scholarly journals Use of GnRH for Synchronization of the Follicular Wave in Assisted Reproductive Technologies in Sheep: A Preliminary Study

Animals ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1208
Author(s):  
Aina Año-Perello ◽  
Zurisaday Santos-Jimenez ◽  
Teresa Encinas ◽  
Paula Martinez-Ros ◽  
Antonio Gonzalez-Bulnes
Keyword(s):  
Luteinizing Hormone ◽  
Assisted Reproductive Technologies ◽  
Ovarian Response ◽  
Reproductive Technologies ◽  
Estrus Synchronization ◽  
Short Term ◽  
Follicular Wave ◽  
Preliminary Study ◽  
Cost Efficient ◽  
Set Up

The present study aimed to set up a short-term protocol for synchronization of follicular wave emergence in sheep, concomitant with estrus synchronization, which would improve ovarian response in assisted reproductive technologies. Administration of a single GnRH dose, concomitant with the insertion of a progesterone-loaded CIDR device, caused regression of gonadotrophin-dependent follicles ≥4 mm in all the GnRH-treated sheep and in around 80% of the controls treated only with CIDR (p < 0.05). Similar percentages of ewes lost all follicles (around 70%) or only the largest one (around 30%) in both groups. Hence, 54.1% and 70% of the sheep lost all large follicles and initiated a new follicular wave in the control and GnRH groups, respectively (p < 0.05). The remaining sheep showed follicles that were still not dependent of luteinizing hormone (LH). So, in fact, all the sheep had non-dominant follicles after treatment. In conclusion, a treatment including GnRH at CIDR insertion would offer a time- and cost-efficient protocol for inducing follicular turnover and synchronizing a new follicular wave at any stage of the estrous cycle.

The role of maternal age in the assisted reproductive technologies

1999 ◽  
Vol 7 (1) ◽  
pp. 41-60 ◽  
Author(s):  
Mark A Damario ◽  
Owen K Davis ◽  
Zev Rosenwaks
Keyword(s):  
Older Women ◽  
Assisted Reproductive Technologies ◽  
Clinical Importance ◽  
Ovarian Response ◽  
Reproductive Technologies ◽  
Abortion Rate ◽  
Western World ◽  
Delayed Childbearing ◽  
Art Outcome

Age is perhaps the most important single variable influencing outcome in the assisted reproductive technologies (ART). The effect of advancing age on clinical ART outcome is manifested not only in the pattern of ovarian response to stimulation regimens, but also in reduced implantation efficiency and an increased spontaneous abortion rate. The clinical importance of these factors is compounded by the fact that increasing numbers of older women are presenting for ART treatment. Delayed childbearing is becoming increasingly common in the western world. The availability of methods of birth control, educational and career priorities for women, and the increased rates of divorce and remarriage are some of the factors contributing to this phenomenon.

Genetic polymorphisms of reproductive hormones and their receptors in assisted reproduction technology for patients with polycystic ovary syndrome

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Yulia A. Koloda ◽  
Yulia V. Denisova ◽  
Natalia M. Podzolkova
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Assisted Reproductive Technologies ◽  
Polycystic Ovary ◽  
Ovarian Response ◽  
Reproductive Technologies ◽  
Reproductive Hormones ◽  
Current Data ◽  
Nucleotide Polymorphisms ◽  
Ovary Syndrome ◽  
Art Programs

Abstract Polycystic ovary syndrome (PCOS) is one of the most common endocrinopathies in women of childbearing, which is defined by the accumulation of multiple, small fluid-filled ovarian cysts without the selection of a single dominant follicle. Most PCOS phenotypes are characterized by the absence of spontaneous ovulation, resistance toward ovulation inductors, the production of a large immature oocytes number, and the high prevalence of ovarian hyperstimulation syndrome, resulting in reduced assisted reproductive technologies (ART) programs effectiveness. The review analyses current data about the relationship between polymorphism genotypes of KISS genes, follicle stimulating hormone (FSH), luteinizing hormone (LH), anti-Müllerian hormone (AMH) and their receptors genes, gonadotropin-releasing hormone (GnRH), estrogen, and progesterone receptors genes, the PCOS risk and the features of ovarian response to stimulation during ART cycles. The use of single nucleotide polymorphisms (SNPs) as prognostic markers of ART programs outcomes would provide a personalized approach to the drugs and doses choice for ovarian stimulation and significantly increase the chance of pregnancy.

418 EFFECT OF SUPEROVULATION INITIATED AT FOLLICULAR WAVE EMERGENCE IN Santa Inês EWES

2010 ◽  
Vol 22 (1) ◽  
pp. 366
Author(s):  
B. D. M. Silva ◽  
T. A. S. N. Silva ◽  
L. Dalcin ◽  
C. M. Lucci ◽  
J. P. Neves
Keyword(s):  
Total Dose ◽  
Buenos Aires ◽  
Ovarian Response ◽  
Corpora Lutea ◽  
Dominant Follicle ◽  
Treatment Groups ◽  
Follicular Wave ◽  
Preliminary Study ◽  
Santa Inês ◽  
Fresh Semen

Superovulation in ewes has been a source of many studies aimed at obtaining high superovulatory response and number of viable embryos recovered. In a protocol called Day 0, superovulatory treatment was initiated at the time of wave emergence in the absence of a dominant follicle (Menchaca A et al. 2002 Theriogenology 58, 1713-1721). The aim of this study was to compare ovarian response and number of embryos recovered after treatment between ewes treated with a Day 0 protocol and those treated with a traditional protocol. Santa InÊs ewes (n= 18) between 2 and 5 years old were randomly distributed into 2 superovulation treatment groups: traditional protocol and Day 0 protocol. Each treatment was repeated twice in a crossover model. The traditional protocol consisted of the intravaginal insertion of a sponge containing 60 mg of medroxiprogesterone acetate (MAP; Progespon®, Tecnopec, São Paulo, Brazil) for 14 days, which was replaced on Day 7, followed by 150 μg of cloprostenol (Prolise®, Tecnopec) i.m. On Day 12, FSH (Folltropin®-V, Bioniche, Belleville, Ontario, Canada) treatment was initiated using a total dose of 200 mg, given in twice-daily i.m. injections that decreased in dose over 4 days. A dose of 200 IU of eCG (Novormon®, Syntex, Buenos Aires, Argentina) i.m. was given at the time of sponge withdrawal. The AI was done by laparoscopy at 48 and 55 h after sponge withdrawal using fresh semen. The Day 0 protocol consisted of a previous 9-day synchronization treatment with a sponge containing 60 mg of MAP, followed by 150 μ of cloprostenol i.m. and 200 IU of eCG i.m. given on Day 7. A dose of 0.05 mg of GnRH (lecirelin; Gestran Plus®, Tecnopec) i.m. was given 16 h after sponge withdrawal. In a preliminary study, 38 ewes ovulated 42 ± 52 h after sponge withdrawal. Therefore, 48 h after sponge removal was considered as Day 0 and FSH treatment was initiated at that time, with a total dose of 200 mg of Folltropin®-V, given in 6 twice-daily decreasing doses. Two doses of cloprostenol (150 μg) were given i.m. concurrent with the fifth and sixth FSH treatments. Gonadotropin-releasing hormone (0.05 mg of lecirelin i.m.) was given 12 h after the last FSH treatment. Artificial insemination with fresh semen was done by laparoscopy 16 and 26 h after GnRH treatment. Five days after AI, embryos were recovered surgically. Results were evaluated by the parametric t-test. The number of corpora lutea and ova/embryos recovered did not differ (P > 0.05) between the traditional (9.8 ± 5.3; 4.5 ± 4.6) and Day 0 (10.0 ± 6.0; 3.5 ± 4.3) protocols. Similarly, no difference in the number of viable embryos was observed between treatments (1.6 ± 2.0 and 1.7 ± 2.4 for the traditional and Day 0 treatments, respectively). Within viable embryos, the traditional protocol (0.4 ± 1.0) resulted in ahigher (P < 0.05) number of morulae than that of the Day 0 protocol (0.1 ± 0.3). The ewes that had no superovulatory response did not differ (P > 0.05) between the traditional (11.11%) and Day 0 (5.56%) protocols. In summary, there was no difference in the parameters evaluated between both protocols. Financial support: FAPDF, CNPq, Tecnopec.

scholarly journals Endometriosis-Related Infertility: The Role of the Assisted Reproductive Technologies

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Eric S. Surrey
Keyword(s):  
Assisted Reproductive Technologies ◽  
Large Body ◽  
Ovarian Response ◽  
Reproductive Technologies ◽  
Invasive Disease ◽  
Definitive Treatment ◽  
Success Rates ◽  
Vitro Fertilization ◽  
Ovarian Endometriomas

The assisted reproductive technologies, particularly in vitro fertilization (IVF), represent the most efficient and successful means of overcoming infertility associated with endometriosis. Although older studies suggest that IVF outcomes are compromised in endometriosis patients, more contemporary reports show no differences compared to controls. The exception may be evidence of poorer outcomes and diminished ovarian response in women with advanced disease, particularly those with significant ovarian involvement or prior ovarian surgery. Prolonged pre-IVF cycle suppressive medical therapy, particularly gonadotropin releasing hormone agonists, appears to improve success rates in a subset of endometriosis patients. However, as of yet, there is no diagnostic marker to specifically identify those who would most benefit from this approach. Pre-IVF cycle surgical resection of nonovarian disease has not been consistently shown to improve outcomes with the possible exception of resection of deeply invasive disease, although the data is limited. Precycle resection of ovarian endometriomas does not have benefit and should only be performed for gynecologic indications. Indeed, there is a large body of evidence to suggest that this procedure may have a deleterious impact on ovarian reserve and response. A dearth of appropriately designed trials makes development of definitive treatment paradigms challenging.

scholarly journals ENDOMETRIOSIS AND INFERTILITY. OPERATION OR ASSISTED REPRODUCTIVE TECHNOLOGIES?

2018 ◽  
Vol 5 (1) ◽  
pp. 31-36
Author(s):  
Natalya S. Kuzmina ◽  
V. F Bezhenar ◽  
A. S Kalugina
Keyword(s):  
Surgical Treatment ◽  
Ovarian Reserve ◽  
Assisted Reproductive Technologies ◽  
Transvaginal Ultrasound ◽  
Statistical Significance ◽  
Reproductive Function ◽  
Ovarian Response ◽  
Reproductive Technologies ◽  
Ovarian Endometrioma ◽  
Infertility Patients

The aim of the study was to study the influence of the ovarian endometrioma on the ovarian reserve, the ovarian response in ovarian hyperstimulation, and outcomes of assisted reproductive technologies (ART). Material and methods. The study included 43 infertility patients underwent the surgical treatment of an ovarian endometrioma in the history. During the study, the follicles were counted separately (according to transvaginal ultrasound), eggs and embryos were obtained from the operated and intact ovaries. Results. The number of follicles in the ovary, operated due to endometriosis, is significantly lower than in the contralateral ovary (p = 0.005). The number of ovules punctured from the ovary, operated for endometriosis, is less than the number of ovules obtained from the intact ovary, but the difference does not reach statistical significance (p = 0.07). The number of high-quality embryos obtained from the ovary, operated for endometrioma, is statistically significantly lower than the number of similar embryos obtained from the intact ovary (p = 0.013). Conclusion. According to the conducted study, it can be concluded that the surgical treatment of endometrioma in infertility patients with the need for the implementation of the reproductive function reduces the number of follicles in the operated ovary and, accordingly, does not lead to an improvement in indices of the infertility treatment using ART. At the same time, the very presence of endometrioma is also known to negatively impact on outcomes of ART. Therefore, in infertility patients with the reduced ovarian reserve, individualization of approaches to treatment is extremely important.

Estrous Cycle of Induced Ovulators: Lesson From The Camel — A Review

2018 ◽  
Vol 1 (2) ◽  
pp. 108-125
Author(s):  
Y Dauda
Keyword(s):  
Estrous Cycle ◽  
Ovarian Function ◽  
Assisted Reproductive Technologies ◽  
Current Knowledge ◽  
Reproductive Technologies ◽  
Wave Pattern ◽  
Follicular Wave ◽  
Calving Intervals ◽  
Camel Husbandry ◽  
Ovulatory Process

Camel, rabbit, cat, ferrets, minks, koala and meadow moles are induced ovulators requiring copulation to trigger the ovulatory process and the estrous cycle differs from that of other domestic animals. The estrous cycle in these animals composed of follicular recruitment, follicular growth, follicular maturity and follicular regression phase. These animals are variously reared as companion, fur-bearing and meat animals. Among these, the camel is the most valuable and classical induced ovulator which is rear not only for milk and meat, but as work animal and contributes effectively to the welfare of people in harsh and difficult environments. As a classical induced ovulatory, camelid has cycling receptivity with distinctive estrus but requires mating in order to ovulate. The other classes of induced ovulators like cats and ferrets require both the presence of male to achieve behavioral estrus and actual copulation to ovulate. The camel has good prospects of survival as a suitable livestock for projects of sustainable agriculture and animal production under harsh desert or arid conditions. However the reproductive nature of camels presents a huge challenge to camel husbandry. The natural constraints include the long period of attaining puberty, limited breeding season, difficulties in induced ovulation, long gestation period and inter-calving intervals. Efforts to improve the reproductive efficiency of the female camel are closely related to a better understanding of the folliculogenesis or follicular wave pattern. Many investigators might not be aware of the peculiar reproductive information available about this animal species. A working knowledge of ovarian function or estrous cycle will be of immense importance to the application of assisted reproductive technologies (ARTs) and enhancements of reproduction in camelids. This work presents the overview of estrous cycle in camel as a classical example of induced ovulators with the aim of providing current knowledge to the reader and to stimulate wider research interest in camel research and reproduction.

scholarly journals Equine Chorionic Gonadotropin as an Effective FSH Replacement for In Vitro Ovine Follicle and Oocyte Development

2021 ◽  
Vol 22 (22) ◽  
pp. 12422
Author(s):  
Chiara Di Berardino ◽  
Alessia Peserico ◽  
Giulia Capacchietti ◽  
Martina Crociati ◽  
Maurizio Monaci ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Oocyte Development ◽  
Cultural Conditions ◽  
Low Costs ◽  
Equine Chorionic Gonadotropin ◽  
Set Up ◽  
Positive Effect

The use of assisted reproductive technologies (ART) still requires strategies through which to maximize individual fertility chances. In vitro folliculogenesis (ivF) may represent a valid option to convey the large source of immature oocytes in ART. Several efforts have been made to set up ivF cultural protocols in medium-sized mammals, starting with the identification of the most suitable gonadotropic stimulus. In this study, Equine Chorionic Gonadotropin (eCG) is proposed as an alternative to Follicle Stimulating Hormone (FSH) based on its long superovulation use, trans-species validation, long half-life, and low costs. The use of 3D ivF on single-ovine preantral (PA) follicles allowed us to compare the hormonal effects and to validate their influence under two different cultural conditions. The use of eCG helped to stimulate the in vitro growth of ovine PA follicles by maximizing its influence under FBS-free medium. Higher performance of follicular growth, antrum formation, steroidogenic activity and gap junction marker expression were recorded. In addition, eCG, promoted a positive effect on the germinal compartment, leading to a higher incidence of meiotic competent oocytes. These findings should help to widen the use of eCG to ivF as a valid and largely available hormonal support enabling a synchronized in vitro follicle and oocyte development.

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