scholarly journals The Melon Zym Locus Conferring Resistance to ZYMV: High Resolution Mapping and Candidate Gene Identification

Agronomy ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 2427
Author(s):  
Nastacia Adler-Berke ◽  
Yitzchak Goldenberg ◽  
Yariv Brotman ◽  
Irina Kovalski ◽  
Amit Gal-On ◽  
...  
Keyword(s):  
High Resolution ◽  
Resistance Gene ◽  
Genetic Map ◽  
Positional Cloning ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Susceptible Genotype ◽  
Bac Clone ◽  
Resistant Parent ◽  
Viral Spread

Zucchini yellow mosaic virus (ZYMV; potyviridae) represents a major pathogen of Cucurbitaceae crops. ZYMV resistance in melon PI 414723 is conditioned by a dominant allele at the Zym locus. This resistant accession restricts viral spread and does not develop mosaic symptoms, but necrosis sometimes develops in response to inoculation. In previous studies, Zym has been mapped to linkage group II of the melon genetic map. In the present study, positional cloning of the locus was undertaken, starting from the CM-AG36 SSR marker at approximately 2 cm distance. We utilized five mapping populations that share the same resistant parent, PI 414723, and analyzed a total of 1630 offspring, to construct a high-resolution genetic map of the Zym locus. Two melon BAC libraries were used for chromosome walking and for developing new markers closer to the resistance gene by BAC-end sequencing. A BAC contig was constructed, and we identified a single BAC clone, from the ZYMV susceptible genotype MR-1, that physically encompasses the resistance gene. A second clone was isolated from another susceptible genotype, WMR 29, and the two clones were fully sequenced and annotated. Additional markers derived from the sequenced region delimited the region to 17.6 kb of a sequence that harbors a NAC-like transcription factor and, depending on the genotype, either two or three R-gene homologs with a CC-NBS-LRR structure. Mapping was confirmed by saturating the map with SNP markers using a single mapping population. The same region was amplified and sequenced also in the ZYMV resistant genotype PI 414723. Because numerous polymorphic sites were noted between genotypes, we could not associate resistance with a specific DNA polymorphism; however, this study enables molecular identification of Zym and paves the way to functional studies of this important locus.

scholarly journals Identification and Utility of Markers Linked to the Zucchini Yellow Mosaic Virus Resistance Gene in Watermelon

2009 ◽  
Vol 134 (5) ◽  
pp. 529-534 ◽  
Author(s):  
Karen R. Harris ◽  
Kai-Shu Ling ◽  
W. Patrick Wechter ◽  
Amnon Levi
Keyword(s):  
United States ◽  
Genetic Distance ◽  
Mosaic Virus ◽  
Resistance Gene ◽  
Zucchini Yellow Mosaic Virus ◽  
Recessive Gene ◽  
The United States ◽  
Yellow Mosaic Virus ◽  
Recurrent Parent ◽  
Resistant Parent

Zucchini yellow mosaic virus (ZYMV) is one of the most economically important viruses affecting watermelon [Citrullus lanatus (Thunb.) Matsun & Nakai var. lanatus] in the United States. The ZYMV-Florida strain (ZYMV-FL) is considered a major limitation to commercial watermelon production in the United States. Inheritance of resistance to ZYMV-FL is conferred by a recessive gene. This report describes the identification of single-reaction, polymerase chain reaction-based markers linked to the ZYMV-FL resistance gene in watermelon. In this study, we identified a marker ZYMV-resistant polymorphism (ZYRP) linked to the ZYMV-FL resistance gene locus (genetic distance of 8 cM) in an F2 population, and in a backcross one to the resistant parent population (BC1R) (genetic distance of 13 cM). The identification of a single nucleotide polymorphism within the ZYRP marker for the parental genotypes allowed the development of a sequence-characterized amplification region marker linked to the ZYMV-FL resistance gene. Experiments using a BC2F2 population derived from the U.S. Plant Introduction 595203 (C. lanatus var. lanatus) and the recurrent parent ‘Charleston Gray’ indicated that the ZYRP marker can be used in marker-assisted selection to identify genotypes containing the gene conferring ZYMV-FL resistance in watermelon.

scholarly journals Evaluation of ‘AU-Performance’ Watermelon for Its Response to Virus Inoculation

2009 ◽  
Vol 19 (3) ◽  
pp. 609-612 ◽  
Author(s):  
John F. Murphy ◽  
Fenny Dane
Keyword(s):  
Mosaic Virus ◽  
Fungal Pathogens ◽  
Citrullus Lanatus ◽  
Zucchini Yellow Mosaic Virus ◽  
Systemic Infection ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Susceptible Parent ◽  
Resistant Parent ◽  
Systemic Symptoms

The watermelon (Citrullus lanatus var. lanatus) ‘AU-Performance’ was developed for resistance to multiple fungal pathogens and the plant virus, zucchini yellow mosaic virus (ZYMV). A greenhouse-based evaluation was carried out to determine the response of ‘AU-Performance’ to inoculation with three important cucurbit (Cucurbitaceae) viruses in the genus Potyvirus: papaya ringspot virus (PRSV), watermelon mosaic virus (WMV), and ZYMV. The evaluation included the resistant parent (PI595203), the susceptible parent (‘AU-Producer’), and varieties AU-Allsweet and Charleston Gray. Each of the three viruses systemically infected ‘AU-Performance’ with 100% infection and development of characteristic systemic symptoms. The susceptible parent (‘AU-Producer’), ‘AU-Allsweet’, and ‘Charleston Gray’ responded similarly with 100% infection and systemic symptoms. In contrast, the resistant parent (PI595203) was resistant to WMV and ZYMV; however, PRSV-inoculated plants developed a systemic infection with accompanied symptoms and high levels of PRSV accumulation in noninoculated leaves. PI595203 was shown in previous studies to be resistant to PRSV. We show in this report that under greenhouse conditions and application of virus by mechanical inoculation, ‘AU-Performance’ was not resistant to infection by the three potyviruses.

Exploitation of Arabidopsis-tomato synteny to construct a high-resolution map of the ovate-containing region in tomato chromosome 2

Genome ◽  
2001 ◽  
Vol 44 (3) ◽  
pp. 470-475 ◽  
Author(s):  
Hsin-Mei Ku ◽  
Jiping Liu ◽  
Sami Doganlar ◽  
Steven D Tanksley
Keyword(s):  
High Resolution ◽  
Fruit Development ◽  
Positional Cloning ◽  
Fruit Shape ◽  
Chromosome 2 ◽  
Bac Clone ◽  
Bac Clones ◽  
Tomato Chromosome ◽  
High Resolution Map ◽  
Shape Characteristics

High-resolution genetic and physical maps were constructed for the region of chromosome 2 containing the major fruit-shape locus ovate. A total of 3000 NIL F2 and F3 NILs derived from Lycopersicon esculentum cv. Yellow Pear (TA503) × L. pennellii (a wild tomato) were used to position ovate adjacent to the marker TG645 and flanked by markers TX700 and BA10R (a 0.03-cM interval). BAC libraries and a BIBAC library were screened with the closest marker, TG645. Genetic mapping with the ends of isolated BAC clones revealed that two BAC clones (100 and 140 kb) both contained the ovate locus. Screening of sequences from these BAC clones revealed synteny between this segment of tomato chromosome 2 and the chromosome-4 region of Arabidopsis containing the BAC clone ATAP22. Microsynteny between the two genomes was exploited to find additional markers near the ovate locus. The placement of ovate on a BAC clone will now allow cloning of this locus and, hence, may open the door to understanding the molecular basis of fruit development and also facilitate the genetic engineering of fruit-shape characteristics. This also represents the first time that microsynteny with Arabidopsis has been exploited for positional cloning purposes in a different plant family.Key words: map, fruit development, colinearity, positional cloning.

High-resolution mapping of zym, a recessive gene for Zucchini yellow mosaic virus resistance in cucumber

2013 ◽  
Vol 126 (12) ◽  
pp. 2983-2993 ◽  
Author(s):  
Masashi Amano ◽  
Akira Mochizuki ◽  
Yumi Kawagoe ◽  
Keisuke Iwahori ◽  
Kaori Niwa ◽  
...  
Keyword(s):  
High Resolution ◽  
Mosaic Virus ◽  
Virus Resistance ◽  
Zucchini Yellow Mosaic Virus ◽  
Recessive Gene ◽  
Yellow Mosaic Virus ◽  
High Resolution Mapping ◽  
Resolution Mapping

scholarly journals High Prevalence of Three Potyviruses Infecting Cucurbits in Oklahoma and Phylogenetic Analysis of Cucurbit Aphid-Borne Yellows Virus Isolated from Pumpkins

Pathogens ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 53
Author(s):  
Vivek Khanal ◽  
Harrington Wells ◽  
Akhtar Ali
Keyword(s):  
Phylogenetic Analysis ◽  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
High Prevalence ◽  
The Us ◽  
Close Relationship ◽  
The Status ◽  
Low Incidence

Field information about viruses infecting crops is fundamental for understanding the severity of the effects they cause in plants. To determine the status of cucurbit viruses, surveys were conducted for three consecutive years (2016–2018) in different agricultural districts of Oklahoma. A total of 1331 leaf samples from >90 fields were randomly collected from both symptomatic and asymptomatic cucurbit plants across 11 counties. All samples were tested with the dot-immunobinding assay (DIBA) against the antisera of 10 known viruses. Samples infected with papaya ringspot virus (PRSV-W), watermelon mosaic virus (WMV), zucchini yellow mosaic virus (ZYMV), and cucurbit aphid-borne-yellows virus (CABYV) were also tested by RT-PCR. Of the 10 viruses, PRSV-W was the most widespread, with an overall prevalence of 59.1%, present in all 11 counties, followed by ZYMV (27.6%), in 10 counties, and WMV (20.7%), in seven counties, while the remaining viruses were present sporadically with low incidence. Approximately 42% of the infected samples were positive, with more than one virus indicating a high proportion of mixed infections. CABYV was detected for the first time in Oklahoma, and the phylogenetic analysis of the first complete genome sequence of a CABYV isolate (BL-4) from the US showed a close relationship with Asian isolates.

High-resolution genetic map and YAC contig around the mouse neurological locus reeler

1994 ◽  
Vol 5 (12) ◽  
pp. 756-761 ◽  
Author(s):  
J. C. Montgomery ◽  
M. H. Guarn�eri ◽  
K. E. Tartaglia ◽  
L. A. Flaherty
Keyword(s):  
High Resolution ◽  
Genetic Map ◽  
Yac Contig

scholarly journals Increase in Zucchini yellow mosaic virus Symptom Severity in Tolerant Zucchini Cultivars Is Related to a Point Mutation in P3 Protein and Is Associated with a Loss of Relative Fitness on Susceptible Plants

2003 ◽  
Vol 93 (12) ◽  
pp. 1478-1484 ◽  
Author(s):  
C. Desbiez ◽  
A. Gal-On ◽  
M. Girard ◽  
C. Wipf-Scheibel ◽  
H. Lecoq
Keyword(s):  
Mosaic Virus ◽  
Point Mutation ◽  
Zucchini Yellow Mosaic Virus ◽  
Genetic Load ◽  
Yellow Mosaic Virus ◽  
Relative Fitness ◽  
Interspecific Crosses ◽  
Mixed Infections ◽  
Wild Type ◽  
Sequence Comparisons

Zucchini yellow mosaic virus (ZYMV, Potyvirus) is a very damaging cucurbit virus worldwide. Interspecific crosses with resistant Cucurbita moschata have led to the release of “resistant” zucchini squash (C. pepo) F1 hybrids. However, although the resistance is almost complete in C. moschata, the commercial C. pepo hybrids are only tolerant. ZYMV evolution toward increased aggressiveness on tolerant hybrids was observed in the field and was obtained experimentally. Sequence comparisons and recombination experiments revealed that a point mutation in the P3 protein of ZYMV was enough to induce tolerance breaking. Competition experiments were performed between quasi-isogenic wild-type, and aggressive variants of ZYMV distinguished by monoclonal antibodies. The aggressive mutants were more fit than wild-type strains in mixed infections of tolerant zucchini, but they presented a drastic fitness loss in mixed infections of susceptible zucchini or melon. Thus, the ability to induce severe symptoms in tolerant zucchini is related to a genetic load in susceptible zucchini, but also on other susceptible hosts. This represents the first quantitative study of the fitness cost associated with tolerance breaking for a plant virus. Thus, although easily broken, the tolerance might prove durable in some conditions if the aggressive variants are counterselected in susceptible crops.

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