp Traits

ABSTRACTPythium myriotylum is a notorious soil-borne oomycete causes post-emergence damping off in chilli pepper. Of various disease management strategies, utilization of plant growth promoting rhizobacteria (PGPR) in disease suppression and plant growth promotion is eye catching strategy. The present study was performed to isolate and characterize PGPR indigenous to chili rhizosphere in Pakistan, and to test their potential to suppress damping off and plant growth promotion in chilli. Out of total 28 antagonists, 8 bacterial isolates (4a2, JHL-8, JHL-12, 1C2, RH-24, 1D, 5C and RH-87) significantly suppressed the colony growth of P. myriotylum in dual culture experiment. All the tested bacterial isolates were characterized for biochemical attributes, and 16S rRNA sequence based phylogenetic analysis identified these isolates as Flavobacterium spp., Bacillus megaterium, Pseudomonas putida, Bacillus cereus and Pseudomonas libanensis. All the tested bacterial isolates showed positive test results for ammonia production, starch hydrolase (except 4a2), and hydrogen cyanide production (except 4a2 and 1D). All the tested antagonists produced indole-3-acetic acid (13.4-39.0 μg ml−1), solubilized inorganic phosphate (75–103 μgml-1) and produced siderophores (17.1–23.7%) in vitro. All the tested bacterial isolates showed varied level of susceptibility and resistance response against different antibiotics and all these bacterial isolates were found non-pathogenic to chill seeds and notably enhanced percentage seed germination, plumule, redical length and vigor index over un-inoculated control. Additionally, under pathogen pressure, bacterization increased the defense related enzymes (PO, PPO and PAL) activates. Moreover, chilli seeds treatment with these bacterial isolates significantly suppressed damping-off caused by P. myriotylum, and improved PGP traits as compared to control. In addition, a positive correlation was noticed between shoot, root length and dry shoot and root weigh and a negative correlation was seen between dry shoot, root weight and seedling percentage mortality. These results showed that native PGPR possess multiple traits beneficial to the chilli plants and can be used to develop eco-friendly and effective seed treatment formulation as an alternative to synthetic chemical fungicides.

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