Sexual Maturity Promotes Yolk Precursor Synthesis and Follicle Development in Hens via Liver-Blood-Ovary Signal Axis

Several reproductive hormones were reported to be involved in regulating egg yolk precursor synthesis in chickens; however, the mechanism that shows how the liver-blood-ovary signal axis works in relation to age changes has not been reported yet. Therefore, in this study, we observe the morphology and histology of the liver and ovary and determine the serum biochemical parameters and the expression abundance of the critical genes from d90 to 153. Results show that the body weight and liver weight were significantly increased from d132, while the ovary weight increased from d139. Aside from the increase in weight, other distinct changes such as the liver color and an increased deposition of large amounts of yolk precursors into the ovarian follicles were observed. On d139, we observed small fatty vacuoles in the hepatocytes. The results of serum biochemical parameters showed a significant increase in the estradiol (E2) level, first on d125, and then it reached its peak on d132. Meanwhile, the levels of follicle-stimulating hormone (FSH) increased initially and then remained at a high level from d146 to d153, while the levels of luteinizing hormone (LH) increased significantly on d132 and reached the top level on d153. Moreover, the levels of lecithin (LEC), vitellogenin (VTG), very low density lipoprotein y (VLDLy), triglyceride (TG), and total cholesterol (TC) were significantly increased at d125 and were close from d146 to d153. The mRNA and protein expression of estrogen receptor-alpha (ER-α) and E2 levels in the liver and serum, respectively, showed similar changes. Moreover, with reference to an increase in serum E2 level, the mRNA expression of genes related to yolk precursor synthesis (very low density apolipoprotein-II, ApoVLDL-II) and vitellogenin-II (VTG-II), lipogenesis (fatty acid synthase, FAS), and lipid transport (microsomal triglyceride transport protein, MTTP) in the liver showed up-regulation. These results suggest that the correlation between liver-blood-ovary alliances regulate the transport and exchange of synthetic substances to ensure synchronous development and functional coordination between the liver and ovary. We also found that E2 is an activator that is regulated by FSH, which induces histological and functional changes in the hepatocytes through the ER-α pathway.

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Effect of dietary vitamin D levels on growth, serum biochemical parameters, lipid metabolism enzyme activities, fatty acid synthase and hepatic lipase mRNA expression for orange‐spotted grouper ( Epinephelus coioides ) in growth mid‐stage

Aquaculture Nutrition ◽

10.1111/anu.13212 ◽

2021 ◽

Author(s):

Shuqing He ◽

Mingyan Ding ◽

Gyan Watson Ray ◽

Qihui Yang ◽

Beiping Tan ◽

...

Keyword(s):

Biochemical Parameters ◽

Fatty Acid Synthase ◽

Hepatic Lipase ◽

Dietary Vitamin ◽

Epinephelus Coioides ◽

Serum Biochemical Parameters ◽

Vitamin D Levels ◽

Serum Biochemical ◽

Metabolism Enzyme ◽

Dietary Vitamin D

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Effects of different dietary lipid sources on tissue fatty acid composition, serum biochemical parameters and fatty acid synthase of juvenile mud crabScylla paramamosain(Estampador 1949)

Aquaculture Research ◽

10.1111/are.12547 ◽

2014 ◽

Vol 47 (3) ◽

pp. 887-899 ◽

Cited By ~ 13

Author(s):

Juan Zhao ◽

Xiaobo Wen ◽

Shengkang Li ◽

Dashi Zhu ◽

Yuanyou Li

Keyword(s):

Fatty Acid Composition ◽

Fatty Acid ◽

Acid Composition ◽

Biochemical Parameters ◽

Fatty Acid Synthase ◽

Dietary Lipid ◽

Serum Biochemical Parameters ◽

Tissue Fatty Acid Composition ◽

Tissue Fatty Acid ◽

Serum Biochemical

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Effect of Calf Gender on Milk Composition, Reproductive Hormones and Serum Biochemical Parameters of Female Dromedary Camel

International Journal of Veterinary Science ◽

10.47278/journal.ijvs/2020.004 ◽

2021 ◽

Vol 10 (1) ◽

pp. 47-50

Keyword(s):

Biochemical Parameters ◽

Milk Composition ◽

Reproductive Hormones ◽

Dromedary Camel ◽

Biochemical Profile ◽

Serum Biochemical Parameters ◽

Dromedary Camels ◽

Milk Samples ◽

Serum Biochemical ◽

Serum Biochemical Profile

Arabian dromedary camels received little interest compared to other domestic animals. Literature data about factors affecting camel's milk composition and serum biochemical profile are limited and not sufficient. In the present study a total of 60 milk samples (Three milk samples were collected from each animal for three consecutive days) and 20 blood samples were collected individually from 20 clinically healthy female dromedary camels at first month of lactation to study the effect of calf gender on milk composition, some hormonal and serum biochemical profile. Animals were grouped into two groups, A and B. Group A contained 10 females dromedary camels that gave birth to male calves and Group B contained 10 females dromedary camels that gave birth to female calves. All samples were analyzed, and the result revealed that calf gender has non-significant effect (P>0.05) on milk composition, serum progesterone, cortisol, calcium and magnesium levels. While significant effect but not sharp (P=0.02) were observed on serum phosphorus levels and high significant effect were recorded on serum estrogen level (P<0.0001).Since such study are rare inEgypt, the data presented, could be used as a reference guide for the effect of calf gender on milk composition, reproductive hormones and serum biochemical parameters of female dromedary camel during early lactation.

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Effect of dietary antibacterial peptide and zinc-methionine on performance and serum biochemical parameters in piglets

Czech Journal of Animal Science ◽

10.17221/341/2009-cjas ◽

2011 ◽

Vol 56 (No. 1) ◽

pp. 30-36 ◽

Cited By ~ 24

Author(s):

J.H. Wang ◽

C.C. Wu ◽

J. Feng

Keyword(s):

Biochemical Parameters ◽

Average Daily Gain ◽

Density Lipoprotein ◽

Antibacterial Peptide ◽

Control Group ◽

Serum Biochemical Parameters ◽

Peptide Group ◽

Zinc Methionine ◽

Serum Biochemical ◽

Daily Gain

The objective of this study was to evaluate the effect of dietary supplementation of antibacterial peptide and zinc methionine on performance and some serum biochemical parameters of weaned piglets. Rongchang male piglets (28 days of age, initial weight 8.4 ± 0.65 kg) were used. All piglets were randomly allotted to four diets including the control group, the antibacterial peptide (AP) group containing 10 mg antibacterial peptide/kg diet, the zinc methionine (Zn-Met) group with 1 200 zinc methionine/kg diet (equal to 200 mg Zn<sup>2+</sup>/kg diet), the zinc methionine/antibacterial peptide (Zn/AP) group containing 10 mg antibacterial peptide and 1 200 mg zinc methionine/kg diet, respectively, in a 4-week feeding experiment. Each of these groups consisted of six replications with 8 pigs per replication. Average daily gain (ADG) and average daily feed intake (ADFI) both improved in the antibacterial peptide group (P < 0.05, P < 0.05), zinc methionine group (P < 0.05, P < 0.05) and zinc methionine/antibacterial peptide group (P < 0.01, P < 0.05), as compared with the control. Dietary zinc methionine also significantly reduced the diarrhoea ratio of piglets (P < 0.05). The serum immunoglobulin G (IgG) and superoxide dismutase (SOD) were increased (P < 0.05) and total cholesterol (TC) decreased (P < 0.05) in the antibacterial peptide group and zinc-methionine group, the high density lipoprotein (HDL) increased (P < 0.05) in the zinc methionine group, as compared with the control. The results indicated that antibacterial peptide and zinc methionine were effective in improving growth performance, enhancing immune function, blood vessel function and antioxidant enzyme activity of piglets.

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