Evaluation of an Acute Osmotic Stress in European Sea Bass via Skin Mucus Biomarkers

European sea bass is a marine teleost which can inhabit a broad range of environmental salinities. So far, no research has studied the physiological response of this fish to salinity challenges using modifications in skin mucus as a potential biological matrix. Here, we used a skin mucus sampling technique to evaluate the response of sea bass to several acute osmotic challenges (for 3 h) from seawater (35‰) to two hypoosmotic environments, diluted brackish water (3‰) and estuarine waters (12‰), and to one hyperosmotic condition (50‰). For this, we recorded the volume of mucus exuded and compared the main stress-related biomarkers and osmosis-related parameters in skin mucus and plasma. Sea bass exuded the greatest volume of skin mucus with the highest total contents of cortisol, glucose, and protein under hypersalinity. This indicates an exacerbated acute stress response with possible energy losses if the condition is sustained over time. Under hyposalinity, the response depended on the magnitude of the osmotic change: shifting to 3‰ was an extreme salinity change, which affected fish aerobic metabolism by acutely modifying lactate exudation. All these data enhance the current scarce knowledge of skin mucus as a target through which to study environmental changes and fish status.

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Water temperature modifies the acute stress response of European sea bass, Dicentrarchus labrax L. (1758)

Journal of Thermal Biology ◽

10.1016/j.jtherbio.2018.09.006 ◽

2018 ◽

Vol 78 ◽

pp. 84-91 ◽

Cited By ~ 3

Author(s):

Athanasios Samaras ◽

Nikos Papandroulakis ◽

Konstadia Lika ◽

Michail Pavlidis

Keyword(s):

Stress Response ◽

Water Temperature ◽

Acute Stress ◽

Dicentrarchus Labrax ◽

Sea Bass ◽

European Sea Bass ◽

Acute Stress Response

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Acute stress response of European sea bass Dicentrarchus labrax under blue and white light

Aquaculture ◽

10.1016/j.aquaculture.2012.07.033 ◽

2012 ◽

Vol 364-365 ◽

pp. 48-52 ◽

Cited By ~ 10

Author(s):

Nafsika Karakatsouli ◽

Panagiotis Katsakoulis ◽

Georgios Leondaritis ◽

Dimitris Kalogiannis ◽

Sofronios E. Papoutsoglou ◽

...

Keyword(s):

Stress Response ◽

White Light ◽

Acute Stress ◽

Dicentrarchus Labrax ◽

Sea Bass ◽

European Sea Bass ◽

Acute Stress Response

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Early life low intensity stress experience modifies acute stress effects on juvenile brain cell proliferation of European sea bass ( D. Labrax )

Behavioural Brain Research ◽

10.1016/j.bbr.2016.09.026 ◽

2017 ◽

Vol 317 ◽

pp. 109-121 ◽

Cited By ~ 7

Author(s):

S. Fokos ◽

M. Pavlidis ◽

T. Yiotis ◽

A. Tsalafouta ◽

N. Papandroulakis ◽

...

Keyword(s):

Cell Proliferation ◽

Early Life ◽

Acute Stress ◽

Sea Bass ◽

Brain Cell ◽

European Sea Bass ◽

Stress Effects ◽

Low Intensity ◽

Stress Experience ◽

Intensity Stress

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Effect of Light–Dark Cycle on Skin Mucosal Immune Activities of Gilthead Seabream (Sparus aurata) and European Sea Bass (Dicentrarchus labrax)

Fishes ◽

10.3390/fishes5010010 ◽

2020 ◽

Vol 5 (1) ◽

pp. 10

Author(s):

Diana Ceballos-Francisco ◽

Alberto Cuesta ◽

María Ángeles Esteban

Keyword(s):

Bactericidal Activity ◽

Sparus Aurata ◽

Dicentrarchus Labrax ◽

Maximum Level ◽

Sea Bass ◽

Immunoglobulin M ◽

Gilthead Seabream ◽

Dark Cycle ◽

European Sea Bass ◽

Skin Mucus

Changes in different immune activities in the skin mucus of gilthead seabream (Sparus aurata L.) and European sea bass (Dicentrarchus labrax L.) specimens exposed to a constant light–dark photoperiod (12 h L:12 h D) were studied. Samples were collected at 08:00 (light on), 14:00, 20:00 (light off), 02:00, and again at 08:00 to determine immunoglobulin M (IgM) levels, several enzymes related to the immune system, and bactericidal activity. IgM levels were higher during the day in seabream and reached a minimum value at 20:00, but it was hardly affected in sea bass. No significant variations were recorded in the levels of protease and antiprotease. Peroxidase reached its maximum level in seabream at 02:00, the same time that it reached its minimum level in sea bass. Lysozyme showed little variation in seabream, but it was significantly lower at 14:00 than during the rest of the cycle in sea bass. Finally, different interspecific variations on bactericidal activity against Vibrio harveyi were recorded. The findings demonstrate that the immune parameters present in skin mucus of these important fish species are affected by the light–dark cycle and that there are substantial interspecies differences.

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Physiological responses of European sea bass Dicentrarchus labrax to different stocking densities and acute stress challenge

Aquaculture ◽

10.1016/j.aquaculture.2007.12.012 ◽

2008 ◽

Vol 275 (1-4) ◽

pp. 319-328 ◽

Cited By ~ 109

Author(s):

P. Di Marco ◽

A. Priori ◽

M.G. Finoia ◽

A. Massari ◽

A. Mandich ◽

...

Keyword(s):

Acute Stress ◽

Physiological Responses ◽

Dicentrarchus Labrax ◽

Sea Bass ◽

European Sea Bass

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Evaluation of behavioral changes induced by a first step of domestication or selection for growth in the European sea bass (Dicentrarchus labrax): A self-feeding approach under repeated acute stress

Aquaculture ◽

10.1016/j.aquaculture.2010.04.027 ◽

2010 ◽

Vol 306 (1-4) ◽

pp. 211-217 ◽

Cited By ~ 30

Author(s):

S. Millot ◽

S. Péan ◽

D. Leguay ◽

A. Vergnet ◽

B. Chatain ◽

...

Keyword(s):

Acute Stress ◽

Dicentrarchus Labrax ◽

Sea Bass ◽

Behavioral Changes ◽

European Sea Bass ◽

Selection For

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Evaluation of the Inclusion of the Green Seaweed Ulva ohnoi as an Ingredient in Feeds for Gilthead Sea Bream (Sparus aurata) and European Sea Bass (Dicentrarchus labrax)

Animals ◽

10.3390/ani11061684 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1684

Author(s):

Francisca P. Martínez-Antequera ◽

Juan A. Martos-Sitcha ◽

Jose M. Reyna ◽

Francisco J. Moyano

Keyword(s):

Sparus Aurata ◽

Dicentrarchus Labrax ◽

Sea Bass ◽

Positive Influence ◽

Sea Bream ◽

European Sea Bass ◽

Skin Mucus ◽

Negative Effect ◽

Green Seaweed ◽

Ulva Ohnoi

This study evaluated the use of Ulva ohnoi as an ingredient in feeds for aquaculture in three different experiments. Experiment 1 was oriented to confirm the negative effect of U. ohnoi on fish digestion. Experiment 2 assessed the effect on growth, feed efficiency, and immune status of juvenile sea bass (Dicentrarchus labrax) fed on diets including U. ohnoi, previously treated or not with carbohydrases used to partially hydrolyze indigestible polysaccharides. Experiment 3 was aimed to evaluate the potential protective effect of U. ohnoi on the oxidative status of sea bream (Sparus aurata) challenged by the consumption of a feed formulated with the oil fraction completely oxidized. Results show a negligible effect of U. ohnoi meal on protein digestion when included in feeds at levels of 10% or less. Moreover, results of growth and feed use evidenced the possibility of using up to 5% inclusion of algal meal in feeds without adverse effects on the zootechnical parameters, while the enzyme pretreatment was ineffective to improve its nutritional use. Finally, the inclusion of U. onhoi in feeds determined both an immunostimulatory effect, evidenced by an increase in skin mucus lysozyme in the two mentioned fish species, and a positive influence on the oxidative metabolism of seabream when fed on a diet including rancid oil.

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Skin mucus proteome map of European sea bass (Dicentrarchus labrax)

PROTEOMICS ◽

10.1002/pmic.201500120 ◽

2015 ◽

Vol 15 (23-24) ◽

pp. 4007-4020 ◽

Cited By ~ 46

Author(s):

Héctor Cordero ◽

Monica F. Brinchmann ◽

Alberto Cuesta ◽

José Meseguer ◽

María A. Esteban

Keyword(s):

Dicentrarchus Labrax ◽

Sea Bass ◽

European Sea Bass ◽

Skin Mucus

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Genome-wide epigenomic stress response in the European sea bass (Dicentrarchus labrax, L.)

10.21203/rs.3.rs-48905/v2 ◽

2020 ◽

Author(s):

Madoka V. KRICK ◽

Erick DESMARAIS ◽

Athanasios SAMARAS ◽

Elise GUERET ◽

Arkadios DIMITROGLOU ◽

...

Keyword(s):

Stress Response ◽

Cytosine Methylation ◽

Acute Stress ◽

Dicentrarchus Labrax ◽

Sea Bass ◽

Blood Parameter ◽

European Sea Bass ◽

Genome Wide ◽

Stress Related Genes ◽

The Brain

Abstract Background: While the stress response inspired genome-wide epigenetic studies in vertebrate models, it remains mostly ignored in fish. We modified the epiGBS (epiGenotyping By sequencing) technique to explore changes in genome-wide cytosine methylation to a repeated acute stress challenge in the nucleated red blood cells (RBCs) of the European sea bass (Dicentrarchus labrax). This species is widely studied in both the natural and farmed environments, including issues regarding health and welfare.Results: We retrieved 501,108,033 sequencing reads after trimming, with a mean mapping efficiency of 73.0% (unique best hits). Minor changes in RBC methylome appear to manifest after the stress challenge. Only, fifty-seven differentially methylated cytosines (DMCs) close to 51 distinct stress-related genes distributed on 17 of 24 linkage groups (LGs) were detected between RBCs of pre- and post-stress individuals. However, literature surveys indicated that 38 of these genes were previously reported as differentially expressed in the brain of zebrafish, most of them involved in stress coping differences. DMC-related genes associated to the Brain Derived Neurotrophic Factor, a protein that favors stress adaptation and fear memory, appear especially relevant to integrate a centrally produced stress response.Conclusion: By putting forward DMCs associated to stress-related genes, we show that minimally invasive RBCs deserve more attention to investigate the epigenetic response to stress and components of the stress response without sacrificing fish. In parallel to blood parameter measurements (e.g. cortisol, glucose levels), and other molecular approaches (e.g. gene expression variation), features of the epigenetic landscape may offer new opportunities for biomonitoring components of the stress response in fish.

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Genome-Wide Epigenomic Stress Response in the European Sea Bass (Dicentrarchus Labrax, L.)

10.21203/rs.3.rs-48905/v1 ◽

2020 ◽

Author(s):

Madoka V. KRICK ◽

Erick DESMARAIS ◽

Athanasios SAMARAS ◽

Elise GUERET ◽

Arkadios DIMITROGLOU ◽

...

Keyword(s):

Stress Response ◽

Cytosine Methylation ◽

Acute Stress ◽

Dicentrarchus Labrax ◽

Sea Bass ◽

European Sea Bass ◽

Nucleated Red Blood Cells ◽

Genome Wide ◽

A Genome ◽

The Brain

Abstract Background: While the stress response inspired genome-wide epigenetic studies in vertebrate models, it remains mostly ignored in fish. We modified the epiGBS (epiGenotyping By sequencing) technique to explore changes in genome-wide cytosine methylation to a repeated acute stress challenge in the nucleated red blood cells (RBCs) of the European sea bass (Dicentrarchus labrax). This species is widely studied in both the natural and farmed environments, including issues regarding health and welfare.Results: We retrieved 501,108,033 sequencing reads after trimming, with a mean mapping efficiency of 73.0% (unique best hits). Fifty-seven differentially methylated cytosines (DMCs) close to 51 distinct stress-related genes distributed on 17 of 24 linkage groups (LGs) were detected between RBCs of pre- and post-stress individuals. Literature surveys indicated that thirty-eight of these genes were previously reported as differentially expressed in the brain of zebrafish, most of them involved in stress coping differences. DMC-related genes associated to the Brain Derived Neurotrophic Factor, a protein that favors stress adaptation and fear memory, are especially relevant.Conclusion: We provide an improved epiGBS protocol with increased multiplexing and sequencing capacities that offer new opportunities to improve data acquisition and to investigate important biological processes at a genome-wide level, such as the stress response. Minimally invasive RBCs deserve more attention to investigate the epigenetic response to stress without sacrificing fish.

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